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Localization Of Red Clover Necrotic Mosaic Virus Polymerase Proteins At The Endoplasmic Reticulum Of Living Plant Cells
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Book Synopsis Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells by : Katherine Anne Turner
Download or read book Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells written by Katherine Anne Turner and published by . This book was released on 2002 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: plant, remodeling, protein fusions, GFP, ER, viral polymerase, confocal microscopy.
Book Synopsis Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells by :
Download or read book Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The replication of positive strand RNA viruses requires association with host membranes and frequently results in membrane proliferation and rearrangement. The fact that widely divergent virus families share form and function of replication strategies is likely due to common evolutionary origin and suggests that elucidating the reproduction strategies of one virus provides insight to virus life cycles in general. Red clover necrotic mosaic virus (RCNMV) is a positive-strand RNA virus in the Dianthovirus genus, Tombusviridae family. RCNMV encodes N-terminally overlapping proteins of 27 kDa and 88 kDa (p27 and p88). p88 contains motifs characteristic of RNA-dependent RNA polymerases, and while the function of p27 is unclear, it is very likely to be part of the RCNMV replication complex. I have investigated the localization of the RCNMV replicase proteins in plants using green fluorescent protein (GFP) fusions observed in live cells using confocal microscopy. The GFP:p27 and GFP:p88 fusions were expressed in N. benthamiana epidermal cells and protoplasts. GFP:p27 consistently localized to the endoplasmic reticulum (ER) and caused membrane restructuring and proliferation. Fractionation of virus-inoculated leaves demonstrated the association of p27 with isolated ER membranes. Additionally, GFP:p27 co-localized with ER-targeted YFP. GFP:p88 also localized to the ER and co-localized with GFP:p27. In addition, GFP:p27 and GFP:p88 were associated with invaginations of the nuclear membrane. These grooves and channels of ER crossed the nucleus, giving the appearance of fluorescence within the nucleus, while the signal from GFP:p27/p88 was actually associated with ER. GFP:p88 co-localized with GFP:p27 in two expression systems. This and the fact that GFP:p27 localized to the cortical ER, the nuclear envelope, and cytoplasmic ER domains in the presence of viral genomes as well as in the absence of any viral proteins suggests that the two proteins co-localize to the ER during inf.
Book Synopsis Identification and Characterization of the Red Clover Necrotic Mosaic Virus Origin of Assembly by :
Download or read book Identification and Characterization of the Red Clover Necrotic Mosaic Virus Origin of Assembly written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Red clover necrotic mosaic virus (RCNMV) is a single-stranded positive-sense RNA plant virus of the Dianthovirus genus, family Tombusviridae. Each RCNMV virion contains 180 subunits of the 37 kDa capsid protein (CP) forming a non-enveloped, isometric particle of T=3 quasi symmetry, 30-35 nm in diameter. The RCNMV genome consists of two RNAs, RNA-1 and RNA-2. RNA-1 codes for three proteins: i) p88, the RNA-dependent RNA polymerase, ii) p27, the replicase related protein and iii) the CP. RNA-2 is monocistronic and codes for the movement protein (MP). Currently, the RNA complement within RCNMV virions has not been determined. Density gradient centrifugation data has suggested the probability of a single type of particle, whereas the viral RNA profile from virions suggests otherwise. My thesis research has determined the RNA content within RCNMV virions after exposure to either heat or UV-irradiation. Both treatments result in the formation of a stable RNA-1: RNA-2 heterodimer. This leads to the conclusion that RCNMV virions co-package RNA-1 and RNA-2. Upon either treatment, RNA-2 multimers are also observed, suggesting the presence of particles packaging RNA-2 exclusively. These observations suggest that the RCNMV virion population consists of two distinct types of particles having similar densities. Based on the above RNA complement findings, I proceeded to delineate the specific viral sequences that determine the RNA content of an RCNMV virion. During virion assembly, the RCNMV CP must be able to distinguish and package both RNA-1 and RNA-2 while excluding heterologous host RNAs present in the cell. Viral CP subunits recognize specific cognate genomic sequences and/or structures that are unique to each viral genome. These are designated as origin of assembly sequences or packaging signals. To elucidate the assembly mechanism of RCNMV, I searched for the presence of distinct packaging signals on each of the genomic RNAs. Various constructs of RNA-1 and RNA-2 were test.
Book Synopsis The Movement of Red Clover Necrotic Mosaic Virus in Nicotiana Benthamiana Plants Transformed with the Movement Protein by : Andrew Adipongs Vaewhongs
Download or read book The Movement of Red Clover Necrotic Mosaic Virus in Nicotiana Benthamiana Plants Transformed with the Movement Protein written by Andrew Adipongs Vaewhongs and published by . This book was released on 1995 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Movement Protein and Symptomatology of Red Clover Necrotic Mosaic Virus by :
Download or read book The Movement Protein and Symptomatology of Red Clover Necrotic Mosaic Virus written by and published by . This book was released on 1993 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Ribosomal Frameshift Regulation of Viral Polymerase by : Kook-Hyung Kim
Download or read book Ribosomal Frameshift Regulation of Viral Polymerase written by Kook-Hyung Kim and published by . This book was released on 1993 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applied Plant Virology by : L. P. Awasthi
Download or read book Applied Plant Virology written by L. P. Awasthi and published by Academic Press. This book was released on 2020-05-14 with total page 851 pages. Available in PDF, EPUB and Kindle. Book excerpt: Applied Plant Virology: Advances, Detection, and Antiviral Strategies provides an overview on recent developments and applications in the field of plant virology. The book begins with an introduction to important advances in plant virology, but then covers topics including techniques for assay detection and the diagnosis of plant viruses, the purification, isolation and characterization of plant viruses, the architecture of plant viruses, the replication of plant viruses, the physiology of virus-infected hosts, vectors of plant viruses, and the nomenclature and classification of plants. The book also discusses defense strategies by utilizing antiviral agents and management strategies of virus and viroid diseases. With contributions from an international collection of experts, this book presents a practical resource for plant virologists, plant pathologists, horticulturalists, agronomists, biotechnologists, academics and researchers interested in up-to-date technologies and information that advance the field of plant virology. Covers the detection, control and management of plant viruses Discusses antiviral strategies, along with mechanisms of systemic induced resistance to enhance the defense of plants against viruses Provides contributory chapters from expert plant virologists from different parts of the world
Book Synopsis Plant RNA Viruses by : Rajarshi Kumar Gaur
Download or read book Plant RNA Viruses written by Rajarshi Kumar Gaur and published by Elsevier. This book was released on 2023-03-22 with total page 668 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant RNA Viruses: Molecular Pathogenesis and Management provides wide-ranging coverage on the recognition and signaling events between plants and RNA viruses. The book examines the molecular biology of signaling, host-virus interaction, RNA virus diversity, and how plants and cellular pathogens interact. Sections cover Virus Diversity and Diagnosis, Virus-Host Interactions and Virus Management. Specific chapters discuss classification and nomenclature of viruses, detail the molecular characteristics of viral genomes, highlight the viral manipulation of cellular key regulatory systems for successful virus infection, and discuss the movement of plant viruses into plant cells. Additional topics include RNA plant viruses and host interaction, detection and diversity of plant RNA viruses, and strategies for combating and management of plant viruses. With contributions from an international group of experts, the book is a comprehensive reference for those in research, academia, industry and anybody engaging in the study of plant viruses at the molecular level. Provides an overview of virus genome and nomenclature Covers plant virus biodiversity and ecology, diversity mechanisms and opportunities Examines Plant Host-Virus Interaction Discusses virus-vector interaction and transmission Offers strategies for virus management, both traditional and modern
Book Synopsis Plant cell endomembrane dynamics and specialization by : Emily R Larson
Download or read book Plant cell endomembrane dynamics and specialization written by Emily R Larson and published by Frontiers Media SA. This book was released on 2023-11-09 with total page 119 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 2002 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in Plant-Hemipteran Interactions by : Jyoti Shah
Download or read book Advances in Plant-Hemipteran Interactions written by Jyoti Shah and published by Frontiers Media SA. This book was released on 2017-11-08 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hemipterans encompass a large group of insect pests of plants that utilize mouthparts which are modified for piercing and consuming fluids from plants. In addition, hemipterans vector viral and bacterial diseases of plants. This book brings together a set of reviews and research papers that showcase the the range of activities being undertaken to advance our understanding of the multi-organismal interaction between plant, hemipterans and microbes.
Book Synopsis Multiplication of RNA Plant Viruses by : Chundi L. Mandahar
Download or read book Multiplication of RNA Plant Viruses written by Chundi L. Mandahar and published by Springer Science & Business Media. This book was released on 2006-09-05 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical studies on plant virus RNA replication have advanced considerably since 2000, primarily because of new genetic, molecular, biochemical, and enzymatic studies. This book generates understanding of multiplication of plus-sense RNA plant viruses, especially at molecular level. Certain virus-encoded essential proteins, nucleotide sequence motifs, and RNA secondary structures are central to virus RNA replication, which has a number of stages. Each is a complex phenomenon requiring specific factors and conditions.
Book Synopsis Natural and Engineered Resistance to Plant Viruses by :
Download or read book Natural and Engineered Resistance to Plant Viruses written by and published by Academic Press. This book was released on 2009-11-03 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viruses are a huge threat to agriculture. In the past, viruses used to be controlled using conventional methods, such as crop rotation and destruction of the infected plants, but now there are more novel ways to control them. This volume focuses on natural and engineered virus resistance, the two major strategies used for crop protection. Contributions from leading authorities Informs and updates on all the latest developments in the field
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1996 with total page 2376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Virology written by Roger Hull and published by Academic Press. This book was released on 2013-10-31 with total page 1119 pages. Available in PDF, EPUB and Kindle. Book excerpt: The seminal text Plant Virology is now in its fifth edition. It has been 10 years since the publication of the fourth edition, during which there has been an explosion of conceptual and factual advances. The fifth edition of Plant Virology updates and revises many details of the previous edition while retaining the important earlier results that constitute the field's conceptual foundation. Revamped art, along with fully updated references and increased focus on molecular biology, transgenic resistance, aphid transmission, and new, cutting-edge topics, bring the volume up to date and maintain its value as an essential reference for researchers and students in the field. Thumbnail sketches of each genera and family groups Genome maps of all genera for which they are known Genetic engineered resistance strategies for virus disease control Latest understanding of virus interactions with plants, including gene silencing Interactions between viruses and insect, fungal, and nematode vectors Contains over 300 full-color illustrations
Book Synopsis Plasmodesmata by : Aart J.E. van Bel
Download or read book Plasmodesmata written by Aart J.E. van Bel and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plasmodesmata are minuscule plasma corridors between plant cells which are of paramount importance for transport, communication and signalling between cells. These nano-channels are responsible for the integrated action of cells within tissues and for the subdivision of the plant body into working symplast units. This book updates the wealth of new information in this rapidly expanding field. Reputed workers in the field discuss major techniques in plasmodesmatal research and describe recent discoveries on the ultrastructure, the functioning and the role of plasmodesmata in intracellular transport and communication, in cell differentiation, plant development and virus translocation.
Book Synopsis Plant Viruses: From Ecology to Control by : Jesús Navas Castillo
Download or read book Plant Viruses: From Ecology to Control written by Jesús Navas Castillo and published by . This book was released on 2021 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant viruses cause many of the most important diseases threatening crops worldwide. Over the last quarter of a century, an increasing number of plant viruses have emerged in various parts of the world, especially in the tropics and subtropics. As is generally observed for plant viruses, most of the emerging viruses are transmitted horizontally by biological vectors, mainly insects. Reverse genetics using infectious clones--available for many plant viruses--has been used for identification of viral determinants involved in virus-host and virus-vector interactions. Although many studies have identified a number of factors involved in disease development and transmission, the precise mechanisms are unknown for most of the virus-plant-vector combinations. In most cases, the diverse outcomes resulting from virus-virus interactions are poorly understood. Although significant advances have been made towards understand the mechanisms involved in plant resistance to viruses, we are far from being able to apply this knowledge to protect cultivated plants from the all viral threats.The aim of this Special Issue was to provide a platform for researchers interested in plant virology to share their recent results. To achieve this, we invited the plant virology community to submit research articles, short communications and reviews related to the various aspects of plant virology: ecology, virus-plant host interactions, virus-vector interactions, virus-virus interactions, and control strategies. This issue contains some of the best current research in plant virology.
