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Rna Methodologies
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Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Book Synopsis RNA Methodologies by : Robert E. Farrell
Download or read book RNA Methodologies written by Robert E. Farrell and published by Academic Press. This book was released on 2005-04 with total page 795 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Book Synopsis RNA研究方法/分离鉴定RNA的实验指导/导读版/RNA methodologies by : Robert E. Farrell
Download or read book RNA研究方法/分离鉴定RNA的实验指导/导读版/RNA methodologies written by Robert E. Farrell and published by . This book was released on 2005 with total page 767 pages. Available in PDF, EPUB and Kindle. Book excerpt: 原版引进科爱传播生命科学
Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Academic Press. This book was released on 2009-08-31 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center Includes classic and contemporary techniques useful for all labs
Book Synopsis RNA Methodologies by : Robert E. Farrell, Jr.
Download or read book RNA Methodologies written by Robert E. Farrell, Jr. and published by Academic Press. This book was released on 2017-08-11 with total page 876 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise. Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies Presents comprehensive, cutting-edge information covering all aspects of working with RNA Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application Presents multiple avenues for addressing the same experimental goals
Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2022-11-22 with total page 962 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology Builds from basic information on RNA techniques to in-depth protocols for specific applications Features new chapters on RNA sequencing and RNA in situ hybridization Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics
Book Synopsis RNA Methodologies by : Bozzano G Luisa
Download or read book RNA Methodologies written by Bozzano G Luisa and published by Academic Press. This book was released on 2012-12-02 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Studies cellular regulation using RNA as a parameter of Gene Expression * Offers RNA isolation strategies * Explains proper handling, storage, and manipulation of RNA
Download or read book RNA Interference written by Dr. Tim Doran and published by CABI. This book was released on 2009 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.
Download or read book RNA written by Henrik Nielsen and published by Humana Press. This book was released on 2010-12-10 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of “gene X” and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular BiologyTM series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it.
Download or read book RNA Detection written by Imre Gaspar and published by Humana Press. This book was released on 2017-11-15 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.
Download or read book RNA Modifications written by Mary McMahon and published by Humana. This book was released on 2022-06-18 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book describes some of the most recent advances and up-to-date methodologies to detect, quantify, analyze, and elucidate the biological function of different types of RNA modifications. Importantly, the methodologies and tools described herein can be applied to a wide variety of organisms and can be used to address biological and clinical questions. Beginning with a section on bioinformatics tools, the collection continues with sections on detecting RNA modifications using Nanopore direct RNA sequencing, next-generation sequencing approaches, qPCR- and molecular biology-based methods, mass spectrometry- and NMR-based methods, as well as approaches to assess kinetics, determinants, and functions of RNA modifications. Written for the highly successful Methods in Molecular Biology series style, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Modifications: Methods and Protocols serves as an ideal guide for those working directly in the fields of epitranscriptomics and post-transcriptional gene regulation, as well as for scientists and clinicians interested in bioinformatic tools to study RNA modifications and techniques to dissect their roles in physiology and disease. /divChapter 20 is available open access under a CC BY 4.0 license.
Book Synopsis Handbook of RNA Biochemistry by : Roland K. Hartmann
Download or read book Handbook of RNA Biochemistry written by Roland K. Hartmann and published by John Wiley & Sons. This book was released on 2015-06-22 with total page 1370 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.
Book Synopsis RNA Sequence, Structure, and Function: Computational and Bioinformatic Methods by : Jan Gorodkin
Download or read book RNA Sequence, Structure, and Function: Computational and Bioinformatic Methods written by Jan Gorodkin and published by Humana. This book was released on 2014-03-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes. However, a long series of striking discoveries, from RNA's ability to carry out catalytic function, to discovery of riboswitches, microRNAs and other ribo-regulators performing critical tasks in essentially all living organisms, has created a burgeoning interest in this primordial component of the biosphere. However, the structural characteristics and evolutionary constraints on RNA molecules are very different from those of proteins, necessitating development of a completely new suite of informatic tools to address these challenges. In RNA Sequence, Structure, Function: Computational and Bioinformatic Methods, expert researchers in the field describe a substantial and relevant fraction of these methodologies from both practical and computational/algorithmic perspectives. Focusing on both of these directions addresses both the biologist interested in knowing more about RNA bioinformatics as well as the bioinformaticist interested in more detailed aspects of the algorithms. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results. Thorough and intuitive, RNA Sequence, Structure, Function: Computational and Bioinformatic Methods aids scientists in continuing to study key methods and principles of RNA bioinformatics.
Download or read book RNA Editing written by Ernesto Picardi and published by Humana. This book was released on 2021-08-13 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview about main RNA editing mechanisms, focusing on their functions in physiological as well as pathological conditions. Chapters guide readers through state- of-the art methodologies to investigate RNA editing through wet and dry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Editing: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis RNA Bioinformatics by : Ernesto Picardi
Download or read book RNA Bioinformatics written by Ernesto Picardi and published by Humana. This book was released on 2016-09-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview of RNA bioinformatics methodologies, including basic strategies to predict secondary and tertiary structures, and novel algorithms based on massive RNA sequencing. Interest in RNA bioinformatics has rapidly increased thanks to the recent high-throughput sequencing technologies allowing scientists to investigate complete transcriptomes at single nucleotide resolution. Adopting advanced computational technics, scientists are now able to conduct more in-depth studies and present them to you in this book. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible bioinformatics protocols, and key tips to avoid known pitfalls. Authoritative and practical, RNA Bioinformatics seeks to aid scientists in the further study of bioinformatics and computational biology of RNA.
Book Synopsis Molecular Biology of RNA by : Masayori Inouye
Download or read book Molecular Biology of RNA written by Masayori Inouye and published by . This book was released on 1987 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology of RNA: New Perspectives provides an overview of the developments in RNA research as well as the approaches, strategies, and methodologies used. Most of the contributing authors in the present volume participated in the Fifth Stony Brook Symposium entitled ""New Perspectives on the Molecular Biology of RNA"" in May 1986. The text is organized into six parts. Part I contains papers dealing with RNA as an enzyme. Part II presents studies on RNA splicing. Part III examines RNA viruses while Part IV focuses on the role of RNA in DNA replication. Part V is devoted to the structure ...
Book Synopsis Plant Circular RNAs by : Luis María Vaschetto
Download or read book Plant Circular RNAs written by Luis María Vaschetto and published by Humana. This book was released on 2022-07-15 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs’ research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field.
