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Author : Kevin Oliver Kramm
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (123 download)
Download or read book Single-molecule Analysis of Transcription Initiation in Archaea and Eukaryotes written by Kevin Oliver Kramm and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Barbara Treutlein
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (864 download)
Download or read book Mechanisms of Eukaryotic Gene Expression on a Single Molecule Level written by Barbara Treutlein and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jing Zhou
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (795 download)
Download or read book Single-molecule Studies on Transcriptional Elongation in Prokaryotes and Eukaryotes written by Jing Zhou and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription, the process of copying genetic information stored in DNA into RNA, is fundamental to life. It is carried out by an extraordinary nano-machine called RNA polymerase (RNAP). Transcriptional elongation, during which RNAP moves along the DNA, adding one nucleotide at a time to the RNA transcript, is highly dynamic and regulated. The motion of RNAP is discontinuous and interrupted by pauses that play an essential role in gene regulation. Fundamental questions regarding the mechanisms of elongation and its modulation by transcription factors, however, remain unclear. In this dissertation, I focus on using high-resolution, optical trapping techniques to study the mechanisms of transcriptional elongation by both prokaryotic and eukaryotic RNA polymerases at the single-molecule level. First, I describe the studies on how the motion of single E.coli RNAP molecules is modulated by two universally conserved, essential transcription factors (NusA and NusG). From individual transcriptional elongation records, the rates of entering pause states, the pause state lifetimes, and the pause-free elongation speeds can all be extracted. By studying the effects of NusA (and NusG) on these kinetic rates as a function of the applied load, we were able to develop a quantitative kinetic scheme for elongation and pausing. This model not only explains the functions of NusA/NusG, but also provides insight into the mechanism of transcriptional pausing, which had previously been controversial. Second, a novel optical-trapping assay capable of directly probing elongation by individual eukaryotic RNA polymerase II (RNAPII) molecules will be described. We find that the RNAPII trigger loop, an evolutionarily conserved protein subdomain, not only affects each of the three main phases of elongation, namely: substrate binding, translocation, and catalysis; but also plays a critical role in controlling the fidelity of transcription. Our data also support a Brownian ratchet model for elongation which incorporates a secondary nucleotide binding site.
Author : Michael F. Carey
Publisher : CSHL Press
ISBN 13 : 9780879696351
Total Pages : 684 pages
Book Rating : 4.6/5 (963 download)
Download or read book Transcriptional Regulation in Eukaryotes written by Michael F. Carey and published by CSHL Press. This book was released on 2000 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor. This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.
Author : Barbara Treutlein
Publisher :
ISBN 13 : 9783843905916
Total Pages : 226 pages
Book Rating : 4.9/5 (59 download)
Download or read book Mechanisms of eukaryotic gene expression on a single molecule level: From transcription initiation to nucleosome remodeling written by Barbara Treutlein and published by . This book was released on 2012 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Athanasios Papavassiliou
Publisher : International Thomson Publishing Services
ISBN 13 :
Total Pages : 388 pages
Book Rating : 4.F/5 ( download)
Download or read book Transcription Factors in Eukaryotes written by Athanasios Papavassiliou and published by International Thomson Publishing Services. This book was released on 1997 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Robert J. White
Publisher : Springer Science & Business Media
ISBN 13 : 3662035189
Total Pages : 274 pages
Book Rating : 4.6/5 (62 download)
Download or read book RNA Polymerase III Transcription written by Robert J. White and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph reviews and summarizes the substantial body of work that has been published on the transcription by polymerase III over the past 5 years. Progress in this field has been very rapid since 1993, and this new edition incorporates all the recent developments and offers the reader a highly detailed analysis of the current state of research on this largest and most complex of the eukaryotic RNA polymerases.
Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : SangYoon Chung
Publisher :
ISBN 13 :
Total Pages : 124 pages
Book Rating : 4.:/5 (15 download)
Download or read book Investigation of Bacterial Transcription Using Single Molecule Techniques written by SangYoon Chung and published by . This book was released on 2016 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: The numerous complex molecular processes occurring inside living cells are primarily carried out by proteins and other biopolymers, such as ribonucleic acids (RNA). The identity and quantity of the different proteins and RNA determine the cell's phenotype and changes in response to the environment. Therefore, the internal composition of the cell in terms of the type and concentration of proteins and RNA is tightly regulated. Gene expression is the process of using the DNA sequence information to produce these biopolymers. Transcription, the initial step in gene expression, where one strand of DNA is used as template by the enzyme RNA polymerase (RNAP) for synthesizing a complementary RNA or transcript. Since cell phenotype is mostly determined by transcription, a complex regulatory mechanism exists involving a large number of factors to control the level of transcription of a gene. Although most studies are focused on multiple cycles of either transcription or association of DNA and RNA Polymerase (RNAP) to make RNAP-Promoter open complex (RPO), single round transcription studies are crucial in elucidating the mechanism of sophisticated RNAP-DNA interactions and its kinetics in transcription. In this context, we have developed a novel in vitro quenching based single round transcription assay using single molecule detection. Using this, we could successfully dissect initiation kinetics starting from different initial transcribing stages and found that transcription initiation doesn't follow a sequential model (as commonly believed). Instead, we identified a previously uncharacterized state that is unique to initial transcribing complexes and associated with the backtracked RNAP-DNA complex. Also, we have investigated the size/concentration effects of various osmolytes and macromolecular crowding agents, which mimic the crowded cellular environment, on actively-transcribing RNAP and found enhancement in transcription kinetics by larger crowding agents at the same viscosity.
Author : Oi Kwan Wong
Publisher :
ISBN 13 :
Total Pages : 215 pages
Book Rating : 4.:/5 (476 download)
Download or read book Single-molecule Analysis of Transcription and Transcription Regulation written by Oi Kwan Wong and published by . This book was released on 2001 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : J. Robin Harris
Publisher : Springer Nature
ISBN 13 : 3030281515
Total Pages : 657 pages
Book Rating : 4.0/5 (32 download)
Download or read book Macromolecular Protein Complexes II: Structure and Function written by J. Robin Harris and published by Springer Nature. This book was released on 2020-01-14 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book follows on from Volume 83 in the SCBI series (“Macromolecular Protein Complexes”), and addresses several important topics (such as the Proteasome, Anaphase Promoting Complex, Ribosome and Apoptosome) that were not previously included, together with a number of additional exciting topics in this rapidly expanding field of study. Although the first SCBI Protein Complex book focused on soluble protein complexes, the second (Vol. 87)addressed Membrane Complexes, and the third (Vol. 88) put the spotlight on Viral Protein and Nucleoprotein Complexes, a number of membrane, virus and even fibrillar protein complexes have been be considered for inclusion in the present book. A further book is also under preparation that follows the same pattern, in an attempt to provide a thorough coverage of the subject. Chapter 9 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author : Mark Ptashne
Publisher : CSHL Press
ISBN 13 : 9780879696337
Total Pages : 212 pages
Book Rating : 4.6/5 (963 download)
Download or read book Genes & Signals written by Mark Ptashne and published by CSHL Press. This book was released on 2002 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: P. 103.
Author : M. Kates
Publisher : Elsevier
ISBN 13 : 0080860869
Total Pages : 633 pages
Book Rating : 4.0/5 (88 download)
Download or read book The Biochemistry of Archaea (Archaebacteria) written by M. Kates and published by Elsevier. This book was released on 1993-12-13 with total page 633 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last 10 years, considerable information has accumulated on the biochemistry of archaea. In this volume, the subject as a whole is treated in a comprehensive manner. The book brings together recent knowledge concerning general metabolism, bioenergetics, molecular biology and genetics, membrane lipid and cell-wall structural chemistry and evolutionary relations, of the three major groups of archaea: the extreme halophiles, the extreme thermophiles, and the methanogens. Subjects included are: the evolutionary relationship of these microorganisms to all other living cells; special metabolic features of archeaea; protein structural chemistry; cell envelopes; molecular biology in archaea including DNA structure and replication, transcription apparatus, translation apparatus, and ribosomal structure; and a final chapter on the molecular genetics of archaea. This comprehensive scope ensures its usefulness to researchers, and stimulates further study in this rapidly developing field.
Author : Nina Parker
Publisher :
ISBN 13 :
Total Pages : 3250 pages
Book Rating : 4./5 ( download)
Download or read book Microbiology written by Nina Parker and published by . This book was released on 2021-05-03 with total page 3250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs. This is an adaptation of Microbiology by OpenStax. You can access the textbook as pdf for free at openstax.org. Minor editorial changes were made to ensure a better ebook reading experience. Textbook content produced by OpenStax is licensed under a Creative Commons Attribution 4.0 International License.
Author : Hisao Masai
Publisher : Springer
ISBN 13 : 9811069557
Total Pages : 581 pages
Book Rating : 4.8/5 (11 download)
Download or read book DNA Replication written by Hisao Masai and published by Springer. This book was released on 2018-01-22 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the latest trends and future directions of DNA replication research. The contents reflect upon the principles that have been established through the genetic and enzymatic studies of bacterial, viral, and cellular replication during the past decades. The book begins with a historical overview of the studies on eukaryotic DNA replication by Professor Thomas Kelly, a pioneer of the field. The following chapters include genome-wide studies of replication origins and initiation factor binding, as well as the timing of DNA replications, mechanisms of initiation, DNA chain elongation and termination of DNA replication, the structural basis of functions of protein complexes responsible for execution of DNA replication, cell cycle-dependent regulation of DNA replication, the nature of replication stress and cells’ strategy to deal with the stress, and finally how all these phenomena are interconnected to genome instability and development of various diseases. By reviewing the existing concepts ranging from the old principles to the newest ideas, the book gives readers an opportunity to learn how the classical replication principles are now being modified and new concepts are being generated to explain how genome DNA replication is achieved with such high adaptability and plasticity. With the development of new methods including cryoelectron microscopy analyses of huge protein complexes, single molecular analyses of initiation and elongation of DNA replication, and total reconstitution of eukaryotic DNA replication with purified factors, the field is enjoying one of its most exciting moments, and this highly timely book conveys that excitement to all interested readers.
Author : Béatrice Clouet-d'Orval
Publisher : Springer
ISBN 13 : 331965795X
Total Pages : 276 pages
Book Rating : 4.3/5 (196 download)
Download or read book RNA Metabolism and Gene Expression in Archaea written by Béatrice Clouet-d'Orval and published by Springer. This book was released on 2017-10-28 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the regulation of transcription and translation in Archaea and arising insights into the evolution of RNA processing pathways. From synthesis to degradation and the implications of gene expression, it presents the current state of knowledge on archaeal RNA biology in 13 chapters. Topics covered include the modification and maturation of RNAs, the function of small non-coding RNAs and the CRISPR-Cas defense system. While Archaea have long been considered exotic microbial extremophiles, they are now increasingly being recognized as important model microorganisms for the study of molecular mechanisms conserved across the three domains of life, and with regard to the relevance of similarities and differences to eukaryotes and bacteria. This unique book offers a valuable resource for all readers interested in the regulation of gene expression in Archaea and RNA metabolism in general.
Author : Paola Londei
Publisher : Frontiers Media SA
ISBN 13 : 2889741419
Total Pages : 164 pages
Book Rating : 4.8/5 (897 download)
Download or read book Archaeal Ribosomes: Biogenesis, Structure and Function written by Paola Londei and published by Frontiers Media SA. This book was released on 2022-01-24 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:
