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Author : E. R. Levy
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (141 download)
Download or read book Non Isotopic Methods in Molecular Biology written by E. R. Levy and published by . This book was released on 1995 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : E. R. Levy
Publisher :
ISBN 13 :
Total Pages : 268 pages
Book Rating : 4.3/5 (91 download)
Download or read book Non-isotopic Methods in Molecular Biology written by E. R. Levy and published by . This book was released on 1995 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Radioactive chemicals, while playing a vital role in many research and clinical techniques for nucleic acid analysis, are considered dangerous to the laboratory worker and require specialist handling and disposal equipment. This book describes how detailed molecular analysis of DNA and RNA can be performed using a range of non-radioactive chemicals giving similar if not better results than the radioactive methods. It will be a valuable book for molecular biologists and pathologists in academic and clinical research and also those working in clinical diagnostic laboratories.
Author : Larry J. Kricka
Publisher : Elsevier
ISBN 13 : 0080537669
Total Pages : 539 pages
Book Rating : 4.0/5 (85 download)
Download or read book Nonisotopic Probing, Blotting, and Sequencing written by Larry J. Kricka and published by Elsevier. This book was released on 1995-06-01 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. - Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules - Gives protocols for nonisotopic DNA sequencing - new in this edition - Gives extensive, practical information - Presents background information for each method - Provides expert accounts from the inventor or developer of each method - Contains seven entirely new chapters - Covers all major types of nonisotopic procedures for labeling and detection
Author : Leonard Davis
Publisher : Elsevier
ISBN 13 : 044460149X
Total Pages : 401 pages
Book Rating : 4.4/5 (446 download)
Download or read book Basic Methods in Molecular Biology written by Leonard Davis and published by Elsevier. This book was released on 2012-12-02 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.
Author : T. Chard
Publisher : Elsevier Science
ISBN 13 : 9780444821195
Total Pages : pages
Book Rating : 4.8/5 (211 download)
Download or read book An Introduction to Radioimmunoassay and Related Techniques written by T. Chard and published by Elsevier Science. This book was released on 1995-11-20 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoassay procedures (isotopic and non-isotopic) have become one of the single most important techniques in present-day diagnostic medicine. This book is designed as an introductory test for the staff of clinical research laboratories who conduct or intend to conduct such techniques, and will be of great value to the clinicians who make use of such services. The volume takes a three-pronged approach in it's in-depth presentation: explanation of the basic principles and applications of radioimmunoassays and non-isotopic immunoassays; practical illustrations of the various steps involved in immunoassays; discussion of the problems and pitfalls in immunoassays and how to avoid them. This fifth revised edition is a worthy successor to it's predecessors in this famous "Laboratory Techniques" series.
Author : Shanmugasundaram Ganapathy-Kanniappan
Publisher : Springer Nature
ISBN 13 : 3030997006
Total Pages : 76 pages
Book Rating : 4.0/5 (39 download)
Download or read book Isotopic Tracer Techniques in Preclinical Research written by Shanmugasundaram Ganapathy-Kanniappan and published by Springer Nature. This book was released on 2022-05-03 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book serves as a practical guide and a manual on the application of isotopic tracers in understanding the cellular processes (e.g. molecular interactions, protein synthesis) in growth, development, and disease conditions. The techniques described in the book have been carefully chosen to underscore the role and relevance of isotopic tracers in the identification of molecular targets and mechanisms, and preclinical validation of potential therapeutics. This book is intended to be a valuable resource to non-experts that may include students, researchers, educators/teachers, and others who have an interest to understand the application of isotopic tracers in research. Each chapter includes a background, an outline of the method and underlying principles, and a detailed step-wise protocol. To augment the clarity and intellectual grasp, the chapters also include relevant illustrations and study questions. More importantly, the book covers techniques involving radioactive as well as non-radioactive tracers to expand the scope of isotopes in preclinical research including basic and applied science.
Author : J.M. Walker
Publisher : Springer Science & Business Media
ISBN 13 : 9401165637
Total Pages : 304 pages
Book Rating : 4.4/5 (11 download)
Download or read book Techniques in Molecular Biology written by J.M. Walker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Author : That T. Ngo
Publisher : Springer Science & Business Media
ISBN 13 : 1468454668
Total Pages : 487 pages
Book Rating : 4.4/5 (684 download)
Download or read book Nonisotopic Immunoassay written by That T. Ngo and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.
Author : Marc G. Dumont
Publisher : Humana
ISBN 13 : 9781493997237
Total Pages : 247 pages
Book Rating : 4.9/5 (972 download)
Download or read book Stable Isotope Probing written by Marc G. Dumont and published by Humana. This book was released on 2020-08-13 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.
Author : Ian A. Darby
Publisher : Humana
ISBN 13 : 9781588294029
Total Pages : 270 pages
Book Rating : 4.2/5 (94 download)
Download or read book In Situ Hybridization Protocols written by Ian A. Darby and published by Humana. This book was released on 2005-12-15 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
Author : Larry J. Kricka
Publisher : Academic Press
ISBN 13 : 0323137717
Total Pages : 373 pages
Book Rating : 4.3/5 (231 download)
Download or read book Nonisotopic DNA Probe Techniques written by Larry J. Kricka and published by Academic Press. This book was released on 2012-12-02 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided.This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection.Gives extensive practical informationCovers major types of nonisotopic labels and proceduresPresents background information for each methodProvides strategies and detailed experimental procedures for labeling and detecting DNA sequences byFluorescenceChemiluminescenceBioluminescenceColorimetry
Author : Maik A Jochmann
Publisher : Royal Society of Chemistry
ISBN 13 : 1782626107
Total Pages : 306 pages
Book Rating : 4.7/5 (826 download)
Download or read book Compound-specific Stable Isotope Analysis written by Maik A Jochmann and published by Royal Society of Chemistry. This book was released on 2015-11-09 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of Compound-specific Stable Isotope Analysis (CSIA) is increasing in many areas of science and technology for source allocation, authentication, and characterization of transformation reactions. Until now, there have been no textbooks available for students with an analytical chemical background or basic introductory books emphasising the instrumentation and theory. This book is the first to focus solely on stable isotope analysis of individual compounds in sometimes complex mixtures. It acts as both a lecture companion for students and a consultant for advanced scientists in fields including forensic and environmental science. The book starts with a brief history of the field before going on to explain stable isotopes from scratch. The different ways to express isotope abundances are introduced together with isotope effects and isotopic fractionation. A detailed account of the required technical equipment and general procedures for CSIA is provided. This includes sections on derivatization and the use of microextraction techniques in GC-IRMS. The very important topic of referencing and calibration in CSIA is clearly described. This differs from approaches used in quantitative analysis and is often difficult for the newcomer to comprehend. Examples of successful applications of CSIA in food authenticity, forensics, archaeology, doping control, environmental science, and extraterrestrial materials are included. Applications in isotope data treatment and presentation are also discussed and emphasis is placed on the general conclusions that can be drawn from the uses of CSIA. Further instrumental developments in the field are highlighted and selected experiments are introduced that may act as a basis for a short practical course at graduate level.
Author : John Crocker
Publisher : John Wiley & Sons
ISBN 13 : 9780470844755
Total Pages : 428 pages
Book Rating : 4.8/5 (447 download)
Download or read book Molecular Biology in Cellular Pathology written by John Crocker and published by John Wiley & Sons. This book was released on 2003-06-02 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.
Author : Rob Ellam
Publisher : Oxford University Press
ISBN 13 : 0198723628
Total Pages : 153 pages
Book Rating : 4.1/5 (987 download)
Download or read book Isotopes written by Rob Ellam and published by Oxford University Press. This book was released on 2016 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isotopes are used in many areas of science and technology, including medicine, archaeology, and nuclear physics. They are central to our understanding of the Earth's past and current processes. Here, Rob Ellam explains the importance and applications of stable and radioactive isotopes.
Author : Terry Brown
Publisher : OUP Oxford
ISBN 13 : 0191565644
Total Pages : 261 pages
Book Rating : 4.1/5 (915 download)
Download or read book Volume I written by Terry Brown and published by OUP Oxford. This book was released on 2000-10-05 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. Volume 1 concentrates on the procedures for DNA and RNA manipulation: purification, electrophoresis, and the construction and cloning of recombinant molecules. It also includes a general introduction to molecular biology in the laboratory and a survey of cloning vectors for Escherichia Coli.
Author : Steven A. Williams
Publisher : Jones & Bartlett Learning
ISBN 13 : 9780763733292
Total Pages : 272 pages
Book Rating : 4.7/5 (332 download)
Download or read book Laboratory Investigations in Molecular Biology written by Steven A. Williams and published by Jones & Bartlett Learning. This book was released on 2007 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Investigations in Molecular Biology presents well-tested protocols in molecular biology that are commonly used in currently active research labs. It is an ideal laboratory manual for college level courses in molecular biology. Because of the modular organization of the manual, laboratory courses can be assembled that would be ideal for science professionals, graduate students, undergraduate students and even advanced high school students in AP courses. The manual is also intended to be useful as a laboratory "bench reference". The experiments are designed to guide students through realistic research projects and to provide students with instruction in methods and approaches that can be immediately translated into research projects conducted in modern research laboratories. Although these experiments have been conducted and optimized over 20 years of teaching the New England Biolabs Molecular Biology Summer Workshops, they are real research projects, not "canned" experiments. Based on extensive teaching experience using these protocols, the authors have found that conducting these experiments as described in these protocols serves to effectively instruct students and science professions in the basic methods of molecular biology. An additional unique feature is that the protocols described in the manual are accompanied by available reagent kits that provide quality-tested, pre-packaged reagents to ensure the successful application of these protocols in a laboratory course setting.
Author : Sarah L. Rowland-Jones
Publisher :
ISBN 13 : 0199638160
Total Pages : 376 pages
Book Rating : 4.1/5 (996 download)
Download or read book Lymphocytes written by Sarah L. Rowland-Jones and published by . This book was released on 2000 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.
