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Multiplexed Imaging
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Download or read book Multiplexed Imaging written by Eli Zamir and published by Humana. This book was released on 2021-08-31 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.
Download or read book Multiplexed Imaging written by Eli Zamir and published by Humana. This book was released on 2022-08-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.
Book Synopsis Massively Multiplexed Imaging Probes for Comprehensive Single-cell Analysis by : Sheldon J. J. Kwok
Download or read book Massively Multiplexed Imaging Probes for Comprehensive Single-cell Analysis written by Sheldon J. J. Kwok and published by . This book was released on 2019 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical microscopy techniques are widely used to study cellular physiology in their native tissue environments. In particular, the use of fluorescent probes to tag different cell populations, subcellular compartments, specific proteins and nucleotide sequences has enabled examination of cellular phenotypes with increasingly sophisticated detail. Recent efforts to combine physiological imaging and single-cell molecular analysis seek complete understanding of cellular identity and function within complex tissues. Specific cells of interest can be selected and isolated from tissues for downstream molecular analyses using techniques such as laser capture micro-dissection, or cell tagging with photo-conversion. However, high-throughput, unbiased molecular profiling of every cell imaged within a tissue remains an elusive challenge. A fundamental obstacle in previous approaches is spectral overlap due to the relatively broad emission of typical fluorescent probes, which limits their capabilities for multiplexed tagging. The first part of this thesis describes methods for studying cellular physiology in mice at single-cell resolution using two-photon fluorescence microscopy. The second part of this thesis describes the development of a novel class of imaging probes, called laser particles, which rely on narrowband laser emission for massively multiplexed cell tagging. This work establishes laser particles as promising tools for comprehensive single-cell analyses.
Book Synopsis Oncoimmunology by : Laurence Zitvogel
Download or read book Oncoimmunology written by Laurence Zitvogel and published by Springer. This book was released on 2017-12-13 with total page 700 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, leading experts in cancer immunotherapy join forces to provide a comprehensive guide that sets out the main principles of oncoimmunology and examines the latest advances and their implications for clinical practice, focusing in particular on drugs with FDA/EMA approvals and breakthrough status. The aim is to deliver a landmark educational tool that will serve as the definitive reference for MD and PhD students while also meeting the needs of established researchers and healthcare professionals. Immunotherapy-based approaches are now inducing long-lasting clinical responses across multiple histological types of neoplasia, in previously difficult-to-treat metastatic cancers. The future challenges for oncologists are to understand and exploit the cellular and molecular components of complex immune networks, to optimize combinatorial regimens, to avoid immune-related side effects, and to plan immunomonitoring studies for biomarker discovery. The editors hope that this book will guide future and established health professionals toward the effective application of cancer immunology and immunotherapy and contribute significantly to further progress in the field.
Book Synopsis Source Modulated Multiplexed Hyperspectral Imaging by : Lee Streeter
Download or read book Source Modulated Multiplexed Hyperspectral Imaging written by Lee Streeter and published by . This book was released on 2009 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Stimulated Raman Scattering Microscopy by : Ji-Xin Cheng
Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Book Synopsis A Demonstration of the Use of Walsh Functions for Multiplexed Imaging by : Robert Hugh McKenzie
Download or read book A Demonstration of the Use of Walsh Functions for Multiplexed Imaging written by Robert Hugh McKenzie and published by . This book was released on 1990 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Expansion Microscopy by : Fei Chen (Ph. D.)
Download or read book Expansion Microscopy written by Fei Chen (Ph. D.) and published by . This book was released on 2017 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microscopy has facilitated the discovery of many biological insights by optically magnifying small structures in cells and tissues. However, the resolution of optical microscopy is limited by the diffraction of light to ~200-300 nm, comparable or larger to the size of many subcellular structures. In this thesis, we describe a suite of tools based on a novel super-resolution microscopy approach called Expansion microscopy. Expansion microscopy (ExM) physically expands tissues so that the resolution of ordinary microscopes is increased -5 times by leveraging the swelling properties of polyelectrolyte hydrogels. Ordinary microscopes used with ExM are more accessible and faster than the specialized optical systems designed to image beyond the diffraction limit (e.g., STORM/PALM, STED, SIM), while yielding similar performance. Expanded tissues are also optically clear, allowing for unprecedented super-resolution imaging in thick tissues and facile reagent diffusion into the sample. We have since developed a variant of ExM, called protein retention ExM, in which proteins are directly anchored to the swellable gel using a commercially available cross-linking molecule. This strategy enables ExM of genetically encoded fluorescent proteins and commercial fluorescently labeled secondary antibodies. With these advancements, ExM can be carried out with purely commercial reagents and represents a simple extension of standard histological methods used to prepare samples for imaging. Furthermore, we have developed a variant of the ExM technology that enables RNA molecules to be directly linked to the ExM gel network via a small molecule linker and isotropic expansion. This technology, termed ExFISH, enables visualization of RNAs with nanoscale precision and single molecule resolution. We have demonstrated that the covalent anchoring of RNA also enables robust repeated washing and probe hybridization steps, opening the door to combinatorial multiplexing strategies. By leveraging these benefits, we have further developed in situ analysis tools which allow for highly multiplexed imaging of RNA identity and location with nanoscale precision in intact tissues. Taken together, these tools allow for spatially mapping molecular information onto cell types and tissue structures which could be invaluable for spatially complex biological processes such as brain function, cancer heterogeneity and organismal development.
Book Synopsis Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging by : Anna Siri Luthman
Download or read book Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging written by Anna Siri Luthman and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: And Rationale -- Spectral Imaging Systems and Sensor Characterisations -- Wide-field fHSI with a Linescan SRDA -- A Multispectral Endoscope Based on SRDAs -- Conclusions and Outlook.
Book Synopsis Multiplexed Hyperspectral Imaging and Spectrometry Using Spatial Light Modulators by : Richard A. DeVerse
Download or read book Multiplexed Hyperspectral Imaging and Spectrometry Using Spatial Light Modulators written by Richard A. DeVerse and published by . This book was released on 1999 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Secondary Ion Mass Spectrometry by : Robert G. Wilson
Download or read book Secondary Ion Mass Spectrometry written by Robert G. Wilson and published by Wiley-Interscience. This book was released on 1989-11-16 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Seco ndary Ion Mass Spectrometry Basic Concepts, Instrumental Aspects, Applications and Trends (Volume 86 in Chemical Analysis: A Series of Monographs on Analytical Chemistry and Its Applications) A. Benninghoven, F. G. Rüdenauer, and H. W. Werner "[This book] is (and probably will be for a long time ahead) the standard book on secondary ion mass spectrometry." —Trends in Analytical Chemistry "This is a monumental work, and contains nearly 600 illustrations and over 2,000 references covering nearly all the essential published information up to 1985. The book will certainly find its place as a reference work in most laboratories using this methodology" —Analytica Chimica Acta 1987 (0 471-01056-1) 1,227 pp. Secondary Ion Mass Spectrometry Proceedings of the Sixth International Conference on Secondary Ion Mass Spectrometry (SIMS VI) Edited by A. Benninghoven, A.M. Huber, and H. W. Werner "The international SIMS conferences have been held every two years since 1977. They are recognized as one of the major forums for scientists, instrument manufacturers, and other researchers actively engaged in this rapidly expanding field…this volume is a valuable account of the latest advances in the field of SIMS, and of the research trends of some of the most respected experts in the field.…it is recommended for the libraries of all academic and industrial institutions where SIMS research is ongoing.…it should prove a valuable reference source for years to come." —Applied Spectroscopy 1988 (0 471-91832-6) 1,078 pp.
Book Synopsis Deciphering and Modelling the Action of Immune Cells Using Highly Multiplexed Imaging and Deep Learning Techniques by : Clinton Reid
Download or read book Deciphering and Modelling the Action of Immune Cells Using Highly Multiplexed Imaging and Deep Learning Techniques written by Clinton Reid and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells of the immune system are capable of responding to foreign antigen, promoting host defense while limiting damage to host tissues, through an act known as self-tolerance. T cells, their activation and their effector roles are of particular interest due to their prominent roles in antigen discrimination and subsequent cell-mediated immunity. However, there are diverse effector T cell types interacting to regulate the immune response. Understanding the mechanisms by which intercellular interactions exert precise control over the immune system is a crucial step in elucidating the manner in which the immune system behaves during infection, health, or chronic disease. Multiplexed imaging is a beneficial tool that is used to visualize distinct cell types and functional states directly in tissues. This technology is particularly important for understanding how cells organize spatially to enforce this boundary between host-protective responses and autoimmunity. Therefore, it is valuable to image interacting cells in highly-multiplexed images. In order to do this, it has become increasingly important to increase the number of biomarkers that one can record in a single tissue section at a time. Here, I summarize our efforts to employ imaging and deep learning tools to analyze the structure of the immune system, ending with a critical insight regarding our cell segmentation models alongside an experimental workflow and pipeline that will allow even more to be revealed about the mechanisms of control that exist within the immune system. Current methods for acquiring highly multiplexed images are somewhat time-consuming and labour-intensive while computational methods for analyzing these images and identifying relevant spatial patterns are lacking. We seek to improve and simplify our current multiplexing capabilities by eventually coupling fluorescence lifetime with fluorescence intensity measurements-two distinct imaging modalities. Moreover, we aim to develop new computational pipelines to aid in down-stream image analysis and identify new spatial motifs that control immune response in tissues.
Book Synopsis Revealing Uncharted Biology with Single Cell Multiplex Proteomic Technologies by : Wendy Fantl
Download or read book Revealing Uncharted Biology with Single Cell Multiplex Proteomic Technologies written by Wendy Fantl and published by Academic Press. This book was released on 2024-06-28 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revealing Unchartered Biology with Single Intact Cells: Case Studies explores the path to research success, key projects, the role of techniques, the selection process, other alternatives considered, what other paths have led to dead ends, detailed protocols followed, and how the analysis of generated data allowed researchers to visualize unchartered biology. Focusing on the research journey that led to the publication of each article, the book's editors interviewed the researchers on the use of the Multiplex Single Cell technique and how it helped hone in on the biological quest. These methods can be expanded to a wide variety of research objectives. In conclusion to each chapter, the authors critically review their process and provide suggestions of improvement or alternate techniques that could be employed. This book is the ideal reference for researchers new to the world of single-cell multiplex techniques. The discussion on failures encountered along the research path provides insights on how to avoid repeating the same errors. Provides insights into the path to success of key research articles based on Multiplex Single-Cell analysis techniques results Contains detailed method information Discusses strengths and limitations of techniques applied to each research domain covered Includes discussions on the failures encountered along the research path and how to avoid them
Book Synopsis Highly-multiplexed Imaging Analysis of Intra-tumour Heterogeneity and Response to Immune Checkpoint Inhibitors in Colorectal Cancer by : Michele Bortolomeazzi
Download or read book Highly-multiplexed Imaging Analysis of Intra-tumour Heterogeneity and Response to Immune Checkpoint Inhibitors in Colorectal Cancer written by Michele Bortolomeazzi and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging by : Anna Siri Luthman
Download or read book Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging written by Anna Siri Luthman and published by Springer. This book was released on 2018-10-09 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the design, development, characterisation and application of two novel fluorescence imaging instruments based on spectrally resolved detector arrays (SRDAs). The simplest SRDA is the standard colour camera, which integrates a Bayer filter array of red, green and blue colour filters to replicate the colour sensing capability of the human eye. The SRDAs used in this book contain many more colours, ranging from 16 to over 100 colour channels. Using these compact, robust and low-cost detectors for biomedical applications opens new avenues of exploration that were not possible before, in particular, the use of spectral imaging in endoscopy. The work presented shows for the first time that not only can this new type of camera be used for fluorescence imaging, but also that it is able to resolve signals from up to 7 different dyes – a level of multiplexing not previously achieved in tissue with such compact and robust equipment. Furthermore, it reports the application of a bimodal endoscope performing both reflectance and fluorescence imaging using these cameras in an ex vivo pig oesophagus model.
Book Synopsis Advanced Multiplexing Techniques in Single Molecule Localisation Microscopy by : Nazar Oleksiievets
Download or read book Advanced Multiplexing Techniques in Single Molecule Localisation Microscopy written by Nazar Oleksiievets and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging applications. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. This thesis presents several novel techniques for multiplexed imaging, with the main focus on the DNA-PAINT super-resolution technique. At first, I demonstrate multiplexed imaging by using three different single-domain antibodies (nanobodies). They have a three times smaller size than conventiona...
Book Synopsis Multiplex Imaging and Random Arrays by : Christopher M. Brown
Download or read book Multiplex Imaging and Random Arrays written by Christopher M. Brown and published by . This book was released on 1972 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
