Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition

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Publisher : CRC Press
ISBN 13 : 9780849308154
Total Pages : 604 pages
Book Rating : 4.3/5 (81 download)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition by : Leland J. Cseke

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition written by Leland J. Cseke and published by CRC Press. This book was released on 2003-11-24 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Handbook of Molecular and Cellular Methods in Biology and Medicine

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Publisher :
ISBN 13 : 9780429214561
Total Pages : 580 pages
Book Rating : 4.2/5 (145 download)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine by :

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by and published by . This book was released on 2004 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Handbook of Molecular and Cellular Methods in Biology and Medicine, Third Edition

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Author :
Publisher : CRC Press
ISBN 13 : 1420069381
Total Pages : 739 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine, Third Edition by : Leland J. Cseke

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine, Third Edition written by Leland J. Cseke and published by CRC Press. This book was released on 2011-12-12 with total page 739 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.

Handbook of Molecular and Cellular Methods in Biology and Medicine

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Publisher : CRC Press
ISBN 13 : 9781135517243
Total Pages : 496 pages
Book Rating : 4.5/5 (172 download)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine by : Donghern Kim

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Donghern Kim and published by CRC Press. This book was released on 2004-11-23 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Handbook of Molecular and Cellular Methods in Biology and Medicine

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Publisher : CRC Press
ISBN 13 : 9780849325113
Total Pages : 496 pages
Book Rating : 4.3/5 (251 download)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine by : Donghern Kim

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Donghern Kim and published by CRC Press. This book was released on 1995-05-15 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Handbook of Molecular and Cellular Methods in Biology and Medicine

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Publisher : CRC Press
ISBN 13 : 1439881952
Total Pages : 735 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine by : Leland J. Cseke

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Leland J. Cseke and published by CRC Press. This book was released on 2016-04-19 with total page 735 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology

Molecular Biomethods Handbook

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Publisher : Springer
ISBN 13 : 1592596428
Total Pages : 708 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Molecular Biomethods Handbook by : Ralph Rapley

Download or read book Molecular Biomethods Handbook written by Ralph Rapley and published by Springer. This book was released on 2007-10-09 with total page 708 pages. Available in PDF, EPUB and Kindle. Book excerpt: An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications.

Handbook of Physics in Medicine and Biology

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Publisher : CRC Press
ISBN 13 : 142007525X
Total Pages : 550 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Handbook of Physics in Medicine and Biology by : Robert Splinter

Download or read book Handbook of Physics in Medicine and Biology written by Robert Splinter and published by CRC Press. This book was released on 2010-04-05 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: In considering ways that physics has helped advance biology and medicine, what typically comes to mind are the various tools used by researchers and clinicians. We think of the optics put to work in microscopes, endoscopes, and lasers; the advanced diagnostics permitted through magnetic, x-ray, and ultrasound imaging; and even the nanotools, that a

Molecular Biomethods Handbook

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Publisher : Humana Press
ISBN 13 : 9781603273701
Total Pages : 1124 pages
Book Rating : 4.2/5 (737 download)

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Book Synopsis Molecular Biomethods Handbook by : John M. Walker

Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Humana Press. This book was released on 2008-08-25 with total page 1124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.

Experimental Design for Biologists

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Publisher : CSHL Press
ISBN 13 : 0879697350
Total Pages : 211 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Experimental Design for Biologists by : David J. Glass

Download or read book Experimental Design for Biologists written by David J. Glass and published by CSHL Press. This book was released on 2007 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt: The effective design of scientific experiments is critical to success, yet graduate students receive very little formal training in how to do it. Based on a well-received course taught by the author, Experimental Design for Biologistsfills this gap. Experimental Design for Biologistsexplains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as “assumption controls†and “experimentalist controls†. Furthermore, there are sections on establishing the experimental system, which include performing critical “system controls†. Should all experimental plans be hypothesis-driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? What color is the sky? How does one get to Carnegie Hall? The answers to these kinds of questions can be found in Experimental Design for Biologists. Written in an engaging manner, the book provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. Experimental Design for Biologistsis an essential source of theory and practical guidance in designing a research plan.

Molecular Biology of the Cell

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ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (564 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2004 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Human Cell Culture Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592598617
Total Pages : 357 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Human Cell Culture Protocols by : Joanna Picot

Download or read book Human Cell Culture Protocols written by Joanna Picot and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Handbook of Epigenetics

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Publisher : Academic Press
ISBN 13 : 0128054778
Total Pages : 684 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Handbook of Epigenetics by : Trygve O Tollefsbol

Download or read book Handbook of Epigenetics written by Trygve O Tollefsbol and published by Academic Press. This book was released on 2017-07-10 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Epigenetics: The New Molecular and Medical Genetics, Second Edition, provides a comprehensive analysis of epigenetics, from basic biology, to clinical application. Epigenetics is considered by many to be the new genetics in that many biological phenomena are controlled, not through gene mutations, but rather through reversible and heritable epigenetic processes. These epigenetic processes range from DNA methylation to prions. The biological processes impacted by epigenetics are vast and encompass effects in lower organisms and humans that include tissue and organ regeneration, X-chromosome inactivation, stem cell differentiation, genomic imprinting, and aging. The first edition of this important work received excellent reviews; the second edition continues its comprehensive coverage adding more current research and new topics based on customer and reader reviews, including new discoveries, approved therapeutics, and clinical trials. From molecular mechanisms and epigenetic technology, to discoveries in human disease and clinical epigenetics, the nature and applications of the science is presented for those with interests ranging from the fundamental basis of epigenetics, to therapeutic interventions for epigenetic-based disorders. - Timely and comprehensive collection of fully up-to-date reviews on epigenetics that are organized into one volume and written by leading figures in the field - Covers the latest advances in many different areas of epigenetics, ranging from basic aspects, to technologies, to clinical medicine - Written at a verbal and technical level that can be understood by scientists and college students - Updated to include new epigenetic discoveries, newly approved therapeutics, and clinical trials

Molecular Biology of the Cell 6E - The Problems Book

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Publisher : Garland Science
ISBN 13 : 1317497279
Total Pages : 984 pages
Book Rating : 4.3/5 (174 download)

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Book Synopsis Molecular Biology of the Cell 6E - The Problems Book by : John Wilson

Download or read book Molecular Biology of the Cell 6E - The Problems Book written by John Wilson and published by Garland Science. This book was released on 2014-11-21 with total page 984 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Problems Book helps students appreciate the ways in which experiments and simple calculations can lead to an understanding of how cells work by introducing the experimental foundation of cell and molecular biology. Each chapter reviews key terms, tests for understanding basic concepts, and poses research-based problems. The Problems Book has be

Clinical Molecular Medicine

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Publisher : Academic Press
ISBN 13 : 0128094427
Total Pages : 607 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Clinical Molecular Medicine by : Dhavendra Kumar

Download or read book Clinical Molecular Medicine written by Dhavendra Kumar and published by Academic Press. This book was released on 2019-11-30 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical Molecular Medicine: Principles and Practice presents the latest scientific advances in molecular and cellular biology, including the development of new and effective drug and biological therapies and diagnostic methods. The book provides medical and biomedical students and researchers with a clear and clinically relevant understanding on the molecular basis of human disease. With an increased focus on new practice concepts, such as stratified, personalized and precision medicine, this book is a valuable and much-needed resource that unites the core principles of molecular biology with the latest and most promising genomic advances. Illustrates the fundamental principles and therapeutic applications of molecular and cellular biology Offers a clinically focused account of molecular heterogeneity Includes comprehensive coverage of many different disorders, including growth and development, cardiovascular, metabolic, skin, blood, digestive, inflammatory, neuropsychiatric disorders, and many more

Regulation of Gene Expression

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Publisher : Springer Science & Business Media
ISBN 13 : 1597452289
Total Pages : 334 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Regulation of Gene Expression by : Gary H. Perdew

Download or read book Regulation of Gene Expression written by Gary H. Perdew and published by Springer Science & Business Media. This book was released on 2008-08-17 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

Molecular Biology

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Publisher : Elsevier
ISBN 13 : 0123785952
Total Pages : 924 pages
Book Rating : 4.1/5 (237 download)

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Book Synopsis Molecular Biology by : David P. Clark

Download or read book Molecular Biology written by David P. Clark and published by Elsevier. This book was released on 2012-03-20 with total page 924 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology, Second Edition, examines the basic concepts of molecular biology while incorporating primary literature from today's leading researchers. This updated edition includes Focuses on Relevant Research sections that integrate primary literature from Cell Press and focus on helping the student learn how to read and understand research to prepare them for the scientific world.The new Academic Cell Study Guide features all the articles from the text with concurrent case studies to help students build foundations in the content while allowing them to make the appropriate connections to the text. Animations provided deal with topics such as protein purification, transcription, splicing reactions, cell division and DNA replication and SDS-PAGE. The text also includes updated chapters on Genomics and Systems Biology, Proteomics, Bacterial Genetics and Molecular Evolution and RNA. An updated ancillary package includes flashcards, online self quizzing, references with links to outside content and PowerPoint slides with images.This text is designed for undergraduate students taking a course in Molecular Biology and upper-level students studying Cell Biology, Microbiology, Genetics, Biology, Pharmacology, Biotechnology, Biochemistry, and Agriculture. - NEW: "Focus On Relevant Research" sections integrate primary literature from Cell Press and focus on helping the student learn how to read and understand research to prepare them for the scientific world - NEW: Academic Cell Study Guide features all articles from the text with concurrent case studies to help students build foundations in the content while allowing them to make the appropriate connections to the text - NEW: Animations provided include topics in protein purification, transcription, splicing reactions, cell division and DNA replication and SDS-PAGE - Updated chapters on Genomics and Systems Biology, Proteomics, Bacterial Genetics and Molecular Evolution and RNA - Updated ancillary package includes flashcards, online self quizzing, references with links to outside content and PowerPoint slides with images - Fully revised art program