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Methods In Membrane Lipids
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Book Synopsis Methods in Membrane Lipids by : Alex M. Dopico
Download or read book Methods in Membrane Lipids written by Alex M. Dopico and published by Springer Science & Business Media. This book was released on 2007-08-30 with total page 1265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. The volume provides a comprehensive set of techniques for studying membrane lipids with a strong biophysical emphasis. It compares the various available techniques including the pros and cons as seen by the experts.
Book Synopsis Membrane Lipids by : Charles G. Cranfield
Download or read book Membrane Lipids written by Charles G. Cranfield and published by Humana. This book was released on 2021-12-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.
Book Synopsis Methods in Membrane Lipids by : Dylan M. Owen
Download or read book Methods in Membrane Lipids written by Dylan M. Owen and published by Humana. This book was released on 2016-09-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico. Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.
Book Synopsis An Introduction to Biological Membranes by : William Stillwell
Download or read book An Introduction to Biological Membranes written by William Stillwell and published by Newnes. This book was released on 2013-04-20 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. Brings together different facets of membrane research in a universally understandable manner Emphasis on the historical development of the field Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport.
Book Synopsis Molecular Biology of The Cell by : Bruce Alberts
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Membrane Biogenesis by : Doron Rapaport
Download or read book Membrane Biogenesis written by Doron Rapaport and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques. In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
Book Synopsis Biochemistry of Lipids, Lipoproteins and Membranes by : Neale Ridgway
Download or read book Biochemistry of Lipids, Lipoproteins and Membranes written by Neale Ridgway and published by Elsevier. This book was released on 2015-07-24 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry of Lipids: Lipoproteins and Membranes, Volume Six, contains concise chapters that cover a wide spectrum of topics in the field of lipid biochemistry and cell biology. It provides an important bridge between broad-based biochemistry textbooks and more technical research publications, offering cohesive, foundational information. It is a valuable tool for advanced graduate students and researchers who are interested in exploring lipid biology in more detail, and includes overviews of lipid biology in both prokaryotes and eukaryotes, while also providing fundamental background on the subsequent descriptions of fatty acid synthesis, desaturation and elongation, and the pathways that lead the synthesis of complex phospholipids, sphingolipids, and their structural variants. Also covered are sections on how bioactive lipids are involved in cell signaling with an emphasis on disease implications and pathological consequences. Serves as a general reference book for scientists studying lipids, lipoproteins and membranes and as an advanced and up-to-date textbook for teachers and students who are familiar with the basic concepts of lipid biochemistry References from current literature will be included in each chapter to facilitate more in-depth study Key concepts are supported by figures and models to improve reader understanding Chapters provide historical perspective and current analysis of each topic
Book Synopsis Methods in Membrane Biology by : Edward D. Korn
Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although not the only volume in this series in which lipids are discussed, the present volume is devoted entirely to methods for the study of membrane lipids. Even now, when membrane proteins are properly receiving so much attention, this emphasis on membrane lipids is appropriate. Essentially all of the phospholipids and sterols of cells are in membranes. Moreover, although membrane proteins are certainly of utmost importance, the more we learn about the functional properties of membrane proteins, the more we appreciate the unique features of phospholipids, without which biological membranes would be impossible. The hydrophobic-hydrophilic duality of phospholipids allows, indeed requires, their association, in an aqueous environment, into an essentially two-dimensional membrane-only molec ularly thick in one dimension but relatively infinite in the other two; a structure composed of small molecules, not covalently linked, and therefore, infinitely mobile and variable, but yet a structure with great stability and one largely impermeable to most biomolecules. These membrane-forming properties are shared by many amphipathic polar lipids-phospholipids, glycolipids, and sphingolipids-that differ significantly from each other in the nature of their polar head groups and their fatty acids. These variations in structure allow a range of specific interactions among membrane lipids and between lipids and proteins and also provide for membranes of variable, but controlled, fluidity. In this way, phospholipids provide an appropriate milieu for functional membrane proteins and also significantly modulate their catalytic activities.
Book Synopsis Membrane Lipids by : Charles G. Cranfield
Download or read book Membrane Lipids written by Charles G. Cranfield and published by Humana. This book was released on 2021-12-02 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.
Book Synopsis New Methods and Sensors for Membrane and Cell Volume Research by :
Download or read book New Methods and Sensors for Membrane and Cell Volume Research written by and published by Academic Press. This book was released on 2021-12-01 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest. Describes novel FRET-based membrane sensors Reviews selected non-optical approaches to membrane structure and dynamics Describes traditional and modern aspects of cell volume research, such as phase transitions and macromolecular crowding
Book Synopsis Methods in Membrane Biology by : Edward D. Korn
Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer. This book was released on 1979-01-04 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three articles make up Volume 10 of Methods in Membrane Biology. In the first of these, Papahadjopoulos, Poste, and Vail extensively review much of the available data on the fusion of natural membranes, model membranes (liposomes), and natural membranes with liposomes. The authors are led by their review of the experimental methods and their interpretations of the results obtained to a general theory of membrane fusion which they believe is applicable to all systems that have been studied. Arguing that although protein and carbohydrate may serve, in some cases, to bring membranes into sufficiently close proximity for fusion to occur and, in other cases, to remove peripheral and integral proteins from the regions that are to undergo fusion, the authors conclude that membrane fusion per se is solely a property of the lipid bilayer. In their view, all the experimental observations to date can be subsumed under a unifying hypothesis in which membrane fusion is the result of a phase separation in one-half of the membrane bilayer brought about by the interaction - of calcium ions with acidic phospholipids, mostly phosphatidylserine. Where half-membranes already contain sufficient acidic phospholipids, a local increase in calcium ion concentration may suffice to induce fusion (examples might include exocytosis and fusion of intracellular membrane systems). In other cases, natural or experimentally induced events preceding fusion might be necessary to increase the local concentration of the acidic phospholipids in the half-membrane (virus-or fusogenic agent-induced cell-to-cell fusion, or endocytosis, for example).
Book Synopsis Handbook of Lipid Membranes by : Cyrus R. Safinya
Download or read book Handbook of Lipid Membranes written by Cyrus R. Safinya and published by CRC Press. This book was released on 2021-09-14 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook provides a unique overview of lipid membrane fundamentals and applications. The fascinating world of lipids that harbor and govern so many biological functionalities are discussed within the context of membrane structures, interactions, and shape evolution. Beyond the fundamentals in lipid science, this handbook focuses on how scientists are building bioinspired biomimetic systems for applications in medicine, cosmetics, and nanotechnology. Key Features: Includes experimental and theoretical overviews on the role of lipids, with or without associated biomolecules, as structural components imparting distinct membrane shapes and intermembrane interactions Covers the mechanisms of lipid-membrane curvature, by peptide and protein binding, and the roles of signalling lipids and the cytoskeleton in plasma membrane shape evolution Covers advanced X-ray and force measurement techniques Discusses applications in biomedicine, cosmetics, and nanotechnology, including lipid vectors in nucleic acid, drug delivery in dermal applications, and lipid-based sensors and artificial biointerfaces Covers artificial membranes from block copolymers, synthetic copolypeptides, and recombinant proteins Includes an exciting section that explores the role of lipids in the origin of life in hydrothermal conditions This book is a highly informative companion for professionals in biophysics, biochemistry, physical chemistry, and material and pharmaceutical sciences and bioengineering.
Book Synopsis Planar Lipid Bilayers by : Wolfgang Hanke
Download or read book Planar Lipid Bilayers written by Wolfgang Hanke and published by . This book was released on 1993 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. Methods of constructing artificial membranes (planar lipid bilayers) from the main components of cell membranes (lipids) date from the early 1960s. Planar bilayers offer direct, quantitative experimental approaches to the study of membranes of precisely determined composition which can be manipulated by the experimenter. Pore-forming molecules, transporter molecules, ATP-dependent enzymes and other entities can be incorporated into the bilayers to simulate biological functions. Reconstitution of such functions in this way remains a key final step in attributing a functional role to purified cell membrane proteins. This book aims to demystify these techniques and begins with a broad overview of the development of the subject before dealing with the protocols involved. Key references are provided at the end of the book together with a list of suppliers. Full practical details include: * How to set up conventional painted and folded bilayer experiments * Patch-dipping methods and use of giant liposomes * Lipid characterization, preparation and purification * Discussion of construction of essential apparatus, flux measurements, electrical recording, data acquisition and computer support * Biochemical methods for use in planar bilayer experiments * Techniques for incorporation of native proteins and other molecules
Book Synopsis Methods in Membrane Biology by : Edward D. Korn
Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purposes of this senes were discussed in the preface to Volume I: to present "a range of methods . . . from the physical to the physiological . . . in sufficient detail for the reader to use them in his laboratory" and also to describe "the theoretical backgrounds of the methods and their limita tions in membrane biology" so that the reader will be enabled "to evaluate more critically and to understand more fully data obtained by methods foreign to [his] usual experiences. " The chapter by Lee, Birdsall, and Metcalfe with which Volume 2 begins accomplishes these twin goals with a thorough description of the application of nuclear magnetic relaxation measurements to membrane biology together with a lucid and succinct integration of the results of such studies into present concepts of the organi zation of membrane lipids. This then permits speculation on the physical basis of membrane permeability. The powerful tool of NMR spectroscopy will have even fuller application with the development of techniques, al ready partially exploited, for l3C-Iabeling of specific carbon atoms in lipid molecules and with extension of the observations to membrane proteins. The following two chapters, by Glick and by Laine, Stellner, and Hako mori, describe the isolation and characterization of membrane glycoproteins and membrane glycolipids, respectively.
Book Synopsis Manual on Membrane Lipids by : Rajendra Prasad
Download or read book Manual on Membrane Lipids written by Rajendra Prasad and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although previously thought to be merely passive structural components, membrane lipids have recently been found to be actively involved in cellular transport and signal transduction processes. Clear protocols for the study of membrane lipid properties, cellular transport or signal transduction are presented in this manual. Following a short introduction to membrane lipids, techniques for the isolation and extraction of membrane fractions, the analysis of the lipid composition, lipid turnover, and the involvement in signal transduction as well as the preparation of liposomes are described.
Book Synopsis Cell Biology by the Numbers by : Ron Milo
Download or read book Cell Biology by the Numbers written by Ron Milo and published by Garland Science. This book was released on 2015-12-07 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Book Synopsis Plant Lipid Metabolism by : J.C. Kader
Download or read book Plant Lipid Metabolism written by J.C. Kader and published by Springer Science & Business Media. This book was released on 2013-04-18 with total page 598 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of papers that comprehensively describe the major areas of research on lipid metabolism of plants. State-of-the-art knowledge about research on fatty acid and glycerolipid biosynthesis, isoprenoid metabolism, membrane structure and organization, lipid oxidation and degradation, lipids as intracellular and extracellular messengers, lipids and environment, oil seeds and gene technology is reviewed. The different topics covered show that modern tools of plant cellular and molecular biology, as well as molecular genetics, have been recently used to characterize several key enzymes of plant lipid metabolism (in particular, desaturases, thioesterases, fatty acid synthetase) and to isolate corresponding cDNAs and genomic clones, allowing the use of genetic engineering methods to modify the composition of membranes or storage lipids. These findings open fascinating perspectives, both for establishing the roles of lipids in membrane function and intracellular signalling and for adapting the composition of seed oil to the industrial needs. This book will be a good reference source for research scientists, advanced students and industrialists wishing to follow the considerable progress made in recent years on plant lipid metabolism and to envision the new opportunities offered by genetic engineering for the development of novel oil seeds.
