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Author : Gary C. Howard
Publisher : McGraw-Hill/Appleton & Lange
ISBN 13 : 9780838569467
Total Pages : 342 pages
Book Rating : 4.5/5 (694 download)
Download or read book Methods in Nonradioactive Detection written by Gary C. Howard and published by McGraw-Hill/Appleton & Lange. This book was released on 1993-01-01 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Elena Hilario
Publisher : Springer Science & Business Media
ISBN 13 : 1597452297
Total Pages : 318 pages
Book Rating : 4.5/5 (974 download)
Download or read book Protocols for Nucleic Acid Analysis by Nonradioactive Probes written by Elena Hilario and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author : T. Takahashi
Publisher :
ISBN 13 :
Total Pages : 7 pages
Book Rating : 4.:/5 ( download)
Download or read book A Nonradioactive Nucleic Acid Labeling Method and a Detection Method Using it written by T. Takahashi and published by . This book was released on 1989 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Christoph Kessler
Publisher : Springer Science & Business Media
ISBN 13 : 9783540646013
Total Pages : 1170 pages
Book Rating : 4.6/5 (46 download)
Download or read book Nonradioactive Analysis of Biomolecules written by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2000-03-11 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.
Author : Christoph Kessler
Publisher : Springer
ISBN 13 : 3662001446
Total Pages : 449 pages
Book Rating : 4.6/5 (62 download)
Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer. This book was released on 2012-12-06 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Author : David William Johnson
Publisher :
ISBN 13 :
Total Pages : 176 pages
Book Rating : 4.:/5 (256 download)
Download or read book Quick Blots and Nonradioactive Detection Systems written by David William Johnson and published by . This book was released on 1990 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Tim Meads
Publisher :
ISBN 13 :
Total Pages : 82 pages
Book Rating : 4.:/5 (637 download)
Download or read book DNA Fingerprinting and the Use of Non Radioactive Detection Methods written by Tim Meads and published by . This book was released on 1988 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : A. Khoo
Publisher :
ISBN 13 :
Total Pages : 125 pages
Book Rating : 4.:/5 (969 download)
Download or read book Non-radioactive Detection System for PCR-microsatellite Length written by A. Khoo and published by . This book was released on 1994 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detection was carried out using a streptavidin-alkaline phosphate system using a chemiliminescent method. The results were read by observing the bands on the autoradiograph. This nonradioactive method for microsatellite-length polymorphism is an alternative to the more commonly use radioactive method. [Authors' abstract].
Author : Gabriel Bela Kalmar
Publisher :
ISBN 13 :
Total Pages : 180 pages
Book Rating : 4.:/5 (22 download)
Download or read book A Non-radioactive Method of Detecting Nucleic Acids written by Gabriel Bela Kalmar and published by . This book was released on 1986 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : A. J. Garman
Publisher : Elsevier
ISBN 13 : 0080551092
Total Pages : 155 pages
Book Rating : 4.0/5 (85 download)
Download or read book Non-Radioactive Labelling written by A. J. Garman and published by Elsevier. This book was released on 1997-03-20 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules. Aimed at researchers without specific expertise in chemistry, the book includes: A review of the main signal systems and labels available, indicating their strengths and weaknesses Discussion of the most useful strategies for labelling the various biomolecules 32 protocols covering common labelling needs Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications Sources of information including references, data and suppliers
Author : Larry J. Kricka
Publisher : Academic Press
ISBN 13 : 0323137717
Total Pages : 373 pages
Book Rating : 4.3/5 (231 download)
Download or read book Nonisotopic DNA Probe Techniques written by Larry J. Kricka and published by Academic Press. This book was released on 2012-12-02 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided. This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection. Gives extensive practical information Covers major types of nonisotopic labels and procedures Presents background information for each method Provides strategies and detailed experimental procedures for labeling and detecting DNA sequences by Fluorescence Chemiluminescence Bioluminescence Colorimetry
Author : Suat Yilmaz
Publisher :
ISBN 13 :
Total Pages : 306 pages
Book Rating : 4.:/5 (449 download)
Download or read book Development of Non-radioactive Detection Methods for the Genomic RNAs of Ilarviruses that Cause Disease in Peach and Their Use in Studying the Molecular Basis of the Synergistic Interaction Between Prunus Necrotic Ringspot Virus and Prune Dwarf Virus written by Suat Yilmaz and published by . This book was released on 1999 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Graham R. Taylor
Publisher : CRC Press
ISBN 13 : 9780849392337
Total Pages : 360 pages
Book Rating : 4.3/5 (923 download)
Download or read book Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA written by Graham R. Taylor and published by CRC Press. This book was released on 1997-01-24 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: History and background Principles and theory Equipment and reagents Protocols Troubleshooting Applications Improvements Results Comparisons with other methods Future prospects and developments This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.
Author : P. Ruback
Publisher :
ISBN 13 :
Total Pages : 17 pages
Book Rating : 4.:/5 ( download)
Download or read book Method of Coloring Nucleic Acid Detected by Nonradioactive Labelling and Reactants for Conducting Said Method written by P. Ruback and published by . This book was released on 1990 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Larry J. Kricka
Publisher : Elsevier
ISBN 13 : 0080537669
Total Pages : 539 pages
Book Rating : 4.0/5 (85 download)
Download or read book Nonisotopic Probing, Blotting, and Sequencing written by Larry J. Kricka and published by Elsevier. This book was released on 1995-06-01 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules Gives protocols for nonisotopic DNA sequencing - new in this edition Gives extensive, practical information Presents background information for each method Provides expert accounts from the inventor or developer of each method Contains seven entirely new chapters Covers all major types of nonisotopic procedures for labeling and detection
Author : Thomas Köhler
Publisher : Springer
ISBN 13 : 9783642797149
Total Pages : 0 pages
Book Rating : 4.7/5 (971 download)
Download or read book Quantitation of mRNA by Polymerase Chain Reaction written by Thomas Köhler and published by Springer. This book was released on 2011-12-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
Author : Christoph Kessler
Publisher : Springer Verlag
ISBN 13 : 9780387554822
Total Pages : 436 pages
Book Rating : 4.5/5 (548 download)
Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer Verlag. This book was released on 1992-01-01 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt:
