Laboratory Protocols In Applied Life Sciences

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Laboratory Protocols in Applied Life Sciences

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Author : Prakash Singh Bisen
Publisher : CRC Press
ISBN 13 : 1466553146
Total Pages : 1812 pages
Book Rating : 4.4/5 (665 download)

Book Synopsis Laboratory Protocols in Applied Life Sciences by : Prakash Singh Bisen

Download or read book Laboratory Protocols in Applied Life Sciences written by Prakash Singh Bisen and published by CRC Press. This book was released on 2014-02-26 with total page 1812 pages. Available in PDF, EPUB and Kindle. Book excerpt: As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.

LABORATORY PROTOCOLS IN APPLIED LIFE SCIENCES.

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Author : PRAKASH SINGH. BISEN
Publisher :
ISBN 13 : 9781138071957
Total Pages : pages
Book Rating : 4.0/5 (719 download)

Book Synopsis LABORATORY PROTOCOLS IN APPLIED LIFE SCIENCES. by : PRAKASH SINGH. BISEN

Download or read book LABORATORY PROTOCOLS IN APPLIED LIFE SCIENCES. written by PRAKASH SINGH. BISEN and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Current Protocols Essential Laboratory Techniques

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Author : Sean R. Gallagher
Publisher : John Wiley & Sons
ISBN 13 : 047094241X
Total Pages : 679 pages
Book Rating : 4.4/5 (79 download)

Book Synopsis Current Protocols Essential Laboratory Techniques by : Sean R. Gallagher

Download or read book Current Protocols Essential Laboratory Techniques written by Sean R. Gallagher and published by John Wiley & Sons. This book was released on 2012-03-19 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Laboratory Protocols in Fungal Biology

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Author : Vijai Kumar Gupta
Publisher : Springer Science & Business Media
ISBN 13 : 1461423562
Total Pages : 608 pages
Book Rating : 4.4/5 (614 download)

Book Synopsis Laboratory Protocols in Fungal Biology by : Vijai Kumar Gupta

Download or read book Laboratory Protocols in Fungal Biology written by Vijai Kumar Gupta and published by Springer Science & Business Media. This book was released on 2012-12-09 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology

Introduction to Instrumentation in Life Sciences

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Author : Prakash Singh Bisen
Publisher : CRC Press
ISBN 13 : 1466512407
Total Pages : 387 pages
Book Rating : 4.4/5 (665 download)

Book Synopsis Introduction to Instrumentation in Life Sciences by : Prakash Singh Bisen

Download or read book Introduction to Instrumentation in Life Sciences written by Prakash Singh Bisen and published by CRC Press. This book was released on 2012-09-26 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: Instrumentation is central to the study of physiology and genetics in living organisms, especially at the molecular level. Numerous techniques have been developed to address this in various biological disciplines, creating a need to understand the physical principles involved in the operation of research instruments and the parameters required in using them. Introduction to Instrumentation in Life Sciences fills this need by addressing different aspects of tools that hold the keys to cutting-edge research and innovative applications, from basic techniques to advanced instrumentation. The text describes all topics so even beginners can easily understand the theoretical and practical aspects. Comprehensive chapters encompass well-defined methodology that describes the instruments and their corresponding applications in different scientific fields. The book covers optical and electron microscopy; micrometry, especially in microbial taxonomy; pH meters and oxygen electrodes; chromatography for separation and purification of products from complex mixtures; spectroscopic and spectrophotometric techniques to determine structure and function of biomolecules; preparative and analytical centrifugation; electrophoretic techniques; x-ray microanalysis including crystallography; applications of radioactivity, including autoradiography and radioimmunoassays; and fermentation technology and subsequent separation of products of interest. The book is designed to serve a wide range of students and researchers in diversified fields of life sciences: pharmacy, biotechnology, microbiology, biochemistry, and environmental sciences. It introduces different aspects of basic experimental methods and instrumentation. The book is unique in its broad subject coverage, incorporating fundamental techniques as well as applications of modern molecular and proteomic tools that are the basis for state-of-the-art research. The text emphasizes techniques encountered both in practical classes and in high-throughput environments used in modern industry. As a further aid to students, the authors provide well-illustrated diagrams to explain the principles and theories behind the instruments described.

Biology Laboratory Protocols

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Author : Christopher Panaretos
Publisher : Independently Published
ISBN 13 : 9781075696831
Total Pages : 31 pages
Book Rating : 4.6/5 (968 download)

Book Synopsis Biology Laboratory Protocols by : Christopher Panaretos

Download or read book Biology Laboratory Protocols written by Christopher Panaretos and published by Independently Published. This book was released on 2019-06-23 with total page 31 pages. Available in PDF, EPUB and Kindle. Book excerpt: This short, 30 page book gives a brief and generalized overview of the most common concepts in biology laboratory research. Standard laboratory protocols utilize these concepts in a modular fashion. Understanding this modular nature enables you to design custom protocols to collect data from arbitrary biological systems.

Basic Science Methods for Clinical Researchers

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Author : Morteza Jalali
Publisher : Academic Press
ISBN 13 : 012803078X
Total Pages : 382 pages
Book Rating : 4.1/5 (28 download)

Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali

Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Laboratory Life

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Author : Bruno Latour
Publisher : Princeton University Press
ISBN 13 : 1400820413
Total Pages : 296 pages
Book Rating : 4.4/5 (8 download)

Book Synopsis Laboratory Life by : Bruno Latour

Download or read book Laboratory Life written by Bruno Latour and published by Princeton University Press. This book was released on 2013-04-04 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly original work presents laboratory science in a deliberately skeptical way: as an anthropological approach to the culture of the scientist. Drawing on recent work in literary criticism, the authors study how the social world of the laboratory produces papers and other "texts,"' and how the scientific vision of reality becomes that set of statements considered, for the time being, too expensive to change. The book is based on field work done by Bruno Latour in Roger Guillemin's laboratory at the Salk Institute and provides an important link between the sociology of modern sciences and laboratory studies in the history of science.

Life Sciences and Related Fields

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Author : International Union of Microbiological Societies
Publisher : National Academies Press
ISBN 13 : 0309210712
Total Pages : 164 pages
Book Rating : 4.3/5 (92 download)

Book Synopsis Life Sciences and Related Fields by : International Union of Microbiological Societies

Download or read book Life Sciences and Related Fields written by International Union of Microbiological Societies and published by National Academies Press. This book was released on 2012-01-02 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the last decade, national and international scientific organizations have become increasingly engaged in considering how to respond to the biosecurity implications of developments in the life sciences and in assessing trends in science and technology (S&T) relevant to biological and chemical weapons nonproliferation. The latest example is an international workshop, Trends in Science and Technology Relevant to the Biological Weapons Convention, held October 31 - November 3, 2010 at the Institute of Biophysics of the Chinese Academy of Sciences in Beijing. Life Sciences and Related Fields summarizes the workshop, plenary, and breakout discussion sessions held during this convention. Given the immense diversity of current research and development, the report is only able to provide an overview of the areas of science and technology the committee believes are potentially relevant to the future of the Biological and Toxic Weapons Convention (BWC), although there is an effort to identify areas that seemed particularly ripe for further exploration and analysis. The report offers findings and conclusions organized around three fundamental and frequently cited trends in S&T that affect the scope and operation of the convention: The rapid pace of change in the life sciences and related fields; The increasing diffusion of life sciences research capacity and its applications, both internationally and beyond traditional research institutions; and The extent to which additional scientific and technical disciplines beyond biology are increasingly involved in life sciences research. The report does not make recommendations about policy options to respond to the implications of the identified trends. The choice of such responses rests with the 164 States Parties to the Convention, who must take into account multiple factors beyond the project's focus on the state of the science.

Protocols used in Molecular Biology

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Author : Sandeep Singh
Publisher : Bentham Science Publishers
ISBN 13 : 981143929X
Total Pages : 186 pages
Book Rating : 4.8/5 (114 download)

Book Synopsis Protocols used in Molecular Biology by : Sandeep Singh

Download or read book Protocols used in Molecular Biology written by Sandeep Singh and published by Bentham Science Publishers. This book was released on 2020-01-23 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Phage Display

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Author : Carlos F. Barbas
Publisher : CSHL Press
ISBN 13 : 0879697407
Total Pages : 724 pages
Book Rating : 4.8/5 (796 download)

Book Synopsis Phage Display by : Carlos F. Barbas

Download or read book Phage Display written by Carlos F. Barbas and published by CSHL Press. This book was released on 2001 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.

Flow Cytometry

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Author : Alice Longobardi Givan
Publisher : John Wiley & Sons
ISBN 13 : 1118688392
Total Pages : 309 pages
Book Rating : 4.1/5 (186 download)

Book Synopsis Flow Cytometry by : Alice Longobardi Givan

Download or read book Flow Cytometry written by Alice Longobardi Givan and published by John Wiley & Sons. This book was released on 2013-04-10 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Analytical Techniques in Biosciences

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Author : Chukwuebuka Egbuna
Publisher : Academic Press
ISBN 13 : 0128227990
Total Pages : 302 pages
Book Rating : 4.1/5 (282 download)

Book Synopsis Analytical Techniques in Biosciences by : Chukwuebuka Egbuna

Download or read book Analytical Techniques in Biosciences written by Chukwuebuka Egbuna and published by Academic Press. This book was released on 2021-10-21 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. Presents basic analytical protocols and sample-preparation guidelines Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research Presents biostatistical tools and methods and basic computational models in biosciences

Laboratory Investigations in Molecular Biology

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Author : Steven A. Williams
Publisher : Jones & Bartlett Learning
ISBN 13 : 9780763733292
Total Pages : 272 pages
Book Rating : 4.7/5 (332 download)

Book Synopsis Laboratory Investigations in Molecular Biology by : Steven A. Williams

Download or read book Laboratory Investigations in Molecular Biology written by Steven A. Williams and published by Jones & Bartlett Learning. This book was released on 2007 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Investigations in Molecular Biology presents well-tested protocols in molecular biology that are commonly used in currently active research labs. It is an ideal laboratory manual for college level courses in molecular biology. Because of the modular organization of the manual, laboratory courses can be assembled that would be ideal for science professionals, graduate students, undergraduate students and even advanced high school students in AP courses. The manual is also intended to be useful as a laboratory "bench reference". The experiments are designed to guide students through realistic research projects and to provide students with instruction in methods and approaches that can be immediately translated into research projects conducted in modern research laboratories. Although these experiments have been conducted and optimized over 20 years of teaching the New England Biolabs Molecular Biology Summer Workshops, they are real research projects, not "canned" experiments. Based on extensive teaching experience using these protocols, the authors have found that conducting these experiments as described in these protocols serves to effectively instruct students and science professions in the basic methods of molecular biology. An additional unique feature is that the protocols described in the manual are accompanied by available reagent kits that provide quality-tested, pre-packaged reagents to ensure the successful application of these protocols in a laboratory course setting.

Metastasis Research Protocols

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Author : Susan A. Brooks
Publisher : Springer Science & Business Media
ISBN 13 : 159259137X
Total Pages : 310 pages
Book Rating : 4.5/5 (925 download)

Book Synopsis Metastasis Research Protocols by : Susan A. Brooks

Download or read book Metastasis Research Protocols written by Susan A. Brooks and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Volume I, Analysis of Cells and Tissues, we presented a range of protocols aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research protocols, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systems in turn must of course be put into perspective relative to their roles in entire living organisms.

PCR Protocols

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Author : Bruce A. White
Publisher : Springer Science & Business Media
ISBN 13 : 1592595022
Total Pages : 397 pages
Book Rating : 4.5/5 (925 download)

Book Synopsis PCR Protocols by : Bruce A. White

Download or read book PCR Protocols written by Bruce A. White and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.

Laboratory protocols: CIMMYT Applied genetic engineering laboratory

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Author :
Publisher : CIMMYT
ISBN 13 : 9706480412
Total Pages : 144 pages
Book Rating : 4.7/5 (64 download)

Book Synopsis Laboratory protocols: CIMMYT Applied genetic engineering laboratory by :

Download or read book Laboratory protocols: CIMMYT Applied genetic engineering laboratory written by and published by CIMMYT. This book was released on 1999 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: