Imaging Membrane Proteins Using Atomic Force Microscopy Techniques

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Imaging Membrane Proteins Using Atomic Force Microscopy Techniques

Author : Joan M. Lau
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (57 download)

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Book Synopsis Imaging Membrane Proteins Using Atomic Force Microscopy Techniques by : Joan M. Lau

Download or read book Imaging Membrane Proteins Using Atomic Force Microscopy Techniques written by Joan M. Lau and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Thousands of different membrane proteins exist in the human body and reside in the membranes of cells. Structural information regarding these proteins can provide insight into the function of these proteins. Acquiring membrane protein structural information is difficult, especially when preparation techniques that are traditionally used for soluble proteins such as crystallizing and isolating proteins are not easily adapted for membrane proteins. The atomic force microscope (AFM) provides an alternative for imaging membrane proteins. The preparation required does not require crystallizing or isolating proteins, and can imagine membrane proteins in their native environment. Techniques are explored that can utilize the AFM for imaging proteins, including examining isolated cell membrane patches and membrane surfaces. One technique utilizes plasma membrane from Xenopus laevis oocytes as a model system to study membrane. A novel technique is developed to isolate oocyte membranes by bursting the oocyte and depositing its membrane on a flat mica substrate. The flat surface membrane preparation allows high-resolution AFM images to be obtained, revealing a novel structure of densely packed particles. These particles exhibit a regular, repeating pattern of a lattice-like array with orderly packing, and are thus termed 3lattice-like array particles4 (LAPs). The LAPs are orderly yet imperfectly packed, are located in depressed pools, occur with a low frequency on the oocyte membrane surface, and have not previously been seen using other isolation and imaging methods. Histogram analysis of the center-to-center distance between LAPs suggest their size to be about 44 nm in diameter, considerably larger than other reported size estimates of IMPs. These results indicate that LAPs represent a novel membrane particle organization, which merits further study. Future developments using this method or further studies to develop alternative membrane preparations may help elucidate membrane protein structure.

Atomic Force Microscopy For Biologists (2nd Edition)

Author : Victor J Morris
Publisher : World Scientific
ISBN 13 : 190897821X
Total Pages : 423 pages
Book Rating : 4.9/5 (89 download)

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Book Synopsis Atomic Force Microscopy For Biologists (2nd Edition) by : Victor J Morris

Download or read book Atomic Force Microscopy For Biologists (2nd Edition) written by Victor J Morris and published by World Scientific. This book was released on 2009-08-11 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the 'natural' conditions usually associated with the light microscope. To biologists, AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems.The second edition of this bestseller has been updated to describe the latest developments in this exciting field, including a brand new chapter on force spectroscopy. The dramatic developments of AFM over the past ten years from a simple imaging tool to the multi-faceted, nano-manipulating technique that it is today are conveyed in a lively and informative narrative, which provides essential reading for students and experienced researchers alike./a

High-Speed Atomic Force Microscopy in Biology

Author : Toshio Ando
Publisher : Springer Nature
ISBN 13 : 3662647850
Total Pages : 327 pages
Book Rating : 4.6/5 (626 download)

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Book Synopsis High-Speed Atomic Force Microscopy in Biology by : Toshio Ando

Download or read book High-Speed Atomic Force Microscopy in Biology written by Toshio Ando and published by Springer Nature. This book was released on 2022-03-23 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first book on high-speed atomic force microscopy (HS-AFM) is intended for students and biologists who want to use HS-AFM in their research. It provides straightforward explanations of the principle and techniques of AFM and HS-AFM. Numerous examples of HS-AFM studies on proteins demonstrate how to apply this new form of microscopy to specific biological problems. Several precautions for successful imaging and the preparation of cantilever tips and substrate surfaces will greatly benefit first-time users of HS-AFM. In turn, the instrumentation techniques detailed in Chapter 4 can be skipped, but will be useful for engineers and scientists who want to develop the next generation of high-speed scanning probe microscopes for biology. The book is intended to facilitate the first-time use of this new technique, and to inspire students and researchers to tackle their own specific biological problems by directly observing dynamic events occurring in the nanoscopic world. Microscopy in biology has recently entered a new era with the advent of high-speed atomic force microscopy (HS-AFM). Unlike optical microscopy, electron microscopy, and conventional slow AFM, it allows us to directly observe biological molecules in physiological environments. Molecular “movies” created using HS-AFM can directly reveal how molecules behave and operate, without the need for subsequent complex analyses and roundabout interpretations. It also allows us to directly monitor morphological change in live cells, and dynamic molecular events occurring on the surfaces of living bacteria and intracellular organelles. As HS-AFM instruments were recently commercialized, in the near future HS-AFM is expected to become a common tool in biology, and will enhance and accelerate our understanding of biological phenomena.

Membrane Proteins Studied by Atomic Force Microscopy Techniques

Author : Patrick Dominic Bosshart
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (781 download)

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Book Synopsis Membrane Proteins Studied by Atomic Force Microscopy Techniques by : Patrick Dominic Bosshart

Download or read book Membrane Proteins Studied by Atomic Force Microscopy Techniques written by Patrick Dominic Bosshart and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Integrated Atomic Force Microscopy Techniques for Analysis of Biomaterials

Author : Laura S. Connelly
Publisher :
ISBN 13 : 9781303971129
Total Pages : 173 pages
Book Rating : 4.9/5 (711 download)

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Book Synopsis Integrated Atomic Force Microscopy Techniques for Analysis of Biomaterials by : Laura S. Connelly

Download or read book Integrated Atomic Force Microscopy Techniques for Analysis of Biomaterials written by Laura S. Connelly and published by . This book was released on 2014 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic Force Microscopy (AFM) is the prominent techniques for structural studies of biological materials in physiological relevant fluidic environments. AFM has been used to resolve the three-dimensional (3D) surface structure of cells, membranes, and proteins structures. Ion channels, formed by membrane proteins, are the key structures that control the activity of all living systems. This dissertation focuses on the structural evaluation of membrane proteins through atomic force microscopy. In Part I, AFM is utilized to study one of the most prominent medical issues facing our society, Alzheimer's Disease (AD). AD is a misfolded protein disease characterized by the accumulation of [Beta]-amyloid (A[Beta]) peptide as senile plaques, progressive neurodegeneration, and memory loss. Recent evidence suggests that AD pathology is linked to the destabilization of cellular ionic homeostasis mediated by toxic channel structures composed of A[Beta] peptides. Selectively engineered sequences of A[Beta] were examined by AFM to elucidate the substructures and thus activity A[Beta] channels. Key residues were evaluated with the intent better understand the exact nature by which these pores conduct electrical and molecular signals, which could aid in identifying potential therapeutic targets for the prevention/treatment of AD. Additionally, AFM was used to analyze brain derived A[Beta] and newly developed pharmacological agents to study membranes and A[Beta]. Part II, presents a novel technology that incorporates electrophysiology into the AFM interface, enabling simultaneous imaging and complementary conductance measurements. The activity of ion channels is studied by various techniques, including patch clamp, free standing lipid bilayers, droplet interface bilayers, and supported lipid bilayers. However, direct correlation with channel structures has remained a challenge. The integrated atomic force microscopy system presented offers a solution to this challenge. The functionality of the system is demonstrated with an Sf9 membrane plaque containing Cx26.

Atomic Force Microscopy in Cell Biology

Author :
Publisher : Academic Press
ISBN 13 : 0080549446
Total Pages : 430 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Atomic Force Microscopy in Cell Biology by :

Download or read book Atomic Force Microscopy in Cell Biology written by and published by Academic Press. This book was released on 2002-05-30 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to cover the history, structure, and application of atomic force microscopy in cell biology. Presented in the clear, well-illustrated style of the Methods in Cell Biology series, it introduces the AFM to its readers and enables them to tap the power and scope of this technology to further their own research. A practical laboratory guide for use of the atomic force and photonic force microscopes, it provides updated technology and methods in force spectroscopy. It is also a comprehensive and easy-to-follow practical laboratory guide for the use of the AFM and PFM in biological research.

Methods for Imaging Cell Membranes

Author : Luca Panconi
Publisher : CRC Press
ISBN 13 : 1003823130
Total Pages : 137 pages
Book Rating : 4.0/5 (38 download)

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Book Synopsis Methods for Imaging Cell Membranes by : Luca Panconi

Download or read book Methods for Imaging Cell Membranes written by Luca Panconi and published by CRC Press. This book was released on 2023-12-21 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: · Measuring membrane protein distributions using single-molecule localisation microscopy (SMLM) · Measuring membrane protein dynamics and diffusion using fluorescence correlation spectroscopy (FCS) · Mapping membrane lipid backing using environmentally sensitive fluorescence probes · Mapping membrane thickness and rigidity using atomic force microscopy · Mapping membrane proteins and the cytoskeleton using electron microscopy

Atomic Force Microscopy

Author : Pier Carlo Braga
Publisher : Springer Science & Business Media
ISBN 13 : 1592596479
Total Pages : 388 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Atomic Force Microscopy by : Pier Carlo Braga

Download or read book Atomic Force Microscopy written by Pier Carlo Braga and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).

Life at the Nanoscale

Author : Yves Dufrene
Publisher : CRC Press
ISBN 13 : 981426797X
Total Pages : 460 pages
Book Rating : 4.8/5 (142 download)

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Book Synopsis Life at the Nanoscale by : Yves Dufrene

Download or read book Life at the Nanoscale written by Yves Dufrene and published by CRC Press. This book was released on 2019-03-28 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.

Atomic Force Microscopy Investigations into Biology

Author : Christopher Frewin
Publisher : BoD – Books on Demand
ISBN 13 : 9535101145
Total Pages : 374 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Atomic Force Microscopy Investigations into Biology by : Christopher Frewin

Download or read book Atomic Force Microscopy Investigations into Biology written by Christopher Frewin and published by BoD – Books on Demand. This book was released on 2012-03-07 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: The atomic force microscope (AFM) has become one of the leading nanoscale measurement techniques for materials science since its creation in the 1980's, but has been gaining popularity in a seemingly unrelated field of science: biology. The AFM naturally lends itself to investigating the topological surfaces of biological objects, from whole cells to protein particulates, and can also be used to determine physical properties such as Young's modulus, stiffness, molecular bond strength, surface friction, and many more. One of the most important reasons for the rise of biological AFM is that you can measure materials within a physiologically relevant environment (i.e. liquids). This book is a collection of works beginning with an introduction to the AFM along with techniques and methods of sample preparation. Then the book displays current research covering subjects ranging from nano-particulates, proteins, DNA, viruses, cellular structures, and the characterization of living cells.

Atomic Force Microscopy in Liquid

Author : Arturo M. Baró
Publisher : John Wiley & Sons
ISBN 13 : 3527327584
Total Pages : 385 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Atomic Force Microscopy in Liquid by : Arturo M. Baró

Download or read book Atomic Force Microscopy in Liquid written by Arturo M. Baró and published by John Wiley & Sons. This book was released on 2012-05-14 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM

Atomic Force Microscopy in Nanobiology

Author : Kunio Takeyasu
Publisher : CRC Press
ISBN 13 : 9814411590
Total Pages : 444 pages
Book Rating : 4.8/5 (144 download)

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Book Synopsis Atomic Force Microscopy in Nanobiology by : Kunio Takeyasu

Download or read book Atomic Force Microscopy in Nanobiology written by Kunio Takeyasu and published by CRC Press. This book was released on 2016-04-19 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in atomic force microscopy (AFM) have been accomplished through various technical and instrumental innovations, including high-resolution and recognition imaging technology under physiological conditions, fast-scanning AFM, and general methods for cantilever modification and force measurement. All these techniques are now highly

Amplitude Modulation Atomic Force Microscopy

Author : Ricardo García
Publisher : John Wiley & Sons
ISBN 13 : 352764394X
Total Pages : 212 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Amplitude Modulation Atomic Force Microscopy by : Ricardo García

Download or read book Amplitude Modulation Atomic Force Microscopy written by Ricardo García and published by John Wiley & Sons. This book was released on 2011-08-24 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Filling a gap in the literature, this book features in-depth discussions on amplitude modulation AFM, providing an overview of the theory, instrumental considerations and applications of the technique in both academia and industry. As such, it includes examples from material science, soft condensed matter, molecular biology, and biophysics, among others. The text is written in such a way as to enable readers from different backgrounds and levels of expertise to find the information suitable for their needs.

Atomic Force Microscopy for Biologists

Author : V. J. Morris
Publisher : World Scientific Publishing Company
ISBN 13 : 9781860941993
Total Pages : 332 pages
Book Rating : 4.9/5 (419 download)

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Book Synopsis Atomic Force Microscopy for Biologists by : V. J. Morris

Download or read book Atomic Force Microscopy for Biologists written by V. J. Morris and published by World Scientific Publishing Company. This book was released on 1999-01-01 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Macromolecules; Interfacial systems; ordered macromoleculles; Cells, tissue and biominerals; STM; SNOM; SICM; SThM; PFM.

Force Microscopy

Author : Bhanu P. Jena
Publisher : John Wiley & Sons
ISBN 13 : 0470007699
Total Pages : 310 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Force Microscopy by : Bhanu P. Jena

Download or read book Force Microscopy written by Bhanu P. Jena and published by John Wiley & Sons. This book was released on 2006-07-11 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: A complete examination of the uses of the atomic force microscope in biology and medicine This cutting-edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function. The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X-ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore--the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers: * Identification of the "porosome" in the growth hormone secreting cell of the pituitary gland * Probing the structural and physical properties of microbial cell surfaces * Scanning probe microscopic characterization of the higher plant cell wall and its components * Case studies of nano drug delivery systems using engineered dendrimers * AFM techniques for studying living cells * Investigating the intermolecular forces of leukocyte adhesion molecules * Protein-protein interactions * Micromechanical properties of lipid bilayers and vesicles The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine. This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it provide the theory, but also practical considerations such as the selection of the right tools and approach.

Atomic Force Microscopy

Author : Nuno C. Santos
Publisher : Humana Press
ISBN 13 : 9781493988938
Total Pages : 372 pages
Book Rating : 4.9/5 (889 download)

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Book Synopsis Atomic Force Microscopy by : Nuno C. Santos

Download or read book Atomic Force Microscopy written by Nuno C. Santos and published by Humana Press. This book was released on 2018-10-30 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.

Correlative Imaging

Author : Paul Verkade
Publisher : John Wiley & Sons
ISBN 13 : 1119086450
Total Pages : 245 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Correlative Imaging by : Paul Verkade

Download or read book Correlative Imaging written by Paul Verkade and published by John Wiley & Sons. This book was released on 2019-11-04 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Brings a fresh point of view to the current state of correlative imaging and the future of the field This book provides contributions from international experts on correlative imaging, describing their vision of future developments in the field based on where it is today. Starting with a brief historical overview of how the field evolved, it presents the latest developments in microscopy that facilitate the correlative workflow. It also discusses the need for an ideal correlative probe, applications in proteomic and elemental analysis, interpretation methods, and how correlative imaging can incorporate force microscopy, soft x-ray tomography, and volume electron microscopy techniques. Work on placing individual molecules within cells is also featured. Correlative Imaging: Focusing on the Future offers in-depth chapters on: correlative imaging from an LM perspective; the importance of sample processing for correlative imaging; correlative light and volume EM; correlation with scanning probe microscopies; and integrated microscopy. It looks at: cryo-correlative microscopy; correlative cryo soft X-ray imaging; and array tomography. Hydrated-state correlative imaging in vacuo, correlating data from different imaging modalities, and big data in correlative imaging are also considered. Brings a fresh view to one of the hottest topics within the imaging community: the correlative imaging field Discusses current research and offers expert thoughts on the field’s future developments Presented by internationally-recognized editors and contributors with extensive experience in research and applications Of interest to scientists working in the fields of imaging, structural biology, cell biology, developmental biology, neurobiology, cancer biology, infection and immunity, biomaterials and biomedicine Part of the Wiley–Royal Microscopical Society series Correlative Imaging: Focusing on the Future will appeal to those working in the expanding field of the biosciences, correlative microscopy and related microscopic areas. It will also benefit graduate students working in microscopy, as well as anyone working in the microscopy imaging field in biomedical research.