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Partitioning In Aqueous Two Phase Systems
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Book Synopsis Partitioning In Aqueous Two – Phase System by : Harry Walter
Download or read book Partitioning In Aqueous Two – Phase System written by Harry Walter and published by Elsevier. This book was released on 2012-12-02 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Book Synopsis Aqueous Two-Phase Systems by : Rajni Hatti-Kaul
Download or read book Aqueous Two-Phase Systems written by Rajni Hatti-Kaul and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
Book Synopsis Aqueous Two-Phase Partitioning by : Boris Y. Zaslavsky
Download or read book Aqueous Two-Phase Partitioning written by Boris Y. Zaslavsky and published by CRC Press. This book was released on 1994-11-15 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.
Book Synopsis Aqueous Two-Phase Systems by : Harry Walter
Download or read book Aqueous Two-Phase Systems written by Harry Walter and published by Elsevier. This book was released on 1994-04-18 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
Book Synopsis Protein Structure Analysis by : Roza Maria Kamp
Download or read book Protein Structure Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.
Book Synopsis Extractive Bioconversions by : B. Mattiasson
Download or read book Extractive Bioconversions written by B. Mattiasson and published by CRC Press. This book was released on 1990-11-30 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contributors from European and US universities and corporations review recent developments in the integration of downstream processing and bioconversion, describing their experience with many separation technologies, including some still in the experimental stage. The topics include the construction
Book Synopsis Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products by : Marco Rito-Palomares
Download or read book Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products written by Marco Rito-Palomares and published by Springer. This book was released on 2017-07-18 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.
Book Synopsis Three Phase Partitioning by : Munishwar Nath Gupta
Download or read book Three Phase Partitioning written by Munishwar Nath Gupta and published by Elsevier. This book was released on 2021-08-11 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more. - Written by researchers who have been pioneers in developing and utilizing three phase partitioning - Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples - Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols
Book Synopsis Liquid Biphasic System by : Pau Loke Show
Download or read book Liquid Biphasic System written by Pau Loke Show and published by Elsevier. This book was released on 2020-11-28 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Downstream bioprocesses have a significant role to play in the creation of a sustainable bio-based economy, enabling the creation of new products and systems from the more sustainable bioprocessing of natural products. Liquid Biphasic System: Fundamentals, Methods, and Applications in Bioseparation Technology explores in detail the fundamental processes and applications of this new separation system, aiding understanding of the basic principles of the technique and offering constructive criticisms on the latest findings.Including coverage of the background, principles, mechanisms, and applications, Liquid Biphasic System addresses how to adapt the technology for the purification of useful compounds with greater cost efficiency and greener processing. It is essential reading for bioprocess engineers, biochemical engineers, biosystem engineers, chemists and microbiologists working in the fields of bioprocessing. Researchers, scientists, and engineers concerned with the selection and evaluation of alternative bioseparation processes will find the book particularly useful. - Provides information and examples of advanced separations in a single source - Includes detailed descriptions of novel bioseparation systems - Covers the latest technologies related to advanced liquid–liquid separation and their applications in various industries
Book Synopsis Ionic-Liquid-Based Aqueous Biphasic Systems by : Mara G. Freire
Download or read book Ionic-Liquid-Based Aqueous Biphasic Systems written by Mara G. Freire and published by Springer. This book was released on 2016-10-05 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.
Book Synopsis Prebiotic Chemistry by : Peter Walde
Download or read book Prebiotic Chemistry written by Peter Walde and published by Springer Science & Business Media. This book was released on 2005-10-13 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mineral Nutrition of Higher Plants by : Horst Marschner
Download or read book Mineral Nutrition of Higher Plants written by Horst Marschner and published by Gulf Professional Publishing. This book was released on 1995 with total page 920 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text presents the principles of mineral nutrition in the light of current advances. For this second edition more emphasis has been placed on root water relations and functions of micronutrients as well as external and internal factors on root growth and the root-soil interface.
Book Synopsis Protein Purification by : Jan-Christer Janson
Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Book Synopsis Applications of Solid Phase Microextraction by : Janusz Pawliszyn
Download or read book Applications of Solid Phase Microextraction written by Janusz Pawliszyn and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 676 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid Phase Microextraction (SPME) has been introduced as a modern alternative to current sample preparation technology, and has a wide range of applications. Focusing on quantitative aspects of analysis, Applications of Solid Phase Microextraction aims to describe these applications. In industry, practical uses of SPME can be found in environmental, food, pharmaceutical, clinical and forensic applications, all of which are described in this book. Important scientific applications such as reaction monitoring, characterization of coatings and distributions of analytes in natural multiphase systems are also discussed. Throughout there are descriptions of new technologies, including new coatings and interfaces for analytical instrumentation (SPME/LC and SPME/CE), automation and calibration processes. Written by internationally recognised experts, edited by the scientist involved in the research since its infancy, and encompassing a wide range of applications, this book will be ideal for anyone wishing to explore the feasibility of using SPME technology.
Book Synopsis Handbook of Bioseparations by : Satinder Ahuja
Download or read book Handbook of Bioseparations written by Satinder Ahuja and published by Elsevier. This book was released on 2000-06-23 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories.Key Features* Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies* Encompasses both analytical and process-scale methods* Elucidates the importance of engineering process control* Details selection of plant and process equipment* Addresses economic considerations* Discusses future developments
Book Synopsis Advanced Separation Techniques for Nuclear Fuel Reprocessing and Radioactive Waste Treatment by : Kenneth L Nash
Download or read book Advanced Separation Techniques for Nuclear Fuel Reprocessing and Radioactive Waste Treatment written by Kenneth L Nash and published by Elsevier. This book was released on 2011-03-15 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced separations technology is key to closing the nuclear fuel cycle and relieving future generations from the burden of radioactive waste produced by the nuclear power industry. Nuclear fuel reprocessing techniques not only allow for recycling of useful fuel components for further power generation, but by also separating out the actinides, lanthanides and other fission products produced by the nuclear reaction, the residual radioactive waste can be minimised. Indeed, the future of the industry relies on the advancement of separation and transmutation technology to ensure environmental protection, criticality-safety and non-proliferation (i.e., security) of radioactive materials by reducing their long-term radiological hazard.Advanced separation techniques for nuclear fuel reprocessing and radioactive waste treatment provides a comprehensive and timely reference on nuclear fuel reprocessing and radioactive waste treatment. Part one covers the fundamental chemistry, engineering and safety of radioactive materials separations processes in the nuclear fuel cycle, including coverage of advanced aqueous separations engineering, as well as on-line monitoring for process control and safeguards technology. Part two critically reviews the development and application of separation and extraction processes for nuclear fuel reprocessing and radioactive waste treatment. The section includes discussions of advanced PUREX processes, the UREX+ concept, fission product separations, and combined systems for simultaneous radionuclide extraction. Part three details emerging and innovative treatment techniques, initially reviewing pyrochemical processes and engineering, highly selective compounds for solvent extraction, and developments in partitioning and transmutation processes that aim to close the nuclear fuel cycle. The book concludes with other advanced techniques such as solid phase extraction, supercritical fluid and ionic liquid extraction, and biological treatment processes.With its distinguished international team of contributors, Advanced separation techniques for nuclear fuel reprocessing and radioactive waste treatment is a standard reference for all nuclear waste management and nuclear safety professionals, radiochemists, academics and researchers in this field. - A comprehensive and timely reference on nuclear fuel reprocessing and radioactive waste treatment - Details emerging and innovative treatment techniques, reviewing pyrochemical processes and engineering, as well as highly selective compounds for solvent extraction - Discusses the development and application of separation and extraction processes for nuclear fuel reprocessing and radioactive waste treatment
Book Synopsis An Introduction to Reservoir Simulation Using MATLAB/GNU Octave by : Knut-Andreas Lie
Download or read book An Introduction to Reservoir Simulation Using MATLAB/GNU Octave written by Knut-Andreas Lie and published by Cambridge University Press. This book was released on 2019-08-08 with total page 677 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents numerical methods for reservoir simulation, with efficient implementation and examples using widely-used online open-source code, for researchers, professionals and advanced students. This title is also available as Open Access on Cambridge Core.
