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Author : Ailsa M. Campbell
Publisher :
ISBN 13 :
Total Pages : 265 pages
Book Rating : 4.:/5 (78 download)
Download or read book Monoclonal Antibody Technology written by Ailsa M. Campbell and published by . This book was released on 1987 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ailsa M. Campbell
Publisher :
ISBN 13 : 9780720442007
Total Pages : 265 pages
Book Rating : 4.4/5 (42 download)
Download or read book Monoclonal Antibody Technology written by Ailsa M. Campbell and published by . This book was released on 1987 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ailsa M. Campbell
Publisher :
ISBN 13 : 9780444805928
Total Pages : 265 pages
Book Rating : 4.8/5 (59 download)
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by Ailsa M. Campbell and published by . This book was released on 1969 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.
Author :
Publisher :
ISBN 13 : 9780444805751
Total Pages : 265 pages
Book Rating : 4.8/5 (57 download)
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by and published by . This book was released on 1984 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : A.M. Campbell
Publisher : Elsevier
ISBN 13 : 9780080858821
Total Pages : 264 pages
Book Rating : 4.8/5 (588 download)
Download or read book Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas written by A.M. Campbell and published by Elsevier. This book was released on 2000-04-01 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.
Author : A.M. Campbell
Publisher : Elsevier
ISBN 13 : 9780080887357
Total Pages : 426 pages
Book Rating : 4.8/5 (873 download)
Download or read book Monoclonal Antibody and Immunosensor Technology written by A.M. Campbell and published by Elsevier. This book was released on 1991-12-12 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene transformation and an interesting section on Mab patents.
Author : Ailsa M. Campbell
Publisher :
ISBN 13 : 9780720442007
Total Pages : 427 pages
Book Rating : 4.4/5 (42 download)
Download or read book Monoclonal Antibody and Immunosensor Technology written by Ailsa M. Campbell and published by . This book was released on 1991 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Edgar Engleman
Publisher : Springer Science & Business Media
ISBN 13 : 1468449494
Total Pages : 528 pages
Book Rating : 4.4/5 (684 download)
Download or read book Human Hybridomas and Monoclonal Antibodies written by Edgar Engleman and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech nical problems, and the first reports of hybrids secreting human immu noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.
Author : John G.R. Hurrell
Publisher : CRC Press
ISBN 13 : 1351083279
Total Pages : 239 pages
Book Rating : 4.3/5 (51 download)
Download or read book Monoclonal Hybridoma Antibodies written by John G.R. Hurrell and published by CRC Press. This book was released on 2018-05-04 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume.
Author :
Publisher :
ISBN 13 : 9780720442007
Total Pages : 427 pages
Book Rating : 4.4/5 (42 download)
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by and published by . This book was released on 1991 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309173051
Total Pages : 74 pages
Book Rating : 4.3/5 (91 download)
Download or read book Monoclonal Antibody Production written by National Research Council and published by National Academies Press. This book was released on 1999-05-06 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.
Author : Roger H. Kennett
Publisher : Springer Science & Business Media
ISBN 13 : 1461575052
Total Pages : 423 pages
Book Rating : 4.4/5 (615 download)
Download or read book Monoclonal Antibodies written by Roger H. Kennett and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: On August 7, 1975, Kohler and Milstein published in Nature (256:495) a report describing "Continuous cultures of fused cells secreting antibody of predefined specificity. " Their report has become a classic and has already had a profound effect on basic and applied research in biology and medicine. By the time the first Workshop on Lymphocyte Hybridomas (Current Topics in Microbiology and Im munology 81, 1978) was held on April 3-5, 1978, in Bethesda, Maryland, investi gators from many laboratories had made hybrids between plasmacytomas and spleen cells from immunized animals and had obtained monoclonal antibodies reacting with a broad variety of antigenic determinants. At the time Kohler and Milstein introduced this new technology, the editors of this volume were involved in the production of antisera against differentiation antigens (K. B. B. ), histocompatibility antigens (T. ]. McK. ), and human tumor associated antigens (R. H. K. ). Because of the potential usefulness of monoclonal antibodies in these areas, we each began production of hybridomas and analysis of the resulting monoclonal reagents. One of the most interesting aspects of participation in the early stages of the development and application of hybrid oma technology has been observing how the implications of the initial observa tions gradually spread first among the practitioners of immunology and immu nogenetics, and then to other areas of the biological sciences, such as developmental biology, biochemistry, human genetics, and cell and tumor biology.
Author : Glen Christopher MacDonald
Publisher :
ISBN 13 :
Total Pages : 338 pages
Book Rating : 4.:/5 (184 download)
Download or read book The Production and Characterization of Human-human and Human-mouse Rh(D) Specific Monoclonal Antibodies written by Glen Christopher MacDonald and published by . This book was released on 1987 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Timothy Springer
Publisher : Springer Science & Business Media
ISBN 13 : 1468449648
Total Pages : 603 pages
Book Rating : 4.4/5 (684 download)
Download or read book Hybridoma Technology in the Biosciences and Medicine written by Timothy Springer and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : U. S. Singh
Publisher : CRC Press
ISBN 13 : 1351430211
Total Pages : 836 pages
Book Rating : 4.3/5 (514 download)
Download or read book Molecular Methods in Plant Pathology written by U. S. Singh and published by CRC Press. This book was released on 2017-12-14 with total page 836 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.
Author : Vincent Ossipow
Publisher : Humana
ISBN 13 : 9781493963225
Total Pages : 0 pages
Book Rating : 4.9/5 (632 download)
Download or read book Monoclonal Antibodies written by Vincent Ossipow and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.
Author : Food and Agriculture Organization of the United Nations. Animal Production and Health Division
Publisher : Springer Science & Business Media
ISBN 13 : 9780306432064
Total Pages : 478 pages
Book Rating : 4.4/5 (32 download)
Download or read book Biotechnology for Livestock Production written by Food and Agriculture Organization of the United Nations. Animal Production and Health Division and published by Springer Science & Business Media. This book was released on 1989-05-31 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the expert consultation prepared by the Animal Production and Health Division, FHO. Topics covered by the contributors include: biotechnology the frontiers of knowledge and methodologies, animal reproduction, animal genetics, animal growth, lactation, and fiber production, animal nutr
