Protein Liquid Chromatography

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Publisher : Elsevier
ISBN 13 : 9780444502117
Total Pages : 978 pages
Book Rating : 4.5/5 (21 download)

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Book Synopsis Protein Liquid Chromatography by : M. Kastner

Download or read book Protein Liquid Chromatography written by M. Kastner and published by Elsevier. This book was released on 2000 with total page 978 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Monolithic Materials

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Publisher : Elsevier
ISBN 13 : 0080536972
Total Pages : 800 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Monolithic Materials by : F. Svec

Download or read book Monolithic Materials written by F. Svec and published by Elsevier. This book was released on 2003-04-29 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, monolithic materials in the shape of discs, stacked layers, rolled sheets, sponges, irregular chunks, tubes, and cylinders have all been successfully demonstrated. These formats were prepared from a wide variety of materials including natural polymers such as cellulose, synthetic polymers that involved porous styrene-, methacrylate-, and acrylamide-based polymers, and inorganic materials, mainly silica. Each approach is interesting from the point of view of both preparation and application.Although the current papers and patents concerned with monolithic separation media are quite numerous, the information is scattered throughout a vast number of journals. This book therefore fills the gap in the market for a comprehensive reference book on this subject.Monolithic materials concerns all of the current formats of monolithic materials and provides an integrated view of this novel format of separation media. Since the flow pattern in monolithic devices is different from that in packed beds, the hydrodynamics of the system and mass transport differ considerably from those derived for packed columns. Therefore, this book presents contributions concerned with both flow and mass transfer in the monolithic materials. A significant proportion of the book is devoted to the applications of monolithic materials. It also provides the reader with valuable information about the sources of the specific materials, their properties, and potential applications.·Monolithic materials are currently very popular within several scientific areas such as chromatography, optics, catalysis, diagnostics, genomics, proteomics, and microfluidics.·Provides valuable information about the sources of the specific materials, their properties, and potential applications.·Chapters written by leading experts in the area.

Chromatography of Antibiotics

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Publisher : Elsevier
ISBN 13 : 0080858295
Total Pages : 523 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Chromatography of Antibiotics by : M.J. Weinstein

Download or read book Chromatography of Antibiotics written by M.J. Weinstein and published by Elsevier. This book was released on 2000-04-01 with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography of Antibiotics

Sample Preparation in Chromatography

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Publisher : Elsevier
ISBN 13 : 0080540708
Total Pages : 949 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Sample Preparation in Chromatography by : S.C. Moldoveanu

Download or read book Sample Preparation in Chromatography written by S.C. Moldoveanu and published by Elsevier. This book was released on 2002-05-08 with total page 949 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sample preparation is an essential step in many analyses. This book approaches the topic of sample preparation in chromatography in a methodical way, viewing it as a logical connection between sample collection and analytical chromatography. Providing a guide for choosing the appropriate sample preparation for a given analysis, this book describes various ways to process the sample, explaining the principle, discussing the advantages and disadvantages, describing the applicability to different types of samples, and showing the fitness to specific chromatographic determinations. The first part of the book contains an overview of sample preparation showing its relation to sample collection and to the core chromatographic analysis. The second part covers procedures that do not use chemical modifications of the analyte and includes methods for sample dissolution, concentration and cleanup designed mainly for modifying the initial matrix of the sample. This part starts with conventional separations such as filtration and distillation and finishes with more advanced techniques such as solid phase extraction and electroseparations. The third part gives a description of the chemical modifications that can be performed on a sample either for fractionation purposes or to improve a specific property of the analyte. This part includes derivatizations, polymer chemical degradations, and pyrolysis.

Chromatography

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Publisher : Elsevier
ISBN 13 : 0080472249
Total Pages : 585 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Chromatography by : E. Heftmann

Download or read book Chromatography written by E. Heftmann and published by Elsevier. This book was released on 2004-04-16 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of future importance, such as microfluidics and computer resources. Under his experienced guidance, authorities in each field have contributed their practical experience to an integrated treatment of modern micro analysis. In Part A the theoretical basis of individual separation methods is explained and the technical aspects are illustrated. It includes the theory of gas and liquid chromatography as well as specific chromatographic techniques, such as size-exclusion, planar, ion, and affinity chromatography as well as various electrokinetic separation techniques. Microfluidics are covered for the first time and useful sources of analytical instruments are listed and evaluated.1. Each chapter written by an authority2. Thorough treatment of the theoretical basis of separation methods3. Practical guide for performing analyses

Ion Chromatography

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Publisher : Elsevier
ISBN 13 : 0080858538
Total Pages : 799 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Ion Chromatography by :

Download or read book Ion Chromatography written by and published by Elsevier. This book was released on 1990-07-16 with total page 799 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ion Chromatography

Journal of Chromatography

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Publisher :
ISBN 13 :
Total Pages : 926 pages
Book Rating : 4.6/5 (238 download)

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Book Synopsis Journal of Chromatography by :

Download or read book Journal of Chromatography written by and published by . This book was released on 2003 with total page 926 pages. Available in PDF, EPUB and Kindle. Book excerpt:

75 Years of Chromatography

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Publisher : Elsevier
ISBN 13 : 0080858171
Total Pages : 517 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis 75 Years of Chromatography by :

Download or read book 75 Years of Chromatography written by and published by Elsevier. This book was released on 2011-08-26 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: 75 Years of Chromatography

Stationary Phases in Gas Chromatography

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Publisher : Elsevier
ISBN 13 : 0080858554
Total Pages : 425 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Stationary Phases in Gas Chromatography by : H. Rotzsche

Download or read book Stationary Phases in Gas Chromatography written by H. Rotzsche and published by Elsevier. This book was released on 1991-08-21 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: The primary aim of this volume is to make the chemist familiar with the numerous stationary phases and column types, with their advantages and disadvantages, to help in the selection of the most suitable phase for the type of analytes under study. The book also provides detailed information on the chemical structure, physico-chemical behaviour, experimental applicability, physical data of liquid and solid stationary phases and solid supports. Such data were previously scattered throughout the literature. To understand the processes occurring in the separation column and to offer a manual both to the beginner and to the experienced chromatographer, one chapter is devoted to the basic theoretical aspects. Further, as the effectiveness of the stationary phase can only be considered in relation to the column type, a chapter on different column types and the arrangement of the stationary phase within the column is included.The secondary aim of this book is to stimulate the development of new and improved standardized stationary phases and columns, in order to improve the reproducibility of separations, as well as the range of applications.

Extraction Chromatography

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Publisher : Elsevier
ISBN 13 : 0080858023
Total Pages : 585 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Extraction Chromatography by :

Download or read book Extraction Chromatography written by and published by Elsevier. This book was released on 1975-06-01 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extraction Chromatography

Chemical Derivatization in Gas Chromatography

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Publisher : Elsevier
ISBN 13 : 0080858201
Total Pages : 247 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Chemical Derivatization in Gas Chromatography by : J. Drozd

Download or read book Chemical Derivatization in Gas Chromatography written by J. Drozd and published by Elsevier. This book was released on 1986-07-01 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Derivatization in Gas Chromatography

Gas Chromatography in Air Pollution Analysis

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Publisher : Elsevier
ISBN 13 : 0080858562
Total Pages : 225 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Gas Chromatography in Air Pollution Analysis by : V.G. Berezkin

Download or read book Gas Chromatography in Air Pollution Analysis written by V.G. Berezkin and published by Elsevier. This book was released on 1991-04-25 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: Air pollution determination is one of the most important fields of gas chromatography application in practice. This book provides a systematic description of the main stages of air pollution determination, ranging from sampling problems to the quantitative estimation of the acquired data. Special attention is paid to the problem of gas, vapor, spray and solid particles extraction from air. The main methods of sampling procedure, namely, container utilization, cryogenic concentration, absorption, adsorption, chemisorption and filter usage, and successive impurities extraction are also handled. Sorption theory and the problems of sorption and desorption efficiency for hazardous impurities being extracted from traps with sorbents are discussed in detail. The practical utilization of different sorbents (silica, activated carbon, polymers etc.) to carry out sampling procedures for 200 main pollutants with known TLV (USSR and USA) is also considered. This highly informative book, reflecting several insufficiently known techniques as well as the experience of both western and Soviet researchers, should be of interest to both beginners and skilled researchers.

Retention and Selectivity in Liquid Chromatography

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Publisher : Elsevier
ISBN 13 : 0080858651
Total Pages : 479 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Retention and Selectivity in Liquid Chromatography by : R.M. Smith

Download or read book Retention and Selectivity in Liquid Chromatography written by R.M. Smith and published by Elsevier. This book was released on 1995-01-13 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together a number of studies which examine the ways in which the retention and selectivity of separations in high-performance liquid chromatography are dependent on the chemical structure of the analytes and the properties of the stationary and mobile phases. Although previous authors have described the optimisation of separations by alteration of the mobile phase, little emphasis has previously been reported of the influence of the structure and properties of the analyte.The initial chapters describe methods based on retention index group increments and log P increments for the prediction of the retention of analytes and the ways in which these factors are influenced by mobile phases and intramolecular interactions. The values of a wide range of group increments in different eluents are tabulated.Different scales of retention indices in liquid chromatography are described for the comparison of separations, the identification of analytes and the comparison of stationary phases. Applications of these methods in the pharmaceutical, toxicology, forensic, metabolism, environmental, food and other fields are reviewed. The effects of different mobile phases on the selectivity of the retention indices are reported. A compilation of sources of reported retention index values are given.Methods for the comparison of stationary phases based on the interactions of different analytes are covered, including lipophilic and polar indices, shape selectivity comparisons, their application to novel stationary phases, and chemometric methods for column comparisons.

Analytical Artifacts

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Publisher : Elsevier
ISBN 13 : 008085849X
Total Pages : 1059 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Analytical Artifacts by : B.S. Middleditch

Download or read book Analytical Artifacts written by B.S. Middleditch and published by Elsevier. This book was released on 1989-01-01 with total page 1059 pages. Available in PDF, EPUB and Kindle. Book excerpt: This encyclopaedic catalogue of the pitfalls and problems that all analysts encounter in their work is destined to spend more time on the analyst's workbench than on a library shelf. The author has dedicated the book to ``the innumerable scientists who made mistakes, used impure chemicals and solvents, suffered the consequences of unanticipated side-reactions, and were otherwise exposed to mayhem yet were not too embarrassed to publish their findings''. Traditionally, the mass spectroscopist or gas chromatographer learnt his trade by participating in a 4-6 year apprenticeship as graduate student and post-doctoral researcher. Generally, no formal training was provided on the things that go wrong, but this information was accumulated by sharing in the experiences of colleagues. Nowadays, many novice scientists simply purchase a computerized instrument, plug it in, and use it. Much time can be wasted in studying and resolving problems due to artifacts and there is also a strong possibility that artifacts will not be recognized as such. For example, most analysts realize that they should use glass rather than plastic containers; but few of them would anticipate the possibility of plasticizer residues on glassware washed using detergent from a plastic bottle.This book is an easy-to-use compendium of problems encountered when using various commonly used analytical techniques. Emphasis is on impurities, by-products, contaminants and other artifacts. A separate entry is provided for each artifact. For specific chemicals, this entry provides the common name, mass spectrum, gas chromatographic data, CAS name and registry number, synonyms and a narrative discussion. More than 1100 entries are included. Mass spectral data are indexed in a 6-peak index (molecular ion, base peak, second peak, third peak) and there are also formula, author and subject indexes. An extensive bibliography contains complete literature citations.The book is designed to be used. It will not only allow experienced analysts to profit from the mistakes of others, but it will also be invaluable to other scientists who use analytical instruments in their work.

Liquid Chromatography of Natural Pigments and Synthetic Dyes

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Publisher : Elsevier
ISBN 13 : 0080465765
Total Pages : 603 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Liquid Chromatography of Natural Pigments and Synthetic Dyes by : Tibor Cserháti

Download or read book Liquid Chromatography of Natural Pigments and Synthetic Dyes written by Tibor Cserháti and published by Elsevier. This book was released on 2006-12-07 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt: This latest volume in the series entitled Liquid Chromatography of Natural Pigments and Synthetic Dyes presents an overview of the latest developments in the field while critically evaluating this method of analysis and providing comparisons of the various liquid chromatographic separation techniques that are currently available. Natural pigments and synthetic dyes are extensively used in various fields of everyday life including food production, textile industry, paper production, agricultural practice and research and water science and technology. Besides their capacity for increasing the marketability of products, natural pigments have shown advantageous biological activity as antioxidants and anticancer agents. On the negative side, synthetic pigments have a significant impact on the environment and can cause adverse toxicological side effects. Both pigment classes exhibit considerable structural diversity. As the stability of the pigments against hydrolysis, oxidation and other environmental and technological conditions is markedly different, the exact determination of the pigment composition may help for the prediction of the shelf-life of products and the assessment of the influence of technological steps on the pigment fractions resulting in more consumer friend processing methods. Furthermore, the qualitative determination and identification of the pigments may contribute to the establishment of the provenance of the product. The unique separation capacity of liquid chromatographic (LC) techniques makes it a method of preference for the analysis of pigments in any complicated accompanying matrices.* an overview of the latest developments in the field* a critical evaluation of results from this form of analysis* a comparison of the various LC (liquid chromatographic) separation techniques* future trends in the LC analysis of pigments

Unified Chromatography

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Publisher :
ISBN 13 :
Total Pages : 272 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Unified Chromatography by : J. F. Parcher

Download or read book Unified Chromatography written by J. F. Parcher and published by . This book was released on 2000 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here, authors specializing in different branches of chromatography--including gas chromatography, supercritical fluid chromatography, and high-pressure liquid chromatography--describe their fields while drawing out connections with other branches.

Introduction to Modern Liquid Chromatography

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Publisher : John Wiley & Sons
ISBN 13 : 1118210395
Total Pages : 831 pages
Book Rating : 4.1/5 (182 download)

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Book Synopsis Introduction to Modern Liquid Chromatography by : Lloyd R. Snyder

Download or read book Introduction to Modern Liquid Chromatography written by Lloyd R. Snyder and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 831 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.