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Plant Molecular Biology A Laboratory Manual
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Book Synopsis Plant Molecular Biology — A Laboratory Manual by : Melody S. Clark
Download or read book Plant Molecular Biology — A Laboratory Manual written by Melody S. Clark and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Book Synopsis Molecular Biology Techniques by : Heather B. Miller
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Book Synopsis Plant Molecular Biology by : P. G. Jones
Download or read book Plant Molecular Biology written by P. G. Jones and published by . This book was released on 1997-10-09 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors describe the most important currently used protocols in plant molecular biology. They give useful tips on stopping points and troubleshooting as well as providing information on safety. Lists of suppliers are included.
Book Synopsis Introductory Plant Biology by : Kingsley R. Stern
Download or read book Introductory Plant Biology written by Kingsley R. Stern and published by . This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods in Plant Molecular Biology by : Mary A. Schuler
Download or read book Methods in Plant Molecular Biology written by Mary A. Schuler and published by Academic Press. This book was released on 2012-12-02 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. - Eight basic experiments which can be used collectively or individually cover - Recombinant Cloning and Screening in E. coli; DNA Sequencing - Plant RNA Isolation and in Vitro Translations - Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations - Experiments that have been student tested for three years - Blueprints for setting up gel rigs - Comprehensive course schedule outlining individual procedures to be finished in each lab segment - Course can be tailored to suit the needs of the individual instructor
Book Synopsis Plant molecular biology: a laboratory manual by : M. Clark
Download or read book Plant molecular biology: a laboratory manual written by M. Clark and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advanced Methods in Molecular Biology and Biotechnology by : Khalid Z. Masoodi
Download or read book Advanced Methods in Molecular Biology and Biotechnology written by Khalid Z. Masoodi and published by Academic Press. This book was released on 2020-10-28 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Download or read book Phage Display written by Carlos F. Barbas and published by CSHL Press. This book was released on 2001 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
Book Synopsis Basic Techniques in Molecular Biology by : Stefan Surzycki
Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Book Synopsis Laboratory Manual of Microbiology, Biochemistry and Molecular Biology by : J. Saxena
Download or read book Laboratory Manual of Microbiology, Biochemistry and Molecular Biology written by J. Saxena and published by Scientific Publishers. This book was released on 2015-05-01 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Though many practical books are available in the market but this Laboratory Manual of Microbiology, Biochemistry and Molecular Biology is an unique combination of protocols that covers maximum (about 80%) of the practicals of various Indian universities for UG and PG courses in Bioscience, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering.
Book Synopsis Botany: A Lab Manual by : Stacy Pfluger
Download or read book Botany: A Lab Manual written by Stacy Pfluger and published by Jones & Bartlett Publishers. This book was released on 2012-12 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis General Botany Laboratory Manual by : Jerry G. Chmielewski
Download or read book General Botany Laboratory Manual written by Jerry G. Chmielewski and published by AuthorHouse. This book was released on 2013-01-21 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: The laboratory component of General Botany provides you the opportunity to view interrelationships between and among structures, to handle live or preserved material, to become familiar with the many terms we use throughout the course, and to learn how to use a microscope properly. Each of you will have your own microscope every week, no exceptions. This laboratory is fundamental, yet integral to your understanding of General Botany. The images in your manual are intended to serve as a guide while you view permanent or prepared slides. These must be viewed by each of you independently. At no time will questions be answered re where is a particular structure, etc., unless the slide is on the stage of your microscope and in focus.The content of the laboratory is rich, as is the terminology. You must come to lab prepared. You must come to lab knowing what the various terms you are about to deal with mean. There is no such thing as finishing early that simply isn't possible.In some laboratory exercises you will be asked to identify structures of an organism. For example, Examine slide 9 labeled Rhizopus sporangia w.m. and identify the mitosporangia, mitospores, columella, mitosporangiophore, and zygotes. In all likelihood you will only be able to see mitosporangia, mitospores, columella, and mitosporangiophores. If zygotes are absent in your slide you note that the population of hyphae you are examining are only reproducing asexually. These questions are written in this manner to further fortify your understanding of the organisms in question and not to trick you. Thinking about what you are viewing is not an option but a necessity!The phylogeny we have adopted in this course is a composite. No single phylogeny best reflects our collective understanding of all the organisms included in this course so we have created one that reflects modern thought and is based on both morphological and molecular data. None is any more correct or incorrect than is any other, but this is the one that we will use, and the one we deem as most acceptable.Rest assured, much still needs to be learned about the evolution of many of the groups we will study. Regardless, the course does provide you a general overview of the evolutionary biology of these various groups. This is your starting point, it is not the endpoint!
Download or read book Antibodies written by Edward Harlow and published by CSHL Press. This book was released on 1988 with total page 748 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.
Download or read book Botany written by Mauseth and published by Jones & Bartlett Publishers. This book was released on 2016-07-06 with total page 829 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Sixth Edition of Botany: An Introduction to Plant Biology provides a modern and comprehensive overview of the fundamentals of botany while retaining the important focus of natural selection, analysis of botanical phenomena, and diversity.
Download or read book CRISPR-Cas written by Jennifer A. Doudna and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.
Book Synopsis Early Development of Xenopus Laevis by : Hazel L. Sive
Download or read book Early Development of Xenopus Laevis written by Hazel L. Sive and published by CSHL Press. This book was released on 2000 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.
Book Synopsis Plant Molecular Biology by : Charles H. Shaw
Download or read book Plant Molecular Biology written by Charles H. Shaw and published by . This book was released on 1988 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant-specific protocols written in a clear and straightforward manner. Details modifications required before standard molecular biology techniques can be applied to plant material. Annotation copyrighted by Book News, Inc., Portland, OR
