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Nanoscale Protein Analysis Utilizing Capillary Electrophoresis And Laser Induced Fluorescence Detection
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Book Synopsis Nanoscale Protein Analysis Utilizing Capillary Electrophoresis and Laser-induced Fluorescence Detection by : Michael D. Harvey
Download or read book Nanoscale Protein Analysis Utilizing Capillary Electrophoresis and Laser-induced Fluorescence Detection written by Michael D. Harvey and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The trend towards high throughput applications and miniaturization necessitates approaches capable of microlitre volume sampling and low protein concentration detection. Furthermore, one of the major trends in high throughput screening is the growing replacement of technologies that depend on radioactivity to generate a signal with those that rely on fluorescence. This trend towards non-radioactive detection in general can be understood by some of the advantages inherent to these methods over radioactive modes. These include a significant reduction in safety concerns leading to a relaxation of strict laboratory procedures, elimination of expensive waste disposal, extended shelf-life of labeled reagents, and the possibility of acquiring multiplexed data through the spectral isolation of different wavelength signals. A variety of capillary electrophoretic (CE) approaches utilizing laser-induced fluorescence (LIF) have thus been developed, providing researchers with valuable tools in protein analysis. Various covalent and non-covalent fluorescent derivatization approaches have been investigated, with emphasis on biochemical and/or clinical applications. The non-covalent dye, NanoOrange, is used as a clinical diagnostic tool for early disease diagnosis, quantitating nanomolar concentrations of human serum albumin in solution, and obtaining fluorescence-based biofluid profiles. An alternate non-covalent labeling approach utilizing the fluorescent probe, Sypro Red, and capillary gel electrophoresis allows for rapid, sensitive analysis of protein sample purity as well as molecular weight determination. These two non-covalent approaches are complemented by the development of a fluorescent Insulin-Like Growth Factor-I (IGF-I) analog for use in bioanalytical applications. Specific derivatization reaction conditions were developed to selectively label the N-terminus of the analog hence preserve biological activity. High-performance liquid chromatography and electrospray mass spectrometry were used to confirm the extent of labeling and modification site. Antibody recognition of this fluorescent analog was evaluated using CE-LIF, illustrating the clinical utility of this diagnostic reagent. In addition to the above CE-LIF approaches, a fourth capillary electrophoretic tool is provided for the clinical chemist. Rapid analysis of biofluids is of significant importance in early disease diagnosis. As such, an extensive CE-based analysis of human seminal plasma is presented. Separation conditions, sample stability, and protein/non-protein zone identification issues are addressed. This study and the CE-LIF methodologies discussed above represent original approaches to nanoscale protein analysis.
Book Synopsis Protein Analysis by Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Franklin H. Welder
Download or read book Protein Analysis by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Franklin H. Welder and published by . This book was released on 2004 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Large Biomolecules by Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Pertti Juha Viskari
Download or read book Analysis of Large Biomolecules by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Pertti Juha Viskari and published by . This book was released on 2003 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Biomolecules Using Interfacial Capillary Electrophoresis with Laser Induced Fluorescence Detection by : Lorna Alice Hunter Pehl
Download or read book Analysis of Biomolecules Using Interfacial Capillary Electrophoresis with Laser Induced Fluorescence Detection written by Lorna Alice Hunter Pehl and published by . This book was released on 2000 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Nanoscale Biochemical Analysis Using Capillary Electrophoresis and Confocal Fluorescence Detection by : Anders Lundqvist
Download or read book Nanoscale Biochemical Analysis Using Capillary Electrophoresis and Confocal Fluorescence Detection written by Anders Lundqvist and published by . This book was released on 2002 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species by : Qifeng Xue
Download or read book Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species written by Qifeng Xue and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis with Post-column Laser-induced Fluorescence Detection of Proteins by :
Download or read book Capillary Electrophoresis with Post-column Laser-induced Fluorescence Detection of Proteins written by and published by . This book was released on 1996 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis with laser induced fluorescence detection (CE-LIF) is one of the alternative methods being developed for fast and sensitive assays of proteins and peptides. This report summarizes CE-LIF instrument development, efforts at labelling proteins, tests conducted to prove the applicability of CE-LIF to the analysis of samples, and results of a comparison of CE-LIF with some other techniques. Appendices include an instrument operating procedure and a paper that summarizes the experimental details of the protein labelling procedure and a CE-LIF technique for sub-nanomolar assay of proteins.
Book Synopsis Capillary Electrophoresis with Laser Induced Fluorescence as a Tool for Whole Cell and Marine Microbial Protein Analysis by : Kimberly Denise Chichester
Download or read book Capillary Electrophoresis with Laser Induced Fluorescence as a Tool for Whole Cell and Marine Microbial Protein Analysis written by Kimberly Denise Chichester and published by . This book was released on 2008 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection by : Sheri Joanne Lillard
Download or read book Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection written by Sheri Joanne Lillard and published by . This book was released on 1996 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Analysis of Limited Quantities of Amino Acids and Peptides Using Capillary Electrophoresis and Laser Induced Fluorescence Detection by : Beverly Nickerson
Download or read book The Analysis of Limited Quantities of Amino Acids and Peptides Using Capillary Electrophoresis and Laser Induced Fluorescence Detection written by Beverly Nickerson and published by . This book was released on 1991 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Two-wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides by : Guido Fridolin Verbeck
Download or read book Two-wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides written by Guido Fridolin Verbeck and published by . This book was released on 1999 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis High Sensitivity Protein Sequencing Using Fluorescein Isothiocyanate for Derivatization on a Miniaturized Sequencer Using Capillary Electrophoresis with Laser Induced Fluorescence Detection for Product Identification by :
Download or read book High Sensitivity Protein Sequencing Using Fluorescein Isothiocyanate for Derivatization on a Miniaturized Sequencer Using Capillary Electrophoresis with Laser Induced Fluorescence Detection for Product Identification written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis with Laser-induced Fluorescence Detection for the Determination of Amino Acid Neurotransmitters in Cerebrospinal Fluid by : Murali Seenappa
Download or read book Capillary Electrophoresis with Laser-induced Fluorescence Detection for the Determination of Amino Acid Neurotransmitters in Cerebrospinal Fluid written by Murali Seenappa and published by . This book was released on 2004 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Enzyme Assay by Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Yanni Zhang
Download or read book Enzyme Assay by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Yanni Zhang and published by . This book was released on 1996 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 994 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in Capillary Electrophoresis Analysis of Lipids, Proteins, and Peptides with Laser-induced Fluorescence by : Le Zhang
Download or read book Advances in Capillary Electrophoresis Analysis of Lipids, Proteins, and Peptides with Laser-induced Fluorescence written by Le Zhang and published by . This book was released on 2003 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Development of High Sensitivity Capillary Electrophoresis Fluorescence System for the Detection of Biological Molecules: by :
Download or read book Development of High Sensitivity Capillary Electrophoresis Fluorescence System for the Detection of Biological Molecules: written by and published by . This book was released on 2000 with total page 31 pages. Available in PDF, EPUB and Kindle. Book excerpt: One class of chemical & biological weapons consists of toxic proteins & peptides produced by biological organisms, or mid-spectrum agents. The research described in this report considered the analysis & detection of these mid-spectrum agents based on chemical methods, specifically via the use of capillary electrophoresis (CE) coupled with laser induced fluorescence (LIF). The report first presents a brief literature review and then highlights results of three research themes. Research papers detailing the experiments & results of these themes are appended. The first paper reports a method for assay of proteins at picomolar concentrations by CE, precolumn labelling, sub-micellar separation, and LIF detection. The second describes the use of sieving media for the separation of labeled proteins. The last paper addresses on-column labeling & analysis of proteins with a fluorogenic reagent. Overall results of the methods developed are compared to those achieved with ultraviolet absorbance detection.
