Mass Spectrometry Developmental Approaches To Answer Biological Questions

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Mass Spectrometry: Developmental Approaches to Answer Biological Questions

Author : Gwenael Pottiez
Publisher : Springer
ISBN 13 : 3319130870
Total Pages : 71 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Mass Spectrometry: Developmental Approaches to Answer Biological Questions by : Gwenael Pottiez

Download or read book Mass Spectrometry: Developmental Approaches to Answer Biological Questions written by Gwenael Pottiez and published by Springer. This book was released on 2015-02-05 with total page 71 pages. Available in PDF, EPUB and Kindle. Book excerpt: The understanding of the events taking place in a cell, a biological fluid or in any biological system is the main goal of biology research. Many fields of research use different technology to assess those events. Mass spectrometry is one of those techniques and this undergoes constant evolution and adaptation to always enhance the accuracy of the information provided. Proteomics provides a large panel of data on protein identity and protein expression that were made possible by mass spectrometry. For several years now mass spectrometry has become central to performing proteomic research, however this powerful tool is under constant evolution to be more sensitive and more resolute. More importantly mass spectrometry became a field of research focusing on new applications. Indeed, the complexity in biological systems relies on the changes of expression of transcription of proteins but also on the post-translational modification of proteins, the structure of proteins and the interaction between proteins, amongst others. As of now, several investigations tried to improve the quantification of proteins by mass spectrometry, the determination of post-translational modifications, the protein-protein and protein-nucleic acids interaction or the proteins structures. This book is structured as follows: after a brief introduction of the usual and most popular applications for mass spectrometry in proteomics, the most recent research and developments in mass spectrometry-based methodologies will be explored.

Biomolecular and Bioanalytical Techniques

Author : Vasudevan Ramesh
Publisher : John Wiley & Sons
ISBN 13 : 1119483980
Total Pages : 576 pages
Book Rating : 4.1/5 (194 download)

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Book Synopsis Biomolecular and Bioanalytical Techniques by : Vasudevan Ramesh

Download or read book Biomolecular and Bioanalytical Techniques written by Vasudevan Ramesh and published by John Wiley & Sons. This book was released on 2019-03-08 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.

Protein and Peptide Mass Spectrometry in Drug Discovery

Author : Michael L. Gross
Publisher : John Wiley & Sons
ISBN 13 : 1118116542
Total Pages : 484 pages
Book Rating : 4.1/5 (181 download)

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Book Synopsis Protein and Peptide Mass Spectrometry in Drug Discovery by : Michael L. Gross

Download or read book Protein and Peptide Mass Spectrometry in Drug Discovery written by Michael L. Gross and published by John Wiley & Sons. This book was released on 2011-09-26 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discovery An instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery. Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.

Molecular Biophysics for the Life Sciences

Author : Norma Allewell
Publisher : Springer Science & Business Media
ISBN 13 : 1461485487
Total Pages : 401 pages
Book Rating : 4.4/5 (614 download)

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Book Synopsis Molecular Biophysics for the Life Sciences by : Norma Allewell

Download or read book Molecular Biophysics for the Life Sciences written by Norma Allewell and published by Springer Science & Business Media. This book was released on 2013-09-28 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview of the development and scope of molecular biophysics and in-depth discussions of the major experimental methods that enable biological macromolecules to be studied at atomic resolution. It also reviews the physical chemical concepts that are needed to interpret the experimental results and to understand how the structure, dynamics, and physical properties of biological macromolecules enable them to perform their biological functions. Reviews of research on three disparate biomolecular machines—DNA helicases, ATP synthases, and myosin--illustrate how the combination of theory and experiment leads to new insights and new questions.

Methods in Molecular Biophysics

Author : Nathan R. Zaccai
Publisher : Cambridge University Press
ISBN 13 : 1108508804
Total Pages : 710 pages
Book Rating : 4.1/5 (85 download)

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Book Synopsis Methods in Molecular Biophysics by : Nathan R. Zaccai

Download or read book Methods in Molecular Biophysics written by Nathan R. Zaccai and published by Cambridge University Press. This book was released on 2017-05-18 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current techniques for studying biological macromolecules and their interactions are based on the application of physical methods, ranging from classical thermodynamics to more recently developed techniques for the detection and manipulation of single molecules. Reflecting the advances made in biophysics research over the past decade, and now including a new section on medical imaging, this new edition describes the physical methods used in modern biology. All key techniques are covered, including mass spectrometry, hydrodynamics, microscopy and imaging, diffraction and spectroscopy, electron microscopy, molecular dynamics simulations and nuclear magnetic resonance. Each method is explained in detail using examples of real-world applications. Short asides are provided throughout to ensure that explanations are accessible to life scientists, physicists and those with medical backgrounds. The book remains an unparalleled and comprehensive resource for graduate students of biophysics and medical physics in science and medical schools, as well as for research scientists looking for an introduction to techniques from across this interdisciplinary field.

Medical Applications of Mass Spectrometry

Author : Karoly Vekey
Publisher : Elsevier
ISBN 13 : 0080554652
Total Pages : 606 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Medical Applications of Mass Spectrometry by : Karoly Vekey

Download or read book Medical Applications of Mass Spectrometry written by Karoly Vekey and published by Elsevier. This book was released on 2011-08-11 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry is fast becoming an indispensable field for medical professionals. The mass spectrometric analysis of metabolites and proteins promises to revolutionize medical research and clinical diagnostics. As this technology rapidly enters the medical field, practicing professionals and students need to prepare to take full advantage of its capabilities. Medical Applications of Mass Spectrometry addresses the key issues in the medical applications of mass spectrometry at the level appropriate for the intended readership. It will go a long way to help the utilization of mass spectrometry in medicine. The book comprises five parts. A general overview is followed by a description of the basic sampling and separation methods in analytical chemistry. In the second part a solid foundation in mass spectrometry and modern techniques of data analysis is presented. The third part explains how mass spectrometry is used in exploring various classes of biomolecules, including proteins and lipids. In the fourth section mass spectrometry is introduced as a diagnostic tool in clinical treatment, infectious pathogen research, neonatal diagnostics, cancer, brain and allergy research, as well as in various fields of medicine: cardiology, pulmonology, neurology, psychiatric diseases, hemato-oncology, urologic diseases, gastrointestinal diseases, gynecology and pediatrics. The fifth part covers emerging applications in biomarker discovery and in mass spectrometric imaging. * Provides a broad look at how the medical field is benefiting from advances in mass spectrometry. * Guides the reader from basic principles and methods to cutting edge applications. * There is NO comparable book on the market to fill this fast growing field.

The Design and Development of Novel Drugs and Vaccines

Author : Tarun Kumar Bhatt
Publisher : Academic Press
ISBN 13 : 0128214759
Total Pages : 310 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis The Design and Development of Novel Drugs and Vaccines by : Tarun Kumar Bhatt

Download or read book The Design and Development of Novel Drugs and Vaccines written by Tarun Kumar Bhatt and published by Academic Press. This book was released on 2021-01-21 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Design and Development of Novel Drugs and Vaccines: Principles and Protocols presents both in silico methods and experimental protocols for vaccine and drug design and development, critically reviewing the most current research and emphasizing approaches and technologies that accelerate and lower the cost of product development. Sections review the technologies and approaches used to identify, characterize and establish a protein as a new drug and vaccine target, cover several molecular methods for in vitro studies of the desired target, and present various physiological parameters for in vivo studies. The book includes preclinical trials and research, along with information on FDA approval. Covers both in silico methods and experimental protocols for vaccine and drug development in a single, accessible volume Offers a holistic accounting of how developments in bioinformatics and large experimental datasets can be used in the development of vaccines and drugs Shows researchers the entire gamut of current therapies, ranging from computational inputs to animal studies Reviews the most current, cutting-edge research available on vaccine and drug design and development

Plant metabolomics in full swing

Author :
Publisher : Academic Press
ISBN 13 : 0128232455
Total Pages : 374 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Plant metabolomics in full swing by :

Download or read book Plant metabolomics in full swing written by and published by Academic Press. This book was released on 2021-01-24 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Metabolics, Volume 98, the latest release in the Advances in Botanical Research series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of intriguing topics, including Developmental metabolomics to decipher and improve fleshy fruit quality, Specialized metabolites in seeds, Untangling plant immune responses through metabolomics, Plant metabolomics to the benefit of crop protection and growth stimulation, Metabolomics in plant-microbe interactions in the roots, A practical guide to implementing metabolomics in plant ecology and biodiversity research, Plant metabolomics and breeding, Plant genome-scale metabolic networks, Metabolite imaging by mass spectrometry: A new discovery tool, MS- and NMR-metabolomic tools for the discrimination of wines: Applications for authenticity Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Advances in Botanical Research series Updated release includes the latest information on the Plant Metabolics

Encyclopedia of Data Warehousing and Mining, Second Edition

Author : Wang, John
Publisher : IGI Global
ISBN 13 : 1605660116
Total Pages : 2542 pages
Book Rating : 4.6/5 (56 download)

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Book Synopsis Encyclopedia of Data Warehousing and Mining, Second Edition by : Wang, John

Download or read book Encyclopedia of Data Warehousing and Mining, Second Edition written by Wang, John and published by IGI Global. This book was released on 2008-08-31 with total page 2542 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are more than one billion documents on the Web, with the count continually rising at a pace of over one million new documents per day. As information increases, the motivation and interest in data warehousing and mining research and practice remains high in organizational interest. The Encyclopedia of Data Warehousing and Mining, Second Edition, offers thorough exposure to the issues of importance in the rapidly changing field of data warehousing and mining. This essential reference source informs decision makers, problem solvers, and data mining specialists in business, academia, government, and other settings with over 300 entries on theories, methodologies, functionalities, and applications.

New and Future Developments in Microbial Biotechnology and Bioengineering

Author : Vijai Kumar Gupta
Publisher : Elsevier
ISBN 13 : 0444635130
Total Pages : 315 pages
Book Rating : 4.4/5 (446 download)

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Book Synopsis New and Future Developments in Microbial Biotechnology and Bioengineering by : Vijai Kumar Gupta

Download or read book New and Future Developments in Microbial Biotechnology and Bioengineering written by Vijai Kumar Gupta and published by Elsevier. This book was released on 2016-10-27 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: New and Future Developments in Microbial Biotechnology and Bioengineering: Aspergillus System Properties and Applications provides information on emerging issues related to recent advancements in aspergillus research and its applications in bioprocess technology, chemical engineering, genome biology, molecular taxonomy, secondary and metabolite production, industrial process and biofuels/bioenergy research, and alternative fuel development. The book covers the various novel enzymes secreted by these fungi and their specific use in the food, textile, pulp and paper, biocellulosic ethanol production, and other industries. The book describes research and experimentation on aspergillus activity and directly connects them to their use in bioprocess technology, chemical engineering, bioremediation process, secondary metabolite production, pharmaceutical processes, protein production, industrial process, biofuels/bioenergy research, and alternative fuel development. Readers will find this book to be an indispensable resource for biotechnologists, biochemical engineers, biochemists, microbiologists, bioinformatics researchers, and other biologists who are interested in learning about the potential applications of these fungi. Compiles available, up-to-date information on recent developments made in the study of aspergillus system properties Contains global content from pioneering international authors Presents current research efforts and links them to various applications, including uses in foods, textiles, pulp and paper, and in biocellulosic ethanol production Provides an indispensable resource for biologists who are interested in learning about the potential applications of the fungi aspergillus

Proteomics as a Multifaceted Tool in Medicine and Environmental Assessment

Author : Jacob Kuruvilla
Publisher : Linköping University Electronic Press
ISBN 13 : 9176854582
Total Pages : 67 pages
Book Rating : 4.1/5 (768 download)

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Book Synopsis Proteomics as a Multifaceted Tool in Medicine and Environmental Assessment by : Jacob Kuruvilla

Download or read book Proteomics as a Multifaceted Tool in Medicine and Environmental Assessment written by Jacob Kuruvilla and published by Linköping University Electronic Press. This book was released on 2017-01-01 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics is evolving as a multi-faceted tool for addressing various biochemical and biomedical queries in the field of scientific research. This involves various stages, ranging from sample preparation to data analysis and biological interpretation. Sample preparation involves isolating proteins from the sample source, purifying and digesting them to initiate shotgun proteomics. Shotgun proteomics identifies proteins by bottom-up proteomic approaches where proteins are identified from the fragmentation spectra of their own peptides. Paper I: deals with the simplification of functional characterization for nanoparticles intended for use in biomedicine. Proteomics was constructive in differentiating and semi-quantifying the surface of protein corona. This could be beneficial in predicting the interactions between nanoparticles and a biological entity like the cell or a receptor protein and provide initial valuable information related to targeting, uptake and safety. Paper II: deals with understanding effects of TiO2 nanoparticles on endothelial cells. A combinatorial approach, involving transcriptomics and proteomics was used to identify aberrations in the permeability and integrity of endothelial cells and tissues. Our study also investigated the correlation of size and how they motivated a differential cellular response. In case of intravenous entry for nanoparticles in targeted drug delivery systems, endothelial cells are the first barrier encountered by these drug carriers. This evaluation involving endothelial cell response could be very instrumental during the designing of NP based drug delivery systems. Paper III: Pharmaceuticals and its metabolites could be very hazardous, especially if its disposal is not managed properly. Since water bodies are the ultimate sink, these chemicals could end up there, culminating in toxicity and other ‘mixture effects’ in combination with other factors. To evaluate the effects of the pharmaceutical, propranolol and climatic factors like low salinity conditions, a microcosm exposure was designed and shotgun proteomics helped understand its impact on mussel gills. In this study too, a combination of transcriptomics and proteomics unveiled molecular mechanisms altered in response to stressors, both individually and in combination. Paper IV: An interplay of various factors like EBF1 and PAX5 determines B-cell lineage and commitment. This might have been materialized by direct and transient proteinprotein interactions. A unique method called BioID helped screen relevant interactions in living cells by the application of a promiscuous biotin ligase enzyme capable of tagging proteins through biotinylation based on a proximity radius. Biotinylation of endogenous proteins enabled their selective isolation by exploiting the high affinity of biotin and streptavidin on streptavidin coated agarose beads, leading to their identification by mass spectrometry. The biotinylated proteins were potential candidate interactors of EBF1 and PAX5, which were later confirmed by sequencing techniques like ChIP-Seq, ATAC seq, and visualization techniques like proximity ligation assay (PLA).

Genomic and Precision Medicine

Author : Geoffrey S. Ginsburg
Publisher : Academic Press
ISBN 13 : 0128006560
Total Pages : 398 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Genomic and Precision Medicine by : Geoffrey S. Ginsburg

Download or read book Genomic and Precision Medicine written by Geoffrey S. Ginsburg and published by Academic Press. This book was released on 2016-11-22 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genomic and Precision Medicine: Translation and Implementation highlights the various points along the continuum from health to disease where genomic information is impacting clinical decision-making and leading to more personalization of health care. The book pinpoints the challenges, barriers, and solutions that have been, or are being, brought forward to enable translation of genome based technologies into health care. A variety of infrastructure (data systems and EMRs), policy (regulatory, reimbursement, privacy), and research (comparative effectiveness research, learning health system approaches) strategies are also discussed. Readers will find this volume to be an invaluable resource for the translational genomics and implementation science that is required to fully realize personalized health care. Provides a comprehensive volume on the translation and implementation of biology into health care provision Presents succinct commentary and key learning points that will assist readers with their local needs for translation and implementation Includes an up-to-date overview on major ‘translational events’ in genomic and personalized medicine, along with lessons learned

Metallomics

Author : Yasumitsu Ogra
Publisher : Springer
ISBN 13 : 4431564632
Total Pages : 364 pages
Book Rating : 4.4/5 (315 download)

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Book Synopsis Metallomics by : Yasumitsu Ogra

Download or read book Metallomics written by Yasumitsu Ogra and published by Springer. This book was released on 2017-03-01 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on recent topics in metallomics, a study of the metallome, or metal-containing biomolecules. Metals can induce various physiological and toxicological effects in a very small amounts, in other words, the concentrations of biometals are very low in organisms. Thus, analytical techniques for a trace amount of metal are crucial to understand the biological and toxicological functions of metals.This volume begins with an overview of metallomics including the history and development of the field. Subsequent parts provide basic and advanced techniques for metallomics. Speciation and imaging of metals are basic approaches to reveal the function of the metallome. The applications of speciation using an HPLC hyphenated with inductively coupled plasma mass spectrometry (LC-ICP-MS) and flow cytometry ICP-MS are described. As advanced approaches, the applications using a micro-flow injection-ICP-MS, an ICP-triple quadrupole mass spectrometer, an ICP-sector field mass spectrometer, and an ICP-multi-collector mass spectrometer are mentioned. For the imaging of metals, basic principles and applications of several techniques such as scanning X-ray fluorescence microscopy and ICP-MS equipped with laser ablation (LA-ICP-MS) are presented. Speciation analyses using electrospray ionization mass spectrometry (ESI-MS), X-ray Absorption Spectroscopy (XAS), and nuclear magnetic resonance spectroscopy (NMR) are also introduced. The last part highlights the medical and pharmaceutical applications of metallomics. Molecular biological approaches to reveal the effects of toxic metals, metal functions in brain and neurodegenerative diseases, and metallodrugs are explained. The topic of metal transporters is also presented.

Information Resources in Toxicology, Volume 1: Background, Resources, and Tools

Author : Steve Gilbert
Publisher : Academic Press
ISBN 13 : 0128137258
Total Pages : 1055 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Information Resources in Toxicology, Volume 1: Background, Resources, and Tools by : Steve Gilbert

Download or read book Information Resources in Toxicology, Volume 1: Background, Resources, and Tools written by Steve Gilbert and published by Academic Press. This book was released on 2020-05-16 with total page 1055 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new fifth edition of Information Resources in Toxicology offers a consolidated entry portal for the study, research, and practice of toxicology. Both volumes represents a unique, wide-ranging, curated, international, annotated bibliography, and directory of major resources in toxicology and allied fields such as environmental and occupational health, chemical safety, and risk assessment. The editors and authors are among the leaders of the profession sharing their cumulative wisdom in toxicology’s subdisciplines. This edition keeps pace with the digital world in directing and linking readers to relevant websites and other online tools. Due to the increasing size of the hardcopy publication, the current edition has been divided into two volumes to make it easier to handle and consult. Volume 1: Background, Resources, and Tools, arranged in 5 parts, begins with chapters on the science of toxicology, its history, and informatics framework in Part 1. Part 2 continues with chapters organized by more specific subject such as cancer, clinical toxicology, genetic toxicology, etc. The categorization of chapters by resource format, for example, journals and newsletters, technical reports, organizations constitutes Part 3. Part 4 further considers toxicology’s presence via the Internet, databases, and software tools. Among the miscellaneous topics in the concluding Part 5 are laws and regulations, professional education, grants and funding, and patents. Volume 2: The Global Arena offers contributed chapters focusing on the toxicology contributions of over 40 countries, followed by a glossary of toxicological terms and an appendix of popular quotations related to the field. The book, offered in both print and electronic formats, is carefully structured, indexed, and cross-referenced to enable users to easily find answers to their questions or serendipitously locate useful knowledge they were not originally aware they needed. Among the many timely topics receiving increased emphasis are disaster preparedness, nanotechnology, -omics, risk assessment, societal implications such as ethics and the precautionary principle, climate change, and children’s environmental health. Introductory chapters provide a backdrop to the science of toxicology, its history, the origin and status of toxicoinformatics, and starting points for identifying resources Offers an extensive array of chapters organized by subject, each highlighting resources such as journals, databases,organizations, and review articles Includes chapters with an emphasis on format such as government reports, general interest publications, blogs, and audiovisuals Explores recent internet trends, web-based databases, and software tools in a section on the online environment Concludes with a miscellany of special topics such as laws and regulations, chemical hazard communication resources, careers and professional education, K-12 resources, funding, poison control centers, and patents Paired with Volume Two, which focuses on global resources, this set offers the most comprehensive compendium of print, digital, and organizational resources in the toxicological sciences with over 120 chapters contributions by experts and leaders in the field

Bioanalytics

Author : Friedrich Lottspeich
Publisher : John Wiley & Sons
ISBN 13 : 3527339191
Total Pages : 1151 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Bioanalytics by : Friedrich Lottspeich

Download or read book Bioanalytics written by Friedrich Lottspeich and published by John Wiley & Sons. This book was released on 2018-05-29 with total page 1151 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Systems Biology Approaches to Understanding the Cause and Treatment of Heart, Lung, Blood, and Sleep Disorders

Author : Raimond L Winslow
Publisher : Frontiers E-books
ISBN 13 : 2889192490
Total Pages : 181 pages
Book Rating : 4.8/5 (891 download)

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Book Synopsis Systems Biology Approaches to Understanding the Cause and Treatment of Heart, Lung, Blood, and Sleep Disorders by : Raimond L Winslow

Download or read book Systems Biology Approaches to Understanding the Cause and Treatment of Heart, Lung, Blood, and Sleep Disorders written by Raimond L Winslow and published by Frontiers E-books. This book was released on 2014-11-21 with total page 181 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of powerful new high- throughput technologies for probing the transcriptome, proteome and metabolome is driving the rapid acquisition of information on the function of molecular systems. The importance of these achievements cannot be understated – they have transformed the nature of both biology and medicine. Despite this dramatic progress, one of the greatest challenges that continues to confront modern biology is to understand how behavior at the level of genome, proteome and metabolome determines physiological function at the level of cell, tissue and organ in both health and disease. Because of the inherent complexity of biological systems, the development, analysis, and validation of integrative computational models based directly on experimental data is necessary to achieve this understanding. This approach, known as systems biology, integrates computational and experimental approaches through iterative development of mathematical models and experimental validation and testing. The combination of these approaches allows for a mechanistic understanding of the function of complex biological systems in health and their dysfunction in disease. The National Heart, Lung, and Blood Institute (NHLBI) has recognized the importance of the systems biology approach for understanding normal physiology and perturbations associated with heart, lung, blood, and sleep diseases and disorders. In 2006, NHLBI announced the Exploratory Program in Systems Biology, followed in 2010 by the NHLBI Systems Biology Collaborations. The goal of these programs is to support collaborative teams of investigators in using experimental and computational strategies to integrate the component parts of biological networks and pathways into computational models that are based firmly on and validated using experimental data. These validated models are then applied to gain insights into the mechanisms of altered system function in disease, to generate novel hypotheses regarding these mechanisms that can be tested experimentally, and to then use the results of experiments to refine the models. The purpose of this Research Topic is to present the range of innovative, new approaches being developed by investigators working in areas of systems biology that couple experimental and modeling studies to understand the cause and possible treatment of heart, lung, blood and sleep diseases and disorders. This Research Topic will be of great interest to the cardiovascular research community as well as to the general community of systems biologists.

Heart Development and Regeneration

Author : Nadia Rosenthal
Publisher : Academic Press
ISBN 13 : 0123813336
Total Pages : 1126 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Heart Development and Regeneration by : Nadia Rosenthal

Download or read book Heart Development and Regeneration written by Nadia Rosenthal and published by Academic Press. This book was released on 2010-05-28 with total page 1126 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of the cardiovascular system is a rapidly advancing area in biomedical research, now coupled with the burgeoning field of cardiac regenerative medicine. A lucid understanding of these fields is paramount to reducing human cardiovascular diseases of both fetal and adult origin. Significant progress can now be made through a comprehensive investigation of embryonic development and its genetic control circuitry. Heart Development and Regeneration, written by experts in the field, provides essential information on topics ranging from the evolution and lineage origins of the developing cardiovascular system to cardiac regenerative medicine. A reference for clinicians, medical researchers, students, and teachers, this publication offers broad coverage of the most recent advances. Volume One discusses heart evolution, contributing cell lineages; model systems; cardiac growth; morphology and asymmetry; heart patterning; epicardial, vascular, and lymphatic development; and congenital heart diseases. Volume Two includes chapters on transcription factors and transcriptional control circuits in cardiac development and disease; epigenetic modifiers including microRNAs, genome-wide mutagenesis, imaging, and proteomics approaches; and the theory and practice of stem cells and cardiac regeneration. Authored by world experts in heart development and disease New research on epigenetic modifiers in cardiac development Comprehensive coverage of stem cells and prospects for cardiac regeneration Up-to-date research on transcriptional and proteomic circuits in cardiac disease Full-color, detailed illustrations