Liposome Methods And Protocols

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Liposomes

Author : Gerard G.M. D'Souza
Publisher : Springer Nature
ISBN 13 : 1071629549
Total Pages : 301 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Liposomes by : Gerard G.M. D'Souza

Download or read book Liposomes written by Gerard G.M. D'Souza and published by Springer Nature. This book was released on 2023-02-13 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.

Liposome Methods and Protocols

Author : Subhash C. Basu
Publisher : Humana Press
ISBN 13 : 9780896038455
Total Pages : 252 pages
Book Rating : 4.0/5 (384 download)

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Book Synopsis Liposome Methods and Protocols by : Subhash C. Basu

Download or read book Liposome Methods and Protocols written by Subhash C. Basu and published by Humana Press. This book was released on 2002-05-15 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.

Liposomes

Author : Volkmar Weissig
Publisher :
ISBN 13 : 9781607614470
Total Pages : pages
Book Rating : 4.6/5 (144 download)

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Book Synopsis Liposomes by : Volkmar Weissig

Download or read book Liposomes written by Volkmar Weissig and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane.?Volume 1: Pharmaceutical Nanocarriers focuses on the state-of-the-art aspects of developing liposome-based nanoscale drug delivery techniques, delving into the depths of the emerging field of pharmaceutical and biomedical nanotechnology. As a volume in the highly successful Methods in Molecular BiologyTM series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Liposomes: Methods and Protocols promises to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol.

Methods in Molecular Biology: Liposome methods and protocols

Author : John M. Walker
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (84 download)

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Book Synopsis Methods in Molecular Biology: Liposome methods and protocols by : John M. Walker

Download or read book Methods in Molecular Biology: Liposome methods and protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Liposomes

Author : Volkmar Weissig
Publisher :
ISBN 13 : 9781603273602
Total Pages : pages
Book Rating : 4.2/5 (736 download)

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Book Synopsis Liposomes by : Volkmar Weissig

Download or read book Liposomes written by Volkmar Weissig and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Liposome Methods And Protocols

Author : Subhash C. Basu
Publisher :
ISBN 13 : 9788184891485
Total Pages : 264 pages
Book Rating : 4.8/5 (914 download)

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Book Synopsis Liposome Methods And Protocols by : Subhash C. Basu

Download or read book Liposome Methods And Protocols written by Subhash C. Basu and published by . This book was released on 2009-02-01 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Topics I Physical Properties 1. Preparation, Isolation, And Characterisation Of Liposomes Containing Natural And Synthetic Lipids 2. Preparation And Use Of Liposomes For The Study Of Sphingolipid Segregation In Membrane Model Systems II Liposome Fusion/Modulation 3. Peptide-Induced Fusion Of Liposomes 4. Liposomes: Applications In Protein Lipid Interaction Studies 5. Lipids In Viral Fusion III Application Of Liposomes 6. Liposome-Mediated, Fluorescence-Based Studies Of Sphingolipid Metabolism In Intact Cells 7. Micelles And Liposomes In Metabolic Enzyme And Glycolipid Glycosyl Transferase Assays

Liposome Methods and Protocols

Author : Subhash C. Basu
Publisher : Springer Science & Business Media
ISBN 13 : 1592591752
Total Pages : 250 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Liposome Methods and Protocols by : Subhash C. Basu

Download or read book Liposome Methods and Protocols written by Subhash C. Basu and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.

Liposome Methods and Protocols

Author : Subhash C. Basu
Publisher : Humana Press
ISBN 13 : 9780896038455
Total Pages : 252 pages
Book Rating : 4.0/5 (384 download)

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Book Synopsis Liposome Methods and Protocols by : Subhash C. Basu

Lipidomics

Author : Sanjoy K. Bhattacharya
Publisher : Springer Nature
ISBN 13 : 1071629662
Total Pages : 375 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Lipidomics by : Sanjoy K. Bhattacharya

Download or read book Lipidomics written by Sanjoy K. Bhattacharya and published by Springer Nature. This book was released on 2023-01-18 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.

Liposomes

Author : Gerard G. M. D'Souza
Publisher :
ISBN 13 : 9781493965915
Total Pages : 285 pages
Book Rating : 4.9/5 (659 download)

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Book Synopsis Liposomes by : Gerard G. M. D'Souza

Download or read book Liposomes written by Gerard G. M. D'Souza and published by . This book was released on 2016 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry. .

Liposomes

Author : Volkmar Weissig
Publisher : Humana Press
ISBN 13 : 9781603273596
Total Pages : 588 pages
Book Rating : 4.2/5 (735 download)

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Book Synopsis Liposomes by : Volkmar Weissig

Download or read book Liposomes written by Volkmar Weissig and published by Humana Press. This book was released on 2009-12-18 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that “the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer” [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.

Phosphoinositides

Author : Roberto Botelho
Publisher :
ISBN 13 : 9781071611425
Total Pages : 239 pages
Book Rating : 4.6/5 (114 download)

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Book Synopsis Phosphoinositides by : Roberto Botelho

Download or read book Phosphoinositides written by Roberto Botelho and published by . This book was released on 2021 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.

Liposome-Based Drug Delivery Systems

Author : Wan-Liang Lu
Publisher : Springer
ISBN 13 : 9783662493182
Total Pages : 0 pages
Book Rating : 4.4/5 (931 download)

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Book Synopsis Liposome-Based Drug Delivery Systems by : Wan-Liang Lu

Download or read book Liposome-Based Drug Delivery Systems written by Wan-Liang Lu and published by Springer. This book was released on 2021-07-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes the protocols for fabrication of liposomal drug delivery system, and consists of two parts. The first part emphasizes the basic protocols and concepts for fabrication of drug-loaded liposomes. For new investigators, laying the groundwork is the first step. This part focuses on the fabrication details in liposomes formulations, which are the so-called small tricks while usually not disclosed in mostly published research articles. However, these processing details are crucial for the preparation of liposomes. And the second part focuses on the strategies in modified and/or functionalized liposomes to adapt to pathophysiological environment, as well as their application in treating diseases. As new analytical and synthetic technologies become available, and improved understanding of pathophysiology, various functionalized drug liposomes are developing to fit the requirements of different therapeutic purposes, exhibiting a broad range of applications. Accordingly, the objectives of this part are aimed at deeply unearthing the formulation of drug-loaded liposomes, describing the detailed operation of liposomal formulation, and further demonstrating their potential applications. Therefore, this volume shows the features of experimental report or protocol for proving a guide to scientists, research students, and young investigators in pharmaceutical enterprises.

Liposomes: A Practical Approach

Author : V. P. Torchilin
Publisher : Oxford University Press
ISBN 13 : 9780199636549
Total Pages : 428 pages
Book Rating : 4.6/5 (365 download)

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Book Synopsis Liposomes: A Practical Approach by : V. P. Torchilin

Download or read book Liposomes: A Practical Approach written by V. P. Torchilin and published by Oxford University Press. This book was released on 2003-06-05 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an up-to-date and unique collection of experimental protocols from an area of pharmaceutical research that is essential for the development of new, highly specific drugs as well as for the exploration of completely new therapeutic approaches to disease treatments.

Protein Lipidation

Author : Maurine E. Linder
Publisher : Humana
ISBN 13 : 9781493995318
Total Pages : 327 pages
Book Rating : 4.9/5 (953 download)

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Book Synopsis Protein Lipidation by : Maurine E. Linder

Download or read book Protein Lipidation written by Maurine E. Linder and published by Humana. This book was released on 2019-06-01 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Lipidation: Methods and Protocols is a valuable resource for experts in the field and for investigators who encounter protein lipidation through their research on a particular cellular process or favorite protein.

Liposome Protocols

Author : Ashok K. Shukla
Publisher :
ISBN 13 : 9780896037151
Total Pages : pages
Book Rating : 4.0/5 (371 download)

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Book Synopsis Liposome Protocols by : Ashok K. Shukla

Download or read book Liposome Protocols written by Ashok K. Shukla and published by . This book was released on 1999-04-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Enzyme Stabilization and Immobilization

Author : Shelley D. Minteer
Publisher : Humana
ISBN 13 : 9781493964970
Total Pages : 0 pages
Book Rating : 4.9/5 (649 download)

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Book Synopsis Enzyme Stabilization and Immobilization by : Shelley D. Minteer

Download or read book Enzyme Stabilization and Immobilization written by Shelley D. Minteer and published by Humana. This book was released on 2016-10-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).