High Throughput Genetic Analysis And Combinatorial Chiral Separations Based On Capillary Electrophoresis

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High-throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis

Author : Wenwan Zhong
Publisher :
ISBN 13 :
Total Pages : 254 pages
Book Rating : 4.:/5 (568 download)

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Book Synopsis High-throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis by : Wenwan Zhong

Download or read book High-throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis written by Wenwan Zhong and published by . This book was released on 2003 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis offers many advantages over conventional analytical methods, such as speed, simplicity, high resolution, low cost, and small sample consumption, especially for the separation of enantiomers. However, chiral method developments still can be time consuming and tedious. We designed a comprehensive enantioseparation protocol employing neutral and sulfated cyclodextrins as chiral selectors for common basic, neutral, and acidic compounds with a 96-capillary array system. By using only four judiciously chosen separation buffers, successful enantioseparations were achieved for 49 out of 54 test compounds spanning a large variety of pKs and structures. Therefore, unknown compounds can be screened in this manner to identify optimal enantioselective conditions in just one run. In addition to superior separation efficiency for small molecules, CE is also the most powerful technique for DNA separations. Using the same multiplexed capillary system with UV absorption detection, DNA sequencing of a short template was done without any dye-labels. Two internal standards were utilized to adjust the migration time variations among capillaries, so that the four electropherograms for the A, T, C, G Sanger reactions can be aligned and base calling can be completed with a level of high confidence. The CE separation of DNA can be applied to study differential gene expression as well. Combined with pattern recognition techniques, small variations among electropherograms obtained by the separation of cDNA fragments produced from the total RNA samples of different human tissues can be revealed. These variations reflect the differences in total RNA expression among tissues. Thus, this CE-based approach can serve as an alternative to the DNA array techniques in gene expression analysis.

High-Throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (684 download)

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Book Synopsis High-Throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis by :

Download or read book High-Throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis written by and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) offers many advantages over conventional analytical methods, such as speed, simplicity, high resolution, low cost, and small sample consumption, especially for the separation of enantiomers. However, chiral method developments still can be time consuming and tedious. They designed a comprehensive enantioseparation protocol employing neutral and sulfated cyclodextrins as chiral selectors for common basic, neutral, and acidic compounds with a 96-capillary array system. By using only four judiciously chosen separation buffers, successful enantioseparations were achieved for 49 out of 54 test compounds spanning a large variety of pKs and structures. Therefore, unknown compounds can be screened in this manner to identify optimal enantioselective conditions in just one rn. In addition to superior separation efficiency for small molecules, CE is also the most powerful technique for DNA separations. Using the same multiplexed capillary system with UV absorption detection, the sequence of a short DNA template can be acquired without any dye-labels. Two internal standards were utilized to adjust the migration time variations among capillaries, so that the four electropherograms for the A, T, C, G Sanger reactions can be aligned and base calling can be completed with a high level of confidence. the CE separation of DNA can be applied to study differential gene expression as well. Combined with pattern recognition techniques, small variations among electropherograms obtained by the separation of cDNA fragments produced from the total RNA samples of different human tissues can be revealed. These variations reflect the differences in total RNA expression among tissues. Thus, this Ce-based approach can serve as an alternative to the DNA array techniques in gene expression analysis.

Capillary Electrophoresis in Biotechnology and Environmental Analysis

Author : Hasan Parvez
Publisher : CRC Press
ISBN 13 : 1466563699
Total Pages : 495 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis Capillary Electrophoresis in Biotechnology and Environmental Analysis by : Hasan Parvez

Download or read book Capillary Electrophoresis in Biotechnology and Environmental Analysis written by Hasan Parvez and published by CRC Press. This book was released on 2023-01-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text aims to evaluate the actual impact of high-performance capillary electrophoresis on analytical biotechnology and environmental analysis. The first part of the book presents a survey of present innovations in instrument design and different methods of pre-concentration techniques in order to obtain increased separations at higher sensitivities. The second part contains articles on applications of HPCE to protein and peptide analysis. In the third part, applications of HPCE in the investigation of drug abuse and drug interactions are presented. The last two parts of the book deal with the use of HPCE at low-UV wavelengths and negative-UV absorption. The book should be of interest to those working in HPCE research and applications.

Handbook of Capillary Electrophoresis Applications

Author : H. Shintani
Publisher : Springer Science & Business Media
ISBN 13 : 9400915616
Total Pages : 750 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Handbook of Capillary Electrophoresis Applications by : H. Shintani

Download or read book Handbook of Capillary Electrophoresis Applications written by H. Shintani and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.

Capillary Electrophoresis

Author : Patrick Camilleri
Publisher : CRC Press
ISBN 13 : 9780849391279
Total Pages : 586 pages
Book Rating : 4.3/5 (912 download)

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Book Synopsis Capillary Electrophoresis by : Patrick Camilleri

Download or read book Capillary Electrophoresis written by Patrick Camilleri and published by CRC Press. This book was released on 1997-11-25 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.

Chiral Separations by Capillary Electrophoresis

Author : Ann Van Eeckhaut
Publisher : CRC Press
ISBN 13 : 1420069349
Total Pages : 546 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Chiral Separations by Capillary Electrophoresis by : Ann Van Eeckhaut

Download or read book Chiral Separations by Capillary Electrophoresis written by Ann Van Eeckhaut and published by CRC Press. This book was released on 2009-11-11 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the Fundamentals of Chiral Separation, Available Chiral Selectors, and Numerous Applications of Chiral Separation by Capillary ElectrophoresisSince the 1980s, modern analytical tools have enabled capillary electrophoresis to become a standard part of the chemist's toolkit. With contributions from international experts, Chiral Separations by

Capillary Electrophoresis in Chiral Analysis

Author : Bezhan Chankvetadze
Publisher : John Wiley & Sons
ISBN 13 : 9780471974154
Total Pages : 586 pages
Book Rating : 4.9/5 (741 download)

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Book Synopsis Capillary Electrophoresis in Chiral Analysis by : Bezhan Chankvetadze

Download or read book Capillary Electrophoresis in Chiral Analysis written by Bezhan Chankvetadze and published by John Wiley & Sons. This book was released on 1997-12-29 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: Das weltweite Interesse an der Trennung von Enantiomeren, Spiegelbildern ein und derselben chemischen Verbindung, nimmt zu, da diese Formen beispielsweise verschiedene physiologische Wirkungen hervorrufen können. Eine hochaktuelle Technik der Stofftrennung in diesem Zusammenhang ist die Kapillarelektrophorese, die hier von den theoretischen Grundlagen bis hin zu analytischen Details und Anbieterlisten ausführlich beschrieben wird.

High-throughput Analysis with 96-capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection

Download High-throughput Analysis with 96-capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection PDF Online Free

Author : Gang Xue
Publisher :
ISBN 13 :
Total Pages : 266 pages
Book Rating : 4.:/5 (499 download)

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Book Synopsis High-throughput Analysis with 96-capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection by : Gang Xue

Download or read book High-throughput Analysis with 96-capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection written by Gang Xue and published by . This book was released on 2001 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this research was to improve the fluorescence detection for the multiplexed capillary array electrophoresis, extend its use beyond the genomic analysis, and to develop an integrated micro-sample preparation system for high-throughput DNA sequencing. We first demonstrated multiplexed capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) separations in a 96-capillary array system with laser-induced fluorescence detection. Migration times of four kinds of fluoresceins and six polyaromatic hydrocarbons (PAHs) are normalized to one of the capillaries using two internal standards. The relative standard deviations (RSD) after normalization are 0.6-1.4% for the fluoresceins and 0.1-1.5% for the PAHs. Quantitative calibration of the separations based on peak areas is also performed, again with substantial improvement over the raw data. This opens up the possibility of performing massively parallel separations for high-throughput chemical analysis for process monitoring, combinatorial synthesis, and clinical diagnosis. We further improved the fluorescence detection by step laser scanning. A computer-controlled galvanometer scanner is adapted for scanning a focused laser beam across a 96-capillary array for laser-induced fluorescence detection. The signal at a single photomultiplier tube is temporally sorted to distinguish among the capillaries. The limit of detection for fluorescein is 3 x 10−11M (S/N=3) for 5-mW of total laser power scanned at 4 Hz. The observed cross-talk among capillaries is 0.2%. A simple two-dimensional laser scanning scheme was also proposed with slight modification. 488 nm and 514 nm laser lines from a single Ar laser were alternatively scanned through the array to achieve two-color basecalling for successful high-throughput DNA sequencing. Finally, we addressed the sample preparation for the high-throughput DNA sequencing. An integrated system of nanoreactor for cycle-sequencing reaction coupled with online CZE purification and CGE DNA sequencing is presented. Less than 100 nl of cycle sequencing premixed reagent solution was hydrodynamically injected into a fused-silica capillary (75 [mu]m i.d.) inside a homemade microthermocycler for successful sample preparation, online purification, sequencing separation, and basecalling. The whole system is readily adaptable to automated multiplex operation for DNA sequencing or PCR analysis.

Capillary Electrophoresis of Nucleic Acids

Author : Keith R. Mitchelson
Publisher : Springer Science & Business Media
ISBN 13 : 1592590551
Total Pages : 481 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Capillary Electrophoresis of Nucleic Acids by : Keith R. Mitchelson

Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

High-Throughput Analysis With 96-Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (684 download)

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Book Synopsis High-Throughput Analysis With 96-Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection by :

Download or read book High-Throughput Analysis With 96-Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this research was to improve the fluorescence detection for the multiplexed capillary array electrophoresis, extend its use beyond the genomic analysis, and to develop an integrated micro-sample preparation system for high-throughput DNA sequencing. The authors first demonstrated multiplexed capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) separations in a 96-capillary array system with laser-induced fluorescence detection. Migration times of four kinds of fluoresceins and six polyaromatic hydrocarbons (PAHs) are normalized to one of the capillaries using two internal standards. The relative standard deviations (RSD) after normalization are 0.6-1.4% for the fluoresceins and 0.1-1.5% for the PAHs. Quantitative calibration of the separations based on peak areas is also performed, again with substantial improvement over the raw data. This opens up the possibility of performing massively parallel separations for high-throughput chemical analysis for process monitoring, combinatorial synthesis, and clinical diagnosis. The authors further improved the fluorescence detection by step laser scanning. A computer-controlled galvanometer scanner is adapted for scanning a focused laser beam across a 96-capillary array for laser-induced fluorescence detection. The signal at a single photomultiplier tube is temporally sorted to distinguish among the capillaries. The limit of detection for fluorescein is 3 x 10−11 M (S/N = 3) for 5-mW of total laser power scanned at 4 Hz. The observed cross-talk among capillaries is 0.2%. Advantages include the efficient utilization of light due to the high duty-cycle of step scan, good detection performance due to the reduction of stray light, ruggedness due to the small mass of the galvanometer mirror, low cost due to the simplicity of components, and flexibility due to the independent paths for excitation and emission.

Analysis of Nucleic Acids by Capillary Electrophoresis

Author : Christoph Heller
Publisher : Springer Science & Business Media
ISBN 13 : 3322910156
Total Pages : 318 pages
Book Rating : 4.3/5 (229 download)

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Book Synopsis Analysis of Nucleic Acids by Capillary Electrophoresis by : Christoph Heller

Download or read book Analysis of Nucleic Acids by Capillary Electrophoresis written by Christoph Heller and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.

Handbook of Capillary Electrophoresis, Second Edition

Author : James P. Landers
Publisher : CRC Press
ISBN 13 : 9780849324987
Total Pages : 918 pages
Book Rating : 4.3/5 (249 download)

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Book Synopsis Handbook of Capillary Electrophoresis, Second Edition by : James P. Landers

Download or read book Handbook of Capillary Electrophoresis, Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Practical Capillary Electrophoresis

Author : Robert Weinberger
Publisher : Elsevier
ISBN 13 : 0080539343
Total Pages : 425 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Practical Capillary Electrophoresis by : Robert Weinberger

Download or read book Practical Capillary Electrophoresis written by Robert Weinberger and published by Elsevier. This book was released on 2000-04-18 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. Illustrated with detailed diagrams of electrophoretic phenomena Offers step-by-step methods development schemes Presents techniques for developing quantitative, robust, and precise methods Includes an extensive troubleshooting guide Updates and greatly expands on the first edition-more than 50% of the text is new Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE

Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis

Author : Peter Mikuš
Publisher : BoD – Books on Demand
ISBN 13 : 9535106570
Total Pages : 211 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis by : Peter Mikuš

Download or read book Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis written by Peter Mikuš and published by BoD – Books on Demand. This book was released on 2012-08-29 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt: The scientific monograph by the author Peter Mikus entitled "Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis" provides a comprehensive view on the advanced capillary electrophoresis techniques aimed to current chiral bioanalysis. The advances in the chiral electrophoresis analytical approaches are divided and theoretically described in three sections involving (i) advanced chiral separations for the optimization of chiral resolution (separation mechanisms; electrophoresis techniques in capillary and microchip format; electrophoretic modes such as ITP, CZE/EKC, CEC; chiral additives / pseudophases / phases), (ii) advanced sample preparation for the on-line preconcentration, sample clean-up and analyte derivatization (implementation of electrophoretic effects such as stacking; non-electrophoretic effects such as SPE, chromatography, dialysis; combinations of these effects; multidimensional CE systems; instrumental schemes), (iii) advanced combinations of detection and electrophoresis for the optimization in qualitative and quantitative evaluation (the most important universal as well as selective detection approaches such as absorption and fluorescence spectrophotometry, electrochemical detection, mass spectrometry vs. (i) and/or (ii)). Real analytical potential (benefits and limitations) of these advanced analytical approaches is emphasized by selected performance parameters of the methods and illustrated by many current practical applications including chiral analyses of drugs, their (bio)degradation products and biomarkers in pharmaceutical and biological matrices. The author wishes the readers many inspirations in the creation of new innovative approaches in the field of advanced chiral electrophoresis techniques with the aim to overcome capabilities of the current analytical techniques.

Chiral Separations

Author : Gerald Gübitz
Publisher : Springer Science & Business Media
ISBN 13 : 1592596487
Total Pages : 427 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Chiral Separations by : Gerald Gübitz

Download or read book Chiral Separations written by Gerald Gübitz and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques.

Chiral Separation Methods for Pharmaceutical and Biotechnological Products

Author : Satinder Ahuja
Publisher : John Wiley & Sons
ISBN 13 : 1118097750
Total Pages : 474 pages
Book Rating : 4.1/5 (18 download)

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Book Synopsis Chiral Separation Methods for Pharmaceutical and Biotechnological Products by : Satinder Ahuja

Download or read book Chiral Separation Methods for Pharmaceutical and Biotechnological Products written by Satinder Ahuja and published by John Wiley & Sons. This book was released on 2011-03-31 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discusses chiral separations and offers guidance for selecting the optimum method for desired results Chiral separations represent the most intriguing and, by some measures, most difficultseparations of chemical compounds. This book provides researchers and students an under-standing of chiral separations and offers a convenient route to selecting the best separation method, saving considerable time and cost in product development. Considering chiral separations in the biotechnological and pharmaceutical industries, as well as for food applications, Dr. Ahuja provides insights into a broad range of topics. Opening with a broad overview of chiral separations, regulatory considerations in drug product development, and basic issues in method development, the book: Covers a variety of modern methods such as gas chromatography, high performance liquid chromatography, supercritical fluid chromatography, and capillary electrophoresis Deals with the impact of chirality on the biological activity of small and large molecules Provides detailed information on useful chiral stationary phases (CSPs) for HPLC Includes handy information on selection of an appropriate CSP, including mechanistic studies Offers strategies for fast method development with HPLC, SFC, and CE Discusses preparatory methods utilized in the pharmaceutical industry With in-depth discussions of the current state of the field as well as suggestions to assist future developments, Chiral Separation Methods for Pharmaceutical and Biotechnological Products is an essential text for laboratory investigators, managers, and regulators who are involved in chiral separations in the pharmaceutical industry, as well as students preparing for careers in these fields.

Capillary Electrophoresis

Author : S.F.Y. Li
Publisher : Elsevier
ISBN 13 : 0080858600
Total Pages : 615 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Capillary Electrophoresis by : S.F.Y. Li

Download or read book Capillary Electrophoresis written by S.F.Y. Li and published by Elsevier. This book was released on 1992-07-31 with total page 615 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis. This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful.