Receptor Signal Transduction Protocols

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Receptor Signal Transduction Protocols

Author : Gary B. Willars
Publisher : Springer Science & Business Media
ISBN 13 : 1592597548
Total Pages : 420 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Receptor Signal Transduction Protocols by : Gary B. Willars

Download or read book Receptor Signal Transduction Protocols written by Gary B. Willars and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of Receptor Signal Transduction Protocols not only has a new editor, but also a greater focus on G-protein-coupled receptors, their properties per se, and their coupling to immediate downstream binding partners—principally, although not exclusively, the heterotrimeric G-proteins. The new edition combines updates of key chapters from the first edition, as well as a large number of new contributions covering key methodologies that have emerged, or been extended to receptor/G-protein research, in the past 5–6 years. In common with many fields, the range of methods used to assess the first steps in signal transduction are continually expanding and methods that might have been considered too specialized five years ago are now sufficiently routine to be included here. Unlike many research areas, where off-the-shelf kits have made research basically foolproof, signal transduction research still requires considerable expertise, and the methods included here are provided by internationally recognized experts in their fields who have many years of experience using the methods they describe. This not only allows each chapter to impart a clear description of the method, but also to furnish invaluable troubleshooting advice for when things do not go entirely according to plan. Once again we would like to thank the Series Editor, John Walker, for the invitation to compile this second edition, and to express our gratitude to all of the authors who have enthusiastically agreed to provide the uniformly excellent contributions.

Receptor Signal Transduction Protocols

Author : Gary Willars
Publisher :
ISBN 13 : 9781489939678
Total Pages : 434 pages
Book Rating : 4.9/5 (396 download)

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Book Synopsis Receptor Signal Transduction Protocols by : Gary Willars

Download or read book Receptor Signal Transduction Protocols written by Gary Willars and published by . This book was released on 2004 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combines updates of key chapters from the first edition with a large number of new contributions on the many successful methodologies that have emerged more recently. The methods are focused primarily on events at the receptor level, including ligand binding, on the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors. The laboratory protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Receptor Signal Transduction Protocols, Second Edition offers both novice and experienced investigators powerful cutting-edge techniques that provide an array of approaches and specific methods that will aid in the understanding of GPCR structure and function

Receptor Signal Transduction Protocols

Author : Gary B. Willars
Publisher : Humana Press
ISBN 13 : 9781617791277
Total Pages : 479 pages
Book Rating : 4.7/5 (912 download)

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Book Synopsis Receptor Signal Transduction Protocols by : Gary B. Willars

Download or read book Receptor Signal Transduction Protocols written by Gary B. Willars and published by Humana Press. This book was released on 2011-06-01 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of the GPCR field and dealt with methods that allow researchers to address many of the important rising issues. Serving as a companion to the previous editions, this volume contains thorough revisions of some core chapters, the complete rewriting of others to encompass new technological developments since the publication of the prior editions, and the commissioning of brand new, cutting-edge chapters to expand on previous coverage. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Receptor Signal Transduction Protocols, Third Edition serves the scientific community with a broad collection of the current, practical methodologies in this highly promising field.

Signal Transduction Protocols

Author : Robert C. Dickson
Publisher : Springer Science & Business Media
ISBN 13 : 1592598161
Total Pages : 331 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Signal Transduction Protocols by : Robert C. Dickson

Download or read book Signal Transduction Protocols written by Robert C. Dickson and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to fit within the confines of this volume, so hard choices as to what to include had to be made.

Methods in Molecular Biology: Receptors signal transduction protocols

Author : John M. Walker
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (84 download)

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Book Synopsis Methods in Molecular Biology: Receptors signal transduction protocols by : John M. Walker

Download or read book Methods in Molecular Biology: Receptors signal transduction protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Transmembrane Signaling Protocols

Author : Hydar Ali
Publisher : Springer Science & Business Media
ISBN 13 : 1597450480
Total Pages : 354 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Transmembrane Signaling Protocols by : Hydar Ali

Download or read book Transmembrane Signaling Protocols written by Hydar Ali and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: The previous edition of Transmembrane Signaling Protocols was published in 1998. Since then the human genome has been completely sequenced and new methods have been developed for the use of microarrays and proteomics to analyze global changes in gene expression and protein profiles. These advances have increased our ability to understand transmembrane signaling processes in much greater detail. They have also simultaneously enhanced our ability to determine the role of a large number of newly identified molecules in signaling events. In addition, novel video microscopy methods have been developed to image transmembrane signaling events in live cells in real time. In view of these major advances, it is time to update the previous edition. Because of the success of that volume, we have chosen to keep the essential character of the book intact. Introductory chapters from experts have been included to provide overall perspective and an overview of recent advances in signal transduction pathways. The individual chapters now include comp- hensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from tra- genic mice, as well as genomic and proteomic approaches to the analysis of transmembrane signaling events. We would like to express our deep gratitude to the coauthors of this publi- tion. We hope that Transmembrane Signaling Protocols, Second Edition will serve as a valuable resource for future progress in the study of signal transd- tion pathways.

Plant Signal Transduction

Author : Thomas Pfannschmidt
Publisher : Humana
ISBN 13 : 9781588299437
Total Pages : 0 pages
Book Rating : 4.2/5 (994 download)

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Book Synopsis Plant Signal Transduction by : Thomas Pfannschmidt

Download or read book Plant Signal Transduction written by Thomas Pfannschmidt and published by Humana. This book was released on 2008-12-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls. Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study

G Protein-coupled Receptor Signaling in Plants

Author : Mark P. Running
Publisher :
ISBN 13 : 9781627035323
Total Pages : 162 pages
Book Rating : 4.0/5 (353 download)

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Book Synopsis G Protein-coupled Receptor Signaling in Plants by : Mark P. Running

Download or read book G Protein-coupled Receptor Signaling in Plants written by Mark P. Running and published by . This book was released on 2013 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.

G Protein-Coupled Receptor Signaling

Author : Mario Tiberi
Publisher : Humana Press
ISBN 13 : 9781493991204
Total Pages : 406 pages
Book Rating : 4.9/5 (912 download)

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Book Synopsis G Protein-Coupled Receptor Signaling by : Mario Tiberi

Download or read book G Protein-Coupled Receptor Signaling written by Mario Tiberi and published by Humana Press. This book was released on 2019-03-01 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening. Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com.

G Protein Signaling

Author : Alan V. Smrcka
Publisher : Springer Science & Business Media
ISBN 13 : 1592594301
Total Pages : 246 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis G Protein Signaling by : Alan V. Smrcka

Download or read book G Protein Signaling written by Alan V. Smrcka and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Alan V. Smrcka presents a collection of cutting-edge methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Among the readily reproducible techniques presented are those for the purification of G proteins and effectors enzymes, assays of these purified G proteins and effector enzymes, and for the study of G proteins interactions with effectors in intact cells. Additional methods are provided for assaying G protein coupled receptor structure, function, and localization, and for studying the physiological roles for endogenous G proteins.

Nuclear G-Protein Coupled Receptors

Author : Bruce G. Allen
Publisher : Humana
ISBN 13 : 9781493955466
Total Pages : 0 pages
Book Rating : 4.9/5 (554 download)

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Book Synopsis Nuclear G-Protein Coupled Receptors by : Bruce G. Allen

Download or read book Nuclear G-Protein Coupled Receptors written by Bruce G. Allen and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters. Methodology to study the subcellular localization and function of GPCRs and other signaling systems is provided, as well as numerous chapters focusing on methods designed to understand signaling mediated by nuclear and other internal GPCRs. Methods are also described to study the formation of second messengers such as cAMP and to study the trafficking of receptors from the cell surface. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nuclear G-Protein Coupled Receptors: Methods and Protocols seeks to serve both professionals and novices with state-of-the-art approaches to characterize what is becoming a common theme in cellular signaling.

ErbB Receptor Signaling

Author : Zhixiang Wang
Publisher : Humana
ISBN 13 : 9781493984206
Total Pages : 0 pages
Book Rating : 4.9/5 (842 download)

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Book Synopsis ErbB Receptor Signaling by : Zhixiang Wang

Download or read book ErbB Receptor Signaling written by Zhixiang Wang and published by Humana. This book was released on 2018-08-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal models and primary cancer cells from patients. After some overviews, the book continues by covering common protocols for studying various aspects of ErbB receptor-mediated cell signaling, newly-developed methods in biomedical research that are also widely-used in the study of ErbB receptor signaling, epidermal growth factor receptor (EGFR) signaling in Drosophila, as well as protocols for studying ErbB receptor signaling in various animal model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy-to-follow, ErbB Receptor Signaling: Methods and Protocols serves as an ideal guide for researchers who are studying the cell signaling mediated by ErbB receptors and other receptor tyrosine kinases.

Signal Transduction

Author : Lewis C. Cantley
Publisher :
ISBN 13 : 9780879699017
Total Pages : 452 pages
Book Rating : 4.6/5 (99 download)

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Book Synopsis Signal Transduction by : Lewis C. Cantley

Download or read book Signal Transduction written by Lewis C. Cantley and published by . This book was released on 2014-05-31 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This textbook provides a comprehensive view of signal transduction, covering both the fundamental mechanisms involved and their roles in key biological processes. It first lays out the basic principles of signal transduction, explaining how different receptors receive information and transmit it via signaling proteins, ions, and second messengers. It then surveys the major signaling pathways that operate in cells, before examining in detail how these function in processes such as cell growth and division, cell movement, metabolism, development, reproduction, the nervous system, and immune function"--

Plant Signal Transduction

Author : Jose R. Botella
Publisher : Humana Press
ISBN 13 : 9781493949595
Total Pages : 241 pages
Book Rating : 4.9/5 (495 download)

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Book Synopsis Plant Signal Transduction by : Jose R. Botella

Download or read book Plant Signal Transduction written by Jose R. Botella and published by Humana Press. This book was released on 2016-08-23 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.

G Protein-Coupled Receptor--Protein Interactions

Author : Susan R. George
Publisher : Wiley-Liss
ISBN 13 :
Total Pages : 362 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis G Protein-Coupled Receptor--Protein Interactions by : Susan R. George

Download or read book G Protein-Coupled Receptor--Protein Interactions written by Susan R. George and published by Wiley-Liss. This book was released on 2005-03-31 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This exciting volume elucidates how GPCRs organize signal transduction and control intracellular activities. Chapters written by the recognized expert in each respective topic offer state-of-the-art updates on the principles and technology, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.

Signal Transduction Protocols

Author : David A. Kendall
Publisher : Springer Science & Business Media
ISBN 13 : 1592595286
Total Pages : 305 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Signal Transduction Protocols by : David A. Kendall

Download or read book Signal Transduction Protocols written by David A. Kendall and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: As our understanding of the biological sciences expands, the bou- aries between traditional disciplines tend to blur at the edges. Physio- gists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and mole- lar biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruction, precious hours can be spent poring over half-described Methods sections with no guarantee of eventual success. The aim of Signal Transduction Protocols has been to get experts with "hands-on" experience in particular techniques to give detailed accounts of experimental protocols in a recipe-type format, which we hope will circumvent the problems of ambiguity often encountered when reading the literature. The techniques described in Signal Transduction Protocols are those that we think will be most useful in addressing questions in the area of receptor-mediated cell signaling, with particular regard to those receptors that are part of the G-protein-linked superfamily. To keep it to a manageable size, we have omitted any reference to electrophysi- ogy and have instead concentrated on more biochemical approaches.

Phospholipid Signaling Protocols

Author : Ian M. Bird
Publisher :
ISBN 13 : 9781592592555
Total Pages : 380 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Phospholipid Signaling Protocols by : Ian M. Bird

Download or read book Phospholipid Signaling Protocols written by Ian M. Bird and published by . This book was released on 1998 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Phospholipid Signaling Protocols, state-of-the-art techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation are detailed by experts intimately experienced in their use. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites, including phosphoinositols, choline metabolites, and fatty acid metabolites. In addition, there is extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA, as well as a description of their localization by immunohistochemistry and immunocytochemistry. Phospholipid Signaling Protocols offers a wide-ranging collection of cutting-edge techniques for the study of signal transduction through phospholipid intermediates and their metabolites. The book is an indispensable reference for both the newcomer and the experienced researcher seeking to expand knowledge of these critical pathways, and strongly complements its companion volumes-R.A.J. Challiss' Receptor Signal Transduction Protocols, D. Bar-Sagi's, Transmembrane Signaling Protocols, and D. A. Kendall and S.J. Hill's Signal Transduction Protocols-in building a unique library of tried-and-tested protocols relating to the ever expanding signal transduction field.