Molecular Assemblies Observed by Atomic Force Microscopy

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ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (184 download)

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Book Synopsis Molecular Assemblies Observed by Atomic Force Microscopy by : David Alejandro Cisneros Armas

Download or read book Molecular Assemblies Observed by Atomic Force Microscopy written by David Alejandro Cisneros Armas and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Assemblies Observed by Atomic Force Microscopy

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (652 download)

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Book Synopsis Molecular Assemblies Observed by Atomic Force Microscopy by :

Download or read book Molecular Assemblies Observed by Atomic Force Microscopy written by and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: We use time-lapse AFM to visualize collagen fibrils self-assembly. A solution of acid-solubilized collagen was injected into the AFM fluid cell and fibril formation was observed in vitro. Single fibrils continuously grew and fused with each other until the supporting surface was completely covered by a nanoscopically well-defined collagen matrix. Laterally, the fibrils grew in steps of ~4 nm suggesting a two-step mechanism. In a first step, collagen molecules associated together. In the second step, these molecules rearranged into a structure called a microfibril. High-resolution AFM topographs revealed substructural details of the D-band architecture. These substructures correlated well with those revealed from positively stained collagen fibers imaged by transmission electron microscopy. Secondly, a covalent assembly approach to prepare membrane protein for AFM imaging that avoids crystallization was proposed. High-resolution AFM topographs can reveal structural details of single membrane proteins but, as a prerequisite, the proteins must be adsorbed to atomically flat mica and densely packed in a membrane to restrict their lateral mobility. Atomically flat gold, engineered proteins, and chemically modified lipids were combined to rapidly assemble immobile and fully oriented samples. The resulting AFM topographs of single membrane proteins were used to create averaged structures with a resolution approaching that of 2D crystals. Finally, the contribution of specific amino acid residues to the stability of membrane proteins was studied. Two structurally similar proteins sharing only 30% sequence identity were compared. Single-molecule atomic force microscopy and spectroscopy was used to detect molecular interactions stabilizing halorhodopsin (HR) and bacteriorhodopsin (BR). Their unfolding pathways and polypeptide regions that established stable segments were compared. Both proteins unfolded exactly via the same intermediates. This 3 Molecular Assemblies observed b.

Local Mechanical and Electrical Measurements on Molecular Assemblies Using Atomic Force Microscopy

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ISBN 13 :
Total Pages : 116 pages
Book Rating : 4.:/5 (856 download)

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Book Synopsis Local Mechanical and Electrical Measurements on Molecular Assemblies Using Atomic Force Microscopy by : Carsten Hentschel

Download or read book Local Mechanical and Electrical Measurements on Molecular Assemblies Using Atomic Force Microscopy written by Carsten Hentschel and published by . This book was released on 2012 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Assemblies of Membrane Remodelling Studied by Atomic Force Microscopy

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (89 download)

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Book Synopsis Molecular Assemblies of Membrane Remodelling Studied by Atomic Force Microscopy by : Ian William Fyfe

Download or read book Molecular Assemblies of Membrane Remodelling Studied by Atomic Force Microscopy written by Ian William Fyfe and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Manipulation with Atomic Force Microscopy

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Publisher : CRC Press
ISBN 13 : 1439809674
Total Pages : 285 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Molecular Manipulation with Atomic Force Microscopy by : Anne-Sophie Duwez

Download or read book Molecular Manipulation with Atomic Force Microscopy written by Anne-Sophie Duwez and published by CRC Press. This book was released on 2011-12-07 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the invention of scanning probe techniques in the early 1980s, scientists can now play with single atoms, single molecules, and even single bonds. Force, dynamics, and function can now be probed at the single-molecule level. Molecular Manipulation with Atomic Force Microscopy (AFM) presents a series of topics that discuss concepts and methodol

Noncontact Atomic Force Microscopy

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Publisher : Springer
ISBN 13 : 3319155881
Total Pages : 539 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Noncontact Atomic Force Microscopy by : Seizo Morita

Download or read book Noncontact Atomic Force Microscopy written by Seizo Morita and published by Springer. This book was released on 2015-05-18 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest developments in noncontact atomic force microscopy. It deals with the following outstanding functions and applications that have been obtained with atomic resolution after the publication of volume 2: (1) Pauli repulsive force imaging of molecular structure, (2) Applications of force spectroscopy and force mapping with atomic resolution, (3) Applications of tuning forks, (4) Applications of atomic/molecular manipulation, (5) Applications of magnetic exchange force microscopy, (6) Applications of atomic and molecular imaging in liquids, (7) Applications of combined AFM/STM with atomic resolution, and (8) New technologies in dynamic force microscopy. These results and technologies are now expanding the capacity of the NC-AFM with imaging functions on an atomic scale toward making them characterization and manipulation tools of individual atoms/molecules and nanostructures, with outstanding capability at the level of molecular, atomic, and subatomic resolution. Since the publication of vol. 2 of the book Noncontact Atomic Force Microscopy in 2009 the noncontact atomic force microscope, which can image even insulators with atomic resolution, has achieved remarkable progress. The NC-AFM is now becoming crucial for nanoscience and nanotechnology.

Handbook of Molecular Force Spectroscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 038749989X
Total Pages : 311 pages
Book Rating : 4.3/5 (874 download)

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Book Synopsis Handbook of Molecular Force Spectroscopy by : Aleksandr Noy

Download or read book Handbook of Molecular Force Spectroscopy written by Aleksandr Noy and published by Springer Science & Business Media. This book was released on 2007-12-03 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: Researchers in academia and industry who are interested in techniques for measuring intermolecular forces will find this an essential text. It presents a review of modern force spectroscopy, including fundamentals of intermolecular forces, technical aspects of the force measurements, and practical applications. The handbook begins with a review of the fundamental physics of loading single and multiple chemical bonds on the nanometer scale. It contains a discussion of thermodynamic and kinetic models of binding forces and dissipation effects in nanoscale molecular contacts, covers practical aspects of modern single-molecule level techniques, and concludes with applications of force spectroscopy to chemical and biological processes. Computer modeling of force spectroscopy experiments is also addressed.

Structure and Dynamics of Layered Molecular Assemblies

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ISBN 13 :
Total Pages : 400 pages
Book Rating : 4.3/5 (129 download)

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Book Synopsis Structure and Dynamics of Layered Molecular Assemblies by : Jennifer Conrad Horne

Download or read book Structure and Dynamics of Layered Molecular Assemblies written by Jennifer Conrad Horne and published by . This book was released on 1998 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Assembly in Natural and Engineered Systems

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Publisher : Academic Press
ISBN 13 : 0124159060
Total Pages : 427 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Molecular Assembly in Natural and Engineered Systems by : Stefan Howorka

Download or read book Molecular Assembly in Natural and Engineered Systems written by Stefan Howorka and published by Academic Press. This book was released on 2011-10-26 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores some of the most exciting recent advances in basic research on molecular assembly in natural and engineered systems and how this knowledge is leading to advances in the various fields. This series provides a forum for discussion of new discoveries, approaches, and idea Contributions from leading scholars and industry experts Reference guide for researchers involved in molecular biology and related fields

Molecular Scale Materials Assembly Investigated by Atomic Force Microscopy

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ISBN 13 :
Total Pages : 316 pages
Book Rating : 4.:/5 (713 download)

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Book Synopsis Molecular Scale Materials Assembly Investigated by Atomic Force Microscopy by : Kang Rae Cho

Download or read book Molecular Scale Materials Assembly Investigated by Atomic Force Microscopy written by Kang Rae Cho and published by . This book was released on 2010 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:

STM and AFM Studies on (Bio)molecular Systems: Unravelling the Nanoworld

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Publisher : Springer Science & Business Media
ISBN 13 : 3540783954
Total Pages : 326 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis STM and AFM Studies on (Bio)molecular Systems: Unravelling the Nanoworld by : Paolo Samori

Download or read book STM and AFM Studies on (Bio)molecular Systems: Unravelling the Nanoworld written by Paolo Samori and published by Springer Science & Business Media. This book was released on 2009-11-04 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Still valid and useful after a decade, this work presents critical reviews of the present position and future trends in modern chemical research. It contains short and concise reports on chemistry, each written by world-renowned experts.

Metal–Molecular Assembly for Functional Materials

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Publisher : Springer Science & Business Media
ISBN 13 : 4431543708
Total Pages : 94 pages
Book Rating : 4.4/5 (315 download)

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Book Synopsis Metal–Molecular Assembly for Functional Materials by : Yutaka Matsuo

Download or read book Metal–Molecular Assembly for Functional Materials written by Yutaka Matsuo and published by Springer Science & Business Media. This book was released on 2013-09-03 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on modern coordination chemistry, covering porous coordination polymers, metalloproteins, metallopeptides, nanoclusters, nanocapsules, aligned polymers, and fullerenes. As well, it deals with applications to electronic devices and surface characterization. These wide-ranging topics are integrally described from the perspectives of dimensionality (one-, two-, and three-dimension), new materials design, synthesis, molecular assembly, function and application. The nine chapters making up this book have been authored by scientists who are at the cutting edge of research in this particular field. The level is appropriate for graduate students, post-doc researchers, and new faculty members whose aim is to become familiar with modern coordination chemistry from its basics to applications.

Molecular Assembly of Biomimetic Systems

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Publisher : John Wiley & Sons
ISBN 13 : 3527634142
Total Pages : 288 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Molecular Assembly of Biomimetic Systems by : Junbai Li

Download or read book Molecular Assembly of Biomimetic Systems written by Junbai Li and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handy reference details state-of-the-art preparation of molecular assemblies of biotechnologically relevant biomimetic systems (artificial proteins, peptides, molecular motors, photosensitive systems) with an emphasis on biomimetic membranes, capsules, and interfaces. Medical applications such as drug release, gene therapy, and tissue engineering as well as biosensing, biocatalysis, and energy storage are highlighted.

The Handbook of Nanomedicine

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Publisher : Humana Press
ISBN 13 : 1493969668
Total Pages : 661 pages
Book Rating : 4.4/5 (939 download)

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Book Synopsis The Handbook of Nanomedicine by : Kewal K. Jain

Download or read book The Handbook of Nanomedicine written by Kewal K. Jain and published by Humana Press. This book was released on 2017-03-20 with total page 661 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomedicine is defined as the application of nanobiotechnology in clinical medicine, which is currently being used to research the pathomechanism of disease, refine molecular diagnostics, and aid in the discovery, development, and delivery of drugs. In The Handbook of Nanomedicine, Third Edition, Prof. Kewal K. Jain updates, reorganizes, and replaces information in the comprehensive second edition in order to capture the most recent advances in this dynamic field. Important components of nanomedicine such as drug delivery via nanobiotechnology and nanopharmaceuticals as well as nanooncology, where the greatest number of advances are occurring, are covered extensively. As this text is aimed at nonmedical scientists, pharmaceutical personnel, as well as physicians, descriptions of the technology involved and other medical terminology are kept as clear and simple as possible. In depth and cutting-edge, The Handbook of Nanomedicine, Third Edition informs its readers of the ever-growing field of nanomedicine, destined to play a significant role in the future of healthcare.

Biological Applications of the Atomic Force Microscope [microform] : Measuring the Forces that Hold DNA Together, And, Molecular Level, Direct, Real Time Observation of the Interactions of RecA Protein with Double Stranded DNA

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Publisher : Library and Archives Canada = Bibliothèque et Archives Canada
ISBN 13 : 9780612942622
Total Pages : 452 pages
Book Rating : 4.9/5 (426 download)

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Book Synopsis Biological Applications of the Atomic Force Microscope [microform] : Measuring the Forces that Hold DNA Together, And, Molecular Level, Direct, Real Time Observation of the Interactions of RecA Protein with Double Stranded DNA by : Bernard David Sattin

Download or read book Biological Applications of the Atomic Force Microscope [microform] : Measuring the Forces that Hold DNA Together, And, Molecular Level, Direct, Real Time Observation of the Interactions of RecA Protein with Double Stranded DNA written by Bernard David Sattin and published by Library and Archives Canada = Bibliothèque et Archives Canada. This book was released on 2004 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Employing the Atomic Force Microscope (AFM), two biological systems are examined under biological conditions. The force holding together single base pairs of DNA is probed. Measuring the force required to rupture a perfect double helix, a double helix with a single mismatch, and a double helix with a double mismatch, arranged in a microarray, the need for precise determination of the AFM tip spring constant is precluded. The large volume of data collected required the implementation of cluster analysis, used for the first time in single molecule force spectroscopy. The rupture force of the double helix ranged from 10 to 80 pN depending on the length of the double helix. Importantly, we found that the force contribution from both AT and GC base pairs is the same. RecA protein's interaction with double stranded DNA is studied using time resolved AFM. The RecA/DNA nucleofilament is formed under physiological conditions and imaged. Several different species are formed, and have a pitch of 10 nm. The assembly of the RecA/DNA complex is monitored by examining contour length change over time. The assembly pathway occurred via a slow nucleation step, followed by a rapid growth phase. The lag time was 25 minutes, and the growth time was 17 minutes. The maximum growth speed was 92 RecA per minute. The disassembly of the complex is monitored in real time under buffer at pH 7.4 and 5.6, at a time resolution on the order of 2 minutes, and a spatial resolution better then 50 nm. The complexes disassemble from multiple discrete nodes in a non-random manner. At pH 7.4 the disassembly is rapid (up to 39 monomers per minute per node), and two to four times slower (as slow as 4.5 monomers per minute per node) at pH 5.6. The design and implementation of a hot stage is described. The hot stage employs a simple, cost-effective design that allows a user to easily heat an AFM sample to 37 Celsius for experiments on biological systems. This is demonstrated by imaging DNA at ambient and physiological temperature.

Encyclopedia of Surface and Colloid Science

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Publisher : CRC Press
ISBN 13 : 9780849396083
Total Pages : 1152 pages
Book Rating : 4.3/5 (96 download)

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Book Synopsis Encyclopedia of Surface and Colloid Science by : P. Somasundaran

Download or read book Encyclopedia of Surface and Colloid Science written by P. Somasundaran and published by CRC Press. This book was released on 2006 with total page 1152 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Atomic Force Microscopy Investigations into Biology

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Publisher : BoD – Books on Demand
ISBN 13 : 9535101145
Total Pages : 374 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Atomic Force Microscopy Investigations into Biology by : Christopher Frewin

Download or read book Atomic Force Microscopy Investigations into Biology written by Christopher Frewin and published by BoD – Books on Demand. This book was released on 2012-03-07 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: The atomic force microscope (AFM) has become one of the leading nanoscale measurement techniques for materials science since its creation in the 1980's, but has been gaining popularity in a seemingly unrelated field of science: biology. The AFM naturally lends itself to investigating the topological surfaces of biological objects, from whole cells to protein particulates, and can also be used to determine physical properties such as Young's modulus, stiffness, molecular bond strength, surface friction, and many more. One of the most important reasons for the rise of biological AFM is that you can measure materials within a physiologically relevant environment (i.e. liquids). This book is a collection of works beginning with an introduction to the AFM along with techniques and methods of sample preparation. Then the book displays current research covering subjects ranging from nano-particulates, proteins, DNA, viruses, cellular structures, and the characterization of living cells.