Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

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Publisher : Humana Press
ISBN 13 : 9781588297006
Total Pages : 266 pages
Book Rating : 4.2/5 (97 download)

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Book Synopsis Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols by : Andreas Marx

Download or read book Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols written by Andreas Marx and published by Humana Press. This book was released on 2008-04-03 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.

Hedgehog Signaling Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 158829692X
Total Pages : 267 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Hedgehog Signaling Protocols by : Jamila I. Horabin

Download or read book Hedgehog Signaling Protocols written by Jamila I. Horabin and published by Springer Science & Business Media. This book was released on 2007-09-13 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.

Neuroinformatics

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Publisher : Springer Science & Business Media
ISBN 13 : 1588297209
Total Pages : 419 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Neuroinformatics by : Chiquito J. Crasto

Download or read book Neuroinformatics written by Chiquito J. Crasto and published by Springer Science & Business Media. This book was released on 2007-11-29 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neuroinformatics presents cutting-edge techniques for the synergistic study of neuroinformatics. The book facilitates the efforts of discovering neuroscience through the sharing of data and the use of computational models. It demonstrates the use of neuroinformatic components as a mechanism for understanding complex disorders. It contains detailed explanations, advantages, and disadvantages of traditional and non-invasive imaging methods.

Hematopoietic Stem Cell Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 158829868X
Total Pages : 306 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Hematopoietic Stem Cell Protocols by : Kevin D. Bunting

Download or read book Hematopoietic Stem Cell Protocols written by Kevin D. Bunting and published by Springer Science & Business Media. This book was released on 2008-01-31 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: This revised edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. The book provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations, and offers a variety of transplantation approaches to measure HSC function in vivo. This is a much needed technical resource in the critically important field of stem cell investigation.

RNA Chaperones

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Publisher :
ISBN 13 : 9781071602317
Total Pages : 314 pages
Book Rating : 4.6/5 (23 download)

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Book Synopsis RNA Chaperones by : Tilman Heise

Download or read book RNA Chaperones written by Tilman Heise and published by . This book was released on 2020 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve into the complex interactions between RNA chaperones and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Chaperones: Methods and Protocols serves as an ideal guide for scientists and students interested in RNA biology and RNA chaperones. Chapter 3 is available Open Access under a CC-BY 4.0 license via link.springer.com.

Nucleic Acid Detection

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Publisher : Humana Press
ISBN 13 : 9781627035347
Total Pages : 0 pages
Book Rating : 4.0/5 (353 download)

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Book Synopsis Nucleic Acid Detection by : Dmitry M. Kolpashchikov

Download or read book Nucleic Acid Detection written by Dmitry M. Kolpashchikov and published by Humana Press. This book was released on 2013-09-13 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

Telomerase Inhibition

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Publisher : Springer Science & Business Media
ISBN 13 : 1588296830
Total Pages : 197 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Telomerase Inhibition by : Lucy Andrews

Download or read book Telomerase Inhibition written by Lucy Andrews and published by Springer Science & Business Media. This book was released on 2007-11-29 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition. The techniques described provide the researcher with a diverse and comprehensive set of tools for the study of telomerase inhibition. The volume is aimed at biochemists, molecular biologists, cancer researchers, and geneticists.

Clinical Proteomics

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Publisher : Springer Science & Business Media
ISBN 13 : 158829837X
Total Pages : 401 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Clinical Proteomics by : Antonia Vlahou

Download or read book Clinical Proteomics written by Antonia Vlahou and published by Springer Science & Business Media. This book was released on 2008-01-18 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.

Organelle Proteomics

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Publisher : Springer Science & Business Media
ISBN 13 : 1588297799
Total Pages : 423 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Organelle Proteomics by : Delphine Pflieger

Download or read book Organelle Proteomics written by Delphine Pflieger and published by Springer Science & Business Media. This book was released on 2008-02-19 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to examine organelle proteomics in depth. It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis. Detailed protocols are provided and a section is devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle.

Gene Function Analysis

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Publisher : Springer Science & Business Media
ISBN 13 : 1588297349
Total Pages : 703 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Gene Function Analysis by : Michael F. Ochs

Download or read book Gene Function Analysis written by Michael F. Ochs and published by Springer Science & Business Media. This book was released on 2007-08-23 with total page 703 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the advent of high-throughput technologies following completion of the human genome project and similar projects, the number of genes of interest has expanded and the traditional methods for gene function analysis cannot achieve the throughput necessary for large-scale exploration. This book brings together a number of recently developed techniques for looking at gene function, including computational, biochemical and biological methods and protocols.

Alcohol

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Publisher : Springer Science & Business Media
ISBN 13 : 1588299066
Total Pages : 418 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Alcohol by : Laura E. Nagy

Download or read book Alcohol written by Laura E. Nagy and published by Springer Science & Business Media. This book was released on 2008-03-07 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the pleiotropic effects of ethanol in animal and cell culture models through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.

The Handbook of Plant Mutation Screening

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Publisher : John Wiley & Sons
ISBN 13 : 9783527629404
Total Pages : 460 pages
Book Rating : 4.6/5 (294 download)

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Book Synopsis The Handbook of Plant Mutation Screening by : Khalid Meksem

Download or read book The Handbook of Plant Mutation Screening written by Khalid Meksem and published by John Wiley & Sons. This book was released on 2009-12-21 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Induced mutagenesis is a common and promising method for screening for new crops with improved properties. This title introduces the different methods and then focuses on the screening, detection and analysis of the novel mutations. Written by a global team of authors the book is an indispensable tool for all scientists working on crop breeding in industry and academia.

Affinity Chromatography

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Publisher : Springer Science & Business Media
ISBN 13 : 1588296598
Total Pages : 341 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Affinity Chromatography by : Michael Zachariou

Download or read book Affinity Chromatography written by Michael Zachariou and published by Springer Science & Business Media. This book was released on 2008 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers and development scientists whilst expanding on some more traditional affinity chromatography approaches that have become of greater interest to separation scientists. Chapters in this cutting-edge text expand on affinity chromatography techniques that currently enjoy frequent citation in the literature from those purifying biomolecules. Other chapters include protocols describing the use of a variety of fusion tags as well as how to cleave them, so as to allow the scientists to study the native phenotype of the protein. Renowned researchers also include protocols detailing diverse applications of affinity chromatography such as its use in catalytic reactions, DNA purification, whole cell separations and for the isolation of phosphorylated proteins. Affinity Chromatography: Methods and Protocols, Second Edition, is an essential reference for those interested in separation sciences, particularly in the pharmaceutical and biological research sectors, that have an interest in isolating macromolecules rapidly, quantitatively, and with high purity.

Bacterial Pathogenesis

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Publisher : Springer Science & Business Media
ISBN 13 : 1588297403
Total Pages : 314 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Bacterial Pathogenesis by : Frank R. DeLeo

Download or read book Bacterial Pathogenesis written by Frank R. DeLeo and published by Springer Science & Business Media. This book was released on 2008-01-31 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial infections affect world health today as a leading cause of morbidity and mortality. This book presents in-depth methods and state-of-the-art protocols for investigating specific mechanisms of pathogenesis for a wide range of bacteria. Written by experts in the field, this invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections.

Exocytosis and Endocytosis

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Publisher : Springer Science & Business Media
ISBN 13 : 1588298655
Total Pages : 407 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Exocytosis and Endocytosis by : Andrei I. Ivanov

Download or read book Exocytosis and Endocytosis written by Andrei I. Ivanov and published by Springer Science & Business Media. This book was released on 2008 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, skilled experts provide the most up-to-date, step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. The book is insightful to both newcomers and seasoned professionals. It offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.

Plant Embryogenesis

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Publisher : Springer Science & Business Media
ISBN 13 : 1588299317
Total Pages : 184 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Plant Embryogenesis by : María F. Suárez

Download or read book Plant Embryogenesis written by María F. Suárez and published by Springer Science & Business Media. This book was released on 2008 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Step-by-step reproducible laboratory procedures written by the top experts in the field offering their time-tested hints, tricks, and tips. Presents detailed outlines of embryonic systems models along with cutting-edge cellular, genetic, and molecular mechanism. Provides the most comprehensive collection of protocols on plan embryo development.

Reporter Genes

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Publisher : Springer Science & Business Media
ISBN 13 : 158829739X
Total Pages : 165 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Reporter Genes by : Donald S. Anson

Download or read book Reporter Genes written by Donald S. Anson and published by Springer Science & Business Media. This book was released on 2007-09-13 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.