Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Purification And Characterization Of The Hiv 1 Reverse Transcriptase
Download Purification And Characterization Of The Hiv 1 Reverse Transcriptase full books in PDF, epub, and Kindle. Read online Purification And Characterization Of The Hiv 1 Reverse Transcriptase ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Purification and Characterization of the HIV-1 Reverse Transcriptase by : Anthony Braddock West
Download or read book Purification and Characterization of the HIV-1 Reverse Transcriptase written by Anthony Braddock West and published by . This book was released on 1991 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Purification and Single Molecule Spectroscopy Characterization of HIV-1 Reverse Transcriptase by : Inas El-Shazly
Download or read book Purification and Single Molecule Spectroscopy Characterization of HIV-1 Reverse Transcriptase written by Inas El-Shazly and published by . This book was released on 2006 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structure/function Studies of HIV-1 Reverse Transcriptase by : Seddon Y. Thomas
Download or read book Structure/function Studies of HIV-1 Reverse Transcriptase written by Seddon Y. Thomas and published by . This book was released on 1996 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structure/function Studies of HIV-1 Reverse Transcriptase by : Justin Cole
Download or read book Structure/function Studies of HIV-1 Reverse Transcriptase written by Justin Cole and published by . This book was released on 1996 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Characterization of the RNase H Activity of HIV-1 Reverse Transcriptase by : Xinyi Zhan
Download or read book Characterization of the RNase H Activity of HIV-1 Reverse Transcriptase written by Xinyi Zhan and published by . This book was released on 1993 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Characterization of HIV-1 Reverse Transcriptase Substrate Specificity by Conformationally Sensitive Fluorescence by : Matthew William Kellinger
Download or read book Characterization of HIV-1 Reverse Transcriptase Substrate Specificity by Conformationally Sensitive Fluorescence written by Matthew William Kellinger and published by . This book was released on 2010 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: We have engineered a mutant of HIV Reverse Transcriptase that can be fluorescently labeled by covalent attachment of the environmentally sensitive fluorophore 7-diethylamino-3-((((2-maleimidyl)ethyl)amino)carbonyl)coumarin (MDCC). The result is a polymerase that is kinetically indistinguishable from the wild-type enzyme, but provides a signal to monitor changes in enzyme structure that result from conformational changes induced by substrate binding. Using this system, we have expanded the kinetic model governing nucleotide binding to include an enzymatic isomerization following initial nucleotide binding. In doing so, we define the role of induced-fit in nucleotide specificity and mismatch discrimination. Additionally, we have characterized the kinetics governing the specificity and discrimination of several widely administered Nucleotide Reverse Transcriptase Inhibitors (NRTI's) used to combat HIV infection including 3TC (Lamivudine), FTC (Emtricitabine), and AZT (Zidovudine) for the wild-type polymerase and mutants with clinical resistance to these compounds. Our findings resolve the apparent tighter binding of these inhibitor compounds compared to the correct nucleotide by showing that the affinity for the correct nucleotide is stronger than the inhibitors. The apparent weaker binding of the correct nucleotide is a result of a incomplete interpretation of binding data that fails to account for the importance of the reverse rate of the conformational change. The apparent Kd (Kd, app) measurements for correct nucleotide estimates Km rather than Kd because nucleotide binding does not reach equilibrium. The conformationally sensitive enzyme has also been used to characterize the kinetics governing DNA association. We show that DNA binding is governed by a two-step process where a fast initial association is followed by a second, slow isomerization that is off the pathway for nucleotide binding and incorporation. Finally, we have implemented single molecule techniques using fluorophore labeled nucleotides to study the effects of AZT incorporation on the DNA translocation dynamics of the polymerase. We find that primer termination with AZT results in DNA that fails to translocate, therefore occluding the next nucleotide from binding. This shift in translocation equilibrium exposes the newly formed phosphodiester bond to ATP- or pyrophosphate-mediated AZT excision; thereby rescuing productive polymerization. This finding represents the first kinetic measurement of DNA translocation by a polymerase.
Book Synopsis Biochemical Characterization of Clade B and Non-B HIV-1 Reverse Transcriptase by : Jacqueline A. Flores
Download or read book Biochemical Characterization of Clade B and Non-B HIV-1 Reverse Transcriptase written by Jacqueline A. Flores and published by . This book was released on 2017 with total page 43 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two classes of drugs: nucleos(t)ide reverse transcriptase inhibitors (NRTIs) and non-nucleoside RT inhibitors (NNRTIs) target HIV-1 RT, an enzyme required for HIV replication. The first generation NNRTIs (nevirapine and efavirenz) have been extensively used worldwide. However, they have a low genetic barrier for resistance. The second generation NNRTI, Rilpivirine (RPV) is effective against viruses that are resistant to nevirapine and efavirenz. RPV has not been used against subtype C HIV-1, which accounts for ~50% of all infections. Recent clinical trials have shown that patients with subtype C are more likely to fail RPV-based treatment. However, the mechanism of RPV-failure has not been determined. To get insight into RPV-failure, RTs from patient isolates were cloned and biochemically characterized using pre-steady state kinetics to determine: 1) the impact of genetic variation on HIV-1 replication, and 2) the effect of a known RPVresistance mutation (p66[subscript E138K-M184I]/p51[subscript E138K]) on the initiation of reverse transcription, the first step of HIV replication. The results showed that subtype C HIV-1 RT has lower DNA and dNTP binding affinities, and RPV binding affinity than subtype B HIV-1 RT. The p66[subscript E138K-M184I]/p51[subscript E138K] mutation was found to cause rilpivirine to dissociate from p66[subscript E138KM184I]/p51[subscript E138K] RT 3x faster than wild type RT at initiation - suggesting that RPV may not be an optimal choice for that subtype C infections.
Download or read book HIV-1 Integrase written by Nouri Neamati and published by John Wiley & Sons. This book was released on 2011-08-10 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively covers the mechanisms of action and inhibitor design for HIV-1 integrase. It serves as a resource for scientists facing challenging drug design issues and researchers in antiviral drug discovery. Despite numerous review articles and isolated book chapters dealing with HIV-1 integrase, there has not been a single source for those working to devise anti-AIDS drugs against this promising target. But this book fills that gap and offers a valuable introduction to the field for the interdisciplinary scientists who will need to work together to design drugs that target HIV-1 integrase.
Book Synopsis Purification of HIV-1 Reverse Transcriptase and Identification of Active Site Peptides by Photoaffinity Labeling by : Jennifer Lorraine Cartier
Download or read book Purification of HIV-1 Reverse Transcriptase and Identification of Active Site Peptides by Photoaffinity Labeling written by Jennifer Lorraine Cartier and published by . This book was released on 1992 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Purification of Lentiviral Reverse Transcriptase Enzymes and Characterization of Some Distingushing Features by : Anthony Louis DeVico
Download or read book Purification of Lentiviral Reverse Transcriptase Enzymes and Characterization of Some Distingushing Features written by Anthony Louis DeVico and published by . This book was released on 1992 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Kinetic Characterization of Mutant L289P of Recombinant HIV-1 Reverse Transcriptase by : Tryn Thomas Stimart
Download or read book The Kinetic Characterization of Mutant L289P of Recombinant HIV-1 Reverse Transcriptase written by Tryn Thomas Stimart and published by . This book was released on 1995 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of the Interaction of HIV-1 Reverse Transcriptase and Integrase Proteins by : Eric Adam Hehl
Download or read book Analysis of the Interaction of HIV-1 Reverse Transcriptase and Integrase Proteins written by Eric Adam Hehl and published by . This book was released on 2001 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Membrane Protein Protocols by : Barry S. Selinsky
Download or read book Membrane Protein Protocols written by Barry S. Selinsky and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Download or read book AIDS Bibliography written by and published by . This book was released on 1995-05 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :Katsuhiko S. Murakami Publisher :Springer Science & Business Media ISBN 13 :3642397964 Total Pages :342 pages Book Rating :4.6/5 (423 download)
Book Synopsis Nucleic Acid Polymerases by : Katsuhiko S. Murakami
Download or read book Nucleic Acid Polymerases written by Katsuhiko S. Murakami and published by Springer Science & Business Media. This book was released on 2013-10-22 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerases of all varieties. In other words, this book is for polymerase fans by polymerase fans. Nucleic acid polymerases play a fundamental role in genome replication, maintenance, gene expression and regulation. Throughout evolution these enzymes have been pivotal in transforming life towards RNA self-replicating systems as well as into more stable DNA genomes. These enzymes are generally extremely efficient and accurate in RNA transcription and DNA replication and share common kinetic and structural features. How catalysis can be so amazingly fast without loss of specificity is a question that has intrigued researchers for over 60 years. Certain specialized polymerases that play a critical role in cellular metabolism are used for diverse biotechnological applications and are therefore an essential tool for research.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1995 with total page 1556 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advanced Techniques in Diagnostic Microbiology by : Yi-Wei Tang
Download or read book Advanced Techniques in Diagnostic Microbiology written by Yi-Wei Tang and published by Springer. This book was released on 2018-11-09 with total page 760 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.