Low-Temperature Microscopy and Analysis

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Publisher : Springer Science & Business Media
ISBN 13 : 1489923020
Total Pages : 553 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis Low-Temperature Microscopy and Analysis by : Patrick Echlin

Download or read book Low-Temperature Microscopy and Analysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.

Low Temperature Biological Microscopy

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Publisher :
ISBN 13 :
Total Pages : 320 pages
Book Rating : 4.:/5 (31 download)

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Book Synopsis Low Temperature Biological Microscopy by : Patrick Echlin

Download or read book Low Temperature Biological Microscopy written by Patrick Echlin and published by . This book was released on 1977 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Cryotechniques in Biological Electron Microscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 3642728154
Total Pages : 308 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Cryotechniques in Biological Electron Microscopy by : Rudolf A. Steinbrecht

Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Biomedical Applications of Microprobe Analysis

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Publisher : Academic Press
ISBN 13 : 0080524567
Total Pages : 573 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Biomedical Applications of Microprobe Analysis by : Peter Ingram

Download or read book Biomedical Applications of Microprobe Analysis written by Peter Ingram and published by Academic Press. This book was released on 1999-10-29 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. - Up-to-date review of electron microprobe analysis - Detailed descriptions of sample preparation techniques - Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy - Over 100 illustrations with numerous specific applications - Contributions by world-renowned experts in the field - Brief summary of highlights precedes each chapter

Biomedical Electron Microscopy

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Publisher : Elsevier
ISBN 13 : 0080528090
Total Pages : 569 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Biomedical Electron Microscopy by : Arvid B. Maunsbach

Download or read book Biomedical Electron Microscopy written by Arvid B. Maunsbach and published by Elsevier. This book was released on 1998-11-03 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. - Authored by the key leaders in the biological electron microscopy field - Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines - Introduces students on how to read and interpret electron micrographs

Introduction to Analytical Electron Microscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 1475755813
Total Pages : 609 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Introduction to Analytical Electron Microscopy by : John Hren

Download or read book Introduction to Analytical Electron Microscopy written by John Hren and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: The birth of analytical electron microscopy (AEM) is somewhat obscure. Was it the recognition of the power and the development of STEM that signaled its birth? Was AEM born with the attachment of a crystal spectrometer to an otherwise conventional TEM? Or was it born earlier with the first analysis of electron loss spectra? It's not likely that any of these developments alone would have been sufficient and there have been many others (microdiffraction, EDS, microbeam fabrication, etc.) that could equally lay claim to being critical to the establishment of true AEM. It is probably more accurate to simply ascribe the present rapid development to the obvious: a combination of ideas whose time has come. Perhaps it is difficult to trace the birth of AEM simply because it remains a point of contention to even define its true scope. For example, the topics in this book, even though very broad, are still far from a complete description of what many call AEM. When electron beams interact with a solid it is well-known that a bewildering number of possible interactions follow. Analytical electron microscopy attempts to take full qualitative and quantitative advantage of as many of these interactions as possible while still preserving the capability of high resolution imaging. Although we restrict ourselves here to electron transparent films, much of what is described applies to thick specimens as well. Not surprisingly, signals from all possible interactions cannot yet (and probably never will) be attained simultaneously under optimum conditions.

Ultrastructure Techniques for Microorganisms

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Publisher : Springer Science & Business Media
ISBN 13 : 1468451197
Total Pages : 540 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Ultrastructure Techniques for Microorganisms by : H.C. Aldrich

Download or read book Ultrastructure Techniques for Microorganisms written by H.C. Aldrich and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.

Scanning Electron Microscopy and X-Ray Microanalysis

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Publisher : Springer Science & Business Media
ISBN 13 : 1461332737
Total Pages : 679 pages
Book Rating : 4.4/5 (613 download)

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Book Synopsis Scanning Electron Microscopy and X-Ray Microanalysis by : Joseph Goldstein

Download or read book Scanning Electron Microscopy and X-Ray Microanalysis written by Joseph Goldstein and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.

Advances in Imaging and Electron Physics

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Publisher : Elsevier
ISBN 13 : 0080490107
Total Pages : 605 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Advances in Imaging and Electron Physics by : Peter W. Hawkes

Download or read book Advances in Imaging and Electron Physics written by Peter W. Hawkes and published by Elsevier. This book was released on 2004-12-18 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: * A special volume devoted principally to therole of the late Sir Charles Oatley in the development of the scanning electron microscopeings* It contains historical articles and reminiscences by most of the scientists who have worked on the scanning electron microscope in Oatley's laboratory * Emphasizes broad and in depth article collaborations between world-renowned scientists in the field of image and electron physicsAlthough the scanning electron microscope had a prehistory in Germany and the USA, its real champion was Charles Oatley, who launched his projectin the Cambridge University Engineering Department shortly after the end of World War II. A first microscope was built successfully by D. McMullan, oneof the Guest Editors of this volume and a succession of progressively improved instruments followed. One in particular, built by K.C.A. Smith was commissioned specially for the Canadian Pulp and Paper Research Institute for use in their Montreal laboratories. All these efforts culminated in the commercial model built by the Cambridge Instrument Company and marketed in 1965 under the trade name, Stereoscan.Although this story has been told on several occasions, in particular in these Advances, it seemed appropriate, in the centenary year of the birth of Sir Charles Oatley, that more details should be published to celebrate these achievements. This volume is the result. It contains not only historical articles and reminiscences by most of the scientists who have worked on the scanning electron microscope in Oatley's laboratory but also full or partial reproductions of many of the key publications, beginning with McMullan's early paper of 1953 and including Oatley's own "Early history of the scanning electron microscope" (1982). A website has been created, in which supplementary material is collected.This volume is a tribute to a bold pioneering scientist and a vivid record of the creation of the first commercial scanning electron microscopes and of subsequent developments. * A special volume devoted principally to therole of the late Sir Charles Oatley in the development of the scanning electron microscopeings* It contains historical articles and reminiscences by most of the scientists who have worked on the scanning electron microscope in Oatley's laboratory* Emphasizes broad and in depth article collaborations between world-renowned scientists in the field of image and electron physics

Biological Low-Voltage Scanning Electron Microscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 0387729720
Total Pages : 323 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Biological Low-Voltage Scanning Electron Microscopy by : James Pawley

Download or read book Biological Low-Voltage Scanning Electron Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2007-12-03 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

International Review of Cytology

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Publisher : Academic Press
ISBN 13 : 0080586163
Total Pages : 337 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis International Review of Cytology by :

Download or read book International Review of Cytology written by and published by Academic Press. This book was released on 1983-01-07 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Review of Cytology

Biological Field Emission Scanning Electron Microscopy, 2 Volume Set

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Publisher : John Wiley & Sons
ISBN 13 : 1118654064
Total Pages : 741 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by : Roland A. Fleck

Download or read book Biological Field Emission Scanning Electron Microscopy, 2 Volume Set written by Roland A. Fleck and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 741 pages. Available in PDF, EPUB and Kindle. Book excerpt: The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis

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Publisher : Springer Science & Business Media
ISBN 13 : 0387857311
Total Pages : 329 pages
Book Rating : 4.3/5 (878 download)

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Book Synopsis Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis by : Patrick Echlin

Download or read book Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2011-04-14 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.

Plant Nutrition — from Genetic Engineering to Field Practice

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Publisher : Springer Science & Business Media
ISBN 13 : 9401118809
Total Pages : 787 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Plant Nutrition — from Genetic Engineering to Field Practice by : J. Barrow

Download or read book Plant Nutrition — from Genetic Engineering to Field Practice written by J. Barrow and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 787 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Nutrition - From Genetic Engineering to Field Practice, the 12th International Colloquium on Plant Nutrition, is the latest in a series which began in 1954. Early meetings were mainly concerned with the practical problems of soil fertility, with soil assessment, fertilizer requirements and methods of analysis. As the colloquia have progressed, the emphasis has slowly changed. The practical problems are still important, but there is increasing emphasis on plant physiology, plant biochemistry, membrane biochemistry, and even on the chemistry of genes which control the proteins which transfer nutrient ions to the inside of cells. The meetings therefore provide a valuable opportunity for each half of the science of plant nutrition to interact with, and learn from the other half. This volume begins with five papers which review current knowledge in important fields: the rhizosphere, molecular biology, electron microscopy, location and function of elements in vivo, and modelling nutrient responses in the field. These themes are continued in groups of shorter papers which follow. In addition, there are sections on nutrient dynamics and partitioning, diagnostic techniques, plant survival strategies, mycorrhizas, and on nutrients such as P, N, S, K, Ca, Mg, and micronutrients. A large section is devoted specifically to boron - reflecting the considerable current interest in this element. In total there are 177 refereed papers providing both a broad overview and a detailed picture of the latest developments in pure and applied plant nutrition.

X-ray Microanalysis in Biology

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Publisher : Cambridge University Press
ISBN 13 : 9780521415309
Total Pages : 356 pages
Book Rating : 4.4/5 (153 download)

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Book Synopsis X-ray Microanalysis in Biology by : David C. Sigee

Download or read book X-ray Microanalysis in Biology written by David C. Sigee and published by Cambridge University Press. This book was released on 1993-04-22 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes an integrated approach to the use of X-ray microanalysis in biology.

Principles and Practice of Variable Pressure / Environmental Scanning Electron Microscopy (VP-ESEM)

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Publisher : John Wiley & Sons
ISBN 13 : 9780470758748
Total Pages : 234 pages
Book Rating : 4.7/5 (587 download)

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Book Synopsis Principles and Practice of Variable Pressure / Environmental Scanning Electron Microscopy (VP-ESEM) by : Debbie Stokes

Download or read book Principles and Practice of Variable Pressure / Environmental Scanning Electron Microscopy (VP-ESEM) written by Debbie Stokes and published by John Wiley & Sons. This book was released on 2008-11-20 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a simple starting point to VPSEM, especially for new users, technicians and students containing clear, concise explanations Crucially, the principles and applications outlined in this book are completely generic: i.e. applicable to all types of VPSEM, irrespective of manufacturer. Information presented will enable reader to turn principles into practice Published in association with the Royal Microscopical Society (RMS) -www.rms.org.uk

Cumulated Index Medicus

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Publisher :
ISBN 13 :
Total Pages : 928 pages
Book Rating : 4.3/5 (243 download)

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Book Synopsis Cumulated Index Medicus by :

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1980 with total page 928 pages. Available in PDF, EPUB and Kindle. Book excerpt: