Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Download Practical Competitive Binding Assay Methods full books in PDF, epub, and Kindle. Read online Practical Competitive Binding Assay Methods ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author : John Paul Ransom
Publisher :
ISBN 13 :
Total Pages : 152 pages
Book Rating : 4.3/5 (243 download)
Download or read book Practical Competitive Binding Assay Methods written by John Paul Ransom and published by . This book was released on 1976 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Steven J. Mather
Publisher : Springer Science & Business Media
ISBN 13 : 9400917686
Total Pages : 248 pages
Book Rating : 4.4/5 (9 download)
Download or read book Current Directions in Radiopharmaceutical Research and Development written by Steven J. Mather and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Radiophannaceutical research has recently undergone a major change in direction. In past years it has been concerned mainly with the development of perfusion tracers, the biodistribution of which reflect the regional blood flow to areas of major organs such as the heart and brain. However, a major new direction of interest now lies in the development of receptor-binding radio-tracers which can be used to perform in-vivo characterisation of diseased tissues and it is likely that much of the future research in this field will follow this direction. The difficulties in developing such tracers are considerable. The researcher must first identify a promising target for radiopharmaceutical development. High specific activity radioactive molecules must be designed and synthesised which will both bind to the target receptor with high affinity, and also have the physicochemical characteristics which will allow them to reach the target site in sufficient quantity while at the same time showing minimal uptake in non-target tissues. Thus the knowledge base required for radiophannaceutical development has now expanded beyond the limits of radiopharmaceutical chemistry to include aspects of biochemistry, molecular biology and conventional drug design. The portfolio of basic knowledge required to support current radiopharmaceutical development is changing and scientists working in this arena need to be trained in this regard. At the same time, the very latest developments in the field need to be communicated to the scientific community in order to stimulate the advancement of this exciting new direction of research.
Author : Harvey Motulsky
Publisher : Oxford University Press
ISBN 13 : 9780198038344
Total Pages : 352 pages
Book Rating : 4.0/5 (383 download)
Download or read book Fitting Models to Biological Data Using Linear and Nonlinear Regression written by Harvey Motulsky and published by Oxford University Press. This book was released on 2004-05-27 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most biologists use nonlinear regression more than any other statistical technique, but there are very few places to learn about curve-fitting. This book, by the author of the very successful Intuitive Biostatistics, addresses this relatively focused need of an extraordinarily broad range of scientists.
Author : Haian Fu
Publisher : Cambridge University Press
ISBN 13 : 1107377587
Total Pages : 359 pages
Book Rating : 4.1/5 (73 download)
Download or read book Chemical Genomics written by Haian Fu and published by Cambridge University Press. This book was released on 2012-02-13 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in chemistry, biology and genomics coupled with laboratory automation and computational technologies have led to the rapid emergence of the multidisciplinary field of chemical genomics. This edited text, with contributions from experts in the field, discusses the new techniques and applications that help further the study of chemical genomics. The beginning chapters provide an overview of the basic principles of chemical biology and chemical genomics. This is followed by a technical section that describes the sources of small-molecule chemicals; the basics of high-throughput screening technologies; and various bioassays for biochemical-, cellular- and organism-based screens. The final chapters connect the chemical genomics field with personalized medicine and the druggable genome for future discovery of new therapeutics. This book will be valuable to researchers, professionals and graduate students in many fields, including biology, biomedicine and chemistry.
Author : Ge Wu
Publisher : John Wiley & Sons
ISBN 13 : 0470583118
Total Pages : 452 pages
Book Rating : 4.4/5 (75 download)
Download or read book Assay Development written by Ge Wu and published by John Wiley & Sons. This book was released on 2010-06-25 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Essential principles and practice of assay development The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss: The basics of assay development, including foundational concepts and applications Commonly used instrumental methods for both biochemical assays and cell-based assays Assay strategies for protein binding and enzymatic activity Cell-based assays High-throughput screening An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.
Author : Fan Xia
Publisher : Springer
ISBN 13 : 9811078351
Total Pages : 220 pages
Book Rating : 4.8/5 (11 download)
Download or read book Biosensors Based on Sandwich Assays written by Fan Xia and published by Springer. This book was released on 2018-01-09 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.
Author : Atta-ur-Rahman
Publisher : CRC Press
ISBN 13 : 1135287228
Total Pages : 214 pages
Book Rating : 4.1/5 (352 download)
Download or read book Bioassay Techniques for Drug Development written by Atta-ur-Rahman and published by CRC Press. This book was released on 2001-10-04 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of an activity-directed isolation process is to isolate bioactive compounds which may provide structural leads of therapeutic importance. Whereas the traditional process of drug development is long and expensive, simple and rapid bioassays can serve as the starting point for drug discovery. This book presents a range of "bench top" bioassa
Author : Mark A. Williams
Publisher : Humana
ISBN 13 : 9781493958733
Total Pages : 0 pages
Book Rating : 4.9/5 (587 download)
Download or read book Protein-Ligand Interactions written by Mark A. Williams and published by Humana. This book was released on 2016-11-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.
Author : Christopher P. Price
Publisher : Springer
ISBN 13 : 1349112348
Total Pages : 665 pages
Book Rating : 4.3/5 (491 download)
Download or read book Principles and Practice of Immunoassay written by Christopher P. Price and published by Springer. This book was released on 1991-06-18 with total page 665 pages. Available in PDF, EPUB and Kindle. Book excerpt: Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.
Author : Michael G. Tovey
Publisher : John Wiley & Sons
ISBN 13 : 1118075676
Total Pages : 316 pages
Book Rating : 4.1/5 (18 download)
Download or read book Detection and Quantification of Antibodies to Biopharmaceuticals written by Michael G. Tovey and published by John Wiley & Sons. This book was released on 2011-07-12 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive book on the neutralization of recombinant biopharmaceuticals Recombinant biopharmaceuticals are an important tool for treating a range of illnesses; however, their efficacy can be severely impaired by their immunogenicity. When introduced into the body, these pharmaceuticals can cause the immune system to produce anti-drug antibodies (ADAs) that neutralize their effects. The first and only book to cover neutralization in connection with biopharmaceuticals and the measurement and application of neutralizing antibodies in modern medicine at any real length, Detection and Quantification of Antibodies to Biopharmaceuticals: Practical and Applied Considerations offers a comprehensive and in-depth look at all the principal aspects of the detection and quantification of antibodies that are essential to understanding and responding to the challenges they present. Bringing together a large-scale review of neutralization and biopharmaceuticals and the ability to measure, detect, and apply antibodies to modern science and medicine with international regulatory perspectives, the expectations of regulatory authorities, and the strengths and weaknesses of various assays, the book describes several novel ideas for detecting ADAs. Designed to serve as a resource for biopharmaceutical drug development, the book provides biotechnology companies and pharmaceutical drug development specialists, as well as non-experts, with key insights into the design, optimization, and qualification of assays, the establishment of sampling strategies, the choice of appropriate assay end-points, and data analysis for the detection and quantification of neutralizing antibodies.
Author : Terrence P. Kenakin
Publisher : Lippincott Williams & Wilkins
ISBN 13 : 9780397518159
Total Pages : 491 pages
Book Rating : 4.5/5 (181 download)
Download or read book Pharmacologic Analysis of Drug-receptor Interaction written by Terrence P. Kenakin and published by Lippincott Williams & Wilkins. This book was released on 1997 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: The thoroughly revised, updated Third Edition of this highly acclaimed text reflects the past four years' groundbreaking developments in receptor pharmacology, particularly the use of human recombinant receptor systems in drug design and development. Whereas prior editions focused on the correspondence of animal to human receptor systems, this new edition examines drug effects on human receptors obtained by recombinant technology, as well as on physiological natural receptor systems in animals. Two new chapters explain how to produce and use human recombinant receptor systems and discuss the interpretation of data from human recombinant receptor studies. Other chapters describe the use of receptor studies to measure and characterize the biological actions of drugs.
Author : György Keserü
Publisher : John Wiley & Sons
ISBN 13 : 352733582X
Total Pages : 360 pages
Book Rating : 4.5/5 (273 download)
Download or read book Thermodynamics and Kinetics of Drug Binding written by György Keserü and published by John Wiley & Sons. This book was released on 2015-08-17 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical reference for medicinal and pharmaceutical chemists combines the theoretical background with modern methods as well as applications from recent lead finding and optimization projects. Divided into two parts on the thermodynamics and kinetics of drug-receptor interaction, the text provides the conceptual and methodological basis for characterizing binding mechanisms for drugs and other bioactive molecules. It covers all currently used methods, from experimental approaches, such as ITC or SPR, right up to the latest computational methods. Case studies of real-life lead or drug development projects are also included so readers can apply the methods learned to their own projects. Finally, the benefits of a thorough binding mode analysis for any drug development project are summarized in an outlook chapter written by the editors.
Author : Terrence P. Kenakin
Publisher : Springer Science & Business Media
ISBN 13 : 9783540661245
Total Pages : 482 pages
Book Rating : 4.6/5 (612 download)
Download or read book The Pharmacology of Functional, Biochemical, and Recombinant Receptor Systems written by Terrence P. Kenakin and published by Springer Science & Business Media. This book was released on 2000 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: The technology of drug testing is rapidly advancing. This book brings together world renowned experts in the fields of pharmacology and physiology united with the common theme of describing methods, theoretical concepts and in vitro systems used to study drug receptor mechanisms and the action of drugs on receptors. The first section deals with the physiology and pharmacology of integrated natural systems, the second with the new theoretical ideas used to model receptor systems (i.e. the nature of efficacy, receptor activation) and the third with the new in vitro systems available for the study of receptors and drugs. The latest ideas regarding receptor theory are critically evaluated and presented to the reader.
Author : Mario L. Rocci Jr.
Publisher : Springer
ISBN 13 : 3319548026
Total Pages : 239 pages
Book Rating : 4.3/5 (195 download)
Download or read book Regulated Bioanalysis: Fundamentals and Practice written by Mario L. Rocci Jr. and published by Springer. This book was released on 2017-04-24 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: The editors have engaged leading scientists in the field to participate in the development of this book, which is envisioned as a “one of a kind” contribution to the field. The book is a comprehensive text that puts fundamental bioanalytical science in context with current practice, its challenges and ongoing developments. It expands on existing texts on the subject by covering regulated bioanalysis of both small and large molecule therapeutics from both a scientific and regulatory viewpoint. The content will be useful to a wide spectrum of readers: from those new to bioanalysis; to those developing their experience in the laboratory, or working in one of the many critical supporting roles; to seasoned practitioners looking for a solid source of information on this exciting and important discipline.
Author : Hans Bisswanger
Publisher : John Wiley & Sons
ISBN 13 : 3527820809
Total Pages : 574 pages
Book Rating : 4.5/5 (278 download)
Download or read book Practical Enzymology written by Hans Bisswanger and published by John Wiley & Sons. This book was released on 2019-11-06 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Author :
Publisher : Academic Press
ISBN 13 : 0128054344
Total Pages : 686 pages
Book Rating : 4.1/5 (28 download)
Download or read book Peptide, Protein and Enzyme Design written by and published by Academic Press. This book was released on 2016-08-27 with total page 686 pages. Available in PDF, EPUB and Kindle. Book excerpt: De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction. - Continues the legacy of this premier serial with quality chapters on de novo enzyme design - Represents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field - Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism
Author : Patrick Englebienne
Publisher : CRC Press
ISBN 13 : 9780849300615
Total Pages : 404 pages
Book Rating : 4.3/5 (6 download)
Download or read book Immune and Receptor Assays in Theory and Practice written by Patrick Englebienne and published by CRC Press. This book was released on 1999-12-28 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past ten years, employment of immuno- and receptor- assays has grown dramatically. Now used in the pharmaceutical industry for automated screening programs, in the agro-food industry for on-line processing control and food adulteration detection, and in clinical laboratories, they are fully integrated analytical tools. However, the literature often covers only one type of assay or just one of the many systems available. Immune and Receptor Assays in Theory and Practice gathers and organizes the available information to help you establish the best assay for your application. This composite presents the fundamentals of both techniques and introduces practical examples of equation use, antibody and receptor purification, antigen labeling, immunization, and establishment of antibodies for long-term storage. It contrasts the many different assay designs and addresses market trends as the context for developing immuno-assay goals. In addition, this volume summarizes the biochemical and physical properties involved in antibody- and receptor-ligand interactions and reagent manufacture. This is the first, single-volume synthesis of both immuno- and receptor-assays. With theoretical background and practical examples, Immune and Receptor Assays in Theory and Practice allows you to base your experiment on proven techniques, components, and applications for the most reliable results.
