Precise Mapping of Hessian Fly and Stripe Rust Resistance Loci in Pacific Northwest Wheat Germplasm (Triticum Aestivum L.)

Download Precise Mapping of Hessian Fly and Stripe Rust Resistance Loci in Pacific Northwest Wheat Germplasm (Triticum Aestivum L.) PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 183 pages
Book Rating : 4.:/5 (111 download)

DOWNLOAD NOW!


Book Synopsis Precise Mapping of Hessian Fly and Stripe Rust Resistance Loci in Pacific Northwest Wheat Germplasm (Triticum Aestivum L.) by : Esra Alwan

Download or read book Precise Mapping of Hessian Fly and Stripe Rust Resistance Loci in Pacific Northwest Wheat Germplasm (Triticum Aestivum L.) written by Esra Alwan and published by . This book was released on 2019 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hessian fly and stripe rust are major pests of spring wheat in the Pacific Northwest and cultivar resistance is a primary breeding goal. Hessian fly (HF) infestations continue to cause significant yield losses in spring wheat in the Pacific Northwest. In wheat, resistance to Hessian fly is usually controlled in a gene for gene manner, similar to other pathosystems. Hessian fly resistance genes in wheat have been failing as a result to the rapid evolutionary pace of the insect. Stripe rust (denoted by Yr) is a destructive foliar disease of wheat casing damage on an annual basis. Most of the Yr named resistance genes have been overcome as a result of the continuing emergence of new virulence races. Genetic resistance remains the most effective and economical approach to minimize yield losses and respond to pathogen evolution. A doubled haploid (DH) mapping population was generated from a cross between two elite spring wheat lines; 'WA8076', and 'HT080158LU'. The DH population was genotyped with 90K SNP markers using the Illumina Infinium platform. Phenotypic assessment was carried out on the DH population for both Hessian fly and stripe rust response. The genotyping efforts resulted in a total 15,236 polymorphic SNP markers used to establish a high-density genetic map. A single Hessian fly resistance gene (HFR) derived from 'WA8076' was detected on the distal region of chromosome 6BS, flanked by two SNP markers IWB71431 and IWB61175 at a distance of 2.6 cM. In addition, a total of 13 genes/QTL were found to be associated with stripe rust resistance. The adapted superior lines with Yr resistance will be directly incorporated in subsequent breeding efforts as a durable source of resistance. The closely linked SNP markers to the resistance loci, identified in this study, will provide an effective tool to accurately identify, select, and integrate the HF and Yr resistance genes into existing wheat cultivars. The highly dense SNP-based genetic map provides useful information for fine mapping and finding candidate genes underlying QTLs as well as marker-assisted breeding. Furthermore, a genome-wide association study (GWAS) we performed for both seedling and adult plant response in a diverse population of winter wheat germplasm. The population was genotyped with the 90K iSelect wheat single nucleotide polymorphism (SNP) array. Our GWAS study identified a total of 26 loci, comprising a range of existing and novel loci associated with stripe rust resistance. Resistance loci in the winter wheat germplasm can be exploited in the development of broadly-effective disease-resistant commercial wheat cultivars.

Molecular Mapping of Stripe Rust Resistance and Freezing Tolerance in Winter Wheat (Triticum Aestivum L.)

Download Molecular Mapping of Stripe Rust Resistance and Freezing Tolerance in Winter Wheat (Triticum Aestivum L.) PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 123 pages
Book Rating : 4.:/5 (81 download)

DOWNLOAD NOW!


Book Synopsis Molecular Mapping of Stripe Rust Resistance and Freezing Tolerance in Winter Wheat (Triticum Aestivum L.) by : Austin Joel Case

Download or read book Molecular Mapping of Stripe Rust Resistance and Freezing Tolerance in Winter Wheat (Triticum Aestivum L.) written by Austin Joel Case and published by . This book was released on 2012 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest

Download Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 193 pages
Book Rating : 4.:/5 (111 download)

DOWNLOAD NOW!


Book Synopsis Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest by : Lu Liu

Download or read book Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest written by Lu Liu and published by . This book was released on 2018 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst), is one of the most devastating and economically important diseases across major wheat growing regions in the world. Growing resistant cultivars is the most effective and environmentally sound method for controlling the disease. This study was conducted to identify resistance genes/QTL in three winter wheat cultivars Madsen, Skiles and Eltan and develop molecular markers for marker-assisted selection. Madsen has shown high-level and durable resistance since its release in 1988 and was identified to have both all-stage resistance (ASR) and high-temperature adult-plant (HTAP) resistance. Two major effect ASR QTL on chromosome arms 1AS and 2AS, one minor ASR QTL on 1BS and two HTAP resistance QTL on 3BS and 6BS were identified in Madsen. Skiles is susceptible to all tested Pst races at seedling stage but shows strong resistance at adult-plant stage with higher resistance level expressed at high temperature, indicating that Skiles has only HTAP resistance against the tested Pst races. Six HTAP resistance QTL were mapped in Skiles, with two major QTL mapped on 3BS and 4BL and four minor QTL on 1BL, 5AL, 6B and 7DL. Markers linked to the QTL were used to select highly resistant breeding lines derived from crosses with Skiles. Eltan has shown variable reactions from moderate susceptibility to resistance in different years since its release in 1990. Five QTL were detected in Eltan, with two major ASR QTL on 2BS and 4BL, one minor ASR on 5BS and two minor HTAP resistance QTL on 6AS and 7BL. The two major ASR QTL became less effective due to the fact that the Pst population has changed to virulent, leading to the resistance reduction in Eltan. By studying these three wheat cultivars with different genes for different types of resistance, it is concluded that the best strategy to achieve high-level and durable resistance is to combine several genes for effective ASR and adequate HTAP resistance. These studies provide the information of useful genes and molecular markers for improving resistance to stripe rust in wheat cultivars.

Mapping High-Temperature Adult-Plant Resistance to Stripe Rust (Puccinia Striiformis) in Spring Wheat (Triticum Aestivum) and Characterization of Their Interaction on a Molecular Level

Download Mapping High-Temperature Adult-Plant Resistance to Stripe Rust (Puccinia Striiformis) in Spring Wheat (Triticum Aestivum) and Characterization of Their Interaction on a Molecular Level PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 126 pages
Book Rating : 4.:/5 (11 download)

DOWNLOAD NOW!


Book Synopsis Mapping High-Temperature Adult-Plant Resistance to Stripe Rust (Puccinia Striiformis) in Spring Wheat (Triticum Aestivum) and Characterization of Their Interaction on a Molecular Level by : Taras Nazarov

Download or read book Mapping High-Temperature Adult-Plant Resistance to Stripe Rust (Puccinia Striiformis) in Spring Wheat (Triticum Aestivum) and Characterization of Their Interaction on a Molecular Level written by Taras Nazarov and published by . This book was released on 2017 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here we report the discovery of precise molecular marker for HTAP resistance locus in wheat cultivar Louise. Using Louise x Penawawa mapping population, coupled with data from survey sequences of wheat genome, linkage mapping and synteny analysis, we developed expressed, cleaved amplified polymorphic sequence (CAPS) marker LPHTAP2B on the short arm of wheat chromosome 2B. It perfectly segregates with the resistant phenotype and explains 62% of phenotypic variance based on disease severity, and 58% based on infection type.

Identification of Quantitative Trait Loci and Molecular Markers for Disease, Insent and Agronomic Traits in Spring Wheat (Triticum Aestivum L.)

Download Identification of Quantitative Trait Loci and Molecular Markers for Disease, Insent and Agronomic Traits in Spring Wheat (Triticum Aestivum L.) PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 157 pages
Book Rating : 4.:/5 (66 download)

DOWNLOAD NOW!


Book Synopsis Identification of Quantitative Trait Loci and Molecular Markers for Disease, Insent and Agronomic Traits in Spring Wheat (Triticum Aestivum L.) by : Arron Hyrum Carter

Download or read book Identification of Quantitative Trait Loci and Molecular Markers for Disease, Insent and Agronomic Traits in Spring Wheat (Triticum Aestivum L.) written by Arron Hyrum Carter and published by . This book was released on 2009 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Multi-location Analysis for the Identification of Quantitative Trait Loci Underlying Disease Resistance Against Cephalosporium Gramineum and Puccinia Striiformis F. Sp. Tritici by Linkage Mapping in Wheat (Triticum Aestivum L.)

Download Multi-location Analysis for the Identification of Quantitative Trait Loci Underlying Disease Resistance Against Cephalosporium Gramineum and Puccinia Striiformis F. Sp. Tritici by Linkage Mapping in Wheat (Triticum Aestivum L.) PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 144 pages
Book Rating : 4.:/5 (876 download)

DOWNLOAD NOW!


Book Synopsis Multi-location Analysis for the Identification of Quantitative Trait Loci Underlying Disease Resistance Against Cephalosporium Gramineum and Puccinia Striiformis F. Sp. Tritici by Linkage Mapping in Wheat (Triticum Aestivum L.) by : Maria Dolores Vazquez

Download or read book Multi-location Analysis for the Identification of Quantitative Trait Loci Underlying Disease Resistance Against Cephalosporium Gramineum and Puccinia Striiformis F. Sp. Tritici by Linkage Mapping in Wheat (Triticum Aestivum L.) written by Maria Dolores Vazquez and published by . This book was released on 2014 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat (Triticum aestivum L.) is one of the major crops produced in the U.S. Pacific Northwest, a region known for its wheat production for international export. Wheat production in the region is threatened by diseases such as Cephalosporium stripe and stripe rust. Cephalosporium stripe is a vascular wilt disease of wheat caused by the persistent soil-borne fungus and suspected necrotrophic pathogen Cephalosporium gramineum Nisikado & Ikata, and is a recurring disease in many localities when susceptible cultivars are grown. Stripe rust, a foliar disease of wheat caused by the air-borne biotrophic fungus Puccinia striformis f. sp. tritici is a disease present in every region around the world where commercial wheat is grown. Attaining durable resistance to stripe rust would greatly benefit wheat producers in the region. Combining stripe rust resistance with resistance to other diseases, such as Cephalosporium stripe, is challenging. Wheat cultivars with high levels of resistance to several diseases are favorable candidates for genetic studies to determine the inheritance of resistance and facilitate the development of a method to genotypically select for disease resistance. Two populations of recombinant inbred lines were developed from 'Tubbs'/'NSA-98-0995' (TxN) and 'Einstein'/'Tubbs' (ExT) with population sizes of 271 and 259 F[subscript (5:6)], respectively. Tubbs is susceptible to stripe rust and Cephalosporium stripe while Einstein and NSA-98-0995 demonstrate moderate to high resistance to both diseases. Both populations were assessed across seven environments (combinations of locations and years) for stripe rust resistance under natural infection and four environments for Cephalosporium stripe resistance under artificial inoculation. The populations were mapped using diversity array technology (DArT) and simple sequence repeat (SSR) markers for quantitative trait loci (QTL) analysis. Results for Cephalosporium stripe resistance was quantitatively inherited with several QTL detected (>5), including some QTL in the same chromosome location in both populations. For stripe rust resistance, seven QTL were identified in the TxN population, suggesting quantitative resistance contributed by several minor genes. In the ExT population two QTL with major effects and with epistatic interactions between them were identified. One of them, a major QTL from Tubbs on chromosome 2AS that may be Yr17, was not expressed in the TxN population or in Tubbs, perhaps owing to suppressor(s). Expression of the 2AS QTL in the ExT population may be due to interaction with the QTL on chromosome 6AL from the resistant parent Einstein or to any other gene in the background of the population. QTL on chromosomes 2AS, 5AL, and 6BS were associated with resistance to both Cephalosporium stripe and stripe rust. These results highlight a complex set of interactions among major genes, minor genes, the presence of different stripe rust races, epistasis, genetic background, and possibly a suppressor of resistance. Results from this study are expected to assist in selecting molecular markers to genotypically select for resistance to these diseases, improving the chances of developing wheat cultivars with durable resistance to both diseases in the future.

QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.)

Download QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.) PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (925 download)

DOWNLOAD NOW!


Book Synopsis QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.) by : Emily Frances Klarquist

Download or read book QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.) written by Emily Frances Klarquist and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Characterization and Genetic Mapping of Leaf Rust (Puccinia Triticina) Resistance Genes Lr2a and Lr46 in Canadian Spring Wheat (Triticum Aestivum) Germplasm

Download Characterization and Genetic Mapping of Leaf Rust (Puccinia Triticina) Resistance Genes Lr2a and Lr46 in Canadian Spring Wheat (Triticum Aestivum) Germplasm PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (135 download)

DOWNLOAD NOW!


Book Synopsis Characterization and Genetic Mapping of Leaf Rust (Puccinia Triticina) Resistance Genes Lr2a and Lr46 in Canadian Spring Wheat (Triticum Aestivum) Germplasm by : Mallorie Lewarne

Download or read book Characterization and Genetic Mapping of Leaf Rust (Puccinia Triticina) Resistance Genes Lr2a and Lr46 in Canadian Spring Wheat (Triticum Aestivum) Germplasm written by Mallorie Lewarne and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Of the fungal diseases that can infect bread wheat (Triticum aestivum L.), leaf rust, caused by Puccinia triticina Eriks. is the most common and widespread. Pyramiding multiple resistance genes in a cultivar using conventional breeding techniques is often expensive and time consuming. Alternatively, marker assisted selection (MAS) allows for accelerated and accurate selection of resistance gene combinations. The objectives of this study were to characterize two leaf rust resistance genes: an adult plant resistance (APR) gene, hypothesized to be Lr46, from wheat line BW278, and a seedling resistance gene, Lr2a, from wheat cultivar Superb. To characterize the APR, two mapping populations derived from BW278 were genotyped with the iSelect 90K wheat SNP array. Both populations were evaluated for leaf rust in inoculated field nurseries for five years. Quantitative trait locus (QTL) analysis revealed two QTL controlling resistance in the BW278/AC Foremost population, one in the region of interest, chromosome 1B and another on chromosome 5A. Two QTL were detected in Superb/BW278, on chromosomes 4B and 5B, however no QTL were detected in the region of interest on 1B. The QTL on 1B in BW278/AC Foremost, designated QLr.mrdc-1B, was tightly linked to both csLV46G22 and DK0900, two markers previously described as tightly linked to the Lr46 locus. Ten SNPs in the QLr.mrdc-1B region were selected for kompetitive allele-specific PCR (KASP) assay design. To characterize Lr2a, two mapping populations derived from Superb (Superb/BW278 & Superb/86ISMN 2137) were genotyped with the iSelect 90 K wheat SNP array, and evaluated with a single race of P. triticina under greenhouse conditions. Two-point linkage analysis between the marker data and phenotypic infection type ratings revealed that the gene mapped to chromosome 2DS in both mapping populations. The linkage maps generated for the two mapping populations had 11 SNP markers in common and displayed collinearity. Seven SNPs that either flanked or co-segregated with Lr2a in Superb/BW278 were selected for KASP assay design. Of the seven markers, kwh740 (Excalibur_c1944_1017) was polymorphic in both populations and displayed clear clusters, making it the most applicable for use in MAS.

Fine Mapping of a Stripe Rust Resistance Gene Transferred from Triticum Monococcum L. Into Hexaploid Wheat Triticum Aestivum L.

Download Fine Mapping of a Stripe Rust Resistance Gene Transferred from Triticum Monococcum L. Into Hexaploid Wheat Triticum Aestivum L. PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 139 pages
Book Rating : 4.:/5 (652 download)

DOWNLOAD NOW!


Book Synopsis Fine Mapping of a Stripe Rust Resistance Gene Transferred from Triticum Monococcum L. Into Hexaploid Wheat Triticum Aestivum L. by : Simarjit Kaur

Download or read book Fine Mapping of a Stripe Rust Resistance Gene Transferred from Triticum Monococcum L. Into Hexaploid Wheat Triticum Aestivum L. written by Simarjit Kaur and published by . This book was released on 2008 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Quantitative Trait Loci Mapping of Rust Resistance and Agronomic Traits in the Doubled Haploid Spring Wheat Population 'HYAYT12-10' × 'GP146'

Download Quantitative Trait Loci Mapping of Rust Resistance and Agronomic Traits in the Doubled Haploid Spring Wheat Population 'HYAYT12-10' × 'GP146' PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (133 download)

DOWNLOAD NOW!


Book Synopsis Quantitative Trait Loci Mapping of Rust Resistance and Agronomic Traits in the Doubled Haploid Spring Wheat Population 'HYAYT12-10' × 'GP146' by : Izabela L. Ciechanowska

Download or read book Quantitative Trait Loci Mapping of Rust Resistance and Agronomic Traits in the Doubled Haploid Spring Wheat Population 'HYAYT12-10' × 'GP146' written by Izabela L. Ciechanowska and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Marker-assisted selection requires the identification of molecular markers associated with major genes and quantitative trait loci (QTL) using linkage analysis. In this study, we used 167 doubled haploid (DH) lines derived from two unregistered spring wheat (Triticum aestivum L.) parental lines that belong to the Canada Western Special Purpose (CWSP) class to map QTLs associated with five traits using inclusive composite interval mapping (ICIM). Using ICIM, least square means phenotype data across 3-4 environments, and a genetic map of 2,676 SNPs out of the wheat 90K SNP array, we identified ten QTLs associated with maturity (4A and 5B), plant lodging (4B, 5A, 5D, and 7D), grain yield (2D), leaf rust (4A) and stem rust (1A and 2B). Each QTL individually accounted for 6.0-22.3% of the phenotypic variance and together accounted for 8.6-38.2% of each trait. QTLs identified for rusts using ICIM had a minor effect (6.0-9.0%) or a major effect (22.3%). Our major effect QTL at 22.3% was discovered on chromosome 2B and contributed to stem rust response. Its physical location has been associated with disease response in previous studies. Results from this study provide additional valuable information to wheat researchers, in particular that the area on chromosome 2B should be considered for future analyses.

Physiologic Races of Strip Rust (Puccinia Striiformis West.) of Wheat (Triticum Aestivum Vill.) in the Pacific Northwest During 1971 and 1972

Download Physiologic Races of Strip Rust (Puccinia Striiformis West.) of Wheat (Triticum Aestivum Vill.) in the Pacific Northwest During 1971 and 1972 PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 108 pages
Book Rating : 4.:/5 (984 download)

DOWNLOAD NOW!


Book Synopsis Physiologic Races of Strip Rust (Puccinia Striiformis West.) of Wheat (Triticum Aestivum Vill.) in the Pacific Northwest During 1971 and 1972 by : Cevdet Dutlu

Download or read book Physiologic Races of Strip Rust (Puccinia Striiformis West.) of Wheat (Triticum Aestivum Vill.) in the Pacific Northwest During 1971 and 1972 written by Cevdet Dutlu and published by . This book was released on 1974 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: The virulence of 44 isolates of stripe rust (Puccinia striiformis West.) from 33 collections made during 1971 and 1972 were characterized on two sets of differential varieties. The varieties in the "Oregon" set were: Cappelle Desprez, Chinese 166, Dippes Triumph, Druchamp, Etiole de Choisy, Flamingo, Gaines, Golden, Ibis, Leda, Michigan Amber, Moro, Omar, Rubis, Suwon 92 x Omar4, and Yamhill. The varieties in the "U. S." set were: Lemhi, Chinese 166, Heines VII, Moro, Suwon 92 x Omar4, Druchamp and Riebesel 47-51, Races were named using a modification of the recently proposed system of decanery numbers. Each race was designated by two values, e.g., OR 106-362, making a dual system of values. The first number (106) represents varieties showing 3 and 4 infection types and the second (362) those with 2, 3 and 4 infection types. Based on this dual system, 19 physiologic races of stripe rust were identified from the 33 collections. Certain races named by the dual system may be closely related and show a shift in virulence over time. Based on the number of races identified in the Pacific Northwest, from a relatively few collections, a great diversity of races in time and space occurred. More than half of the collections were different races. Two major wheat varieties grown in the Pacific Northwest, Gaines and Nugaines, supported 11 out of the 19 races identified. However, only one race, OR 110-110 (Moro race), was associated with Moro wheat while the other varieties supported two or more races. The Pacific Northwest was partitioned into five distinct wheat growing areas based on geographic plus ecological differences. Race OR 106- 106 was the most widely distributed race and was found in all areas except the Upper Columbia Basin. The widespread distribution of this race may be related to its ability to attack a wide range of commercially grown Pacific Northwest wheat varieties. All of the wheat growing areas had one or more races in common, even though 11 of the 19 races were specific for one of the five designated wheat growing areas. The distribution pattern of stripe rust races, therefore, in the Pacific Northwest is neither area specific nor variety dependent. No race has predominated in the population since 1964. However, there was evidence for a seasonal shift in the race make-up of the stripe rust population. In view of the extensive genetic diversity in the stripe rust population in the Pacific Northwest, there is a potential for stripe rust epidemics on old and new wheat varieties. This danger can be reduced by growing varieties with different genetic backgrounds and by maintaining genetic diversity in the varieties of wheat.

Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population

Download Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (141 download)

DOWNLOAD NOW!


Book Synopsis Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population by : Wardah K. Mustahsan

Download or read book Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population written by Wardah K. Mustahsan and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat is a vital cereal crop, providing 20 % of the daily nutritional requirements for consumers worldwide. Although there has been a substantial increase in production and yield gains, food demands will still be on the rise in future decades, and wheat yields must continue to increase rapidly to meet these demands. Grain yield can be inhibited by biotic factors (pathogens), which require the development of resistant varieties against pathogens. Stripe (yellow) rust produces massive yield losses in wheat production, and ample resistance against stripe rust is achieved by pyramiding multiple resistant genes together. Yield also can be improved by characterizing the underlying mechanisms that define yield and yield-related traits. We used breeding technology tools to dissect the genetic architecture of yield-related traits, yield, and stripe rust resistance to identify genomic regions that can improve these traits in wheat germplasm and broaden the genetic resources available for breeders to develop robust breeding germplasm that can improve the profitability and resilience of wheat production. Stripe (yellow) rust, caused by Puccinia striiformis (Pst), is a devastating disease of wheat worldwide. In commercial production, stripe rust reduces forage yield, grain yield and grain quality. Yield losses caused by stripe rust range from 10% to 70%. This study was conducted using two different wheat reference genomes (IWGSC v2.1('Chinese Spring') and 'Jagger') to identify quantitative trait loci (QTL) associated with adult plant field resistance to stripe rust in the hard winter wheat RIL population, 'Overley' x 'Overland' to provide breeders with identified genomic regions associated with quantitative field resistance. Our QTL analysis identified genomic regions on 2AS (2N[superscript v]S translocation), 2BS, 2BL, and 2DL using two different mapping methods (Inclusive Composite Interval Mapping and Multi-Environmental Trial Analysis) that were associated with stripe rust resistance for both infection type and severity using genetic maps from both genomes. There were no meaningful differences in interpreting the data from the two maps. Overley contributed the resistance alleles at the 2AS and 2BL QTLs. Overland contributed the resistance alleles at the 2BS and 2DL QTLs. The 2DL QTL (QYr.hwwg-2D) will be further refined due its environmental stability for resistance. We designed PCR-based SNP marker assays to efficiently identify these genomic regions in breeding populations. Yield improvement can be facilitated by a deeper understanding of the underlying infrastructure of components that determine grain yield. We performed a QTL analysis on the population Overley x Overland, to identify genomic regions in our population can explain the variation in yield and its components in field trials using the two different reference maps. We identified major QTLs in both reference maps for yield (3BL), single kernel weight (4AL), plant height (6AS), grains per spike (4AL), thousand grain weight (4AL), and physiological maturity (2BS). The Jagger map identified more QTLs (40 QTLs) than the IWGSCv2.1 map (30 QTLs). The future direction of these studies will be marker development to utilize these QTLs in yield-improvement breeding programs.

QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population

Download QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (12 download)

DOWNLOAD NOW!


Book Synopsis QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population by : Carmen Johanne Barkley

Download or read book QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population written by Carmen Johanne Barkley and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat (Triticum spp.) is a crop of major economic and dietary importance. In the Pacific Northwest region of the United States, soft white wheat is the primary class of wheat grown and is an important export product for the region. Soft white wheat flour is especially important in producing products such as cookies, crackers, pancakes, noodles and pastry products. The goal of this project was to map quantitative trait loci (QTL) for end-use quality and stripe rust (Puccinia striiformis f. sp. tritici) resistance in a double haploid mapping population derived from a cross between a soft white winter club wheat, ‘Cara’, and a soft white winter lax wheat, ‘Xerpha’. The mapping population of 126 individuals was grown across 12 environments from 2013 to 2017 and rated for stripe rust resistance. In a separate experiment, it was grown in 16 different locations from 2013 to 2016 and phenotyped for 16 end-use quality traits. A linkage map of 23 linkage groups was constructed for the population using 1282 genotyping-by-sequencing (GBS) and KASP markers. QTL analysis revealed 35 significant QTL for stripe rust resistance. Two of the most interesting markers in the stripe rust resistance analysis were IWB38687 and A2927, which were each associated with three separate QTL. Analysis of the end-use quality trait data identified 22 significant QTL. Some of the most significant QTL identified for end-use quality traits had LOD scores well over five and explained over 15% of the variance in the trait they were associated with. Some of the markers identified in this study could be good targets for marker assisted selection in PNW wheat breeding programs, especially because the parents of our mapping population are commonly used in breeding programs in the PNW.

Genetic Interactions Among Resistance Genes in Wheat (Triticum Aestivum L.) and Virulence Genes in the Hessian Fly [Mayetiola Destructor (Say)] in North America and Morocco

Download Genetic Interactions Among Resistance Genes in Wheat (Triticum Aestivum L.) and Virulence Genes in the Hessian Fly [Mayetiola Destructor (Say)] in North America and Morocco PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 238 pages
Book Rating : 4.:/5 (274 download)

DOWNLOAD NOW!


Book Synopsis Genetic Interactions Among Resistance Genes in Wheat (Triticum Aestivum L.) and Virulence Genes in the Hessian Fly [Mayetiola Destructor (Say)] in North America and Morocco by : Mustapha El Bouhssini

Download or read book Genetic Interactions Among Resistance Genes in Wheat (Triticum Aestivum L.) and Virulence Genes in the Hessian Fly [Mayetiola Destructor (Say)] in North America and Morocco written by Mustapha El Bouhssini and published by . This book was released on 1992 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Genetic Interactions Between Resistance in Wheat, Triticum Aestivum L., and Races of Hessian Fly, Mayetiola Destructor (Say).

Download Genetic Interactions Between Resistance in Wheat, Triticum Aestivum L., and Races of Hessian Fly, Mayetiola Destructor (Say). PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 258 pages
Book Rating : 4.:/5 (128 download)

DOWNLOAD NOW!


Book Synopsis Genetic Interactions Between Resistance in Wheat, Triticum Aestivum L., and Races of Hessian Fly, Mayetiola Destructor (Say). by : Jimmy Howell Hatchett

Download or read book Genetic Interactions Between Resistance in Wheat, Triticum Aestivum L., and Races of Hessian Fly, Mayetiola Destructor (Say). written by Jimmy Howell Hatchett and published by . This book was released on 1969 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Development and Use of CDNA-derived SSR Markers for Studying Puccinia Striiformis Populations and Molecular Mapping of New Genes for Effective Resistance to Stripe Rust in Durum Wheat

Download Development and Use of CDNA-derived SSR Markers for Studying Puccinia Striiformis Populations and Molecular Mapping of New Genes for Effective Resistance to Stripe Rust in Durum Wheat PDF Online Free

Author :
Publisher :
ISBN 13 : 9781267671158
Total Pages : pages
Book Rating : 4.6/5 (711 download)

DOWNLOAD NOW!


Book Synopsis Development and Use of CDNA-derived SSR Markers for Studying Puccinia Striiformis Populations and Molecular Mapping of New Genes for Effective Resistance to Stripe Rust in Durum Wheat by : Peng Cheng

Download or read book Development and Use of CDNA-derived SSR Markers for Studying Puccinia Striiformis Populations and Molecular Mapping of New Genes for Effective Resistance to Stripe Rust in Durum Wheat written by Peng Cheng and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Puccinia striiformis, a basidiomycete fungus, produces dikaryotic urediniospores causing stripe rust of wheat, barley, and many grass species. To study its population biology, three cDNA libraries were screened for simple sequence repeats (SSRs) and their flanking sequences were used to design primers. Seventeen primer pairs produced stable polymorphic markers among 28 isolates of the pathogen.

Genetic Characterization of Virulence in a Pacific Northwest Stem Rust Population and Mapping of New Sources of Resistance in Barley

Download Genetic Characterization of Virulence in a Pacific Northwest Stem Rust Population and Mapping of New Sources of Resistance in Barley PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.3/5 (799 download)

DOWNLOAD NOW!


Book Synopsis Genetic Characterization of Virulence in a Pacific Northwest Stem Rust Population and Mapping of New Sources of Resistance in Barley by : Arjun Upadhaya

Download or read book Genetic Characterization of Virulence in a Pacific Northwest Stem Rust Population and Mapping of New Sources of Resistance in Barley written by Arjun Upadhaya and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem rust caused by the biotrophic fungal pathogen Puccinia graminis f. sp. tritici (Pgt) is an important disease of barley and wheat globally. In the last decade, the Pacific Northwest (PNW) region of the United States has experienced an increase in the incidence and severity of stem rust disease. This may be attributed to various factors, including increase in summer temperatures, lack of effective resistance, and early inoculum from sexual hosts. The PNW region's conducive environment and the presence of the sexual hosts, Mahonia and barberry, present an opportunity for the stem rust pathogen to complete its sexual cycle, leading to the emergence of genotypes with novel virulence gene combinations. A total of 100 Pgt isolates collected from barley in eastern Washington during the 2019 growing season were tested for virulence on the two major barley stem rust resistance genes/loci, Rpg1 and the rpg4/5-mediated resistance locus (RMRL) at the seedling stage. Of these isolates, 99% were virulent on cultivar Morex containing the Rpg1 gene, 62% were virulent on Golden Promise transgenic line (H228.2c) carrying a single copy of the Rpg1 gene from Morex, 16% were virulent on the near isogenic line, HQ-1 containing RMRL, and 10% were virulent on barley line Q21861 carrying both Rpg1 and RMRL. This was the first report of Pgt isolates containing virulence on both Rpg1 and RMRL when stacked together, representing the most virulent Pgt population reported on barley worldwide. To identify and map effective resistances against the local virulent isolates, a genome wide association study (GWAS) was conducted on 440 accessions from the World Barley Core Collection (WBCC), genotyped with the 9K Illumina barley iSelect chip and phenotyped with two PNW Pgt isolates at the seedling stage. A total of 10 resistance loci were identified including four novel loci on chromosomes 1H, 2H, 5H, and 6H. On the pathogen side, GWAS was performed using 113 diverse Pgt isolates, collected from the PNW and the Midwest region of the US, to identify virulence and avirulence loci corresponding to the barley stem rust resistance gene Rpg1 and the six wheat stem rust R-genes, Sr5, Sr21, Sr8a, Sr17, Sr9a, and Sr9d. The genotype data for 96 isolates were generated by whole genome shotgun sequencing and for 17 isolates by RNA sequencing. The phenotype data for the Pgt isolates were collected by seedling disease assays on two barley lines, H228.2c and Morex (Rpg1+) and the six wheat stem rust differential lines, Sr5, Sr21, Sr8a, Sr17, Sr9a, and Sr9d. A total of five effector gene candidates were identified at two loci corresponding to the Rpg1 gene. Similarly, 24 putative effector genes were identified at 15 loci corresponding to the six wheat R-genes. Further characterization of these genes will be done to enhance our understanding of host pathogen interactions in both the barley-Pgt and wheat-Pgt pathosystems.