Immunogold-Silver Staining

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Publisher : CRC Press
ISBN 13 : 1040290531
Total Pages : 337 pages
Book Rating : 4.0/5 (42 download)

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Book Synopsis Immunogold-Silver Staining by : M. A. Hayat

Download or read book Immunogold-Silver Staining written by M. A. Hayat and published by CRC Press. This book was released on 2024-11-01 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

Gold and Silver Staining

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Author :
Publisher : CRC Press
ISBN 13 : 9780849313929
Total Pages : 264 pages
Book Rating : 4.3/5 (139 download)

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Book Synopsis Gold and Silver Staining by : Gerhard W. Hacker

Download or read book Gold and Silver Staining written by Gerhard W. Hacker and published by CRC Press. This book was released on 2002-04-17 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know about the various staining methods, their useful applications, and the advantages and drawbacks of each process. Gold and Silver Staining: Techniques in Molecular Morphology provides a timely description of approaches, methods, protocols, and applications. The contributors cover the latest developments and a wide range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. Some of the authors are "living legends" in the field and bring their expertise and experience to this fine collaboration. Written in one consistent style, each chapter includes a concise, but comprehensive introduction, step-by-step protocols with technical hints, and a discussion of results and critical steps. What differentiates this book from all others like it, is the status of the editors, who have worked on this technique from its inception and have produced innumerable publications on the topic. The other distinguishing feature is that all of the contributors are amongst the absolute foremost leaders from the United States and Europe. Gold and Silver Staining: Techniques in Molecular Morphology presents a complete overview and detailed descriptions of this technique that allows the visualization of molecules that have never been localized before and with hitherto unknown sensitivity. Not only does this book provide an excellent review of this field, but it also serves as a lab manual for those who want to carry out this technique in their laboratory and clinical work. Armed with this information, advances in this powerful field of research will ensue.

Gold and Silver Staining

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Author :
Publisher : CRC Press
ISBN 13 : 1420040235
Total Pages : 265 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Gold and Silver Staining by : Gerhard W. Hacker

Download or read book Gold and Silver Staining written by Gerhard W. Hacker and published by CRC Press. This book was released on 2002-04-17 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a

Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

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Author :
Publisher : CRC Press
ISBN 13 : 9780849367595
Total Pages : 476 pages
Book Rating : 4.3/5 (675 download)

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Book Synopsis Immuno-Gold Electron Microscopy in Virus Diagnosis and Research by : Alexander D. Hyatt

Download or read book Immuno-Gold Electron Microscopy in Virus Diagnosis and Research written by Alexander D. Hyatt and published by CRC Press. This book was released on 1992-11-10 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Colloidal Gold

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Author :
Publisher : Elsevier
ISBN 13 : 0323139027
Total Pages : 517 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Colloidal Gold by :

Download or read book Colloidal Gold written by and published by Elsevier. This book was released on 2012-12-02 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. - Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books - Problems and limitations of techniques are discussed - Guides users to avoid problems and choose the correct procedures for specific applications - Contributors are eminent authorities in their fields

Analytical Morphology

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 9780817639570
Total Pages : 308 pages
Book Rating : 4.6/5 (395 download)

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Book Synopsis Analytical Morphology by : Jiang Gu

Download or read book Analytical Morphology written by Jiang Gu and published by Springer Science & Business Media. This book was released on 1997-03-20 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical Morphology is a new discipline dealing with the analysis of cell and tissue components using analytical maneuvers that go beyond those of conventional morphology and histopathology. It utilizes new advances in other disciplines, such as immunology, molecular biology, laser technology, microwave technology and computer science, to highlight cellular components such as peptides, proteins, or nucleotide sequences through chemical, physical, immunological, or molecular biological reactions. These methods include immunocytochemistry, in situ hybridization, in situ PCR, antigen retrieval, image analysis, and the like. The book is intended for morphological scientists, pathologists, advanced students, and technicians who are interested in keeping abreast of this rapidly emerging discipline.

Leong's Manual of Diagnostic Antibodies for Immunohistology

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Author :
Publisher : Cambridge University Press
ISBN 13 : 1316592065
Total Pages : 525 pages
Book Rating : 4.3/5 (165 download)

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Book Synopsis Leong's Manual of Diagnostic Antibodies for Immunohistology by : Runjan Chetty

Download or read book Leong's Manual of Diagnostic Antibodies for Immunohistology written by Runjan Chetty and published by Cambridge University Press. This book was released on 2016-11-28 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a unique A-Z guide to antibodies for immunohistology, this is an indispensable source for pathologists to ensure the correct application of immunohistochemistry in daily practice. Each entry includes commercial sources, clones, descriptions of stained proteins/epitopes, the full staining spectrum of normal and tumor tissues, staining pattern and cellular localization, the range of conditions of immunoreactivity, and pitfalls of the antibody's immunoprofile, giving pathologists a truly thorough quick-reference guide to sources, preparation and applications of specific antibodies. Appendices provide useful quick-reference tables of antibody panels for differential diagnoses, as well as summaries of diagnostic applications. Expanded from previous editions with over forty new entries, this handbook for diagnostic, therapeutic, prognostic and research applications of antibodies is an essential desktop book for practicing pathologists as well as researchers, residents and trainees.

Neuroanatomical Tract-Tracing Methods 2

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Publisher : Springer Science & Business Media
ISBN 13 : 1475720556
Total Pages : 429 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Neuroanatomical Tract-Tracing Methods 2 by : Laszlo Zaborszky

Download or read book Neuroanatomical Tract-Tracing Methods 2 written by Laszlo Zaborszky and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition presents readers with the latest information on neuroscience. This book explores the advances in molecular techniques, genomics and proteomics and the progress in fluorescence.

The Protein Protocols Handbook

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Publisher : Springer Science & Business Media
ISBN 13 : 0896039404
Total Pages : 1446 pages
Book Rating : 4.8/5 (96 download)

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Book Synopsis The Protein Protocols Handbook by : John M. Walker

Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2002 with total page 1446 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.

Biological Field Emission Scanning Electron Microscopy, 2 Volume Set

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 1118654064
Total Pages : 741 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by : Roland A. Fleck

Download or read book Biological Field Emission Scanning Electron Microscopy, 2 Volume Set written by Roland A. Fleck and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 741 pages. Available in PDF, EPUB and Kindle. Book excerpt: The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Analytical Morphology

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1461241669
Total Pages : 298 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Analytical Morphology by : Jiang Gu

Download or read book Analytical Morphology written by Jiang Gu and published by Springer Science & Business Media. This book was released on 2013-12-11 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Progress in Surgical Pathology

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Publisher : Springer Science & Business Media
ISBN 13 : 3662128144
Total Pages : 406 pages
Book Rating : 4.6/5 (621 download)

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Book Synopsis Progress in Surgical Pathology by : Cecilia M. Fenoglio-Preiser

Download or read book Progress in Surgical Pathology written by Cecilia M. Fenoglio-Preiser and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: ****VERKAUFSKATEGORIE*** 0 e.

Molecular Morphology in Human Tissues

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Publisher : CRC Press
ISBN 13 : 0203503481
Total Pages : 328 pages
Book Rating : 4.2/5 (35 download)

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Book Synopsis Molecular Morphology in Human Tissues by : Gerhard W. Hacker

Download or read book Molecular Morphology in Human Tissues written by Gerhard W. Hacker and published by CRC Press. This book was released on 2004-12-29 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Morphology in Human Tissues: Techniques and Applications presents the most advanced molecular morphological techniques to date. This integrated approach to molecular morphology provides powerful analytical and diagnostic tools at the genome level, making the diagnosis and management of cancer, viral infections, and other diseases more pre

Immunoenzymatic Techniques

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Publisher : Elsevier Science & Technology
ISBN 13 :
Total Pages : 428 pages
Book Rating : 4.:/5 (44 download)

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Book Synopsis Immunoenzymatic Techniques by : S. Avrameas

Download or read book Immunoenzymatic Techniques written by S. Avrameas and published by Elsevier Science & Technology. This book was released on 1983 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aandacht voor de enzymatische-immunocytochemie met de nadruk op intracellulaire penetratie van gelabelde antilichamen en de toepassing van "double staining"-methodes of monoclonale antilichamen in normale en in diverse experimentele of pathologische omstandigheden; kwantitatieve aspecten van immuno-enzymatische technieken, zoals nieuwe technische vindingen en toepassingen op verschillende gebieden, bijvoorbeeld biologie, humane en veterinaire geneeskunde, fytopathologie en experimentele ziekten

The Protein Protocols Handbook

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Author :
Publisher : Springer
ISBN 13 : 1592591698
Total Pages : 1099 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis The Protein Protocols Handbook by : John M. Walker

Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1099 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.

Immunocytochemistry

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Publisher : CRC Press
ISBN 13 : 1000098699
Total Pages : 289 pages
Book Rating : 4.0/5 ( download)

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Book Synopsis Immunocytochemistry by : Lars-Inge Larsson

Download or read book Immunocytochemistry written by Lars-Inge Larsson and published by CRC Press. This book was released on 2020-11-25 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: A complete and balanced overview of all aspects of immunocytochemistry is presented providing a clear understanding of their impact on experiment. All available techniques and many diagnostic and research applications are included, as well as practical step-by-step instructions for carrying out recommended methods. Intended for the novice as well as the experienced researchers.

Modern Methods in Analytical Morphology

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Publisher : Springer Science & Business Media
ISBN 13 : 1461525322
Total Pages : 441 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Modern Methods in Analytical Morphology by : J. Gu

Download or read book Modern Methods in Analytical Morphology written by J. Gu and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: While advances in modem medicine largely parallel our understanding of morphology, discoveries in morphology are propelled by developments of new tools and means to visualize and measure tissue elements. The invention of dissecting, light, fluorescence and electron microscopes together with advances in labeling and staining techniques are among the stepping stones of morphological progress. Today, we are in an exciting new era when classical morphology is being combined with developments from other disciplines. The combination of morphology and immunology resulted in immunocytochemistry; morphology and molecular biology led to in situ hybridization and in situ PCR. Adding computer science to morphology gave birth to image analysis. Combining laser technology and the microsope evolved into confocal microscope. For more than a decade, modem morphology has continued to develop by merging with other disciplines at a rate that is still gathering momentum, providing exciting and dynamic new frontiers for other biological fields. "Modem Methods in Analytical Morphology," based largely on the "First International Workshop on Modem Methods in Analytical Histochemistry, "is an updated review of the current trends in the field. It covers an extensive array of new technical developments in major disciplines of modem morphology. The authors are not only leaders in their fields but also have extensive "hands on" experience with "bench work. " Their chapters are written in a comprehensive manner including discussion of both theoretical considerations and practical applications to give the readers a broad view of the topics covered.