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Membrane Fusion Techniques
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Book Synopsis Membrane Fusion Techniques by : Nejat Düzgüneş
Download or read book Membrane Fusion Techniques written by Nejat Düzgüneş and published by Gulf Professional Publishing. This book was released on 1993 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. * * Major topics covered include: * Cell-cell fusion mediated by viruses and viral proteins * Conformational changes of proteins during membrane fusion * Membrane fusion during exocytosis * Intracellular membrane fusion * Membrane fusion in fertilization * Introduction of macromolecules into cells by membrane fusion * Protoplast fusion.
Book Synopsis The Giant Vesicle Book by : Rumiana Dimova
Download or read book The Giant Vesicle Book written by Rumiana Dimova and published by CRC Press. This book was released on 2019-11-19 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt: Giant vesicles are widely used as a model membrane system, both for basic biological systems and for their promising applications in the development of smart materials and cell mimetics, as well as in driving new technologies in synthetic biology and for the cosmetics and pharmaceutical industry. The reader is guided to use giant vesicles, from the formation of simple membrane platforms to advanced membrane and cell system models. It also includes fundamentals for understanding lipid or polymer membrane structure, properties and behavior. Every chapter includes ideas for further applications and discussions on the implications of the observed phenomena towards understanding membrane-related processes. The Giant Vesicle Book is meant to be a road companion, a trusted guide for those making their first steps in this field as well as a source of information required by experts. Key Features • A complete summary of the field, covering fundamental concepts, practical methods, core theory, and the most promising applications • A start-up package of theoretical and experimental information for newcomers in the field • Extensive protocols for establishing the required preparations and assays • Tips and instructions for carefully performing and interpreting measurements with giant vesicles or for observing them, including pitfalls • Approaches developed for investigating giant vesicles as well as brief overviews of previous studies implementing the described techniques • Handy tables with data and structures for ready reference
Book Synopsis The Membranes of Cells by : Philip Yeagle
Download or read book The Membranes of Cells written by Philip Yeagle and published by . This book was released on 1993 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition of The Membranes of Cells, all of the chapters have been updated, some have been completely rewritten, and a new chapter on receptors has been added. The book has been designed to provide both the student and researcher with a synthesis of information from a number of scientific disciplines to create a comprehensive view of the structure and function of the membranes of cells. The topics are treated in sufficient depth to provide an entry point to the more detailed literature needed by the researcher. Key Features * Introduces biologists to membrane structure and physical chemistry * Introduces biophysicists to biological membrane function * Provides a comprehensive view of cell membranes to students, either as a necessary background for other specialized disciplines or as an entry into the field of biological membrane research * Clarifies ambiguities in the field
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structure and Dynamics of Membranes by : R. Lipowsky
Download or read book Structure and Dynamics of Membranes written by R. Lipowsky and published by Elsevier. This book was released on 1995-06-15 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first volume of the Handbook deals with the amazing world of biomembranes and lipid bilayers. Part A describes all aspects related to the morphology of these membranes, beginning with the complex architecture of biomembranes, continues with a description of the bizarre morphology of lipid bilayers and concludes with technological applications of these membranes. The first two chapters deal with biomembranes, providing an introduction to the membranes of eucaryotes and a description of the evolution of membranes. The following chapters are concerned with different aspects of lipids including the physical properties of model membranes composed of lipid-protein mixtures, lateralphase separation of lipids and proteins and measurement of lipid-protein bilayer diffusion. Other chapters deal with the flexibility of fluid bilayers, the closure of bilayers into vesicles which attain a large variety of different shapes, and applications of lipid vesicles and liposomes. Part B covers membrane adhesion, membrane fusion and the interaction of biomembranes withpolymer networks such as the cytoskeleton. The first two chapters of this part discuss the generic interactions of membranes from the conceptual point of view. The following two chapters summarize the experimental work on two different bilayer systems. The next chapter deals with the process ofcontact formation, focal bounding and macroscopic contacts between cells. The cytoskeleton within eucaryotic cells consists of a network of relatively stiff filaments of which three different types of filaments have been identified. As explained in the next chapter much has been recently learned aboutthe interaction of these filaments with the cell membrane. The final two chapters deal with membrane fusion.
Book Synopsis Neurotransmitter Release by : Hugo J. Bellen
Download or read book Neurotransmitter Release written by Hugo J. Bellen and published by Oxford University Press, USA. This book was released on 1999 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the reader with background information on neurotransmitter release. Emphasis is placed on the rationale by which proteins are assigned specific functions rather than just providing facts about function.
Download or read book Membrane Fusion written by Jan Wilschut and published by CRC Press. This book was released on 2019-11-14 with total page 927 pages. Available in PDF, EPUB and Kindle. Book excerpt: This balanced volume provides a broad and coherent overview of recent progress in membrane fusion research—highlighting an interdisciplinary treatment of the subject from the fields of biophysics, biochemistry, cell biology, virology, and biotechnology—in a single volume., Featuring easy-access sections on the general properties of membranes and applications of membrane fusion techniques, this valuable sourcebook outlines membrane structure, lipid polymorphism, and intermembrane forces ... covers membrane fusion in model systems ... presents the fusogenic properties of enveloped viruses ... discusses the fusion and flow of intracellular membranes and cell-cell fusion occurring during fertilization and myogenesis ... offers applications of membrane fusion techniques in cell-biological research and biotechnology ... and more. Supplying a comprehensive view of this exciting topic, Membrane Fusion is a working resource for molecular, cell, and membrane biologists; biophysicists; biochemists; virologists; biotechnologists; microbiologists; immunologists; physiologists; and graduate and medical school students in biophysics, biochemistry, physiology, virology, cell biology, and biotechnology.
Book Synopsis Principles of Cloning by : Jose Cibelli
Download or read book Principles of Cloning written by Jose Cibelli and published by Academic Press. This book was released on 2013-09-24 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species—including mice, sheep, cattle, and non-mammals—is considered as well. The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine – Dr John Gurdon; the cloning of the first mammal from a somatic cell – Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual – Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell – Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas. - First and most comprehensive book on animal cloning, 100% revised - Describes an in-depth analysis of current limitations of the technology and research areas to explore - Offers cloning applications on basic biology, agriculture, biotechnology, and medicine
Book Synopsis Fusion Protein Technologies for Biopharmaceuticals by : Stefan R. Schmidt
Download or read book Fusion Protein Technologies for Biopharmaceuticals written by Stefan R. Schmidt and published by John Wiley & Sons. This book was released on 2013-01-28 with total page 995 pages. Available in PDF, EPUB and Kindle. Book excerpt: The state of the art in biopharmaceutical FUSION PROTEIN DESIGN Fusion proteins belong to the most lucrative biotech drugs—with Enbrel® being one of the best-selling biologics worldwide. Enbrel® represents a milestone of modern therapies just as Humulin®, the first therapeutic recombinant protein for human use, approved by the FDA in 1982 and Orthoclone® the first monoclonal antibody reaching the market in 1986. These first generation molecules were soon followed by a plethora of recombinant copies of natural human proteins, and in 1998, the first de novo designed fusion protein was launched. Fusion Protein Technologies for Biopharmaceuticals examines the state of the art in developing fusion proteins for biopharmaceuticals, shedding light on the immense potential inherent in fusion protein design and functionality. A wide pantheon of international scientists and researchers deliver a comprehensive and complete overview of therapeutic fusion proteins, combining the success stories of marketed drugs with the dynamic preclinical and clinical research into novel drugs designed for as yet unmet medical needs. The book covers the major types of fusion proteins—receptor-traps, immunotoxins, Fc-fusions and peptibodies—while also detailing the approaches for developing, delivering, and improving the stability of fusion proteins. The main body of the book contains three large sections that address issues key to this specialty: strategies for extending the plasma half life, the design of toxic proteins, and utilizing fusion proteins for ultra specific targeting. The book concludes with novel concepts in this field, including examples of highly relevant multifunctional antibodies. Detailing the innovative science, commercial realities, and brilliant potential of fusion protein therapeutics, Fusion Protein Technologies for Biopharmaceuticals is a must for pharmaceutical scientists, biochemists, medicinal chemists, molecular biologists, pharmacologists, and genetic engineers interested in determining the shape of innovation in the world of biopharmaceuticals.
Book Synopsis Membrane Biogenesis by : Doron Rapaport
Download or read book Membrane Biogenesis written by Doron Rapaport and published by Humana. This book was released on 2013-09-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques. In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
Book Synopsis Biological Soft Matter by : Corinne Nardin
Download or read book Biological Soft Matter written by Corinne Nardin and published by John Wiley & Sons. This book was released on 2021-04-06 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Soft Matter Explore a comprehensive, one-stop reference on biological soft matter written and edited by leading voices in the field Biological Soft Matter: Fundamentals, Properties and Applications delivers a unique and indispensable compilation of up-to-date knowledge and material on biological soft matter. The book presents a thorough overview about biological soft matter, beginning with different substance classes, including proteins, nucleic acids, lipids, and polysaccharides. It goes on to describe a variety of superstructures and aggregated and how they are formed by self-assembly processes like protein folding or crystallization. The distinguished editors have included materials with a special emphasis on macromolecular assembly, including how it applies to lipid membranes, and proteins fibrillization. Biological Soft Matter is a crucial resource for anyone working in the field, compiling information about all important substance classes and their respective roles in forming superstructures. The book is ideal for beginners and experts alike and makes the perfect guide for chemists, physicists, and life scientists with an interest in the area. Readers will also benefit from the inclusion of: An introduction to DNA nano-engineering and DNA-driven nanoparticle assembly Explorations of polysaccharides and glycoproteins, engineered biopolymers, and engineered hydrogels Discussions of macromolecular assemblies, including liquid membranes and small molecule inhibitors for amyloid aggregation A treatment of inorganic nanomaterials as promoters and inhibitors of amyloid fibril formation An examination of a wide variety of natural and artificial polymers Perfect for materials scientists, biochemists, polymer chemists, and protein chemists, Biological Soft Matter: Fundamentals, Properties and Applications will also earn a place in the libraries of biophysicists and physical chemists seeking a one-stop reference summarizing the rapidly evolving topic of biological soft matter.
Book Synopsis Physics of Biological Membranes by : Patricia Bassereau
Download or read book Physics of Biological Membranes written by Patricia Bassereau and published by Springer. This book was released on 2018-12-30 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book mainly focuses on key aspects of biomembranes that have emerged over the past 15 years. It covers static and dynamic descriptions, as well as modeling for membrane organization and shape at the local and global (at the cell level) scale. It also discusses several new developments in non-equilibrium aspects that have not yet been covered elsewhere. Biological membranes are the seat of interactions between cells and the rest of the world, and internally, they are at the core of complex dynamic reorganizations and chemical reactions. Despite the long tradition of membrane research in biophysics, the physics of cell membranes as well as of biomimetic or synthetic membranes is a rapidly developing field. Though successful books have already been published on this topic over the past decades, none include the most recent advances. Additionally, in this domain, the traditional distinction between biological and physical approaches tends to blur. This book gathers the most recent advances in this area, and will benefit biologists and physicists alike.
Book Synopsis Membrane Fusion by : Leonid V. Chernomordik
Download or read book Membrane Fusion written by Leonid V. Chernomordik and published by Academic Press. This book was released on 2011-07-27 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Current Topics in Membranes focuses on Membrane Fusion, beginning with fusion and fission of lipid bilayers, with reviews focused on hemifusion and dynamic remodeling of membranes catalyzed by dynamin. Other topics discussed include viral fusion, intracellular fusion, developmental cell fusion, and theoretical modeling.
Book Synopsis Sexual Reproduction in Animals and Plants by : Hitoshi Sawada
Download or read book Sexual Reproduction in Animals and Plants written by Hitoshi Sawada and published by Springer. This book was released on 2014-02-07 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains the proceedings of the International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants, where many plant and animal reproductive biologists gathered to discuss their recent progress in investigating the shared mechanisms and factors involved in sexual reproduction. This now is the first book that reviews recent progress in almost all fields of plant and animal fertilization. It was recently reported that the self-sterile mechanism of a hermaphroditic marine invertebrate (ascidian) is very similar to the self-incompatibility system in flowering plants. It was also found that a male factor expressed in the sperm cells of flowering plants is involved in gamete fusion not only of plants but also of animals and parasites. These discoveries have led to the consideration that the core mechanisms or factors involved in sexual reproduction may be shared by animals, plants and unicellular organisms. This valuable book is highly useful for reproductive biologists as well as for biological scientists outside this field in understanding the current progress of reproductive biology.
Book Synopsis Molecular and Diagnostic Procedures in Mycoplasmology by :
Download or read book Molecular and Diagnostic Procedures in Mycoplasmology written by and published by Academic Press. This book was released on 1996-02-21 with total page 489 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. - Diagnostic genetic probes - Immunological tools - Antibiotic sensitivity testing - Diagnosis of specific diseases - Experimental infections - Diagnosis of mycoplasma infections of cell cultures
Book Synopsis Guide to Electroporation and Electrofusion by : Donald C. Chang
Download or read book Guide to Electroporation and Electrofusion written by Donald C. Chang and published by Academic Press. This book was released on 2012-12-02 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. - Covers fundamentals of electroporation and electrofusion in detail: Molecular events, Mechanisms, Kinetics, Gives extensive practical information, The latest applications, Controlling parameters to maximize efficiency, Available instrumentation - Presents applications of electroporation and electrofusion in current research situations - State-of-the-art modifications to electrical pulses and generators - Application of electroporation and electrofusion to unique, alternative cell and tissue types - Gives straightforward, detailed, easy-to-follow protocols for Formation of human hybridomas - Introduction of genetic material into plant cells and pollen - Transfection of mammalian cells - Transformation of bacteria, plants, and yeast - Production of altered embryos - Optimization of electroporation by using reporter genes - Comprehensive and up-to-date - Convenient bench-top format - Approximately 125 illustrations complement the text - Complete references with article titles - Written by leading authorities in electroporation and electrofusion
Book Synopsis Computational Biophysics of Membrane Proteins by : Carmen Domene
Download or read book Computational Biophysics of Membrane Proteins written by Carmen Domene and published by Royal Society of Chemistry. This book was released on 2016-11-30 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: Exploring current themes in modern computational and membrane protein biophysics, this book presents a comprehensive account of the fundamental principles underlying different methods and techniques used to describe the intriguing mechanisms by which membrane proteins function. The book discusses the experimental approaches employed to study these proteins, with chapters reviewing recent crucial structural advances that have allowed computational biophysicists to discern how these molecular machines work. The book then explores what computational methods are available to researchers and what these have taught us about three key families of membrane proteins: ion channels, transporters and receptors. The book is ideal for researchers in computational chemistry and computational biophysics.
