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Author : Zheru Zhang
Publisher :
ISBN 13 :
Total Pages : 270 pages
Book Rating : 4.:/5 (479 download)
Download or read book Single Cell Analysis by Capillary Electrophoresis written by Zheru Zhang and published by . This book was released on 2000 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Single Cell Analysis by Capillary Electrophoresis written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Md Abul Fazal
Publisher :
ISBN 13 :
Total Pages : 434 pages
Book Rating : 4.:/5 (733 download)
Download or read book Ultrasensitive One- and Two- Dimensional Capillary Electrophoresis for Single Cell Analysis written by Md Abul Fazal and published by . This book was released on 2005 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Dario Anselmetti
Publisher : John Wiley & Sons
ISBN 13 : 3527626654
Total Pages : 284 pages
Book Rating : 4.5/5 (276 download)
Download or read book Single Cell Analysis written by Dario Anselmetti and published by John Wiley & Sons. This book was released on 2009-03-09 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.
Author : Sheri Joanne Lillard
Publisher :
ISBN 13 :
Total Pages : 298 pages
Book Rating : 4.:/5 (356 download)
Download or read book Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection written by Sheri Joanne Lillard and published by . This book was released on 1996 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 :
Total Pages : 89 pages
Book Rating : 4.:/5 (684 download)
Download or read book DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry written by and published by . This book was released on 1998 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first demonstration of the laser in the 1960's, lasers have found numerous applications in analytical chemistry. In this work, two different applications are described, namely, DNA sequencing with capillary gel electrophoresis and single cell analysis with mass spectrometry. Two projects are described in which high-speed DNA separations with capillary gel electrophoresis were demonstrated. In the third project, flow cytometry and mass spectrometry were coupled via a laser vaporization/ionization interface and individual mammalian cells were analyzed. First, DNA Sanger fragments were separated by capillary gel electrophoresis. A separation speed of 20 basepairs per minute was demonstrated with a mixed poly(ethylene oxide) (PEO) sieving solution. In addition, a new capillary wall treatment protocol was developed in which bare (or uncoated) capillaries can be used in DNA sequencing. Second, a temperature programming scheme was used to separate DNA Sanger fragments. Third, flow cytometry and mass spectrometry were coupled with a laser vaporization/ionization interface.
Author : Jian Chen
Publisher : Academic Press
ISBN 13 : 0323885594
Total Pages : 184 pages
Book Rating : 4.3/5 (238 download)
Download or read book Biosensors for Single-Cell Analysis written by Jian Chen and published by Academic Press. This book was released on 2021-10-26 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization Features case studies that illustrate the application of biosensors for single-cell analysis Considers microfluidic approaches for each single-cell property discussed Explores future directions for single-cell analysis and biosensor technology
Author : Charles Henry
Publisher : Springer Science & Business Media
ISBN 13 : 1597450766
Total Pages : 238 pages
Book Rating : 4.5/5 (974 download)
Download or read book Microchip Capillary Electrophoresis written by Charles Henry and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.
Author : Harvey Abraham Fishman
Publisher :
ISBN 13 :
Total Pages : 444 pages
Book Rating : 4.F/5 ( download)
Download or read book Neurochemical Analysis at the Single-cell Level Using Capillary Electrophoresis written by Harvey Abraham Fishman and published by . This book was released on 1995 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Nga-kit Fung
Publisher :
ISBN 13 :
Total Pages : 308 pages
Book Rating : 4.:/5 (396 download)
Download or read book DNA Sequencing with Capillary Electrophoresis and Single Cell Analysis with Mass Spectrometry written by Nga-kit Fung and published by . This book was released on 1998 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mass detection limit of 20 amol (S/N = 3) of serotonin in a single laser pulse was demonstrated. Determination of serotonin and histamine in individual rat peritoneal mast cells (RPMC) was demonstrated. The intracellular contents of serotonin and histamine were found to vary considerably. No correlation was found between the amount of serotonin and histamine.
Author : Nathalie Bontoux
Publisher : Royal Society of Chemistry
ISBN 13 : 1849732280
Total Pages : 333 pages
Book Rating : 4.8/5 (497 download)
Download or read book Unravelling Single Cell Genomics written by Nathalie Bontoux and published by Royal Society of Chemistry. This book was released on 2010-10-18 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique introduction to the growing field of microfluidics applied to genomics provides an overview of the latest technologies and emphasizes its potential in answering important biological questions. Written by a physicist and a biologist, it offers a more comprehensive view than the previous literature. The book starts with key ideas in molecular biology, developmental biology and microtechnology before going on to cover the specifics of single cell analysis and microfluidic devices for single cell molecular analysis. Review chapters discuss the state-of-the art and will prove invaluable to all those planning to develop microdevices for molecular analysis of single cells. Methods allowing complete analysis of gene expression in the single cell are stressed - as opposed the more commonly used techniques that allow analysis of only a few genes at a time. As pioneers in the field, the authors understand how critical it is for a physicist to understand the biological issues and questions related to single cell analysis, as well for biologists to understand what microfluidics is all about. Aimed predominantly at graduate students, this book will also be of significant interest to scientists working in or affiliated with this field.
Author : Nilhan Gunasekera
Publisher :
ISBN 13 :
Total Pages : 328 pages
Book Rating : 4.:/5 (319 download)
Download or read book Investigations Into the Use of Capillary Electrophoresis for the Analysis of Nuclear Preparations and the Nuclear Localization of Fusion Proteins at the Single Cell Level written by Nilhan Gunasekera and published by . This book was released on 2003 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Xin Zhang
Publisher :
ISBN 13 :
Total Pages : 502 pages
Book Rating : 4.:/5 (521 download)
Download or read book Wavelength-resolved Fluorescence Detection in Capillary Electrophoresis written by Xin Zhang and published by . This book was released on 2002 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Philippe Schmitt-Kopplin
Publisher : Springer Science & Business Media
ISBN 13 : 1588295397
Total Pages : 808 pages
Book Rating : 4.5/5 (882 download)
Download or read book Capillary Electrophoresis written by Philippe Schmitt-Kopplin and published by Springer Science & Business Media. This book was released on 2008-02-25 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics.
Author : James P. Landers
Publisher : CRC Press
ISBN 13 : 9780849324987
Total Pages : 918 pages
Book Rating : 4.3/5 (249 download)
Download or read book Handbook of Capillary Electrophoresis, Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.
Download or read book Protein Analysis at the Single Cell Level by Nonlinear Laser Wave-Mixing Spectroscopy for High Throughput Capillary Electrophoresis Applications written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: SADRI, BEHROKH BAGHERIFAR. Protein Analysis at the Single Cell Level by Nonlinear Laser Wave-Mixing Spectroscopy for High Throughput Capillary Electrophoresis Applications. (Under the direction of William G. Tong and Morteza G. Khaledi) Nonlinear degenerate four-wave mixing is presented as an ultrasensitive optical absorption-based method for detection and measurement of biological samples. Wave-mixing imaging detection technique can localize and quantify biomolecules in single cells and tissue sections with excellent spatial distribution of light absorbed by a target sample. Cellular components can be label-free or labeled with a chromophore or a fluorophore and imaged by wave mixing using a CCD camera. In a 2-D forward-scattering wave-mixing geometry, two overlapping laser beams form interference gratings and transfer their energy to an absorbing medium, creating thermal gratings followed by changes in the refractive index. The probe beam diffracts off these laser- induced gratings to produce the signal beam, which is detected by a CCD camera or a photodiode. A single bio cell can be placed in a glass slide and as the laser beams probe the labeled cellular component, the CCD camera captures wave-mixing signals corresponding to the absorbing cellular components. This nonlinear imaging technique can be used for both live and fixed cells in real time to obtain information on sequential changes in the number, morphology and distribution of cellular components in a single cell. Nonlinear laser wave-mixing spectroscopy coupled with capillary electrophoresis provides a novel ultrasensitive method for single-cell protein analysis. This method is used to detect proteins separated within a single cell. Nonlinear wave mixing has many advantages including quadratic dependency on analyte concentration, high spatial resolution and small sample requirements. Furthermore, wave mixing offers excellent detection sensitivity levels even when using very short optical path lengths, an.
Author : Doh-Hyoung Lee
Publisher :
ISBN 13 :
Total Pages : 440 pages
Book Rating : 4.:/5 (741 download)
Download or read book Fundamental Studies of AC/DC Electrokinetic Phenomena for the Realization of Microchip Capillary Electrophoresis for Single-cell Analysis written by Doh-Hyoung Lee and published by . This book was released on 2011 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt:
