Practical Thin Layer Chromatography

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Thin-Layer Chromatography

Author : Peter E Wall
Publisher : Royal Society of Chemistry
ISBN 13 : 1847552463
Total Pages : 204 pages
Book Rating : 4.8/5 (475 download)

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Book Synopsis Thin-Layer Chromatography by : Peter E Wall

Download or read book Thin-Layer Chromatography written by Peter E Wall and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thin-Layer Chromatography (TLC) is a modern, reliable tool that complements other chromatographic techniques. This book provides a practical guide to the basic principles, procedures and pitfalls on the practical application of TLC. Thin Layer Chromatography: A Modern Practical Approach offers a sequence of chapters following the steps of the technique as the chromatographer would follow them. The chapters provide a choice of sorbent best suited to the separation intended, followed by pre-treatment required for the sample, applying the sample to the sorbent layer, development procedure, visualisation and detection, and finally quantification. Imaging and hyphenation techniques are described. The reasons why recommendations are made for specific and more general methods are covered. The book also provides an overview of some recent developments in the field.

Quantitative Thin-Layer Chromatography

Author : Bernd Spangenberg
Publisher : Springer Science & Business Media
ISBN 13 : 364210729X
Total Pages : 388 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Quantitative Thin-Layer Chromatography by : Bernd Spangenberg

Download or read book Quantitative Thin-Layer Chromatography written by Bernd Spangenberg and published by Springer Science & Business Media. This book was released on 2011-01-03 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.

Practical Thin-Layer Chromatography

Author : Bernard Fried
Publisher : CRC Press
ISBN 13 : 1351422413
Total Pages : 455 pages
Book Rating : 4.3/5 (514 download)

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Book Synopsis Practical Thin-Layer Chromatography by : Bernard Fried

Download or read book Practical Thin-Layer Chromatography written by Bernard Fried and published by CRC Press. This book was released on 2017-11-22 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: Practical Thin-Layer Chromatography provides thorough coverage of the principles, practices, and applications of thin-layer chromatography (TLC) for important sample and compound types. This information is directed specifically at workers in the most active scientific fields.

Practical Thin-Layer Chromatography

Author : Bernard Fried
Publisher : CRC Press
ISBN 13 : 9780849326608
Total Pages : 294 pages
Book Rating : 4.3/5 (266 download)

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Book Synopsis Practical Thin-Layer Chromatography by : Bernard Fried

Download or read book Practical Thin-Layer Chromatography written by Bernard Fried and published by CRC Press. This book was released on 1996-05-29 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Practical Thin-Layer Chromatography provides thorough coverage of the principles, practices, and applications of thin-layer chromatography (TLC) for important sample and compound types. This information is directed specifically at workers in the most active scientific fields.

Paper and Thin Layer Chromatography

Author : Ivor Smith
Publisher : Elsevier
ISBN 13 : 1483279928
Total Pages : 476 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Paper and Thin Layer Chromatography by : Ivor Smith

Download or read book Paper and Thin Layer Chromatography written by Ivor Smith and published by Elsevier. This book was released on 2013-10-22 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatographic & Electrophoretic Techniques, Fourth Edition, Volume I: Paper and Thin Layer Chromatography presents the methods of paper and thin layer chromatography. This book discusses the practical approach in the application of paper and thin layer chromatography techniques in the biological sciences. Organized into 18 chapters, this edition begins with an overview of the clinical aspects related to the detection of those metabolic diseases that can result in serious illness presenting in infancy and early childhood. This text then discusses the three major types of screening for inherited metabolic disorders in which paper or thin-layer chromatography are being used, including screening the healthy newborn population, screening the sick hospitalized child, and screening mentally retarded patients. Other chapters consider the procedures for thin layer chromatography. This book discusses as well the complexity of amino acid mixtures present in natural products. The final chapter deals with the detection of synthetic basic drugs. This book is a valuable resource for chemists and toxicologists.

Applied Thin-Layer Chromatography

Author : Elke Hahn-Deinstrop
Publisher : John Wiley & Sons
ISBN 13 : 3527609857
Total Pages : 330 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Applied Thin-Layer Chromatography by : Elke Hahn-Deinstrop

Download or read book Applied Thin-Layer Chromatography written by Elke Hahn-Deinstrop and published by John Wiley & Sons. This book was released on 2007-02-27 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition of the practical TLC guide features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced. Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures. How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.

Practice of Thin Layer Chromatography

Author : Joseph C. Touchstone
Publisher :
ISBN 13 :
Total Pages : 430 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Practice of Thin Layer Chromatography by : Joseph C. Touchstone

Download or read book Practice of Thin Layer Chromatography written by Joseph C. Touchstone and published by . This book was released on 1983-03 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: A practical how-to guide to all the basic techniques needed to practice thin layer chromatography in biochemical/pharmaceutical research and quality control. This updated edition presents the most current techniques as well as the hows and whys of TLC. Provides step-by-step methods for performing the separations as well as doing related tasks, such as applying the sample, selecting the mobile phase, and quantitation. Includes a special chapter on how to select solvents for the development of a chromatogram to separate specific individual components of a mixture.

Handbook of Thin-Layer Chromatography

Author : Joseph Sherma
Publisher : CRC Press
ISBN 13 : 0824748662
Total Pages : 1356 pages
Book Rating : 4.8/5 (247 download)

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Book Synopsis Handbook of Thin-Layer Chromatography by : Joseph Sherma

Download or read book Handbook of Thin-Layer Chromatography written by Joseph Sherma and published by CRC Press. This book was released on 2003-04-18 with total page 1356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.

Practice of Thin Layer Chromatography

Author : Joseph C. Touchstone
Publisher : John Wiley & Sons
ISBN 13 : 9780471612223
Total Pages : 406 pages
Book Rating : 4.6/5 (122 download)

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Book Synopsis Practice of Thin Layer Chromatography by : Joseph C. Touchstone

Download or read book Practice of Thin Layer Chromatography written by Joseph C. Touchstone and published by John Wiley & Sons. This book was released on 1992-08-04 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Third Edition provides all the basic applications needed to practice thin layer chromatography (TLC). New material includes: the latest techniques on sample preparation and zone detection, the hybridization of TLC with high performance liquid chromatography (HPLC) as it's been developing in the last few years, emphasis on numerous applications of HPTLC involving pharmaceuticals and drugs, plus the fundamental studies of mechanisms, theories and the optimization of TLC.

Instrumental Thin-Layer Chromatography

Author : Colin Poole
Publisher : Elsevier
ISBN 13 : 0323983014
Total Pages : 692 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Instrumental Thin-Layer Chromatography by : Colin Poole

Download or read book Instrumental Thin-Layer Chromatography written by Colin Poole and published by Elsevier. This book was released on 2023-01-14 with total page 692 pages. Available in PDF, EPUB and Kindle. Book excerpt: Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques. This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography. Features individual chapters written by recognized authoritative and visionary experts in the field Provides an overview and focused treatment of a single topic Provides tables and diagrams with commonly used data to facilitate practical work, comparison of results, and decision-making Places modern developments in the research literature into a general context not always apparent to inexperienced users of the technique Offers comprehensive updates to all chapters Includes new chapters on instrument platforms, effects-directed detection, data analysis tools, small-scale and microfluidic planar separation systems, and applications to the separation of amino acids and peptides, the analysis of saccharides and lipids, and forensic analysis

Thin Layer Chromatography in Phytochemistry

Author : Monika Waksmundzka-Hajnos
Publisher : CRC Press
ISBN 13 : 9781420046786
Total Pages : 896 pages
Book Rating : 4.0/5 (467 download)

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Book Synopsis Thin Layer Chromatography in Phytochemistry by : Monika Waksmundzka-Hajnos

Download or read book Thin Layer Chromatography in Phytochemistry written by Monika Waksmundzka-Hajnos and published by CRC Press. This book was released on 2008-03-04 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.

Thin Layer Chromatography in Chiral Separations and Analysis

Author : Teresa Kowalska
Publisher : CRC Press
ISBN 13 : 0849343968
Total Pages : 437 pages
Book Rating : 4.8/5 (493 download)

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Book Synopsis Thin Layer Chromatography in Chiral Separations and Analysis by : Teresa Kowalska

Download or read book Thin Layer Chromatography in Chiral Separations and Analysis written by Teresa Kowalska and published by CRC Press. This book was released on 2007-06-22 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thin layer chromatography (TLC) is well suited for performing enantioseparations for research as well as larger-scale applications. A fast, inexpensive, and versatile separation technique, there are many practical considerations that contribute to its effectiveness. Thin Layer Chromatography in Chiral Separations and Analysis is the first bo

Thin-layer Chromatography

Author : Bernard Fried
Publisher : Marcel Dekker
ISBN 13 :
Total Pages : 468 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Thin-layer Chromatography by : Bernard Fried

Download or read book Thin-layer Chromatography written by Bernard Fried and published by Marcel Dekker. This book was released on 1994 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: This title discusses all phases of thin-layer chromatography (TLC), including the preparation of biological samples prior to TLC, general practices of TLC, and applications based on compound types. Stresses practical rather than theoretical aspects of TLC, compares modern TLC with other separation methods, contains extensive coverage of sample preparation methods, surveys the latest instrumentation for high-performance and overpressured TLC, covers current principles and techniques, and more.

Thin Layer Chromatography in Drug Analysis

Author : Lukasz Komsta
Publisher : CRC Press
ISBN 13 : 1466507152
Total Pages : 1070 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis Thin Layer Chromatography in Drug Analysis by : Lukasz Komsta

Download or read book Thin Layer Chromatography in Drug Analysis written by Lukasz Komsta and published by CRC Press. This book was released on 2013-12-20 with total page 1070 pages. Available in PDF, EPUB and Kindle. Book excerpt: Used routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation. The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separation and analysis of chiral substances. The text addresses the newest advances in TLC instrumentation, two-dimensional TLC, quantification by slit scanning densitometry and image analysis, statistical processing of data, and various detection and identification methods. It also describes the use of TLC for solving a key issue in the drug market—the presence of substandard and counterfeit pharmaceutical products. Part II provides an in-depth overview of a wide range of TLC applications for separation and analysis of particular drug groups. Each chapter contains an introduction about the structures and medicinal actions of the described substances and a literature review of their TLC analysis. A useful resource for chromatographers, pharmacists, analytical chemists, students, and R&D, clinical, and forensic laboratories, this book can be utilized as a manual, reference, and teaching source.

Plant Drug Analysis

Author : Sabine Bladt
Publisher : Springer Science & Business Media
ISBN 13 : 3662023989
Total Pages : 314 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Plant Drug Analysis by : Sabine Bladt

Download or read book Plant Drug Analysis written by Sabine Bladt and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Drug Analysis has proven an invaluable and unique aid for all those involved with drug production and analysis, including pharmacists, chemical and pharmaceutical researchers and technicians, drug importers and exporters, governmental chemical control agencies, and health authorities. From the reviews of the German Edition: "The reviewer would like to recommend this excellent book to all chromatographers, as he considers it highly relevant to the solution of numerous problems. Its main purpose is the demonstration of thin-layer chromatograms of the usual commercial drugs as an aid in testing for identity and purity. ... 165 colour plates, each showing 6 chromatograms and all of superb quality photographs ..." (Journal of Chromatography)

Thin-Layer Chromatography with Flame Ionization Detection

Author : M. Ranny
Publisher : Springer Science & Business Media
ISBN 13 : 9400937059
Total Pages : 197 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Thin-Layer Chromatography with Flame Ionization Detection by : M. Ranny

Download or read book Thin-Layer Chromatography with Flame Ionization Detection written by M. Ranny and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thin-layer chromatography (TLC) has become a common and much favoured separation technique in laboratories in widely varied fields in recent years. Much of the credit for the introduction of this technique into analytical practice at the l 2 end of the 1950s is due to E. Stahl • • This method is simple and is characterized by high separation ability and sufficient sensitivity3; however, some analysts feel that it has passed the peak in its development and will gradually be replaced by the more modem high-performance liquid chromatography (HPLC). This is undoubtedly a very important analytical technique utilizing the specific separa tion properties of a large number of sorbents and the possibility of regulating 4 the flow-rate of the mobile phase by adjusting the pressure • Standardization of the experimental conditions is simpler in HPLC than in TLC, where the activity of the sorbent and flow-rate of the eitlent in the thin layer depend markedly on the relative humidity of the laboratory atmosphere and on the composition of the gaseous phase in the elution chamber. In addition, systems for quantitative detection of the separated ~ones are better developed for HPLC than for classical TLC, where, until recently, cumbersome and often even insufficiently reproducible chemical or gravimetric analysis of the extracts of scraped-off spots or densitometry of the separated zones, located first by pyrolysis or reaction s with suitable detection agents, were the predominant determination methods .

High-Performance Thin-Layer Chromatography (HPTLC)

Author : ManMohan Srivastava
Publisher : Springer Science & Business Media
ISBN 13 : 3642140254
Total Pages : 397 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis High-Performance Thin-Layer Chromatography (HPTLC) by : ManMohan Srivastava

Download or read book High-Performance Thin-Layer Chromatography (HPTLC) written by ManMohan Srivastava and published by Springer Science & Business Media. This book was released on 2010-11-15 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present edited book is the presentation of 18 in-depth national and international contributions from eminent professors, scientists and instrumental chemists from educational institutes, research organizations and industries providing their views on their experience, handling, observation and research outputs on HPTLC, a multi-dimensional instrumentation. The book describes the recent advancements made on TLC which have revolutionized and transformed it into a modern instrumental technique HPTLC. The book addresses different chapters on HPTLC fundamentals: principle, theory, understanding; instrumentation: implementation, optimization, validation, automation and qualitative and quantitative analysis; applications: phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis and potential for hyphenation: HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser. The chapters in the book have been designed in such away that the reader follows each step of the HPTLC in logical order.