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Author : Deirdre O'Meara
Publisher :
ISBN 13 : 9789172831612
Total Pages : 71 pages
Book Rating : 4.8/5 (316 download)
Download or read book Molecular Tools for Nucleic Acid Analysis written by Deirdre O'Meara and published by . This book was released on 2001 with total page 71 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jacquie T. Keer
Publisher : Royal Society of Chemistry
ISBN 13 : 0854043675
Total Pages : 274 pages
Book Rating : 4.8/5 (54 download)
Download or read book Essentials of Nucleic Acid Analysis written by Jacquie T. Keer and published by Royal Society of Chemistry. This book was released on 2008 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: An indispensable handbook of the highest standard for those working in the fields of food analysis and forensic applications.
Author : Oana-Maria Boldura
Publisher : BoD – Books on Demand
ISBN 13 : 1789848563
Total Pages : 207 pages
Book Rating : 4.7/5 (898 download)
Download or read book Biochemical Analysis Tools written by Oana-Maria Boldura and published by BoD – Books on Demand. This book was released on 2020-06-24 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores the role of nucleic acid analysis and the advances it has led to in the field of life sciences. The first section is a collection of chapters covering experimental methods used in molecular biology, the techniques adjacent to these methods, and the steps of analysis before and after obtaining raw DNA data. The second section deals with the principles of chromatography, method development, sample preparation, and industrial applications.
Author : Elena Hilario
Publisher : Springer Science & Business Media
ISBN 13 : 1597452297
Total Pages : 318 pages
Book Rating : 4.5/5 (974 download)
Download or read book Protocols for Nucleic Acid Analysis by Nonradioactive Probes written by Elena Hilario and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author : David Malcolm James Lilley
Publisher : Birkhauser
ISBN 13 :
Total Pages : 488 pages
Book Rating : 4.3/5 (91 download)
Download or read book Structural Tools for the Analysis of Protein-nucleic Acid Complexes written by David Malcolm James Lilley and published by Birkhauser. This book was released on 1992 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Marcelo Larramendy
Publisher : BoD – Books on Demand
ISBN 13 : 9535122649
Total Pages : 222 pages
Book Rating : 4.5/5 (351 download)
Download or read book Nucleic Acids written by Marcelo Larramendy and published by BoD – Books on Demand. This book was released on 2016-03-16 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book, "Nucleic Acids - From Basic Aspects to Laboratory Tools", contains a series of chapters that highlight the development and status of the various aspects of the nucleic acids related to DNA chemistry and biology and the molecular application of these small DNA molecules and related synthetic analogues within biological systems. Furthermore, it is hoped that the information in the present book will be of value to those directly engaged in the handling and use of nucleic acids, and that this book will continue to meet the expectations and needs of all who are interested in the different fascinating aspects of molecular biology.
Author : David Edwards
Publisher : Springer Science & Business Media
ISBN 13 : 0387927387
Total Pages : 450 pages
Book Rating : 4.3/5 (879 download)
Download or read book Bioinformatics written by David Edwards and published by Springer Science & Business Media. This book was released on 2009-09-03 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioinformatics is a relatively new field of research. It evolved from the requirement to process, characterize, and apply the information being produced by DNA sequencing technology. The production of DNA sequence data continues to grow exponentially. At the same time, improved bioinformatics such as faster DNA sequence search methods have been combined with increasingly powerful computer systems to process this information. Methods are being developed for the ever more detailed quantification of gene expression, providing an insight into the function of the newly discovered genes, while molecular genetic tools provide a link between these genes and heritable traits. Genetic tests are now available to determine the likelihood of suffering specific ailments and can predict how plant cultivars may respond to the environment. The steps in the translation of the genetic blueprint to the observed phenotype is being increasingly understood through proteome, metabolome and phenome analysis, all underpinned by advances in bioinformatics. Bioinformatics is becoming increasingly central to the study of biology, and a day at a computer can often save a year or more in the laboratory. The volume is intended for graduate-level biology students as well as researchers who wish to gain a better understanding of applied bioinformatics and who wish to use bioinformatics technologies to assist in their research. The volume would also be of value to bioinformatics developers, particularly those from a computing background, who would like to understand the application of computational tools for biological research. Each chapter would include a comprehensive introduction giving an overview of the fundamentals, aimed at introducing graduate students and researchers from diverse backgrounds to the field and bring them up-to-date on the current state of knowledge. To accommodate the broad range of topics in applied bioinformatics, chapters have been grouped into themes: gene and genome analysis, molecular genetic analysis, gene expression analysis, protein and proteome analysis, metabolome analysis, phenome data analysis, literature mining and bioinformatics tool development. Each chapter and theme provides an introduction to the biology behind the data describes the requirements for data processing and details some of the methods applied to the data to enhance biological understanding.
Author : E. van Pelt-Verkuil
Publisher : Springer
ISBN 13 : 981131604X
Total Pages : 457 pages
Book Rating : 4.8/5 (113 download)
Download or read book Molecular Diagnostics written by E. van Pelt-Verkuil and published by Springer. This book was released on 2019-06-03 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: The books Molecular Diagnostics Part 1 and 2 provide a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are being used in a wide variety of disciplines. The editors and experts in their respective fields have combined their knowledge to write these two books. Many years of experience in the development, application and quality control of molecular diagnostic methods is reflected herewith. Molecular Diagnostics Part 1 is dedicated to the theoretical backgrounds of the technologies often applied in molecular diagnostics, in which nucleic acid amplification methods (such as real-time PCR), sequencing and bioinformatics are the basic tools. The assay design and -development, combined with items of trouble-shooting are described in detail. As a foundation of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is thoroughly discussed. This book also provides extensive information for those working with molecular techniques in a wide variety of research applications using conventional and real-time PCR technology, Sanger and high throughput sequencing techniques, and bioinformatics. Molecular Diagnostics Part 2 highlights the applications of the molecular diagnostic methods in the various diagnostic laboratories, comprising: - Clinical microbiology - Clinical chemistry - Clinical genetics - Clinical pathology - Molecular hematopathology - Veterinary health - Plant health - Food safety Both full-colour and well-illustrated books are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic methods and for those who wish to broaden their knowledge on the current molecular biological revolution. The information in the books highlights the trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.
Author : Iddo Heller
Publisher : Springer Nature
ISBN 13 : 1071633775
Total Pages : 511 pages
Book Rating : 4.0/5 (716 download)
Download or read book Single Molecule Analysis written by Iddo Heller and published by Springer Nature. This book was released on 2023-11-13 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author : G. Subramanian
Publisher :
ISBN 13 : 9781578080359
Total Pages : 0 pages
Book Rating : 4.0/5 (83 download)
Download or read book Cyanobacterial Biotechnology written by G. Subramanian and published by . This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume comprises select papers presented at a symposium held in September, 1996 in India. The contributors used the forum to disseminate their research findings.
Author : A. R. Hoelzel
Publisher : Oxford University Press
ISBN 13 : 0191565601
Total Pages : 468 pages
Book Rating : 4.1/5 (915 download)
Download or read book Molecular Genetic Analysis of Populations written by A. R. Hoelzel and published by Oxford University Press. This book was released on 1998-03-19 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods enabling the direct study of genetic variation in natural populations have improved considerably. The new edition explores these updated techniques in DNA analysis and provides a revised and refined laboratory guide to investigating variation in DNA molecules.
Author : Rainer Kurmayer
Publisher : John Wiley & Sons
ISBN 13 : 1119332109
Total Pages : 446 pages
Book Rating : 4.1/5 (193 download)
Download or read book Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria written by Rainer Kurmayer and published by John Wiley & Sons. This book was released on 2017-09-05 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.
Author : Oana-Maria Boldura
Publisher :
ISBN 13 : 9781838809034
Total Pages : pages
Book Rating : 4.8/5 (9 download)
Download or read book Biochemical Analysis Tools written by Oana-Maria Boldura and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Nader Rifai
Publisher : Elsevier
ISBN 13 : 0128160624
Total Pages : 422 pages
Book Rating : 4.1/5 (281 download)
Download or read book Principles and Applications of Molecular Diagnostics written by Nader Rifai and published by Elsevier. This book was released on 2018-06-13 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. Explains the principles and tools of molecular biology Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results Provides a detailed description of current molecular applications used to solve diagnostics tasks
Author : Charles A. Dangler
Publisher : Wiley-Liss
ISBN 13 :
Total Pages : 304 pages
Book Rating : 4.3/5 (91 download)
Download or read book Nucleic Acid Analysis written by Charles A. Dangler and published by Wiley-Liss. This book was released on 1996-06-14 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic acid hybridizations: principles and strategies; Generation of nucleic acid probe molecules; Nonisotopic DNA labeling strategies; Approaches to genetic analysis: genetic disease and screening; Approaches to genetic analysis: forensics; Approaches to infectious disease diagnosis; Diagnostic nucleic acid probe technology in veterinary medicine; Diagnostic nucleic acid probes in human medicine: applications, present and future; Developments in food technology: applications and regulatory and economic considerations; Current development and applications of nucleic acid technology in the environmental sciences.
Author : Gustavo Caetano-Anollés
Publisher : Wiley-Liss
ISBN 13 :
Total Pages : 392 pages
Book Rating : 4.3/5 (91 download)
Download or read book DNA Markers written by Gustavo Caetano-Anollés and published by Wiley-Liss. This book was released on 1997-09-16 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the four decades since the discovery of DNA, molecular genetics and biotechnology have undergone a revolution in the research and application of genetic testing techniques. As a result, these fields have witnessed an explosion of both information and controversy, and the focus of public awareness of the significance of molecular biotechnology in everyday life has leaped from journal abstracts to front-page news. Molecular biotechnology provides important tools for the identification of specific nucleic acid sequences, some of them linked to important traits such as those conferring resistance to disease. Such DNA markers are used commonly in genetic research and in other applications, such as DNA fingerprinting. Researchers can use DNA markers to follow individual traits in different environments and hosts, increasing our understanding of the constitution, diversity, and evolution of genetic material. DNA Markers: Protocols, Applications, and Overviews offers valuable new information on this rapidly growing field. This compendium of research highlights the most recently discovered DNA markers and their application to the analyses of genomes of low and high complexity, with a focus on recently developed DNA marker systems as tools for DNA fingerprinting, genome analysis, molecular systematics, taxonomy, and phylogenetic analysis. The Protocols section of the book contains detailed, step-by-step instructions on how to perform selected DNA marker experiments, providing a useful laboratory tool. Subjects covered by top specialists include: Designed as a bench-top reference for the laboratory as well as a complete guide to the latest research, DNA Markers: Protocols, Applications, and Overviews is a vital resource for both novices and experienced practitioners involved in research in molecular genetics or biotechnology. A global overview of DNA markers * Molecular hybridization * RAPD, AP-PCR, DAF and AFLP analysis * Microbial Genomic fingerprinting * Simple sequence repeat DNA markers * Genetic BIT analysis * Differential display of RNA * The status of DNA fingerprinting: population databases * Arbitrarily amplified DNA in ecology and evolution
Author : Dirk Lassner
Publisher : Springer
ISBN 13 : 1461553792
Total Pages : 143 pages
Book Rating : 4.4/5 (615 download)
Download or read book Modern Applications of DNA Amplification Techniques written by Dirk Lassner and published by Springer. This book was released on 2013-11-11 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been on human microbiology and oncological problems. The exquisite sensitivity of PCR gives this method the ability to detect extremely rare DNAs, mRNAs, mRNAs in small numbers of cells or in small amounts of tissue, and mRNAs expressed in mixed-cell populations. However, the exact and accurate quantification of specific nucleic acids in biological samples is in spite of numerous publications in that field still a general problem: during the peR process, an unknown initial number of target sequences are used as a template from which a large quantity of specific product can be obtained. Although the amount of product formed is easy to determine, it is difficult to deduce the initial copy number of the target molecule because the efficiency of the peR is largely unknown.
