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Author : Hubert Konosuke Suruki
Publisher :
ISBN 13 :
Total Pages : 242 pages
Book Rating : 4.:/5 (338 download)
Download or read book Sequence Specific DNA Binding by ADRI Zing Finger Two written by Hubert Konosuke Suruki and published by . This book was released on 1995 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Pejman Naraghi-Arani
Publisher :
ISBN 13 :
Total Pages : 204 pages
Book Rating : 4.:/5 (315 download)
Download or read book Specific DNA Binding by ADRI Zinc Finger Mutants written by Pejman Naraghi-Arani and published by . This book was released on 1993 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Artem Zykovich
Publisher :
ISBN 13 : 9781124509846
Total Pages : pages
Book Rating : 4.5/5 (98 download)
Download or read book Developing Bind-n-Seq for Identification of C2H2 Zinc Fingers and Other Proteins DNA Binding Properties written by Artem Zykovich and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription factors (TF) play a central role in the regulation of gene expression. Knowledge of TF-DNA binding preferences can significantly improve our understanding of global cellular processes such as differentiation and apoptosis as well as mechanisms that lead to abnormalities in cell fate like cancer. The C2H2 zinc finger is the most common DNA binding domain in the human genome. In addition to being so widespread, there is great interest in studying this domain because it has been successfully employed in targeted genome engineering. Despite years of research on amino acid-nucleotide binding preferences, there is no comprehensive recognition code. Therefore every new zinc finger protein must be tested to empirically determine its DNA binding properties. We developed a new high-throughput method, Bind-n-Seq, for the in vitro analysis of protein-DNA interactions using massively parallel sequencing. The procedure has three steps: (i) binding proteins to randomized oligonucleotide DNA targets, (ii) sequencing the bound oligonucleotides with next-generation technology and (iii) finding motifs among the sequences. There are several key features of Bind-n-Seq that provide advantages over extant methods. Three nucleotide barcodes allow pooling up to 64 binding reactions in a single sequencing run. Extensive library coverage in conjunction with massive sequencing output results in identification of thousands of protein-DNA interactions. The technique is simple and inexpensive. Proteins with long or cooperative binding sites can also be analyzed. We have developed a new motif finding algorithm, Merminator, specifically designed for Bind-n-Seq data. Merminator is based on differential k-mer compositions before and after protein binding. Merminator can be used online at the Bind-n-Seq Source website, where one can process and store data from Bind-n-Seq experiments. The combination of Bind-n-Seq and Merminator allows researchers to investigate protein-DNA interactions in unprecedented depth and breadth.
Author : David S. Latchman
Publisher : Academic Press
ISBN 13 : 0080561039
Total Pages : 507 pages
Book Rating : 4.0/5 (85 download)
Download or read book Eukaryotic Transcription Factors written by David S. Latchman and published by Academic Press. This book was released on 2010-07-28 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription, or the process by which DNA produces RNA, is a central aspect of gene expression. Transcription factors regulate transcription during development and in disease states. As such, it is critical for researchers to gain a good understanding of the relationship between the structure of various families of transcription factors and their function, as well as roles in human disease. Since publication of the Fourth Edition, there have been major advances, notably in the areas of chromatin remodeling and genome-scale analyses. This complete update includes all new coverage of the latest developments, from enabling genomic technologies to studies on the importance of post-translational modifications beyond phosphorylation events. Potential of transcription factors as therapeutic targets in human disease Importance of histone modifications Use of genome-based sequence analysis and high-throughput methods Applications of the chromatin immunoprecipitation (ChIP) assay Transcriptional elongation Regulation by post-translational modifications Regulatory networks and bioinformatics
Author : Wade H. Shafer
Publisher : Springer Science & Business Media
ISBN 13 : 1461303931
Total Pages : 427 pages
Book Rating : 4.4/5 (613 download)
Download or read book Masters Theses in the Pure and Applied Sciences written by Wade H. Shafer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Masters Theses in the Pure and Applied Sciences was first conceived, published, and disseminated by the Center for Information and Numerical Data Analysis and Synthesis (CINDAS)* at Purdue University in 1957, starting its coverage of theses with the academic year 1955. Beginning with Volume 13, the printing and dis semination phases of the activity were transferred to University Microfilms/Xerox of Ann Arbor, Michigan, with the thought that such an arrangement would be more beneficial to the academic and general scientific and technical community. After five years of this joint undertaking we had concluded that it was in the interest of all concerned if the printing and distribution of the volumes were handled by an international publishing house to assure improved service and broader dissemination. Hence, starting with Volume 18, Masters Theses in the Pure and Applied Sciences has been disseminated on a worldwide basis by Plenum Publishing Corporation of New York, and in the same year the coverage was broadened to include Canadian universities. All back issues can also be ordered from Plenum. We have reported in Volume 39 (thesis year 1994) a total of 13,953 thesis titles from 21 Canadian and 159 United States universities. We are sure that this broader base for these titles reported will greatly enhance the value of this impor tant annual reference work. While Volume 39 reports theses submitted in 1994, on occasion, certain uni versities do report theses submitted in previous years but not reported at the time.
Author :
Publisher :
ISBN 13 :
Total Pages : 372 pages
Book Rating : 4.:/5 (659 download)
Download or read book The Protein Binding Potential of C2H2 Zinc Finger Domains written by and published by . This book was released on 2008 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cys2-His2 (C2H2) zinc finger domains were originally identified as DNA binding domains, and uncharacterized domains are typically assumed to bind DNA. However, a growing body of evidence suggests an important and widespread role for these domains in protein binding. Over 100 C2H2 zinc finger-protein interactions have been described. This study uses common bioinformatics tools to identify sequence features that predict a DNA- or protein-binding function. Several issues, including uncertainties about the full functional capabilities of the zinc fingers, complicated these efforts. Therefore, an unbiased approach which directly examined the potential for zinc fingers to facilitate DNA or protein interactions was used to determine the full functional capabilities of the C2H2 domains in two model proteins, human OLF-1/EBF associated zinc finger (OAZ) protein and Zif268. OAZ contains 30 zinc fingers in six clusters, some of which have been previously indicated in DNA or protein interactions. Zif268 is a well-known DNA binding protein with three C2H2 domains. DNA binding was assessed using a target site selection (CAST) assay, and protein binding was assessed using a yeast two-hybrid assay. Results indicate that clusters known to bind DNA could facilitate specific protein interactions, but clusters known to bind protein did not facilitate specific DNA interactions, indicating that DNA binding is a more restricted function of zinc fingers than has previously beenrecognized. These results also suggest that the role of C2H2 zinc finger domains in protein interactions has probably been underestimated. The implication of these findings for the prediction of zinc finger function is discussed.
Author : Edward Bittar
Publisher : Elsevier
ISBN 13 : 0080536735
Total Pages : 427 pages
Book Rating : 4.0/5 (85 download)
Download or read book Molecular and Cellular Genetics written by Edward Bittar and published by Elsevier. This book was released on 1996-09-24 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: The tools of molecular biology have revolutionised our understanding of gene structure and function and changed the teaching of genetics in a fundamental way. The transition from classical genetics to molecular genetics was initiated by two discoveries. One was the discovery that DNA has a complementary double helix structure and the other that a universal genetic code does exist. Both led to the acceptance of the central dogma that RNA molecules are made on DNA templates. The last twenty years have seen remarkable growth in our knowledge of molecular genetics, most of which is the outcome of recombinant DNA technology. This technology which is not limited to cloning, sequencing, and expression has created a biotechnology industry of its own, the purpose of which is to develop new diagnostic and therapeutic approaches in medicine. Both industries in collaboration with the biomedical community are now engaged in laying down the foundation of molecular medicine. The present volume seeks to provide a coherent account of the new science of molecular genetics. Its content however is by no means exhaustive, partly because of the publication explosion but more because of space restrictions. A rudimentary knowledge of genetics on the reader's part is assumed. Quite understandably, considerable emphasis is placed on major technical advances but not without expounding numerous new ideas and phenomena including alternative splicing, POR, DNA methylation, genomic imprinting, and so on.
Author :
Publisher :
ISBN 13 :
Total Pages : 1860 pages
Book Rating : 4.:/5 (319 download)
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ying Zhou
Publisher :
ISBN 13 :
Total Pages : 418 pages
Book Rating : 4.:/5 (862 download)
Download or read book Studies of the Metal Binding Properties and DNA Recognition Mode of the Unusual Zinc Fingers in Poly(ADP-ribose) Polymerase-1 and the Investigation of Its Interaction with Apoptosis Inducing Factor (AIF) written by Ying Zhou and published by . This book was released on 2009 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: Poly(ADP-ribosyl)ation, a covalent modification of proteins catalyzed by poly(ADP-ribose) polymerases (PARPs), plays a crucial role in regulating DNA repair, DNA replication, and cell death. Poly(ADP-ribose) Polymerase-1 (PARP-1) is a nuclear zinc-finger DNA-binding protein that is the most extensively studied member of the PARP family. The activation of PARP-1 depends on the N-terminal DNA-binding domain, which consists of two unusually long zinc finger-like motifs (termed FI and FII) of the form CX2CX28/30HX2C and a newly discovered zinc-ribbon motif (FIII). Though zinc is indispensible for PARP-1 activity, the metal binding affinities of the unusual zinc fingers of PARP-1 is not yet known. In this dissertation, the second zinc finger of PARP-1 was used as a model peptide to study the binding properties of several divalent metal ions (Co2+, Cd2+, Zn2+, and Pb2+). Metal-induced protein folding was investigated by circular dichroism, and the effects of the metal ions on PARP-1 activity were investigated by poly(ADP-ribosyl)ation activity assays. This study represents the first detailed biochemical characterization of the PARP zinc fingers. The functional role of each zinc finger in DNA damage recognition is critical for understanding how PARP-1 is involved in DNA repair. Thus, we constructed a series of PARP-1 zinc finger variant proteins and investigated their DNA binding properties and their effects on PARP activity. Using a combination of southwestern blotting and activity assays, we demonstrated that FII is more important for DNA binding, while FI and FIII seem to facilitate PARP activity. The DNA sequence-independent binding properties of PARP-1 were further characterized using DNA probes bearing defined secondary structures. Together, our results indicate that the zinc fingers help position the enzyme at specific DNA damage sites, and also help to activate the catalytic domain upon DNA binding. PARP-1 is involved in caspase-independent apoptosis, and the translocation of apoptosis inducing factor (AIF) out of the mitochondrial matrix has been shown to require PARP-1 activity. However, it is not readily apparent how the catalytic activity of PARP-1 (a nuclear protein) triggers the release of AIF from the mitochondrial matrix. In an attempt to understand the relationship between PARP-1 activity and caspase-independent apoptosis, we demonstrate here that AIF is an in vitro protein substrate for PARP-1. The possible implications of this finding will be discussed.
Author :
Publisher : Academic Press
ISBN 13 : 0080863388
Total Pages : 359 pages
Book Rating : 4.0/5 (88 download)
Download or read book Progress in Nucleic Acid Research and Molecular Biology written by and published by Academic Press. This book was released on 1995-05-25 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: FROM THE REVIEWS OF THE PREVIOUS VOLUMES: "In perusing these chapters, I found much of interest. It is worth investigating." - P. Bricknell in BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY. "Full of interest not only for the molecular biologist-for whom the numerous references will be invaluable-but will also appeal to a much wider circle of biologists, and in fact to all those who are concerned with the living cell." -BRITISH MEDICAL JOURNAL. * * Provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology * Contributions from leaders in their fields * Abundant references
Author : Sir John Kendrew
Publisher : John Wiley & Sons
ISBN 13 : 1444313843
Total Pages : 1201 pages
Book Rating : 4.4/5 (443 download)
Download or read book The Encylopedia of Molecular Biology written by Sir John Kendrew and published by John Wiley & Sons. This book was released on 2009-07-06 with total page 1201 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Encylopaedia of Molecular Biology is a truly unique work of reference. 6000 definitions cover the entire spectrum of molecular life science The complete one-volume guide to understanding the way molecular biology is transforming medicine and agriculture Long and short entries written by over 300 of the world's finest researchers For rapid research or detailed study ... this is the A to Z of the New Biology
Author : Zeno Simon
Publisher : CRC Press
ISBN 13 : 9780849353987
Total Pages : 356 pages
Book Rating : 4.3/5 (539 download)
Download or read book Specific Interaction and Biological Recognition Processes written by Zeno Simon and published by CRC Press. This book was released on 1993-06-16 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specific Interaction and Biological Recognition Processes is devoted to two major aspects of biological processes: specificity in biological recognition and the recognition processes themselves. Topics covered in specificity include the theoretical basis for specificity in biological recognition; the thermodynamic and chemical equilibrium background; and consideration of the relationship between size of combining sites and specificity. The use of semi-emperical potentials for calculating interaction energies and the potential of quantum chemistry methods for calculating receptor-effector affinities are also discussed. The various recognition processes described include DNA replication, transcription, translation, enzymatic reactions, transmembrane transport processes, mechanisms of action of hormones and other chemical messengers, and self-nonself recognition in immunology. Specific Interaction and Biological Recognition Processes will be a useful reference for molecular biologists, biochemists, enzymologists, immunologists oncologists, pharmaceutical researchers, and others interested in the topic.
Author : Ashok K. Chauhan
Publisher : I. K. International Pvt Ltd
ISBN 13 : 9380026374
Total Pages : 1310 pages
Book Rating : 4.3/5 (8 download)
Download or read book A Textbook of Molecular Biotechnology written by Ashok K. Chauhan and published by I. K. International Pvt Ltd. This book was released on 2009 with total page 1310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Textbook of Molecular Biotechnology covers an amazing range of topics from the basic structure of the cell and diversity of microorganisms to the latest techniques in the field of biotechnology. Various topics have been included for the benefit of graduate and postgraduate students. In addition, the book will be of immense help for the researchers and can be used as a laboratory manual for various biotechnological techniques. A number of reputed subject experts, scientists, academicians, and researchers have contributed their chapters to this volume. This book describes the role of basic biotechnological tools in various spheres of human society, namely, agriculture, nutraceuticals, pharmaceuticals, nanobiotechnology, proteomics, metagenomics and Intellectual Property rights.
Author : Thomas A. Steitz
Publisher : CUP Archive
ISBN 13 : 9780521414890
Total Pages : 108 pages
Book Rating : 4.4/5 (148 download)
Download or read book Structural Studies of Protein-Nucleic Acid Interaction written by Thomas A. Steitz and published by CUP Archive. This book was released on 1993-09-16 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this 1993 text, Nobel Prize winner Professor Steitz reviews the wide-ranging research in structural studies of DNA-binding proteins and their complexes with DNA. The author clearly and concisely describes the uses of techniques in molecular genetics, DNA synthesis, protein crystallography and nuclear magnetic response.
Author : Henri Heslot
Publisher : CRC Press
ISBN 13 : 135108318X
Total Pages : 405 pages
Book Rating : 4.3/5 (51 download)
Download or read book Molecular Biology and Genetic Engineering of Yeasts written by Henri Heslot and published by CRC Press. This book was released on 2018-01-18 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.
Author :
Publisher :
ISBN 13 :
Total Pages : 854 pages
Book Rating : 4.F/5 ( download)
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2002 with total page 854 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 :
Total Pages : 2984 pages
Book Rating : 4.0/5 ( download)
Download or read book Biological & Agricultural Index written by and published by . This book was released on 1997 with total page 2984 pages. Available in PDF, EPUB and Kindle. Book excerpt:
