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In Vitro And In Vivo Analyses Of Alternative Splicing Of 3 End Transcripts Of The Drosophila Melanogaster Muscle Myosin Heavy Chain Gene
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Book Synopsis In-vitro and In-vivo Analyses of Alternative Splicing of 3' End Transcripts of the Drosophila Melanogaster Muscle Myosin Heavy Chain Gene by : Dorothy Dianne Hodges
Download or read book In-vitro and In-vivo Analyses of Alternative Splicing of 3' End Transcripts of the Drosophila Melanogaster Muscle Myosin Heavy Chain Gene written by Dorothy Dianne Hodges and published by . This book was released on 1992 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt: Up to 480 isoforms of Drosophila muscle myosin heavy chain (MHC) can be generated by the process of alternative splicing. In order to better understand the regulation of MHC expression, we have analyzed the alternative splicing of MHC 3' end transcripts in vitro and in vivo. In Chapter 1 we describe the development and use of a Drosophila in-vitro splicing system to study the alternative splicing of penultimate exon 18. We demonstrate that pre-mRNA is spliced to exclude exon 18, as occurs in embryonic and larval muscle in vivo. However, when the 5' and 3' splice sites of exon 18 are modified to improve their binding to constitutive splicing factors, exon 18 is efficiently spliced to both flanking exons, as occurs in adult muscles in vivo. In Chapter 2 we express similarly modified transcripts in vivo using P element mediated germ line transformation. Mini-gene transcripts in which both splice sites of exon 18 are improved are now spliced to include exon 18 in larvae, as well as in adults. This is a complete splicing switch; all mRNAs typical of the normal larval splicing pattern have been eliminated. We also demonstrate that the correct 3' splice site of exon 18 is not utilized by the larval splicing machinery, even when the competing downstream 3' splice site is eliminated. Analysis of MHC [Delta] Int 17 mini-gene transcript splicing determined that intron 17 sequences are needed for intron 18 removal in larvae and adults. We also present results of cloning and sequencing the distantly related D. virilis MHC gene. Large stretches of non-coding sequences within exon 18 and a pyrimidine rich element in intron 17 are conserved between the D. virilis and D. melanogaster MHC genes. Mini-gene transcripts lacking most of the conserved exon 18 sequences were spliced in the correct stage-specific manner in vivo. However, analysis of splicing of mini-gene transcripts lacking the polypyrimidine sequence confirmed that it is essential for correct inclusion of exon 18 in adult mRNA, and suggests that binding of adult-specific, transacting factors to this element may mediate recognition and utilization of the weak 3' splice site of exon 18.
Download or read book Drosophila Information Service written by and published by . This book was released on 1934 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Journal of Cell Biology written by and published by . This book was released on 1991 with total page 904 pages. Available in PDF, EPUB and Kindle. Book excerpt: No. 2, pt. 2 of November issue each year from v. 19-47; 1963-70 and v. 55- 1972- contain the Abstracts of papers presented at the annual meeting of the American Society for Cell Biology, 3d-10th; 1963-70 and 12th- 1972- .
Book Synopsis Abstracts, 20th Annual Meetings, January 24-February 2, 1991 by :
Download or read book Abstracts, 20th Annual Meetings, January 24-February 2, 1991 written by and published by . This book was released on 1991 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1992 with total page 796 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2005 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1985 with total page 1094 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Entomology Abstracts written by and published by . This book was released on 1992 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monthly, with annual author and subject indexes. Abstracts from about 2750 primary journals dealing with the subject of insects. Arranged in classified order. Entries include titles given or translated into English, authors, addresses offirst authors, and abstracts; all insects cited in the abstracts are identified by scientific family names. Each monthly issue has Index to classes and orders, Author index.
Book Synopsis Abstracts, 23rd Annual Meetings , February 26-April 17, 1994 by :
Download or read book Abstracts, 23rd Annual Meetings , February 26-April 17, 1994 written by and published by . This book was released on 1994 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biochemistry of Smooth Muscle Contraction by : Michael Barany
Download or read book Biochemistry of Smooth Muscle Contraction written by Michael Barany and published by Elsevier. This book was released on 1996-01-04 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: This valuable resource provides a systematic account of the biochemistry of smooth muscle contraction. As a comprehensive guide to this rapidly growing area of research, it covers the structure and characteristic properties of contractile and regulatory proteins, with special emphasis on their predicted function in the live muscle. Also included in this book are intermediate filament proteins, and desmin and vimentin, whose function in smooth muscle is unknown; and several enzymes involved in the phosphorylation-dephosphorylation of contractile and other proteins.
Download or read book Nucleic Acids Research written by and published by . This book was released on 2001 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetics Abstracts written by and published by . This book was released on 1998 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Myofibrillogenesis by : Dipak K. Dube
Download or read book Myofibrillogenesis written by Dipak K. Dube and published by Springer Science & Business Media. This book was released on 2001-10-19 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Myofibrillogenesis has been studied extensively over the last 100 years. Until recently, we have not had a comprehensive understanding of this fundamental process. The emergence of new technologies in molecular and cellular biology, combined with classical embryology, have started to unravel some of the complexities of myofibril assembly in striated muscles. In striated muscles, the contractile proteins are arranged in a highly ordered three dimensional lattice known as the sarcomere. The assembly of a myofibril involves the precise ordering of several proteins into a linear array of sarcomeres. Multiple isoforms in many of these proteins further complicate the process, making it difficult to define the precise role of each component. This volume has been compiled as a comprehensive reference on myofibrillogenesis. In addition, the book includes reviews on myofibrillar disarray under various pathological conditions, such as familial hypertrophic cardiomyopathy (FHC), and incorporates a section on the conduction system in the heart. Much of the information in this volume has not been described elsewhere. Presented in a manner to be of value to students and teachers alike, "Myofibrillogenesis" will be an invaluable reference source for all in the fields of muscle biology and heart development.
Book Synopsis Biological & Agricultural Index by :
Download or read book Biological & Agricultural Index written by and published by . This book was released on 1991 with total page 2570 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Agrindex written by and published by . This book was released on 1992 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2710 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2000 with total page 700 pages. Available in PDF, EPUB and Kindle. Book excerpt:
