In Situ Hybridization In Neurobiology

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In Situ Hybridization in Neurobiology

Author : James H. Eberwine
Publisher :
ISBN 13 : 9780195075076
Total Pages : 230 pages
Book Rating : 4.0/5 (75 download)

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Book Synopsis In Situ Hybridization in Neurobiology by : James H. Eberwine

Download or read book In Situ Hybridization in Neurobiology written by James H. Eberwine and published by . This book was released on 1994 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.

In Situ Hybridization Protocols for the Brain

Author : W. Wisden
Publisher : Gulf Professional Publishing
ISBN 13 : 9780123668479
Total Pages : 312 pages
Book Rating : 4.6/5 (684 download)

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Book Synopsis In Situ Hybridization Protocols for the Brain by : W. Wisden

Download or read book In Situ Hybridization Protocols for the Brain written by W. Wisden and published by Gulf Professional Publishing. This book was released on 2002-08-05 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. A well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research

In Situ Hybridization

Author : Karen L. Valentino
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 280 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis In Situ Hybridization by : Karen L. Valentino

Download or read book In Situ Hybridization written by Karen L. Valentino and published by Oxford University Press, USA. This book was released on 1987 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: A technique for reading the messenger RNAs by which cells express their distinctive populations of structural and functional proteins, in situ hybridization is now reliable and practical for use in neurobiological research.

In Situ Hybridization in Brain

Author : George Uhl
Publisher : Springer Science & Business Media
ISBN 13 : 1461594863
Total Pages : 298 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis In Situ Hybridization in Brain by : George Uhl

Download or read book In Situ Hybridization in Brain written by George Uhl and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. Workers with backgrounds in peptide neuroanatomy, neuropharmacology, molecular biology, neurovirology, neuropathology, and neurophysiology have joined together in this volume to discuss their initial experiences in applying ill situ hybridization techniques to the study of the brain. The work, although still in an early phase of development, is worthy of initial summary and dissemination. In the area of neuropeptide gene expression alone, investigators represented here describe studies of vasopressin, opiate peptides, oxytocin, vasoactive intestinal peptide, cholecystokinin, and somatostatin. Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain. The authors provide an important series of technical perspectives, and describe specific experimental protocols. This volume should be of interest to individuals seeking an introduction to these methods, as well to those desiring an up to date precis of work in this burgeoning area. Dr. Uhl, with the sponsorship of the Howard Hughes Medical Institute, has done a superb job of assembling the leaders in this area, and in organizing the presen ta tion of their perspecti ves herein. Joseph B. Martin, M.D., Ph.D.

In Situ Hybridization Techniques for the Brain

Author : Z. Henderson
Publisher :
ISBN 13 :
Total Pages : 192 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis In Situ Hybridization Techniques for the Brain by : Z. Henderson

Download or read book In Situ Hybridization Techniques for the Brain written by Z. Henderson and published by . This book was released on 1996-12-11 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.

In Situ Hybridization Methods

Author : Giselbert Hauptmann
Publisher : Humana
ISBN 13 : 9781493944637
Total Pages : 0 pages
Book Rating : 4.9/5 (446 download)

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Book Synopsis In Situ Hybridization Methods by : Giselbert Hauptmann

Download or read book In Situ Hybridization Methods written by Giselbert Hauptmann and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

In Situ Hybridization

Author : Ashley T. Haase
Publisher : Springer Science & Business Media
ISBN 13 : 3642744257
Total Pages : 92 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis In Situ Hybridization by : Ashley T. Haase

Download or read book In Situ Hybridization written by Ashley T. Haase and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading researchers present contemporary treatment of in situ hybridization applied to current issues in animal virus pathogenesis. The most recent methods are given for locating viral genes in whole animal section and for defining the number and type of cells surrounded by viruses. The genetic programs played out in these cells and the newer methods of hybridization at the electron microscopic level provide valuable insight into the complexities of virus-host interaction.

Molecular Imaging in Neuroscience

Author : Najam Arfeen Sharif
Publisher :
ISBN 13 : 9781383048513
Total Pages : 0 pages
Book Rating : 4.0/5 (485 download)

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Book Synopsis Molecular Imaging in Neuroscience by : Najam Arfeen Sharif

Download or read book Molecular Imaging in Neuroscience written by Najam Arfeen Sharif and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A practical book on advances in modern imaging techniques, this guide to exploiting these techniques presents detailed protocols for imaging neurotransmitter and receptor mRNAs, using radioactive and non-radioactive in situ hybridization technology.

Special Issue, in Situ Hybridization

Author : Walter Scott Young
Publisher :
ISBN 13 :
Total Pages : 174 pages
Book Rating : 4.:/5 (222 download)

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Book Synopsis Special Issue, in Situ Hybridization by : Walter Scott Young

Download or read book Special Issue, in Situ Hybridization written by Walter Scott Young and published by . This book was released on 1990 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:

In Situ Hybridization

Author : D. G. Wilkinson
Publisher : Oxford University Press, USA
ISBN 13 : 9780199636594
Total Pages : 224 pages
Book Rating : 4.6/5 (365 download)

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Book Synopsis In Situ Hybridization by : D. G. Wilkinson

Download or read book In Situ Hybridization written by D. G. Wilkinson and published by Oxford University Press, USA. This book was released on 1998 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function ofgenes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technicaladvances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electronmicroscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situhybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach(OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.

Molecular Neuroanatomy

Author : Fred W. Leeuwen
Publisher : Elsevier Publishing Company
ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Molecular Neuroanatomy by : Fred W. Leeuwen

Download or read book Molecular Neuroanatomy written by Fred W. Leeuwen and published by Elsevier Publishing Company. This book was released on 1988 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: For a thorough study of the dynamics of particular brain compounds it is now possible to use and combine various molecular neuroanatomical methods (e.g. in situ hybridization, receptor localisation and immunocytochemistry) in a quantitative way on whole brain sections maintaining morphological details. Molecular Neuroanatomy deals with the many practical aspects and recent developments in these areas. The theoretical background of many techniques is presented, as well as clear, step-by-step instructions on the preparation and application of all the methods and techniques described in this book. It will be invaluable to all those working in the field of neuroscience. Available in both hardback and paperback, with colour illustrations.

Gene Expression in Neural Tissues

Author : P. Michael Conn
Publisher :
ISBN 13 :
Total Pages : 522 pages
Book Rating : 4.X/5 (2 download)

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Book Synopsis Gene Expression in Neural Tissues by : P. Michael Conn

Download or read book Gene Expression in Neural Tissues written by P. Michael Conn and published by . This book was released on 1992 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of gene expression in neural tissue brings to bear some of the newest and most productive scientific technology to one of the most exciting and fastest growing areas. This volume describes methods for the study of the expression of specific genes frequently found in very low abundance. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * Comprehensive protocols included for * In situ hybridization * Comparison of in situ and immunochemical methods for the study of regulatory molecules * Gene transfer * The study of the regulation of gene expression * The enhancer trap method * Quantifying rare mRNAs * Isolation and use of cell lines * The homology approach for receptor cloning * Trouble Shooting

Brain Development

Author : Simon G. Sprecher
Publisher : Humana Press
ISBN 13 : 9781627036566
Total Pages : 317 pages
Book Rating : 4.0/5 (365 download)

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Book Synopsis Brain Development by : Simon G. Sprecher

Download or read book Brain Development written by Simon G. Sprecher and published by Humana Press. This book was released on 2013-09-19 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.

Molecular Neurobiological Techniques

Author : Alan A. Boulton
Publisher : Springer Science & Business Media
ISBN 13 : 1592596215
Total Pages : 306 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Molecular Neurobiological Techniques by : Alan A. Boulton

Download or read book Molecular Neurobiological Techniques written by Alan A. Boulton and published by Springer Science & Business Media. This book was released on 2008-02-06 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: It goes without saying that the principles and techniques of molecular biology are having and will continue to have a major impact on investigations into nervous system structure and func tion. It is becoming increasingly apparent to neuroscientists in all subdisciplines that a working knowledge of the language, approaches, and techniques of molecular biology is indispensable for their work. For these reasons, the editors have decided to devote this volume of Neuromethods to the techniques of molecular biology and their application to neural systems. There currently exist a number of excellent reference technical manuals that de scribe molecular neurobiological techniques in great detail, and many of these are cited within the chapters included in this volume. It was not the intention of the editors or authors of this volume to duplicate these efforts. Rather, our intention was to present to the neuroscientist who is relatively unfamiliar with these methodologies an understanding of how specific techniques are used to approach major molecular neurobiological problems as well as a set of techniques that work in the laboratories of the individuals writing the chapters. In some cases, there are duplica tions of techniques these have been retained to illustrate the range of variability of the technique and/or the flexibility of the method to study different types of problems. We hope that the chapters will provide the reader with an understanding of the methods and their applicability to neurobiological problems; and, perhaps, suggest new directions for the reader's research efforts. Anthony T.

In Vitro Neurochemical Techniques

Author : Alan A. Boulton
Publisher : Humana Press
ISBN 13 : 9781617370557
Total Pages : 296 pages
Book Rating : 4.3/5 (75 download)

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Book Synopsis In Vitro Neurochemical Techniques by : Alan A. Boulton

Download or read book In Vitro Neurochemical Techniques written by Alan A. Boulton and published by Humana Press. This book was released on 2010-10-28 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Vitro Neurochemical Techniques is one of three volumes that rep- sent an update of the original Volume 1 of Neuromefkods, General Neu- chemical Techniques The expansion to three volumes was necessitated by the rapidly increasing number of techniques being used by neuroscrentists and the general tendency of neuroscience research to become more multidisciplrnary and collaborative in recent years. The two companion volumes are concerned primarily with In Vwo Neurometkods in one case, and with Cell Neurobiology Techniques in the other case. Even with three volumes, the coverage of General Neuvometkods is obviously not exhaustive. We have attempted to cover those techniques that seem particularly popular in multidisciplinary neuroscience research. Though some important techniques may have been omitted, we believe that most of these have been covered in detail in other recent volumes of Neurometkods or will be the subjects of upcommg volumes The editors anticipate that these volumes will prove unusually useful to neuroscientists across a wide range of fields-e.g., neurochemistry, neuropsychopharmacology, neurology, psychiatry, and neuroanatomy. Each chapter has been written by well-known neuroscientists with - tive research programs. Experienced researchers in the specific areas treated will appreciate the present updates on those topics, and neuros- entists expecting their research programs to become more multidisciplinary or to expand into new areas of research should find these volumes of enormous value, particularly those sections offering protocols with u- ful hints about the advantages and potential pitfalls of a particular te- nique.

In Situ Hybridization Studies of Developmental Gene Expression in Postnatal Rat Brain Stem

Author : Camille Christine Michaud
Publisher :
ISBN 13 :
Total Pages : 40 pages
Book Rating : 4.:/5 (57 download)

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Book Synopsis In Situ Hybridization Studies of Developmental Gene Expression in Postnatal Rat Brain Stem by : Camille Christine Michaud

Download or read book In Situ Hybridization Studies of Developmental Gene Expression in Postnatal Rat Brain Stem written by Camille Christine Michaud and published by . This book was released on 2004 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt:

In Situ Hybridization Studies of Intrinsic and Extrinsic Influences on Gene Expression in Purkinje Neurons in the Developing Cerebellum of Mutant Mice

Download In Situ Hybridization Studies of Intrinsic and Extrinsic Influences on Gene Expression in Purkinje Neurons in the Developing Cerebellum of Mutant Mice PDF Online Free

Author : Carol Wilde Wuenschell
Publisher :
ISBN 13 :
Total Pages : 638 pages
Book Rating : 4.:/5 (311 download)

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Book Synopsis In Situ Hybridization Studies of Intrinsic and Extrinsic Influences on Gene Expression in Purkinje Neurons in the Developing Cerebellum of Mutant Mice by : Carol Wilde Wuenschell

Download or read book In Situ Hybridization Studies of Intrinsic and Extrinsic Influences on Gene Expression in Purkinje Neurons in the Developing Cerebellum of Mutant Mice written by Carol Wilde Wuenschell and published by . This book was released on 1988 with total page 638 pages. Available in PDF, EPUB and Kindle. Book excerpt: