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Negative Staining Cryoelectron
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Book Synopsis Negative Staining and Cryoelectron Microscopy by : 0 J.R. Harris,
Download or read book Negative Staining and Cryoelectron Microscopy written by 0 J.R. Harris, and published by Taylor & Francis. This book was released on 2023-05-09 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the start of biological studies using the transmission electron microscope scientists have sought to develop procedures for the preparation and investigation of the thinly spread specimens of biological particulates. Negative Staining and Cryoelect
Book Synopsis NEGATIVE STAINING CRYOELECTRON by : J. HARRIS
Download or read book NEGATIVE STAINING CRYOELECTRON written by J. HARRIS and published by Taylor & Francis. This book was released on 1997 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the start of biological studies using the transmission electron microscope scientists have sought to develop procedures for the preparation and investigation of the thinly spread specimens of biological particulates. Negative Staining and Cryoelect
Book Synopsis Negative Staining and Cryoelectron Microscopy by : 0 J.R. Harris
Download or read book Negative Staining and Cryoelectron Microscopy written by 0 J.R. Harris and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the start of biological studies using the transmission electron microscope scientists have sought to develop procedures for the preparation and investigation of the thinly spread specimens of biological particulates. Negative Staining and Cryoelect
Author :M. A. Nasser Hajibagheri Publisher :Springer Science & Business Media ISBN 13 :1592592015 Total Pages :292 pages Book Rating :4.5/5 (925 download)
Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri
Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Book Synopsis Biomolecular and Bioanalytical Techniques by : Vasudevan Ramesh
Download or read book Biomolecular and Bioanalytical Techniques written by Vasudevan Ramesh and published by John Wiley & Sons. This book was released on 2019-06-10 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.
Book Synopsis Single-particle Cryo-electron Microscopy by : Joachim Frank
Download or read book Single-particle Cryo-electron Microscopy written by Joachim Frank and published by World Scientific Publishing Company. This book was released on 2017-12-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as important results obtained by this technique in the study of biological macromolecules and their interactions. Importantly, neither symmetries nor ordered arrangements (as in two-dimensional crystals, helical assemblies, icosahedral viruses) are required. Although the biological applications are mainly in the area of ribosome structure and function, the elucidation of membrane channel structures and their activation and gating mechanisms are represented, as well. The book is introduced by a commentary that explains the original development of concepts, describes the contributions of the author's colleagues and students, and shows how challenges were overcome as the technique matured. Along the way, the ribosome served as an example for a macromolecule with intricate structure and conformational dynamics that pose challenges for three-dimensional visualization. Toward the end of the book -- bringing us to the present time -- molecular structures with near-atomic resolution are presented, and a novel type of computational analysis, manifold embedding, is introduced. Single-particle cryo-EM is currently revolutionizing structural biology, presenting a powerful alternative to X-ray crystallography as a means to solve the structure of biological macromolecules. The book presents in one place a number of articles containing key advances in mathematical and computational methods leading up to the present time. Secondly, the development of the technique over the years is reflected by ever-expanding discoveries in the field of ribosome structure and function. Thirdly, as all histories of ideas, the history of concepts pertaining to this new method of visualization is fascinating all in itself.
Download or read book Negative Staining written by M. A. Hayat and published by McGraw-Hill Companies. This book was released on 1990 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Cryo-negative Staining by : Sacha De Carlo
Download or read book Cryo-negative Staining written by Sacha De Carlo and published by . This book was released on 2002 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book cryoEM written by Tamir Gonen and published by Humana. This book was released on 2021-12-28 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology.
Book Synopsis Three-Dimensional Electron Microscopy of Macromolecular Assemblies by : Frank Joachim
Download or read book Three-Dimensional Electron Microscopy of Macromolecular Assemblies written by Frank Joachim and published by Elsevier. This book was released on 1996-01-24 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized* Describes methods that have been instrumental in exploring the three-dimensional structure of* the nuclear pore complex* the calcium release channel;* the ribosome* chaperonins
Book Synopsis Liquid Cell Electron Microscopy by : Frances M. Ross
Download or read book Liquid Cell Electron Microscopy written by Frances M. Ross and published by Cambridge University Press. This book was released on 2017 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: 2.6.2 Electrodes for Electrochemistry
Book Synopsis Immuno-Gold Electron Microscopy in Virus Diagnosis and Research by : Alexander D. Hyatt
Download or read book Immuno-Gold Electron Microscopy in Virus Diagnosis and Research written by Alexander D. Hyatt and published by CRC Press. This book was released on 1992-11-10 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.
Book Synopsis Diagnostic Electron Microscopy by : John Stirling
Download or read book Diagnostic Electron Microscopy written by John Stirling and published by John Wiley & Sons. This book was released on 2013-01-22 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Electron Microscopy Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.
Book Synopsis Cryo-EM Part A: Sample Preparation and Data Collection by :
Download or read book Cryo-EM Part A: Sample Preparation and Data Collection written by and published by Academic Press. This book was released on 2010-09-30 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide
Download or read book Taxonomy of Prokaryotes written by and published by Academic Press. This book was released on 2011-12-05 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Taxonomy of Prokaryotes, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the microbiologist interested in this specialty, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology. Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting-edge protocols to directly benefit your research.
Book Synopsis Structural Biology in Drug Discovery by : Jean-Paul Renaud
Download or read book Structural Biology in Drug Discovery written by Jean-Paul Renaud and published by John Wiley & Sons. This book was released on 2020-01-09 with total page 1367 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the most comprehensive and up-to-date overview of structure-based drug discovery covering both experimental and computational approaches, Structural Biology in Drug Discovery: Methods, Techniques, and Practices describes principles, methods, applications, and emerging paradigms of structural biology as a tool for more efficient drug development. Coverage includes successful examples, academic and industry insights, novel concepts, and advances in a rapidly evolving field. The combined chapters, by authors writing from the frontlines of structural biology and drug discovery, give readers a valuable reference and resource that: Presents the benefits, limitations, and potentiality of major techniques in the field such as X-ray crystallography, NMR, neutron crystallography, cryo-EM, mass spectrometry and other biophysical techniques, and computational structural biology Includes detailed chapters on druggability, allostery, complementary use of thermodynamic and kinetic information, and powerful approaches such as structural chemogenomics and fragment-based drug design Emphasizes the need for the in-depth biophysical characterization of protein targets as well as of therapeutic proteins, and for a thorough quality assessment of experimental structures Illustrates advances in the field of established therapeutic targets like kinases, serine proteinases, GPCRs, and epigenetic proteins, and of more challenging ones like protein-protein interactions and intrinsically disordered proteins
Book Synopsis Veterinary Virology by : Frank J. Fenner
Download or read book Veterinary Virology written by Frank J. Fenner and published by Academic Press. This book was released on 2014-06-28 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: Veterinary Virology deals with basic biomedical virology and the clinical discipline of infectious diseases. The book discusses the principles of virology as effecting future developments in the search for preventive and management of infectious diseases in animals, whether singly or as a whole herd or flock. Part I explains the principles of animal virology including the structure, composition, classification, nomenclature, cultivation, and assay of viruses. This part also discusses viral genetics, replication, and evolution (including mutation and genetic engineering). The book also reviews the pathogenesis of viruses, host resistance and susceptibility, as well as the mechanisms of persistent infections and tumor induction. Part II deals with viruses found in domestic animals; this part also explains in detail the properties, replication methods, pathogenesis, immunity, diagnosis, and control of some common viruses. The book discusses some other families of viruses of which no members are yet known as to have caused serious or important diseases in animals. Veterinarians, immunologists, virologists, molecular researchers, students, and academicians in the discipline of virology and cellular biology, as well as livestock owners will find this book helpful.
