High Pressure Liquid Chromatography

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High Performance Liquid Chromatography

Author : W.J. Lough
Publisher : CRC Press
ISBN 13 : 9780751400762
Total Pages : 292 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis High Performance Liquid Chromatography by : W.J. Lough

Download or read book High Performance Liquid Chromatography written by W.J. Lough and published by CRC Press. This book was released on 1995-09-30 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Practical High-Performance Liquid Chromatography

Author : Veronika R. Meyer
Publisher : John Wiley & Sons
ISBN 13 : 0470682183
Total Pages : 434 pages
Book Rating : 4.4/5 (76 download)

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Book Synopsis Practical High-Performance Liquid Chromatography by : Veronika R. Meyer

Download or read book Practical High-Performance Liquid Chromatography written by Veronika R. Meyer and published by John Wiley & Sons. This book was released on 2010-05-17 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." - American Journal of Therapeutics

High Performance Liquid Chromatography

Author : Mary T. Gilbert
Publisher : Elsevier
ISBN 13 : 1483281299
Total Pages : 397 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis High Performance Liquid Chromatography by : Mary T. Gilbert

Download or read book High Performance Liquid Chromatography written by Mary T. Gilbert and published by Elsevier. This book was released on 2013-10-22 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Ultra-High Performance Liquid Chromatography and Its Applications

Author : Q. Alan Xu
Publisher : John Wiley & Sons
ISBN 13 : 1118533984
Total Pages : 342 pages
Book Rating : 4.1/5 (185 download)

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Book Synopsis Ultra-High Performance Liquid Chromatography and Its Applications by : Q. Alan Xu

Download or read book Ultra-High Performance Liquid Chromatography and Its Applications written by Q. Alan Xu and published by John Wiley & Sons. This book was released on 2013-04-01 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explores both the benefits and limitations of new UHPLC technology High performance liquid chromatography (HPLC) has been widely used in analytical chemistry and biochemistry to separate, identify, and quantify compounds for decades. The science of liquid chromatography, however, was revolutionized a few years ago with the advent of ultra-high performance liquid chromatography (UHPLC), which made it possible for researchers to analyze sample compounds with greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and Its Applications enables readers to maximize the performance of UHPLC as well as develop UHPLC methods tailored to their particular research needs. Readers familiar with HPLC methods will learn how to transfer these methods to a UHPLC platform and vice versa. In addition, the book explores a variety of UHPLC applications designed to support research in such fields as pharmaceuticals, food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development and method transfer between HPLC and UHPLC platforms. It then examines practical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drug metabolism and pharmacokinetics studies for traditional Chinese medicine. With its frank discussions of UHPLC's benefits and limitations, Ultra-High Performance Liquid Chromatography and Its Applications equips analytical scientists with the skills and knowledge needed to take full advantage of this new separation technology.

High Performance Liquid Chromatography

Author : Heinz Engelhardt
Publisher : Springer
ISBN 13 :
Total Pages : 274 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis High Performance Liquid Chromatography by : Heinz Engelhardt

Download or read book High Performance Liquid Chromatography written by Heinz Engelhardt and published by Springer. This book was released on 1979 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Introduction to High Performance Liquid Chromatography

Author : R. J. Hamilton
Publisher :
ISBN 13 : 9789400959392
Total Pages : 0 pages
Book Rating : 4.9/5 (593 download)

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Book Synopsis Introduction to High Performance Liquid Chromatography by : R. J. Hamilton

Download or read book Introduction to High Performance Liquid Chromatography written by R. J. Hamilton and published by . This book was released on 1982-06-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

High Performance Liquid Chromatography

Author : Omar Al Sayed Omar
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 3110528959
Total Pages : 501 pages
Book Rating : 4.1/5 (15 download)

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Book Synopsis High Performance Liquid Chromatography by : Omar Al Sayed Omar

Download or read book High Performance Liquid Chromatography written by Omar Al Sayed Omar and published by Walter de Gruyter GmbH & Co KG. This book was released on 2022-02-21 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, modern high-performance liquid chromatography (HPLC) utilization has expanded greatly, especially in the quality control of pharmaceutical products in drug quality control laboratories. This book provides an extensive collection of technical information about HPLC-Columns (physicochemical properties and chromatographic characteristics), from various manufacturers, and helps analysts to decide on the ideal approach for their analysis according to the requirements of drug manufacturers specifi cations and the desired Pharmacopeia. In addition, the authors give practical advice on how to prepare mobile phases, choose a suitable detector, and set up an HPLC analysis. This book is comprehensive for the average professional or technician who plans to work with modern HPLC. This book is useful for most Drug Quality Control Laboratories where modern HPLC is utilized. Following a hands-on approach, the book gives key insights into the pharmaceutical applications of HPLC and the latest requirements of the major regulatory agencies such as ICH, FDA, or USP.

Modern Sample Preparation for Chromatography

Author : Serban C. Moldoveanu
Publisher : Elsevier
ISBN 13 : 0323898203
Total Pages : 698 pages
Book Rating : 4.3/5 (238 download)

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Book Synopsis Modern Sample Preparation for Chromatography by : Serban C. Moldoveanu

Download or read book Modern Sample Preparation for Chromatography written by Serban C. Moldoveanu and published by Elsevier. This book was released on 2021-02-24 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern Sample Preparation for Chromatography, Second Edition explains the principles of sample preparation for chromatographic analysis. A variety of procedures are applied to make real-world samples amenable for chromatographic analysis and to improve results. This book's authors discuss each procedure’s advantages, disadvantages and their applicability to different types of samples, along with their fit for different types of chromatographic analysis. The book contains numerous literature references and examples of sample preparation for different matrices and new sections on green approaches in sample preparation, progress in automation of sample preparation, non-conventional solvents for LLE (ionic liquids, deep eutectic mixtures, and others), and more. Presents numerous techniques applied for sample preparation for chromatographic analysis Provides an up-to-date source of information regarding the progress made in sample preparation for chromatography Describes examples for specific types of matrices, providing a guide for choosing the appropriate sample preparation method for a given analysis

Handbook of Pharmaceutical Analysis by HPLC

Author : Satinder Ahuja
Publisher : Elsevier
ISBN 13 : 0080455182
Total Pages : 679 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Handbook of Pharmaceutical Analysis by HPLC by : Satinder Ahuja

Download or read book Handbook of Pharmaceutical Analysis by HPLC written by Satinder Ahuja and published by Elsevier. This book was released on 2005-02-09 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: High pressure liquid chromatography–frequently called high performance liquid chromatography (HPLC or, LC) is the premier analytical technique in pharmaceutical analysis and is predominantly used in the pharmaceutical industry. Written by selected experts in their respective fields, the Handbook of Pharmaceutical Analysis by HPLC Volume 6, provides a complete yet concise reference guide for utilizing the versatility of HPLC in drug development and quality control. Highlighting novel approaches in HPLC and the latest developments in hyphenated techniques, the book captures the essence of major pharmaceutical applications (assays, stability testing, impurity testing, dissolution testing, cleaning validation, high-throughput screening). A complete reference guide to HPLC Describes best practices in HPLC and offers 'tricks of the trade' in HPLC operation and method development Reviews key HPLC pharmaceutical applications and highlights currents trends in HPLC ancillary techniques, sample preparations, and data handling

HPLC

Author : Toshihiko T Hanai
Publisher : Royal Society of Chemistry
ISBN 13 : 1847551076
Total Pages : 146 pages
Book Rating : 4.8/5 (475 download)

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Book Synopsis HPLC by : Toshihiko T Hanai

Download or read book HPLC written by Toshihiko T Hanai and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: How can these compounds be separated? Why was that method used? These are the two basic questions often asked by students of chromatography. HPLC: A Practical Guide provides the answers, enabling the reader to grasp the concepts of the technique using simple, representative chromatograms. Divided into six chapters, this practical guide covers basic concepts of HPLC; instrumentation; stationary phase materials; eluents; column efficiency; and the influence of physical chemistry on separations. Focusing on the basic considerations such as selection of stationary phase and eluent, rather than specific applications, sections on troubleshooting are also included. Uniquely, the descriptions of chromatographic separations are based on solubility using molecular properties, and solubility parameters are used to analyse the selections of chromatographic mode and column. Presenting the chemistry of liquid chromatography for undergraduate students, this valuable practical guide will also be useful for laboratory staff in industry and academia.

Introduction to Modern Liquid Chromatography

Author : Lloyd R. Snyder
Publisher : John Wiley & Sons
ISBN 13 : 1118210395
Total Pages : 831 pages
Book Rating : 4.1/5 (182 download)

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Book Synopsis Introduction to Modern Liquid Chromatography by : Lloyd R. Snyder

Download or read book Introduction to Modern Liquid Chromatography written by Lloyd R. Snyder and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 831 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

High-Performance Liquid Chromatography

Author : Csaba Horváth
Publisher : Elsevier
ISBN 13 : 1483262022
Total Pages : 343 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis High-Performance Liquid Chromatography by : Csaba Horváth

Download or read book High-Performance Liquid Chromatography written by Csaba Horváth and published by Elsevier. This book was released on 2013-10-22 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis. The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient elution. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a valuable reference material.

High Performance Liquid Chromatography in Pesticide Residue Analysis

Author : Tomasz Tuzimski
Publisher : CRC Press
ISBN 13 : 1498766544
Total Pages : 582 pages
Book Rating : 4.4/5 (987 download)

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Book Synopsis High Performance Liquid Chromatography in Pesticide Residue Analysis by : Tomasz Tuzimski

Download or read book High Performance Liquid Chromatography in Pesticide Residue Analysis written by Tomasz Tuzimski and published by CRC Press. This book was released on 2015-12-01 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure, the chromatographer should possess both the practical and theoretical skills required to perform HPLC experiments correctly and to obtain reliable, repeatable, and reproducible results. Developed to serve as a detailed practical guide, High Performance Liquid Chromatography in Pesticide Residue Analysis is a comprehensive source of information and training on state-of-the-art pesticide residue methods performed with the aid of HPLC. The book presents the pros and cons of HPLC as a flexible and versatile separation and analysis tool with multiple purposes and advantages in investigations of pesticides for food and plant drugs standardization, promotion of health, protection of new herbal medicines, and more.

High Performance Liquid Chromatography

Author : Sandie Lindsay
Publisher : John Wiley & Sons
ISBN 13 : 9780471931157
Total Pages : 368 pages
Book Rating : 4.9/5 (311 download)

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Book Synopsis High Performance Liquid Chromatography by : Sandie Lindsay

Download or read book High Performance Liquid Chromatography written by Sandie Lindsay and published by John Wiley & Sons. This book was released on 1992-07-27 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography is the most powerful of all the chromatographic techniques, often achieving separations and analyses that would be difficult or impossible with other forms of chromatography. This study and training text examines the concepts and techniques used in this field. A selection of literature available from equipment manufacturers is included along with a brief review of some more specialized topics.

Introduction to high performance liquid chromatography

Author : R. Hamilton
Publisher : Springer Science & Business Media
ISBN 13 : 9400959389
Total Pages : 256 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Introduction to high performance liquid chromatography by : R. Hamilton

Download or read book Introduction to high performance liquid chromatography written by R. Hamilton and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition of this book the major advances have been in column packings, where over ninety per cent of separations are now performed using chemically bonded microparticulate packings, and in instrumentation. The use of microprocessor control has brought about a rationalization of mobile phase delivery systems and in detectors, the introduction of electrochemical and spectrophotometric detection other than in the ultra-violet region, has widened the field of applications and the sensitivity of the technique. The use of ion pair chromatography has increased at the expense of ion-exchange and this together with the improvements in detectors has greatly increased the appli cation of the technique in the biomedical field. These advances are described together with the established methods to enable the beginner to carry out a satisfactory separation and to gain the experience necessary for the full exploitation of the technique. R. J. Hamilton P. A. Sewell Liverpool,1981 1 Introduction to high performance liquid chromatography 1. 1 Introduction Chromatography in its many forms is widely used as a separative and an analytical technique. Gas chromatography since its introduction by James and Martin [1] has been pre-eminent in the field. Uquid chromatography in the of paper, thin-layer, ion-exchange, and exclusion (gel permeation and gel form filtration) chromatography had not been able to achieve the same success, mainly because of the poor efficiences and the long analysis times arising from the low mobile phase flow rates.

HPLC Method Development for Pharmaceuticals

Author : Satinder Ahuja
Publisher : Elsevier
ISBN 13 : 0080554199
Total Pages : 532 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis HPLC Method Development for Pharmaceuticals by : Satinder Ahuja

Download or read book HPLC Method Development for Pharmaceuticals written by Satinder Ahuja and published by Elsevier. This book was released on 2011-09-21 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: High pressure, or high performance, liquid chromatography (HPLC) is the method of choice for checking purity of new drug candidates, monitoring changes during scale up or revision of synthetic procedures, evaluating new formulations, and running control/assurance of the final drug product. HPLC Method Development for Pharmaceuticals provides an extensive overview of modern HPLC method development that addresses these unique concerns. Includes a review and update of the current state of the art and science of HPLC, including theory, modes of HPLC, column chemistry, retention mechanisms, chiral separations, modern instrumentation (including ultrahigh-pressure systems), and sample preparation. Emphasis has been placed on implementation in a pharmaceutical setting and on providing a practical perspective. HPLC Method Development for Pharmaceuticals is intended to be particularly useful for both novice and experienced HPLC method development chemists in the pharmaceutical industry and for managers who are seeking to update their knowledge. Covers the requirements for HPLC in a pharmaceutical setting including strategies for software and hardware validation to allow for use in a regulated laboratory Provides an overview of the pharmaceutical development process (clinical phases, chemical and pharmaceutical development activities) Discusses how HPLC is used in each phase of pharmaceutical development and how methods are developed to support activities in each phase

Practical High-Performance Liquid Chromatography

Author : Veronika R. Meyer
Publisher : John Wiley & Sons
ISBN 13 : 1118681347
Total Pages : 342 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Practical High-Performance Liquid Chromatography by : Veronika R. Meyer

Download or read book Practical High-Performance Liquid Chromatography written by Veronika R. Meyer and published by John Wiley & Sons. This book was released on 2013-03-25 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “...provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories...a valuable introduction." - American Journal of Therapeutics