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Author : David M. Black
Publisher :
ISBN 13 :
Total Pages : 364 pages
Book Rating : 4.:/5 (71 download)
Download or read book Novel Methods for Characterization of Intact Proteins by Mass Spectrometry written by David M. Black and published by . This book was released on 2005 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : William S. Hancock
Publisher : CRC Press
ISBN 13 : 9780849378225
Total Pages : 280 pages
Book Rating : 4.3/5 (782 download)
Download or read book New Methods in Peptide Mapping for the Characterization of Proteins written by William S. Hancock and published by CRC Press. This book was released on 1995-10-23 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.
Author : Bifan Chen
Publisher :
ISBN 13 :
Total Pages : 215 pages
Book Rating : 4.:/5 (123 download)
Download or read book Advancing Intact Protein Analysis by Top-down Mass Spectrometry written by Bifan Chen and published by . This book was released on 2019 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of proteins is critical for understanding cellular functions at the molecular level. Top-down mass spectrometry (MS) has emerged as a premier tool for global and comprehensive analysis of proteoforms. The top-down approach retains intact mass information, providing a "bird's-eye" view of the proteome and allowing for identification of novel proteoforms, in-depth sequence characterization, and quantification of disease associated post-translational modifications (PTMs). However, many technical challenges still exist. The research described here involves analytical development in top-down MS, particularly in the areas of enrichment, separation, and characterization of samples ranging from standard proteins and complex lysates, to large therapeutic biomolecules. Chapter 1 provides an introduction and review of recent advances in different aspects of top-down proteomics. Chapters 2 and 3 are related to the study of intact phosphoproteins. Specifically, chapter 2 describes the use of functionalized nanoparticles for enrichment and the subsequent coupling of online liquid chromatography (LC)-MS for characterizing endogenous phosphoproteins from complex cell lysates. Chapter 3 investigates how phosphorylation moieties might influence the efficiency of electron capture dissociation (ECD). Chapters 4 and 5 focus on the development of hydrophobic interaction chromatography (HIC) that could be coupled online directly with MS and its applications to therapeutic molecules (monoclonal antibodies). Chapter 6 describes a middle-down approach to obtain multi-attribute of both cysteine and lysine conjugated antibody-drug conjugates, which overcomes some current challenges using HIC-MS and the top-down approach. Overall, these analytical developments expand the toolbox of the top-down approach and generally facilitate the analysis of intact proteins.
Author : Mike S. Lee
Publisher : John Wiley & Sons
ISBN 13 : 1119359368
Total Pages : 282 pages
Book Rating : 4.1/5 (193 download)
Download or read book Protein Analysis using Mass Spectrometry written by Mike S. Lee and published by John Wiley & Sons. This book was released on 2017-05-26 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents Practical Applications of Mass Spectrometry for Protein Analysis and Covers Their Impact on Accelerating Drug Discovery and Development Covers both qualitative and quantitative aspects of Mass Spectrometry protein analysis in drug discovery Principles, Instrumentation, Technologies topics include MS of peptides, proteins, and ADCs , instrumentation in protein analysis, nanospray technology in MS protein analysis, and automation in MS protein analysis Details emerging areas from drug monitoring to patient care such as Identification and validation of biomarkers for cancer, targeted MS approaches for biomarker validation, biomarker discovery, and regulatory perspectives Brings together the most current advances in the mass spectrometry technology and related method in protein analysis
Author : John R. Chapman
Publisher : Springer Science & Business Media
ISBN 13 : 1592590454
Total Pages : 539 pages
Book Rating : 4.5/5 (925 download)
Download or read book Mass Spectrometry of Proteins and Peptides written by John R. Chapman and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Little more than three years down the line and I am already writing the Preface to a second volume to follow Protein and Peptide Analysis by Mass . What has happened in between these times to make this second venture worthwhile? New types of mass spectrometric instrumentation have appeared so that new techniques have become possible and existing techniques have become much more feasible. More particularly, however, the newer ionization te- niques, introduced for the analysis of high molecular weight materials, have now been thoroughly used and studied. As a result, there has been an en- mous improvement in the associated sample handling technology so that these methods are now routinely applied to much smaller sample amounts as well as to more intractable samples. Again, this particular community of mass spectrometry users has both increased in number and diversified. And, riding this wave of acceptance, leaders in the field have set their sights on more complex problems: molecular interaction, ion structures, quantitation, and kinetics are just a few of the newer areas reported in Mass Spectrometry of Proteins and Peptides. As with the first volume, one purpose of this collection, Mass Spectr- etry of Proteins and Peptides, is to show the reader what can be done by the application of mass spectrometry, and perhaps even to encourage the reader to venture down new paths.
Author : Igor A. Kaltashov
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 3110545063
Total Pages : 369 pages
Book Rating : 4.1/5 (15 download)
Download or read book Mass Spectrometry in Biopharmaceutical Analysis written by Igor A. Kaltashov and published by Walter de Gruyter GmbH & Co KG. This book was released on 2021-11-22 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biopharmaceuticals are a unique class of compounds due to their extreme structural complexity. The current text puts together a variety of the state‐of‐the art approaches that use mass spectrometry to evaluate various aspects of biopharmaceutical products ranging from monitoring stress‐related structural changes to their quantitation in pharmacokinetic studies.
Author : Michael Nshanian
Publisher :
ISBN 13 :
Total Pages : 175 pages
Book Rating : 4.:/5 (15 download)
Download or read book Methodologies and Applications for the Analysis of Intact Proteins and Protein-ligand Interactions by Top-down Mass Spectrometry written by Michael Nshanian and published by . This book was released on 2018 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advent of top-down protein mass spectrometry (MS), or direct analysis of intact proteins forgoing proteolysis, has transformed the field of protein mass spectrometry, ushering in a new era of protein identification and characterization together with a new set of challenges. The analysis of intact proteins and their direct fragmentation in tandem (MS/MS) mode helps overcome the "inference" problem associated with peptide-based bottom-up proteomics; that is, correctly assigning given peptide fragments and their modifications to the intact protein from which they originated. Despite its many advantages, however, the top-down approach requires extensive sample fractionation and suffers from low sensitivity but much progress has been made. From recently-developed cross-linked polyacrylamide gels, from which intact proteins can be more easily recovered, to the discovery of reagents that enhance protein charging in electrospray ionization (ESI), there have been considerable gains in detection and sensitivity, offering the potential for a more complete and accurate characterization of a "proteoform": the full complement of the combinatorial possibilities that could arise from a given gene product. Top-down MS also includes the study of proteins in their native or native-like states. This is especially important in characterizing disease-related proteins, particularly in the context of protein aggregation. Native MS, using electron-capture dissociation (ECD) and ion mobility spectrometry (IMS), enables the study of protein-inhibitor complexes in the gas phase, offering structural insight into stoichiometry, site of inhibitor binding and mechanism of inhibition. In addition, intact analysis and electron-based fragmentation enable the detection of thermally-labile post-translational modifications like phosphorylation, known to play key regulatory roles in shifting proteins towards cytotoxic states. Top-down method developments in protein recovery, separation and supercharging have led to improvements in detection and sensitivity, while top-down MS applications to structural characterization of disease-related proteins have shed more light on the mechanisms of cytotoxic aggregation, offering greater promise of therapeutic development.
Author : Allan Matte
Publisher : Elsevier
ISBN 13 : 0081030193
Total Pages : 222 pages
Book Rating : 4.0/5 (81 download)
Download or read book Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics written by Allan Matte and published by Elsevier. This book was released on 2020-09-07 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Author : Thomas Letzel
Publisher : Royal Society of Chemistry
ISBN 13 : 1849731829
Total Pages : 195 pages
Book Rating : 4.8/5 (497 download)
Download or read book Protein and Peptide Analysis by LC-MS written by Thomas Letzel and published by Royal Society of Chemistry. This book was released on 2011 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.
Author : Arnaud Delobel
Publisher :
ISBN 13 : 9781071612415
Total Pages : 401 pages
Book Rating : 4.6/5 (124 download)
Download or read book Mass Spectrometry of Glycoproteins written by Arnaud Delobel and published by . This book was released on 2021 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents methods used for the analysis of glycoproteins at different levels--intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.
Author : Guodong Chen
Publisher : Springer Science & Business Media
ISBN 13 : 1441978623
Total Pages : 408 pages
Book Rating : 4.4/5 (419 download)
Download or read book Characterization of Protein Therapeutics using Mass Spectrometry written by Guodong Chen and published by Springer Science & Business Media. This book was released on 2014-07-08 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.
Author :
Publisher :
ISBN 13 :
Total Pages : 442 pages
Book Rating : 4.:/5 (811 download)
Download or read book Strategies for Protein and Peptide Characterization and Quantification Using Electron-transfer Dissociation Mass Spectrometry and Intrinsic Fluorescence written by and published by . This book was released on 2012 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: STRATEGIES FOR PROTEIN AND PEPTIDE CHARACTERIZATION AND QUANTIFICATION USING ELECTRON-TRANSFER DISSOCIATION MASS SPECTROMETRY AND INTRINSIC FLUORESCENCE Jason D. Russell Under the supervision of Associate Professor Joshua J. Coon At the University of Wisconsin-Madison The following chapters detail strategies for peptide and protein sequence analysis featuring electron-transfer dissociation (ETD) tandem mass spectrometry (MS/MS) and quantification using ultraviolet light-induced intrinsic fluorescence (UV-IF). Chapter 1 provides a brief background and overview. Chapter 2 discusses the optimization of the ETD MS/MS duty cycle for large-scale shotgun experiments. In Chapter 3, the first comprehensive analysis of peptide anions using negative ETD (NETD) is detailed. I report in Chapter 4 on the performance of an ion-ion reaction cell for intact protein analysis using large precursor populations for ETD MS/MS. The application of UV-IF for peptide detection and quantification using a custom fluorescence excitation and detection device is discussed in Chapter 5. An analysis of intact proteins from the 26S proteasome of Arabidopsis using top-down mass spectrometry and quantification by UV-IF is described in Chapter 6. In coordination with the Wisconsin Initiative for Science Literacy (WISL), Chapter 7 is a general description of my graduate research intended for non-specialists in an effort to promote science literacy in the broader community.
Author : Fanyu Meng
Publisher :
ISBN 13 :
Total Pages : 174 pages
Book Rating : 4.:/5 (551 download)
Download or read book Direct Analysis of Intact Proteins from Microorganisms Using Top Down Mass Spectrometry written by Fanyu Meng and published by . This book was released on 2003 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Dietmar Hammerschmid
Publisher :
ISBN 13 :
Total Pages : 297 pages
Book Rating : 4.:/5 (119 download)
Download or read book Novel Mass Spectrometry Strategies for the Characterization of Membrane-bound and Oxidativelabeled Proteins written by Dietmar Hammerschmid and published by . This book was released on 2019 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ian M. Rosenberg
Publisher : Springer Science & Business Media
ISBN 13 : 1461220564
Total Pages : 541 pages
Book Rating : 4.4/5 (612 download)
Download or read book Protein Analysis and Purification written by Ian M. Rosenberg and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 541 pages. Available in PDF, EPUB and Kindle. Book excerpt: How one goes about analyzing proteins is a constantly evolving field that is no longer solely the domain of the protein biochemist. Investi gators from diverse disciplines find themselves with the unanticipated task of identifying and analyzing a protein and studying its physical properties and biochemical interactions. In most cases, the ultimate goal remains understanding the role(s) that the target protein is playing in cellular physiology. It was my intention that this manual would make the initial steps in the discovery process less time consuming and less intimidating. This book is not meant to be read from cover to cover. The expanded Table of Contents and the index should help locate what you are seeking. My aim was to provide practically oriented information that will assist the experimentalist in benchtop problem solving. The appendices are filled with diverse information gleaned from catalogs, handbooks, and manuals that are presented in a distilled fashion designed to save trips to the library and calls to technical service representatives. The user is encouraged to expand on the tables and charts to fit individual experimental situations. This second edition pays homage to the computer explosion and the various genome projects that have revolutionized how benchtop scientific research is performed. Bioinformatics and In silica science are here to stay. However, the second edition still includes recipes for preparing buffers and methods for lysing cells.
Author : Sean Duncan Dunham
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (136 download)
Download or read book Enhancing the Characterization of Intact Proteins by Ultraviolet Photodissociation Mass Spectrometry written by Sean Duncan Dunham and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Access to high resolution mass spectrometers and high energy modes of activation such as electron- and photon-based modalities have enabled wider adoption of top-down methodologies, or strategies that allow the study of intact proteins. However, interpretation of MS/MS spectra of large proteins remains difficult owing to spectral congestion, charge capacity limitations, and other challenges. In particular, for ultraviolet photodissociation (UVPD) of intact proteins, a single laser pulse is typically used to avoid secondary dissociation of fragment ions that occurs when multiple pulses are employed. Consequently, a large amount of the precursor ion population remains undissociated, meaning a large portion of the potential signal is not effectively utilized. Secondary dissociation results in the generation of less informative small terminal and internal fragment ions. Internal fragments are typically ignored due to the computational challenges associated with accounting for them. The following research focuses on the use of fragment ion protection (FIP) during 193 nm UVPD to counter secondary dissociation when utilizing multiple laser pulses and the exploration of the benefits and pitfalls when considering internal fragment ions generated by 193 nm UVPD. In, summary, FIP increased the center sequence coverage of large proteins, but there is room for improvement. The inclusion of internal fragment ions can aid in enhancing the sequence coverage of intact proteins. However, the majority of internal fragment ions are not reliably identified across multiple replicates, reflecting a high risk of false positive identifications when they are considered. These findings are described in this thesis
Author : Angelina Taichrib
Publisher :
ISBN 13 :
Total Pages : 115 pages
Book Rating : 4.:/5 (828 download)
Download or read book Analysis of Peptides and Proteins by Capillary Electrophoresis-mass Spectrometry written by Angelina Taichrib and published by . This book was released on 2012 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt:
