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Immune And Receptor Assays In Theory And Practice
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Book Synopsis Immune and Receptor Assays in Theory and Practice by : Patrick Englebienne
Download or read book Immune and Receptor Assays in Theory and Practice written by Patrick Englebienne and published by CRC Press. This book was released on 1999-12-28 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past ten years, employment of immuno- and receptor- assays has grown dramatically. Now used in the pharmaceutical industry for automated screening programs, in the agro-food industry for on-line processing control and food adulteration detection, and in clinical laboratories, they are fully integrated analytical tools. However, the literature often covers only one type of assay or just one of the many systems available. Immune and Receptor Assays in Theory and Practice gathers and organizes the available information to help you establish the best assay for your application. This composite presents the fundamentals of both techniques and introduces practical examples of equation use, antibody and receptor purification, antigen labeling, immunization, and establishment of antibodies for long-term storage. It contrasts the many different assay designs and addresses market trends as the context for developing immuno-assay goals. In addition, this volume summarizes the biochemical and physical properties involved in antibody- and receptor-ligand interactions and reagent manufacture. This is the first, single-volume synthesis of both immuno- and receptor-assays. With theoretical background and practical examples, Immune and Receptor Assays in Theory and Practice allows you to base your experiment on proven techniques, components, and applications for the most reliable results.
Book Synopsis Immunochemistry of Solid-Phase Immunoassay by : John E. Butler
Download or read book Immunochemistry of Solid-Phase Immunoassay written by John E. Butler and published by CRC Press. This book was released on 1991-07-23 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.
Book Synopsis Chronic Fatigue Syndrome by : Patrick Englebienne
Download or read book Chronic Fatigue Syndrome written by Patrick Englebienne and published by CRC Press. This book was released on 2002-02-27 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chronic Fatigue Syndrome (CFS) is a complex, debilitating disorder, yet few current scientific biomedical books are available on the subject. The nonspecific symptoms, lack of diagnostic tests, and uncertainty as to the cause or causes of CFS make the disease that much more baffling. Chronic Fatigue Syndrome: A Biological Approach represents a monu
Book Synopsis Development and Application of Biomarkers by : Roger L. Lundblad
Download or read book Development and Application of Biomarkers written by Roger L. Lundblad and published by CRC Press. This book was released on 2016-04-19 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: First introduced to biomedical research in 1980, the term biomarker has taken on a life of its own in recent years and has come to mean a number of things. In biomedical science, biomarker has evolved to most commonly mean a characteristic that can be used either as a diagnostic or a prognostic, but most significantly as a screening indicator for p
Download or read book Phage Display written by Tim Clackson and published by Oxford University Press, USA. This book was released on 2004 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures continue to evolve. Phage Display - A Practical Approach is an up-to-date, comprehensive and integrated experimental guide to the technique, useful for novice and expert alike. The book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. An introductory chapter provides an overview of phage biology and phage display, including guidelines for planning a successful phage display experiment. Individual chapters provide protocols for constructing libraries using oligonucleotide-directed mutagenesis or DNA recombination, performing binding selections, and analyzing the binding activities of selected phage clones. Separate chapters then cover common applications, including selection of ligands from peptide libraries, generation of phage antibody libraries and isolation and optimization of antibodies, selection of DNA binding proteins, and expression cloning using cDNA display. Further chapters describe alternative selection strategies, such as selection using immune sera, selection based on enzymatic activity or protein stability, and selection in vivo. Protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples given to customize the procedures to their own protein or selection system of interest. Written by experts in the field, Phage Display - A Practical Approach provides a comprehensive guide to the design and execution of phage display projects, for all those using the technique in basic research and drug discovery.
Book Synopsis An Atlas of Reproductive Physiology in Men by : B. Hafez
Download or read book An Atlas of Reproductive Physiology in Men written by B. Hafez and published by CRC Press. This book was released on 2024-11-01 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meeting the needs of professionals in this growing specialty, Atlas of Reproductive Physiology in Men summarizes some of the latest research in the field. Packed with tables, color illustrations, and photographs, the book covers laboratory techniques and morphological, anatomical, biochemical, immunological, hereditary, and microbiological parameters and their clinical applications. A comprehensive and well-referenced resource, the atlas bridges the gap between basic and clinical science.
Book Synopsis Biochemistry and Molecular Biology Compendium by : Roger L. Lundblad
Download or read book Biochemistry and Molecular Biology Compendium written by Roger L. Lundblad and published by CRC Press. This book was released on 2019-11-11 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an accessible resource offering practical information not found in more database-oriented resources. The first chapter lists acronyms with definitions, and a glossary of terms and subjects used in biochemistry, molecular biology, biotechnology, proteomics, genomics, and systems biology. There follows chapters on chemicals employed in biochemistry and molecular biology, complete with properties and structure drawings. Researchers will find this book to be a valuable tool that will save them time, as well as provide essential links to the roots of their science. Key selling features: Contains an extensive list of commonly used acronyms with definitions Offers a highly readable glossary for systems and techniques Provides comprehensive information for the validation of biotechnology assays and manufacturing processes Includes a list of Log P values, water solubility, and molecular weight for selected chemicals Gives a detailed listing of protease inhibitors and cocktails, as well as a list of buffers
Book Synopsis Application of Solution Protein Chemistry to Biotechnology by : Roger L. Lundblad
Download or read book Application of Solution Protein Chemistry to Biotechnology written by Roger L. Lundblad and published by CRC Press. This book was released on 2009-05-12 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the versatility of the author's science and the depth of his experience, Application of Solution Protein Chemistry to Biotechnology explores key contributions that protein scientists can make in the development of products that are both important and commercially viable, and provides them with tools and information required for successfu
Book Synopsis Environmental Health Perspectives by :
Download or read book Environmental Health Perspectives written by and published by . This book was released on 2000 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Recognition of Carbohydrates in Biological Systems, Part A: General Procedures by : Y. C. Lee
Download or read book Recognition of Carbohydrates in Biological Systems, Part A: General Procedures written by Y. C. Lee and published by Elsevier. This book was released on 2003-09-15 with total page 667 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume, Recognition of Carbohydrates in Biological Systems, Part A: General Procedures, and its companion, Volume 363, present state-of-the-art methodologies, as well as the most recent biological observations in this area. Covers the isolation/synthesis of substances used in studying interactions involving carbohydrates Discussed the methodology for measuring such interactions Biological roles for such interactions are also covered
Book Synopsis Frontiers in Drug Design and Discovery by : Atta-ur- Rahman
Download or read book Frontiers in Drug Design and Discovery written by Atta-ur- Rahman and published by Bentham Science Publishers. This book was released on 2010-12-13 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Frontiers in Drug Design and Discovery" is an Ebook series devoted to publishing the latest and the most important advances in drug design and discovery. Eminent scientists write contributions on all areas of rational drug design and drug discovery inclu
Book Synopsis Biotechnology of Plasma Proteins by : Roger L. Lundblad
Download or read book Biotechnology of Plasma Proteins written by Roger L. Lundblad and published by CRC Press. This book was released on 2012-08-08 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fractionation of human blood plasma can be considered to be a mature industry, with the basic technology, alcohol fractionation, dating back at least to the 1940s. Many of the products described in the current work have been approved biologics since the 1950s. The information gathered from the development of plasma proteins has proved vital to the development of recombinant therapeutic proteins. Discussing the role of plasma proteins in current biotechnology, Biotechnology of Plasma Proteins describes the protein composition of human plasma, the fractionation of plasma to obtain therapeutic proteins, and the analysis of these products. It delineates the path from plasma products to recombinant products, and highlights products from albumin, intravenous immunoglobins, and coagulation. It offers a comprehensive review of current techniques for the analysis of proteins including electrophoresis, chromatography, spectrophotometry, mass spectrometry, and updates not published since 1975. Key Topics Protein Composition of Plasma Proteomic methods for plasma protein analysis Plasma protein biomarkers Validation of biomarkers Assays for plasma biomarkers Methods for the Analysis of Protein Products Assay development and validation Electrophoresis Chromatography Immunoassay Mass spectrometry Raman spectroscopy Plasma Fractionation: Historical and Modern Methods Development of Cohn alcohol fractionation Industrial methods Development of chromatographic methods Plasma Protein Products of Therapeutic Value Albumin Intravenous immunoglobulin Coagulation products Growth factors Wound management
Book Synopsis Contemporary Practice in Clinical Chemistry by : William Clarke
Download or read book Contemporary Practice in Clinical Chemistry written by William Clarke and published by Academic Press. This book was released on 2020-06-11 with total page 1070 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contemporary Practice in Clinical Chemistry, Fourth Edition, provides a clear and concise overview of important topics in the field. This new edition is useful for students, residents and fellows in clinical chemistry and pathology, presenting an introduction and overview of the field to assist readers as they in review and prepare for board certification examinations. For new medical technologists, the book provides context for understanding the clinical utility of tests that they perform or use in other areas in the clinical laboratory. For experienced laboratorians, this revision continues to provide an opportunity for exposure to more recent trends and developments in clinical chemistry. - Includes enhanced illustration and new and revised color figures - Provides improved self-assessment questions and end-of-chapter assessment questions
Book Synopsis Clinical Immunology and Serology by : Linda E. Miller
Download or read book Clinical Immunology and Serology written by Linda E. Miller and published by F.A. Davis. This book was released on 2021-01-28 with total page 1493 pages. Available in PDF, EPUB and Kindle. Book excerpt: The perfect balance of theory and practice! Here’s the practical introduction you need to understand the essential theoretical principles of clinical immunology and the serological and molecular techniques commonly used in the laboratory. You’ll begin with an introduction to the immune system; then explore basic immunologic procedures; examine immune disorders; and study the serological and molecular diagnosis of infectious disease. An easy-to-read, student-friendly approach emphasizes the direct application of theory to clinical laboratory practice. Each chapter is a complete learning module with learning outcomes, chapter outlines, theoretical principles, illustrations, and definitions of relevant terminology. Review questions and case studies help you assess your mastery of the material. A glossary at the end of the book puts must-know information at your fingertips. An access code inside new printed texts unlocks Lab Exercises and Branching Case Studies online at FADavis.com that offer more opportunities to apply theory to clinical laboratory practice.
Book Synopsis Essential Immunology by : Ivan Maurice Roitt
Download or read book Essential Immunology written by Ivan Maurice Roitt and published by . This book was released on 1971 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Proteolysis in the Interstitial Space by : Salvatore V. Pizzo
Download or read book Proteolysis in the Interstitial Space written by Salvatore V. Pizzo and published by CRC Press. This book was released on 2016-08-19 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most clinical laboratory tests utilize interstitial and extravascular such as blood, urine, cerebral spinal fluid (CSF), and saliva. For example, CSF is monitored in the context of cancer for both diagnostic and therapeutic reasons. And yet, our understanding of the makeup of interstitial fluids, their relationships to disease, as well as their commercial importance in therapeutics and diagnostics remains rudimentary. Although sometimes perceived as static, interstitial and extravascular fluids are surprisingly dynamic. More than half of serum albumin is in the extravascular space. These fluids move rapidly between the intravascular and extravascular spaces - one entire plasma volume is exchanged very nine hours. In the first half of the book, the authors cover fundamental concepts of interstitial fluids, including their composition and function. They then further review the mechanisms by which interstitial fluids are regulated, characterizing the importance of hyaluronan – a major constituent of interstitial spaces and an a component of synovial fluid; and, outlining the regulation of proteolysis in the interstitial space. In the second half of the book, the authors focus on the coagulation system. This system has been studied extensively in the context of vascular spaces. But many of its components exist in the interstitial spaces. Chapters are devoted to the fibrinolytic system, kallikrein, matrix metalloproteinases, coagulation factors, and protease inhibitors – all are interstitial. By covering a unique array of topics with broad application to biomedical scientists, this book expands our understanding of the importance of interstitial spaces and the fluids that move through and reside in this extravascular environment.
Book Synopsis Evaluating Endocrine Disruption in Receiving Waters by : P. V. Cline
Download or read book Evaluating Endocrine Disruption in Receiving Waters written by P. V. Cline and published by IWA Publishing. This book was released on 2003-01-01 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, concerns have been raised that low concentrations of chemicals may alter the normal functions of the endocrine system, resulting in potentially significant adverse effects on growth, reproduction, and/or development. For domestic wastewater discharges to surface water bodies, estrogenic activity of effluents has been suggested by chemical analysis, biomarkers, and /or in vitro assays. Chemical Measurements. Using sensitive analytical techniques, natural or synthetic hormones and chemicals that mimic estrogen, have been detected in some domestic wastewater effluents and associated watersheds at low concentrations. Biomarkers. Several studies have demonstrated physiological changes in fish that may be attributed to exposure to estrogenic compounds. These include the presence of vitellogenin protein in male fish and/or intersex conditions. In vitro assays. The Yeast Estrogen Screen (YES) has been used to characterize estrogenicity of effluents, influents, biosolids and surface waters. This two-year WERF project explored approaches to evaluate the potential for biomarker formation as a result of effluent exposures, and the subsequent relevance of the emerging assays and physiological measurements on potential adverse impacts to individuals or populations of fish in the receiving streams. The endocrine system is complex, and many factors can influence the physiological measurements, including methods, sex, age, reproductive status, seasonal and circadian rhythms, diet, temperature, etc. and produce transient changes in physiology but no significant effect on the individual.
