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Author : Venkatesh Balan
Publisher : Methods in Molecular Biology
ISBN 13 : 9781493994861
Total Pages : 422 pages
Book Rating : 4.9/5 (948 download)
Download or read book Microbial Lipid Production: Methods and Protocols written by Venkatesh Balan and published by Methods in Molecular Biology. This book was released on 2020-06-14 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Terry J McGenity
Publisher : Springer
ISBN 13 : 9783662571149
Total Pages : 256 pages
Book Rating : 4.5/5 (711 download)
Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J McGenity and published by Springer. This book was released on 2018-12-30 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Author : Terry J. McGenity
Publisher :
ISBN 13 : 9783662491317
Total Pages : 204 pages
Book Rating : 4.4/5 (913 download)
Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by . This book was released on 2016 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662491386
Total Pages : 0 pages
Book Rating : 4.4/5 (913 download)
Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2016-11-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume features protocols for investigating the hydrocarbon- and lipid-specific activities of microbes. They include methods for studying chemotaxis, the colonisation of hydrocarbon surfaces, hydrocarbon uptake, respiration, nitrogen fixation, sulphate reduction, membrane stabilisation through cis-trans isomerisation of membrane fatty acids, and the production of biosurfactants and biopolymers in response to the presence of hydrocarbons. A protocol for studying the ability of microbes to control the concentration of hydrocarbons in their aqueous environment is also described, and phenotyping methods to reveal microbes’ more general metabolic activities are presented. Several protocols for investigating acid production in connection with oil souring and biocorrosion by microbes in oil well, oil transportation and storage settings are presented. Lastly, protocols for measuring methanogenesis, as an example of microbial hydrocarbon production, are described.“br>divHydrocarbon and Lipid Microbiology ProtocolsdivThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Author : Carlos Ricardo Soccol
Publisher : Elsevier
ISBN 13 : 0323998135
Total Pages : 378 pages
Book Rating : 4.3/5 (239 download)
Download or read book Biomass, Biofuels, Biochemicals written by Carlos Ricardo Soccol and published by Elsevier. This book was released on 2022-07-07 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial Lipids: Processes, Products, and Innovations brings together experienced authors on microbial lipids bioproduction, introducing the reader to key aspects from lipid chemistry and biochemistry to industrial production by fungi, yeast and microalgae. Intended for professionals in industry-oriented research and development, this volume also describes lipid production using synthetic media and agroindustry residues and discusses the potential of integration of microbial lipids in biorefineries, towards a circular economy. With a market of US$ 7 billion, oils and fats are one of the most important classes of natural products, with applications in food, feed, nutraceuticals, cosmetics, biofuel, and oleo chemistry. Primary sources include plants, animals and fish. However, specialty lipids such as oils rich in omega-3 and -6 fatty acids have limited sources and productivity. Microbial oils are gaining space in the global market as green – and even vegan – alternatives to traditional sources. Microorganisms also have high productivity and metabolic plasticity which makes it promising sources of new products. Includes key developments in lipids chemistry and biochemistry Provides technological developments on the production and applications of lipids from various microbial sources Covers details on non-conventional lipids Presents the latest advances on the downstream processing of lipids
Author : Terry J McGenity
Publisher : Springer
ISBN 13 : 9783662570814
Total Pages : 264 pages
Book Rating : 4.5/5 (78 download)
Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J McGenity and published by Springer. This book was released on 2019-01-12 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Nidhi Adlakha
Publisher : CRC Press
ISBN 13 : 1000468828
Total Pages : 398 pages
Book Rating : 4.0/5 (4 download)
Download or read book Biomass for Bioenergy and Biomaterials written by Nidhi Adlakha and published by CRC Press. This book was released on 2021-10-21 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomass for Bioenergy and Biomaterials presents an overview of recent studies developed specifically for lignocellulose-based production of biofuels, biochemicals, and functional materials. The emphasis is on using sustainable chemistry and engineering to develop innovative materials and fuels for practical applications. Technological strategies for the physical processing or biological conversion of biomass for material production are also presented. FEATURES Offers a comprehensive view of biomass processing, biofuel production, life cycle assessment, techno-economic analysis, and biochemical and biomaterial production Presents details of innovative strategies to pretreat biomass Helps readers understand the underlying metabolic pathways and identify the best engineering strategies for their native strain Highlights different strategies to make biomaterials from biomass Provides insight into the potential economic viability of the biomass-based process This book serves as an ideal reference for academic researchers and engineers working with renewable natural materials, the biorefining of lignocellulose, and biofuels. It can also be used as a comprehensive reference source for university students in metabolic, chemical, and environmental engineering.
Author : Terry J. McGenity
Publisher :
ISBN 13 : 9783662491379
Total Pages : 188 pages
Book Rating : 4.4/5 (913 download)
Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by . This book was released on 2016 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Author : Dorothea Bartels
Publisher :
ISBN 13 : 9781071613627
Total Pages : 472 pages
Book Rating : 4.6/5 (136 download)
Download or read book Plant Lipids written by Dorothea Bartels and published by . This book was released on 2021 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally.
Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662451786
Total Pages : 0 pages
Book Rating : 4.4/5 (517 download)
Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2017-06-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume describes methods for cultivating hydrocarbon-producing and -consuming microbes, covering compounds in a range of states – gaseous (e.g. methane), liquid (e.g. alkanes of intermediate molecular weight) and solid (e.g. many PAHs and asphaltene). It also examines the cultivation of aerobic and anaerobic hydrocarbon degraders using a range of electron acceptors (e.g. oxygen, nitrate, sulphate, metals, (per)chlorate), and a separate chapter is devoted to explaining the cultivation of methanogens. Special attention is given to: high-throughput cultivation, growing microbes as biofilms, and cultivating fastidious microbes, as well as the preservation of microbial pure cultures and consortia. Accordingly, this Volume will be of value to anyone embarking on the selective enrichment and cultivation of novel microorganisms. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Author : Maurine E. Linder
Publisher :
ISBN 13 : 9781493995325
Total Pages : 327 pages
Book Rating : 4.9/5 (953 download)
Download or read book Protein Lipidation written by Maurine E. Linder and published by . This book was released on 2019 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Lipidation: Methods and Protocols is a valuable resource for experts in the field and for investigators who encounter protein lipidation through their research on a particular cellular process or favorite protein.
Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662504499
Total Pages : 0 pages
Book Rating : 4.5/5 (44 download)
Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2017-01-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Author : Terry J McGenity
Publisher : Springer
ISBN 13 : 9783662570593
Total Pages : 270 pages
Book Rating : 4.5/5 (75 download)
Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J McGenity and published by Springer. This book was released on 2018-10-28 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume presents a comprehensive series of generic protocols for the genetic and genomic analysis of prokaryotic isolates. Genetic methods for functional analyses employ the latest cloning vectors, gene fusion methods and transposon mutagenesis systems, as well as systems for introducing protease-cleavage sequences into permissive sites in proteins under investigation. Genomic methods described include protocols for transcriptomics, shotgun proteomics, interactomics, metabolic profiling, and lipidomics. Bioinformatic tools for genome annotation, transcriptome display and the integration of transcriptomic data into genome-scale metabolic reconstructions are described. Protocols for 13C-based metabolic flux determinations and analysis of the hierarchical and metabolic regulation of fluxes through pathways are included. The Volume thus enables investigators to functionally analyse an isolate over the entire cellular range spanning the gene, the genome, the transcript repertoire, the proteome, the interactome, the metabolic network with its nodes and their regulatory hierarchies, and the metabolic fluxes and their physiological controls. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Author : Peter Sass
Publisher : Springer Nature
ISBN 13 : 1071628550
Total Pages : 407 pages
Book Rating : 4.0/5 (716 download)
Download or read book Antibiotics written by Peter Sass and published by Springer Nature. This book was released on 2022-11-29 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols, Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research.
Author : Abu Yousuf
Publisher : CRC Press
ISBN 13 : 1000851222
Total Pages : 331 pages
Book Rating : 4.0/5 (8 download)
Download or read book Microbiology of Green Fuels written by Abu Yousuf and published by CRC Press. This book was released on 2023-03-10 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: The replacement of fossil-derived compounds by bio-based fuels and chemicals is crucial for the implementation of a sustainable bioeconomy. In this context, microorganisms are key players for biofuels’ production from renewable sources. Biotechnological biofuel production processes require conversion microorganisms capable of both efficiently assimilating renewable low-cost carbon sources and diverting their metabolisms towards the specific biofuel. Exploring the wide diversity of microorganisms available on Earth will surely aid to make the production of green fuels a reality. This book gives a wide overview of different microbial-based processes for green fuels production. The book also includes techno-economic analysis and highlights strategic, commercial and environmental interests in promoting green fuels. All these facts make this book very valuable not only for the scientific community but also for biofuel companies and policy makers.
Author : Junping Peng
Publisher : Frontiers Media SA
ISBN 13 : 288974454X
Total Pages : 169 pages
Book Rating : 4.8/5 (897 download)
Download or read book Progress in Pathogen Identification Based on Mass Spectrometry written by Junping Peng and published by Frontiers Media SA. This book was released on 2022-02-21 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Travis R. Glare
Publisher : Humana
ISBN 13 : 9781493981786
Total Pages : 224 pages
Book Rating : 4.9/5 (817 download)
Download or read book Microbial-Based Biopesticides written by Travis R. Glare and published by Humana. This book was released on 2018-06-20 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on the developmental areas of biopesticides: production, formulation, application and field efficacy. Chapters guide readers through methods and techniques on environmental, mammalian, safety, and registration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microbial-Based Biopesticides aims to ensure successful results in the further study of this vital field.
