Mechanisms Of Condition Specific Regulation Of Mrna Stability By Puf Proteins

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Mechanisms of Condition-Specific Regulation of MRNA Stability by Puf Proteins

Author : Joseph Russo (Ph.D.)
Publisher :
ISBN 13 :
Total Pages : 182 pages
Book Rating : 4.:/5 (113 download)

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Book Synopsis Mechanisms of Condition-Specific Regulation of MRNA Stability by Puf Proteins by : Joseph Russo (Ph.D.)

Download or read book Mechanisms of Condition-Specific Regulation of MRNA Stability by Puf Proteins written by Joseph Russo (Ph.D.) and published by . This book was released on 2014 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA binding proteins regulate mRNA decay and translation, two key steps in the control of gene expression in cells. Controlling mRNA metabolism allows cells to respond rapidly to altering conditions by utilizing already available mRNA, bypassing the wait for newly transcribed mRNA. The Puf family of RNA binding proteins bind specific mRNAs through interactions with sequences located in the 3’ untranslated region (UTR). Puf proteins are conserved throughout eukaryotes and have diverse roles including stem cell maintenance, neuronal development, stress response and organelle biogenesis. In Saccharomyces cerevisiae, Puf proteins are conditionally regulated in response to the cells metabolic. Specifically, in fermentative growth Puf3p is active to stimulate decay of target mRNAs; however, during respiration, Puf3p is inactive, resulting in mRNA stabilization. Presented herein, I show that in addition to Puf3p, the activity of Puf1p, Puf4p and Puf5p in yeast is conditionally regulated. I establish YHB1 mRNA as a bona fide target of Puf1p, Puf4p and Puf5p regulation through one unique binding site located in its 3’ UTR. These Puf proteins regulate YHB1 mRNA in response to nitric oxide stress. This data supports a model for Puf regulation of target mRNAs where transcripts are rapidly degraded in the absence of stress; however, in stress conditions transcripts are stabilized allowing increased protein production. I also show that Puf activity is regulated by two different mechanisms. First, the RNA binding activity of Puf5p is reduced during inactivating conditions; however, Puf1p, Puf3p and Puf4p bind target mRNAs regardless of stress. Puf proteins interact with decay machinery to facilitate mRNA degradation. The Puf3p interaction with Pop2p is disrupted in inactivating conditions and Pop2p is required to bridge interactions with other decay factors, thus preventing Puf3p stimulated decay. In a third project, I evaluated Puf proteins in humans, also called Pum proteins. I sought to identify natural targets of Pum regulation in humans. Utilizing bioinformatic and experimental approaches, I identified SNCA, LRRK2 and SAT1 as bona fide targets of Pum regulation. These mRNAs are all involved in Parkinson’s disease (PD). The implication of this discovery may provide a novel therapeutic approach to PD in the future.

Identification of New MRNA Targets of Puf3 Protein-mediated Decay and Analysis of Their Condition-specific Decay Regulation in Yeast

Download Identification of New MRNA Targets of Puf3 Protein-mediated Decay and Analysis of Their Condition-specific Decay Regulation in Yeast PDF Online Free

Author : Melanie A. Miller
Publisher :
ISBN 13 :
Total Pages : 101 pages
Book Rating : 4.:/5 (535 download)

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Book Synopsis Identification of New MRNA Targets of Puf3 Protein-mediated Decay and Analysis of Their Condition-specific Decay Regulation in Yeast by : Melanie A. Miller

Download or read book Identification of New MRNA Targets of Puf3 Protein-mediated Decay and Analysis of Their Condition-specific Decay Regulation in Yeast written by Melanie A. Miller and published by . This book was released on 2007 with total page 101 pages. Available in PDF, EPUB and Kindle. Book excerpt: The eukaryotic Puf proteins function to regulate gene expression by altering mRNA stability. Specifically, Puf proteins bind the 3' untranslated region (UTR) of mRNA targets to stimulate their turnover. In the yeast S. cerevisiae, six Puf proteins have been identified, in which Puf1p-Puf5p share a well conserved RNA-binding domain. The yeast Pufs regulate mRNA stability on a transcript-specific basis, though only a few mRNA targets have been experimentally verified. Yeast Puf3p was originally found to mediate rapid turnover of COX17 mRNA, which encodes a mitochondrial copper shuttle. More recently, microarray and computational analyses revealed that Puf3p physically associates with>100 nuclear-transcribed mRNAs that encode mitochondrial proteins. Most of these mRNAs contain one or more conserved putative Puf3p binding site(s) within their 3'UTRs, and it is predicted that their steady-state expression levels are altered by different growth conditions. In addition, Puf3p has been shown to localize to the cytoplasmic face of the mitochondrial membrane and affect its motility, suggesting that Puf3p may play an important role in regulating mitochondrial function. In this work, I have experimentally validated several new mRNAs that are targeted for turnover by Puf3p. I have also determined that these targets are regulated by Puf3p in a condition-specific manner. Specifically, I have analyzed the decay of fifteen putative Puf3p mRNA targets in the absence and presence of Puf3p, identifying transcripts that represent true targets of Puf3p-mediated mRNA decay. These transcripts are rapidly degraded in the presence of Puf3p and are stabilized in a puf3[delta] strain. Transcriptional pulse-chase experiments with one of the identified targets, CYT2, revealed that Puf3p destabilizes this target by stimulating deadenylation of the transcript and subsequent steps of decay, and this regulation only requires the CYT2 3'UTR. I have also monitored the stabilities of COX17, CYT2, and TUF1 mRNAs as reporters of Puf3p activity, and found that these RNAs are stabilized in ethanol, galactose, and raffinose conditions, suggesting that Puf3p activity is severely inhibited. Interestingly, Puf3p is rapidly activated or inactivated upon changing carbon sources to or from dextrose, respectively, as measured by CYT2 and TUF1 decay phenotypes. PUF3 mRNA and protein levels are not decreased in conditions that inhibit Puf3p activity, which suggests that Puf3p activity may be regulated post-translationally. Together, my work provides a greater understanding of the role of Puf3p in mRNA decay regulation, and provides insight into the mechanism of Puf3p activity. The significant structural and functional homology between Pufs suggests that the knowledge gained from my work on Puf3p in yeast will make contributions to understanding Puf protein regulation in higher eukaryotes as well.

The Role of Puf3 Protein Interactions in the Regulation of MRNA Decay in Yeast Saccharomyces Cerevisiae

Author : Shervin Sean Houshmandi
Publisher :
ISBN 13 :
Total Pages : 159 pages
Book Rating : 4.:/5 (525 download)

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Book Synopsis The Role of Puf3 Protein Interactions in the Regulation of MRNA Decay in Yeast Saccharomyces Cerevisiae by : Shervin Sean Houshmandi

Download or read book The Role of Puf3 Protein Interactions in the Regulation of MRNA Decay in Yeast Saccharomyces Cerevisiae written by Shervin Sean Houshmandi and published by . This book was released on 2005 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: The regulation of messenger RNA (mRNA) metabolism is an important step in proper gene expression. In many eukaryotic organisms this regulation can be mediated by a group of highly conserved RNA-binding proteins known as the Puf family of proteins. The yeast Saccharomyces cerevisiae has several proteins that belong to this family. One of the yeast Puf proteins, Puf3p, binds and regulates the COX17 mRNA, which codes for a protein involved in mitochondrial copper transport. Specifically, Puf3p stimulates the decay of COX17 mRNA. However, the precise mechanism of Puf3p binding and decay regulation is yet unknown. The goal of this research is to determine the role of the Puf3p interactions required for regulation of mRNA decay in yeast, and to understand how Puf3p activity is regulated. The studies to examine Puf3p interactions have focused on the Puf3 protein sequences required for specific binding and regulation of COX17 mRNA decay. The data show that a specific region of the Puf3 protein, known as the Puf3 Repeat Domain, is sufficient to both bind COX17 mRNA and also regulate its rate of decay. Furthermore, key amino acids on the RNA-binding surface of the repeat domain that promote target specificity have been identified, as well as a specific loop structure on the protein-binding surface of the repeat domain that is required for RNA decay regulation. In addition, these studies show that the repeat domain of Puf3p directly interacts with other known mRNA decay factors, more specifically, decay factors that are involved in the deadenylation and decapping steps of mRNA decay. Additional collaborative studies have focused on the condition-specific regulation of mRNA stability in yeast. In these studies, the activity of Puf3p was found to be dependent on the available carbon source, as well as inhibited by rapamycin treatment, which in turn places the Puf3p downstream of the Target of Rapamycin (TOR) signaling pathway. Together the results from the research in this body of work will help further our understanding of transcript-specific decay mechanisms in yeast and other eukaryotes.

Puf1p-mediated MRNA Decay and Combinatorial Control of MRNA Stability by the Yeast Puf Proteins

Author : Randi J. Ulbricht
Publisher :
ISBN 13 :
Total Pages : 137 pages
Book Rating : 4.:/5 (566 download)

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Book Synopsis Puf1p-mediated MRNA Decay and Combinatorial Control of MRNA Stability by the Yeast Puf Proteins by : Randi J. Ulbricht

Download or read book Puf1p-mediated MRNA Decay and Combinatorial Control of MRNA Stability by the Yeast Puf Proteins written by Randi J. Ulbricht and published by . This book was released on 2008 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: The stability of a messenger RNA (mRNA) is a highly regulated and important aspect of gene expression. Proteins that regulate mRNA stability often bind to 3' untranslated region (UTR) sequence elements. The eukaryotic Puf proteins are one class of 3'UTR binding proteins that regulate the stability and expression of their target transcripts. Several global genome analyses have identified hundreds of potential mRNA targets of the Saccharomyces cerevisiae Puf proteins, however only three mRNA targets for these proteins have been characterized thus far. After direct testing of nearly forty candidate mRNAs, I have established three of these as true mRNA targets of Puf-mediated decay in yeast, YHB1, HXK1 and TIF1. In a novel finding, multiple Puf proteins, including Puf1p, regulate HXK1 and TIF1 mRNAs in combination. TIF1 mRNA decay can be stimulated individually by Puf1p and Puf5p, but the combination of both proteins is required for full regulation. This Puf-mediated decay requires the presence of two UGUA binding sites within the TIF1 3'UTR, with one site regulated by Puf5p and the other by both Puf1p and Puf5p. The stability of the endogenously transcribed HXK1 mRNA, cellular levels of Hxk1 protein activity, and HXK1 3'UTR-directed decay are affected by Puf1p and Puf5p as well as Puf4p. YHB1 mRNA decay is mediated by Puf5p and also requires a UGUA sequence element. This work has discovered the first targets of Puf1-mediated decay. Since much of our knowledge of the mechanism suggests that Puf protein target recognition and mechanism of action varies with each of these proteins, I investigate the mechanism of Puf1p-mediated decay using a variety a techniques. The results of this research aid in our understanding of the similar, yet distinct, decay regulation of Puf proteins in yeast and higher organisms.

RNA-protein Interactions

Author : Kiyoshi Nagai
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 302 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis RNA-protein Interactions by : Kiyoshi Nagai

Download or read book RNA-protein Interactions written by Kiyoshi Nagai and published by Oxford University Press, USA. This book was released on 1994 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.

RNA Tagging

Author : Manfred Heinlein
Publisher : Humana
ISBN 13 : 9781071607145
Total Pages : 490 pages
Book Rating : 4.6/5 (71 download)

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Book Synopsis RNA Tagging by : Manfred Heinlein

Download or read book RNA Tagging written by Manfred Heinlein and published by Humana. This book was released on 2021-08-08 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.

Prokaryotic Metabolism and Physiology

Author : Byung Hong Kim
Publisher : Cambridge University Press
ISBN 13 : 1107171733
Total Pages : 509 pages
Book Rating : 4.1/5 (71 download)

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Book Synopsis Prokaryotic Metabolism and Physiology by : Byung Hong Kim

Download or read book Prokaryotic Metabolism and Physiology written by Byung Hong Kim and published by Cambridge University Press. This book was released on 2019-05-16 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensive and up-to-date review of key metabolic processes in bacteria and archaea and how metabolism is regulated under various conditions.

MicroRNAs in Cancer

Author : Cesar Lopez-Camarillo
Publisher : CRC Press
ISBN 13 : 1466576774
Total Pages : 426 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis MicroRNAs in Cancer by : Cesar Lopez-Camarillo

Download or read book MicroRNAs in Cancer written by Cesar Lopez-Camarillo and published by CRC Press. This book was released on 2013-02-22 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: MicroRNA (miRNA) biology is a cutting-edge topic in basic as well as biomedical research. This is a specialized book focusing on the current understanding of the role of miRNAs in the development, progression, invasion, and metastasis of diverse types of cancer. It also reviews their potential for applications in cancer diagnosis, prognosis, and th

Genetics, Neurology, Behavior, and Diet in Parkinson's Disease

Author : Colin R Martin
Publisher : Academic Press
ISBN 13 : 0128159510
Total Pages : 764 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Genetics, Neurology, Behavior, and Diet in Parkinson's Disease by : Colin R Martin

Download or read book Genetics, Neurology, Behavior, and Diet in Parkinson's Disease written by Colin R Martin and published by Academic Press. This book was released on 2020-08-13 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetics, Neurology, Behavior, and Diet in Parkinson's Disease: The Neuroscience of Parkinson’s Disease, Volume 2 provides a single source of material covering different scientific domains of neuropathology underlying this condition. The book covers a wide range of subjects and unravels the complex relationships between genetics, molecular biology, pharmaceutical chemistry, neurobiology, imaging, assessments, and treatment regimens. It fills a much-needed gap as a "one-stop" synopsis of everything to do with the neurology and neuroscience related to Parkinson’s disease—from chemicals and cells to individuals. It is an invaluable resource for neuroscientists, neurologists, and anyone in the field. Offers the most comprehensive coverage of a broad range of topics related to Parkinson's disease Serves as a foundational collection for neuroscientists and neurologists on the biology of disease and brain dysfunction Contains in each chapter an abstract, key facts, mini dictionary of terms, and summary points to aid in understanding Features preclinical and clinical studies to help researchers map out key areas for research and further clinical recommendations Serves as a "one-stop" source for everything you need to know about Parkinson’s disease

Plant RNA Biology

Author : Dóra Szakonyi
Publisher : Frontiers Media SA
ISBN 13 : 2889630250
Total Pages : 167 pages
Book Rating : 4.8/5 (896 download)

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Book Synopsis Plant RNA Biology by : Dóra Szakonyi

Download or read book Plant RNA Biology written by Dóra Szakonyi and published by Frontiers Media SA. This book was released on 2019-11-18 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discoveries from the past decades revealed that RNA molecules are much more than inert intermediates between the coding DNA sequences and their functional products, proteins. Today, RNAs are recognized as active regulatory molecules influencing gene expression, chromatin organization and genome stability, thus impacting all aspects of plant life including development, growth, reproduction and stress tolerance. Innovations in methodologies, the expanding application of next-generation sequencing technologies, and the creation of public datasets and databases have exposed a new universe of RNA-based mechanisms and led to the discovery of new families of non-coding RNAs, uncovered the large extent of alternative splicing events, and highlighted the potential roles of RNA modifications and RNA secondary structures. Furthermore, considerable advances have been made in identifying RNA-binding and processing factors involved in the synthesis and maturation of different forms of RNA molecules as well as in RNA processing, biochemical modifications or degradation. This Research Topic showcases the broad biological significance of RNAs in plant systems and contains eight original research articles, one review and four mini-reviews, covering various RNA-based mechanisms in higher plants. Emerging new technologies and novel multidisciplinary approaches are empowering the scientific community and will expectedly bring novel insights into our understanding of the mechanisms through which RNA is regulated and regulates biological processes in plant cells.

How Tobacco Smoke Causes Disease

Author : United States. Public Health Service. Office of the Surgeon General
Publisher :
ISBN 13 :
Total Pages : 728 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis How Tobacco Smoke Causes Disease by : United States. Public Health Service. Office of the Surgeon General

Download or read book How Tobacco Smoke Causes Disease written by United States. Public Health Service. Office of the Surgeon General and published by . This book was released on 2010 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report considers the biological and behavioral mechanisms that may underlie the pathogenicity of tobacco smoke. Many Surgeon General's reports have considered research findings on mechanisms in assessing the biological plausibility of associations observed in epidemiologic studies. Mechanisms of disease are important because they may provide plausibility, which is one of the guideline criteria for assessing evidence on causation. This report specifically reviews the evidence on the potential mechanisms by which smoking causes diseases and considers whether a mechanism is likely to be operative in the production of human disease by tobacco smoke. This evidence is relevant to understanding how smoking causes disease, to identifying those who may be particularly susceptible, and to assessing the potential risks of tobacco products.

Patterning and Cell Type Specification in the Developing CNS and PNS

Author :
Publisher : Academic Press
ISBN 13 : 0123973481
Total Pages : 993 pages
Book Rating : 4.1/5 (239 download)

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Book Synopsis Patterning and Cell Type Specification in the Developing CNS and PNS by :

Download or read book Patterning and Cell Type Specification in the Developing CNS and PNS written by and published by Academic Press. This book was released on 2013-05-06 with total page 993 pages. Available in PDF, EPUB and Kindle. Book excerpt: The genetic, molecular, and cellular mechanisms of neural development are essential for understanding evolution and disorders of neural systems. Recent advances in genetic, molecular, and cell biological methods have generated a massive increase in new information, but there is a paucity of comprehensive and up-to-date syntheses, references, and historical perspectives on this important subject. The Comprehensive Developmental Neuroscience series is designed to fill this gap, offering the most thorough coverage of this field on the market today and addressing all aspects of how the nervous system and its components develop. Particular attention is paid to the effects of abnormal development and on new psychiatric/neurological treatments being developed based on our increased understanding of developmental mechanisms. Each volume in the series consists of review style articles that average 15-20pp and feature numerous illustrations and full references. Volume 1 offers 48 high level articles devoted mainly to patterning and cell type specification in the developing central and peripheral nervous systems. Series offers 144 articles for 2904 full color pages addressing ways in which the nervous system and its components develop Features leading experts in various subfields as Section Editors and article Authors All articles peer reviewed by Section Editors to ensure accuracy, thoroughness, and scholarship Volume 1 sections include coverage of mechanisms which: control regional specification, regulate proliferation of neuronal progenitors and control differentiation and survival of specific neuronal subtypes, and controlling development of non-neural cells

The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use

Author : Elizabeth H. Harris
Publisher : Academic Press
ISBN 13 : 0080919553
Total Pages : 483 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use by : Elizabeth H. Harris

Download or read book The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use written by Elizabeth H. Harris and published by Academic Press. This book was released on 2009-03-07 with total page 483 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. Expanded revision of gold standard reference Includes latest advances in research, including completion of the genome Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields Available as part of a 3-Volume Set or sold individually

Regulation of Photosynthesis

Author : Eva-Mari Aro
Publisher : Springer Science & Business Media
ISBN 13 : 0306481480
Total Pages : 624 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Regulation of Photosynthesis by : Eva-Mari Aro

Download or read book Regulation of Photosynthesis written by Eva-Mari Aro and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the expression of photosynthesis related genes including regulation both at transcriptional and translational levels. It reviews biogenesis, turnover, and senescence of thylakoid pigment protein complexes and highlights some crucial regulatory steps in carbon metabolism.

Post-transcriptional Mechanisms in Endocrine Regulation

Author : K.M.J. Menon, PhD
Publisher : Springer
ISBN 13 : 3319251244
Total Pages : 347 pages
Book Rating : 4.3/5 (192 download)

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Book Synopsis Post-transcriptional Mechanisms in Endocrine Regulation by : K.M.J. Menon, PhD

Download or read book Post-transcriptional Mechanisms in Endocrine Regulation written by K.M.J. Menon, PhD and published by Springer. This book was released on 2015-12-30 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines how post-transcriptional mechanisms control endocrine function. This includes newly identified regulatory mechanisms involved in hormone biosynthesis, control of hormone receptors and the outputs of hormone mediated signal transduction. Chapters address endocrine hormones including protein peptide/peptide, steroid, and non-steroidal hormones. The impacts of these mechanisms on disease and health are covered, providing a novel update to the scientific literature. Post-transcriptional regulatory mechanisms play an essential role in controlling dynamic gene expression. The outcome of this regulation includes control of the amount, timing, and location of protein expression. Regulation is mediated by cis-acting RNA sequences and structures and transacting RNA binding proteins and non-coding RNAs, including microRNAs. Recent advances in characterization of these regulatory factors have revealed enormous regulatory potential.

RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways

Author : Lynne E. Maquat
Publisher : Academic Press
ISBN 13 : 9780123745842
Total Pages : 464 pages
Book Rating : 4.7/5 (458 download)

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Book Synopsis RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways by : Lynne E. Maquat

Download or read book RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways written by Lynne E. Maquat and published by Academic Press. This book was released on 2008-12-29 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. * Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway * Expert researchers introduce the most advanced technologies and techniques * Offers step-by-step lab instructions, including necessary equipment and reagents

Vertebrate Development

Author : Francisco Pelegri
Publisher : Springer
ISBN 13 : 3319460951
Total Pages : 554 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Vertebrate Development by : Francisco Pelegri

Download or read book Vertebrate Development written by Francisco Pelegri and published by Springer. This book was released on 2016-12-13 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of topics describing the earliest steps of fertilization, from egg activation and fertilization to the activation of the zygotic genome, in various studied vertebrate model systems. The contribution of maternal and paternal factors and their role in the early embryo as parental DNA becomes modified and embryonic genes become activated is fundamental to the initiation of embryogenesis in all animal systems. It can be argued that this is a unique developmental period, when information from the parents is compressed to direct the development of the body plan of the entire organism, a process of astounding simplicity, elegance and beauty. In addition to their fundamental scientific interest, many frontiers of biomedicine, such as reproductive biology, stem cells and reprogramming, and the understanding of intergenerational diseases, depend on advances in our knowledge of these early processes. Vertebrate Development: Maternal to Zygotic Control brings together chapters from experts in various disciplines describing the latest advances related to this important developmental transition. Each chapter is a synthesis of knowledge relevant to all vertebrates, with details on specific systems as well as comparisons between the various studied vertebrate models. The editorial expertise encompasses the fields of major vertebrate model systems (mammalian, amphibian and teleost) ensuring a balanced approach to various topics. This unique book—with its combination of in-depth and up-to-date basic research, inter-species comprehensiveness and emphasis on the very early stages of animal development—is essential for research scientists studying vertebrate development, as well as being a valuable resource for college educators teaching advanced courses in developmental biology.