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High Speed Dna Sequence Analysis By Matrix Assisted Laser Desorption Mass Spectrometry Final Report For Period February 15 1991 February 14 2001
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Book Synopsis High Speed DNA Sequence Analysis by Matrix-assisted Laser Desorption Mass Spectrometry. Final Report for Period February 15, 1991 - February 14, 2001 by :
Download or read book High Speed DNA Sequence Analysis by Matrix-assisted Laser Desorption Mass Spectrometry. Final Report for Period February 15, 1991 - February 14, 2001 written by and published by . This book was released on 2001 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: This grant had as its focus (i) to develop chemistry and enzymology to permit the enzymatic synthesis of 2' fluoro modified Sanger sequencing reactions, which would be resistant to fragmentation during MALDI process, (ii) to develop rapid MALDI analyses of DNA sequence polymorphisms using Peptide Nucleic Acid (PNA) DNA analogs in conjunction with solid phase chemistry (iii) to study the fundamental mechanisms occurring in the MALDI analysis of nucleic acids.
Book Synopsis Satzungen des Amtes Neuburg und der Gemeinde Hornstorf by :
Download or read book Satzungen des Amtes Neuburg und der Gemeinde Hornstorf written by and published by . This book was released on 2000 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Laser Desorption Mass Spectrometry for DNA Analysis and Sequencing by :
Download or read book Laser Desorption Mass Spectrometry for DNA Analysis and Sequencing written by and published by . This book was released on 1995 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser desorption mass spectrometry has been considered as a potential new method for fast DNA sequencing. Our approach is to use matrix-assisted laser desorption to produce parent ions of DNA segments and a time-of-flight mass spectrometer to identify the sizes of DNA segments. Thus, the approach is similar to gel electrophoresis sequencing using Sanger's enzymatic method. However, gel, radioactive tagging, and dye labeling are not required. In addition, the sequencing process can possibly be finished within a few hundred microseconds instead of hours and days. In order to use mass spectrometry for fast DNA sequencing, the following three criteria need to be satisfied. They are (1) detection of large DNA segments, (2) sensitivity reaching the femtomole region, and (3) mass resolution good enough to separate DNA segments of a single nucleotide difference. It has been very difficult to detect large DNA segments by mass spectrometry before due to the fragile chemical properties of DNA and low detection sensitivity of DNA ions. We discovered several new matrices to increase the production of DNA ions. By innovative design of a mass spectrometer, we can increase the ion energy up to 45 KeV to enhance the detection sensitivity. Recently, we succeeded in detecting a DNA segment with 500 nucleotides. The sensitivity was 100 femtomole. Thus, we have fulfilled two key criteria for using mass spectrometry for fast DNA sequencing. The major effort in the near future is to improve the resolution. Different approaches are being pursued. When high resolution of mass spectrometry can be achieved and automation of sample preparation is developed, the sequencing speed to reach 500 megabases per year can be feasible.
Book Synopsis Molecular Epidemiology by : Paul A. Schulte
Download or read book Molecular Epidemiology written by Paul A. Schulte and published by Academic Press. This book was released on 2012-12-02 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will serve as a primer for both laboratory and field scientists who are shaping the emerging field of molecular epidemiology. Molecular epidemiology utilizes the same paradigm as traditional epidemiology but uses biological markers to identify exposure, disease or susceptibility. Schulte and Perera present the epidemiologic methods pertinent to biological markers. The book is also designed to enumerate the considerations necessary for valid field research and provide a resource on the salient and subtle features of biological indicators.
Book Synopsis Air Emissions from Animal Feeding Operations by : National Research Council
Download or read book Air Emissions from Animal Feeding Operations written by National Research Council and published by National Academies Press. This book was released on 2003-04-07 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Air Emissions from Animal Feeding Operations: Current Knowledge, Future Needs discusses the need for the U.S. Environmental Protection Agency to implement a new method for estimating the amount of ammonia, nitrous oxide, methane, and other pollutants emitted from livestock and poultry farms, and for determining how these emissions are dispersed in the atmosphere. The committee calls for the EPA and the U.S. Department of Agriculture to establish a joint council to coordinate and oversee short - and long-term research to estimate emissions from animal feeding operations accurately and to develop mitigation strategies. Their recommendation was for the joint council to focus its efforts first on those pollutants that pose the greatest risk to the environment and public health.
Book Synopsis Quantitative Proteome Analysis by : Kazuhiro Imai
Download or read book Quantitative Proteome Analysis written by Kazuhiro Imai and published by CRC Press. This book was released on 2013-08-05 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and reproducibility. It also concentrates on the effective applications of these methods that resulted in many discoveries of the role of the proteins expressed in living cells and biological fluids. The first part of the book focuses on the description of advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and, especially, reproducibility. The second part of the book focuses on providing concise descriptions of the effective applications of these methods to demonstrate how they have resulted in many important discoveries of the roles of the proteins expressed in living cells.
Book Synopsis Mass Spectrometry for Biotechnology by : Gary Siuzdak
Download or read book Mass Spectrometry for Biotechnology written by Gary Siuzdak and published by Elsevier. This book was released on 1996-02-12 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt: If you investigate biological systems and might use mass spectrometry in your research but need to know more about it, this book is for you. It introduces the fundamental concepts of mass spectrometry and how mass spectrometers work. It also presents recent advancements particularly interesting to bio-researchers in an easy-to-understand manner that does not require extensive background in chemistry, math, or physics. Glossary of basic terms Abundant illustrations Examples of applications Practical tips on using mass spectrometric techniques Useful for peptide, protein, oligonucleotide, and carbohydrate analysis Simplified description of mass spectrometry including: Matrix-Assisted Laser Desorption/Ionization (MALDI) Electrospray Ionization (ESI) Fast Atom/Ion Bombardment (FAB)
Author :U. S. Department Justice Publisher :Createspace Independent Publishing Platform ISBN 13 :9781500674151 Total Pages :0 pages Book Rating :4.6/5 (741 download)
Book Synopsis The Fingerprint by : U. S. Department Justice
Download or read book The Fingerprint written by U. S. Department Justice and published by Createspace Independent Publishing Platform. This book was released on 2014-08-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The idea of The Fingerprint Sourcebook originated during a meeting in April 2002. Individuals representing the fingerprint, academic, and scientific communities met in Chicago, Illinois, for a day and a half to discuss the state of fingerprint identification with a view toward the challenges raised by Daubert issues. The meeting was a joint project between the International Association for Identification (IAI) and West Virginia University (WVU). One recommendation that came out of that meeting was a suggestion to create a sourcebook for friction ridge examiners, that is, a single source of researched information regarding the subject. This sourcebook would provide educational, training, and research information for the international scientific community.
Book Synopsis Advances in Fingerprint Technology by : Ashim K. Datta
Download or read book Advances in Fingerprint Technology written by Ashim K. Datta and published by CRC Press. This book was released on 2001-06-15 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fingerprints constitute one of the most important categories of physical evidence, and it is among the few that can be truly individualized. During the last two decades, many new and exciting developments have taken place in the field of fingerprint science, particularly in the realm of methods for developing latent prints and in the growth of imag
Book Synopsis The Conservation of Cave 85 at the Mogao Grottoes, Dunhuang by : Neville Agnew
Download or read book The Conservation of Cave 85 at the Mogao Grottoes, Dunhuang written by Neville Agnew and published by Getty Publications. This book was released on 2014-02-01 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Mogao Grottoes, a World Heritage Site in northwestern China, are located along the ancient caravan routes—collectively known as the Silk Road—that once linked China with the West. Founded by a Buddhist monk in the late fourth century, Mogao flourished over the following millennium, as monks, local rulers, and travelers commissioned hundreds of cave temples cut into a mile-long rock cliff and adorned them with vibrant murals. More than 490 decorated grottoes remain, containing thousands of sculptures and some 45,000 square meters of wall paintings, making Mogao one of the world’s most significant sites of Buddhist art. In 1997 the Getty Conservation Institute, which had been working with the Dunhuang Academy since 1989, began a case study using the Late–Tang dynasty Cave 85 to develop a methodology that would stabilize the deteriorating wall paintings. This abundantly illustrated volume is the definitive report on the project, which was completed in 2010.
Book Synopsis The Expanding Role of Mass Spectrometry in Biotechnology by : Gary Siuzdak
Download or read book The Expanding Role of Mass Spectrometry in Biotechnology written by Gary Siuzdak and published by . This book was released on 2006 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the basic concepts in mass spectrometry as well as advanced topics including protein identification/protein structural analysis, carbohydrate and oligonucleotide analysis. Topics also include pharmacokinetics, high throughput screening, and the recent development of mass spectrometry in clinical diagnosis.
Book Synopsis Reference Manual on Scientific Evidence by :
Download or read book Reference Manual on Scientific Evidence written by and published by . This book was released on 1994 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biochar for Environmental Management by : Johannes Lehmann
Download or read book Biochar for Environmental Management written by Johannes Lehmann and published by Routledge. This book was released on 2012-05-16 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochar is the carbon-rich product when biomass (such as wood, manure or crop residues) is heated in a closed container with little or no available air. It can be used to improve agriculture and the environment in several ways, and its stability in soil and superior nutrient-retention properties make it an ideal soil amendment to increase crop yields. In addition to this, biochar sequestration, in combination with sustainable biomass production, can be carbon-negative and therefore used to actively remove carbon dioxide from the atmosphere, with major implications for mitigation of climate change. Biochar production can also be combined with bioenergy production through the use of the gases that are given off in the pyrolysis process. This book is the first to synthesize the expanding research literature on this topic. The book's interdisciplinary approach, which covers engineering, environmental sciences, agricultural sciences, economics and policy, is a vital tool at this stage of biochar technology development. This comprehensive overview of current knowledge will be of interest to advanced students, researchers and professionals in a wide range of disciplines.
Book Synopsis Data Mining for Genomics and Proteomics by : Darius M. Dziuda
Download or read book Data Mining for Genomics and Proteomics written by Darius M. Dziuda and published by John Wiley & Sons. This book was released on 2010-07-16 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Data Mining for Genomics and Proteomics uses pragmatic examples and a complete case study to demonstrate step-by-step how biomedical studies can be used to maximize the chance of extracting new and useful biomedical knowledge from data. It is an excellent resource for students and professionals involved with gene or protein expression data in a variety of settings.
Book Synopsis A Handbook for DNA-Encoded Chemistry by : Robert A. Goodnow, Jr.
Download or read book A Handbook for DNA-Encoded Chemistry written by Robert A. Goodnow, Jr. and published by John Wiley & Sons. This book was released on 2014-04-28 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively describes the development and practice of DNA-encoded library synthesis technology. Together, the chapters detail an approach to drug discovery that offers an attractive addition to the portfolio of existing hit generation technologies such as high-throughput screening, structure-based drug discovery and fragment-based screening. The book: Provides a valuable guide for understanding and applying DNA-encoded combinatorial chemistry Helps chemists generate and screen novel chemical libraries of large size and quality Bridges interdisciplinary areas of DNA-encoded combinatorial chemistry – synthetic and analytical chemistry, molecular biology, informatics, and biochemistry Shows medicinal and pharmaceutical chemists how to efficiently broaden available “chemical space” for drug discovery Provides expert and up-to-date summary of reported literature for DNA-encoded and DNA-directed chemistry technology and methods
Book Synopsis Molecular Biotechnology by : Bernard R. Glick
Download or read book Molecular Biotechnology written by Bernard R. Glick and published by . This book was released on 1998 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.
Book Synopsis Next Generation Sequencing Technologies and Challenges in Sequence Assembly by : Sara El-Metwally
Download or read book Next Generation Sequencing Technologies and Challenges in Sequence Assembly written by Sara El-Metwally and published by Springer Science & Business. This book was released on 2014-04-19 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.
