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Golden Gate Cloning
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Book Synopsis DNA Cloning and Assembly Methods by : Svein Valla
Download or read book DNA Cloning and Assembly Methods written by Svein Valla and published by Humana Press. This book was released on 2014-01-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination. With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.
Book Synopsis Golden Gate Cloning by : Daniel Schindler
Download or read book Golden Gate Cloning written by Daniel Schindler and published by Springer Nature. This book was released on with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis DNA Cloning and Assembly by : Sunil Chandran
Download or read book DNA Cloning and Assembly written by Sunil Chandran and published by Humana. This book was released on 2021-09-02 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis Glyco-Engineering by : Alexandra Castilho
Download or read book Glyco-Engineering written by Alexandra Castilho and published by Humana. This book was released on 2015-06-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.
Book Synopsis Synthetic Biology by : Christina Smolke
Download or read book Synthetic Biology written by Christina Smolke and published by John Wiley & Sons. This book was released on 2018-02-28 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering. Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.
Book Synopsis Chloroplast Biotechnology by : Pal Maliga
Download or read book Chloroplast Biotechnology written by Pal Maliga and published by Humana. This book was released on 2014-03-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Chloroplast Biotechnology: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco and protocols for plastid transformation in crops and Chlamydomonas and Bryophytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoidance of known pitfalls. Authoritative and practical, Chloroplast Biotechnology: Methods and Protocols seek to aid scientists who study chloroplast molecular biology as well as those interested in applications in agriculture, industrial biotechnology and healthcare.
Book Synopsis The House of the Scorpion by : Nancy Farmer
Download or read book The House of the Scorpion written by Nancy Farmer and published by Simon and Schuster. This book was released on 2013-08-01 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: Newberry Honour Award Winner & National Book Award Winner. Matt is six years old when he discovers that he is different from other children and other people. To most, Matt isn't considered a boy at all, but a beast, dirty and disgusting. But to El Patron, lord of a country called Opium, Matt is the guarantee of eternal life. El Patron loves Matt as he loves himself - for Matt is himself. They share the exact same DNA. As Matt struggles to understand his existence and what that existence truly means, he is threatened by a host of sinister and manipulating characters, from El Patron's power-hungry family to the brain-deadened eejits and mindless slaves that toil Opium's poppy fields. Surrounded by a dangerous army of bodyguards, escape is the only chance Matt has to survive. But even escape is no guarantee of freedom . . . because Matt is marked by his difference in ways that he doesn't even suspect. Praise for The House of Scorpions: 'It's a pleasure to read science fiction that's full of warm, strong characters... that doesn't rely on violence as the solution to complex problems of right and wrong. It's a pleasure to read.' Ursula K. LeGuin 'Fabulous' Diana Wynne Jones Also by Nancy Farmer: The Sea of Trolls Land of the Silver Apples The Islands of the Blessed The Lord of Opium
Book Synopsis Synthetic Metabolic Pathways by : Michael Krogh Jensen
Download or read book Synthetic Metabolic Pathways written by Michael Krogh Jensen and published by Humana Press. This book was released on 2017-11-24 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance. Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Metabolic Pathways: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis Production of Recombinant Proteins by : Gerd Gellissen
Download or read book Production of Recombinant Proteins written by Gerd Gellissen and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example. With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."
Book Synopsis Synthetic DNA by : Randall A. Hughes
Download or read book Synthetic DNA written by Randall A. Hughes and published by Humana. This book was released on 2016-09-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology. Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.
Book Synopsis DNA Cloning: A Hands-on Approach by : Seok-Yong Choi
Download or read book DNA Cloning: A Hands-on Approach written by Seok-Yong Choi and published by Springer. This book was released on 2019-04-17 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Book Synopsis A Practical Guide to Protein Engineering by : Tuck Seng Wong
Download or read book A Practical Guide to Protein Engineering written by Tuck Seng Wong and published by Springer Nature. This book was released on 2020-10-29 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Book Synopsis Double Identity by : Margaret Peterson Haddix
Download or read book Double Identity written by Margaret Peterson Haddix and published by Simon and Schuster. This book was released on 2008-06-20 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: So my only protection is a kindergarten teacher and a ninety-eight-pound female minister....And they don't even believe I'm in danger. As Bethany approaches her thirteenth birthday, her parents begin acting more oddly than usual: Her mother cries constantly, and her father barely lets Bethany out of his sight. Then one morning he hustles the entire family into the car, drives across several state lines -- and leaves Bethany with an aunt she never knew existed. Bethany has no idea what's going on. She's worried that her mom and dad are running from some kind of trouble, but she can't find out because they won't tell her where they are going. Bethany's only clue is a few words she overheard her father tell her aunt Myrlie: "She doesn't know anything about Elizabeth." But Aunt Myrlie won't tell Bethany who Elizabeth is, and she won't explain why people in her small town react to Bethany as if they've seen a ghost. The mystery intensifies when Bethany gets a package from her father containing four different birth certificates from four states, with four different last names -- and thousands of dollars in cash. And when a strange man shows up asking questions, Bethany realizes she's not the only one who's desperate to unravel the secrets of her past. In this exhilarating thriller, Margaret Peterson Haddix crafts a taut story so full of twists and turns, readers will be gripped until the startling conclusion.
Download or read book The Orchid Thief written by Susan Orlean and published by Ballantine Books. This book was released on 2011-07-20 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: NEW YORK TIMES BESTSELLER • A NEW YORK TIMES NOTABLE BOOK A modern classic of personal journalism, The Orchid Thief is Susan Orlean’s wickedly funny, elegant, and captivating tale of an amazing obsession. Determined to clone an endangered flower—the rare ghost orchid Polyrrhiza lindenii—a deeply eccentric and oddly attractive man named John Laroche leads Orlean on an unforgettable tour of America’s strange flower-selling subculture, through Florida’s swamps and beyond, along with the Seminoles who help him and the forces of justice who fight him. In the end, Orlean—and the reader—will have more respect for underdog determination and a powerful new definition of passion. In this new edition, coming fifteen years after its initial publication and twenty years after she first met the “orchid thief,” Orlean revisits this unforgettable world, and the route by which it was brought to the screen in the film Adaptation, in a new retrospective essay. Look for special features inside. Join the Random House Reader’s Circle for author chats and more. Praise for The Orchid Thief “Stylishly written, whimsical yet sophisticated, quirkily detailed and full of empathy . . . The Orchid Thief shows [Orlean’s] gifts in full bloom.”—The New York Times Book Review “Fascinating . . . an engrossing journey [full] of theft, hatred, greed, jealousy, madness, and backstabbing.”—Los Angeles Times “Orlean’s snapshot-vivid, pitch-perfect prose . . . is fast becoming one of our national treasures.”—The Washington Post Book World “Orlean’s gifts [are] her ear for the self-skewing dialogue, her eye for the incongruous, convincing detail, and her Didion-like deftness in description.”—Boston Sunday Globe “A swashbuckling piece of reporting that celebrates some virtues that made America great.”—The Wall Street Journal
Download or read book Night Probe! written by Clive Cussler and published by Bantam. This book was released on 2014-11-11 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the midst of an international crisis, Heidi Milligan, a beautiful, brilliant American naval commander, accidentally discovers an obscure reference to the long-buried North American Treaty, a precedent-shattering secret pact between the United States and Great Britain. The President believes that the treaty offers the single shot at salvation for an energy-starved, economically devastated nation, but the only two copies plummeted into the watery depths of the Atlantic in twin disasters long ago. The original document must be found—and the one American who can do the job is Dirk Pitt. But in London, a daring counterplot is being orchestrated to see that the treaty is never implemented. Brian Shaw, a master spy who has often worked hand in hand with American agents, now confronts his most challenging command. Pitt’s mission: Raise the North American Treaty. Shaw’s mission: Stop Pitt. Praise for Night Probe! and the Dirk Pitt® novels “A rich tale . . . an absorbing, carefully told mystery with plenty of surprises.”—Los Angeles Times “Dirk Pitt is a combination James Bond and Jacques Cousteau.”—New York Daily News
Book Synopsis Accelerated Breeding of Cereal Crops by : Andriy Bilichak
Download or read book Accelerated Breeding of Cereal Crops written by Andriy Bilichak and published by Humana. This book was released on 2022-10-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive collection of methods for plant breeders and researchers working in functional genomics of cereal crops. Chapters detail advances in sequencing of cereal genomes, methods of traditional plant breeding, use of machine learning for genomic selection, random and targeted mutagenesis with CRISPR/Cas9, quantitative proteomics and phenotyping in cereals. Authoritative and cutting-edge, Accelerated Breeding of Cereal Crops aims to be of interest to plant breeders, researchers, postdoctoral fellows, and students working in functional genomics for the development of the next generation of crop plants.
Book Synopsis Mr. Penumbra's 24-Hour Bookstore by : Robin Sloan
Download or read book Mr. Penumbra's 24-Hour Bookstore written by Robin Sloan and published by Harper Collins. This book was released on 2012-10-02 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Great Recession has shuffled Clay Jannon out of his life as a web-design drone, and serendipity, sheer curiosity and the ability to climb a ladder like a monkey have landed him a new gig working the night shift at Mr. Penumbra’s 24-Hour Bookstore. But Clay begins to realize that this store is even more curious than its name suggests. There are only a few customers, but they come in repeatedly and never seem to actually buy anything. Instead they “check out” impossibly obscure volumes from strange corners of the store, all according to some elaborate, long-standing arrangement with the gnomic Mr. Penumbra. The store must be a front for something larger, Clay concludes, and soon he has embarked on a complex analysis of the customers’ behaviour and roped his friends into helping him figure out just what’s going on. But once they take their findings to Mr. Penumbra, they discover the secrets extend far beyond the walls of the bookstore. Evoking both the fairy-tale charm of Haruki Murakami and the enthusiastic novel-of-ideas wizardry of Neal Stephenson or Umberto Eco, Mr. Penumbra’s 24-Hour Bookstore is exactly what it sounds like—an establishment you have to enter and will never want to leave.
