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Genotyping
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Download or read book Genotyping written by Stefan J. White and published by Humana. This book was released on 2016-11-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.
Book Synopsis Genotyping by : Ibrokhim Y. Abdurakhmonov
Download or read book Genotyping written by Ibrokhim Y. Abdurakhmonov and published by Intechopen. This book was released on 2018 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: A rare norovirus (NoV) genotype GII.17 has recently emerged and rapidly became predominant in most East Asian countries in the winters of 2014-2015. In this study, we report the diversity of NoV GII.17 in detail; a total of 646 GII.17 sequences obtained during 1978-2015 were analyzed and subjected to meta-analysis. At least five major recombinant GII.17 clusters were identified. Each recombinant variant group appeared to have emerged following the time order: GII.P4-GII.17 (1978-1990), GII.P16-GII.17 (2001-2004), GII.P13-GII.17 (2004-2010), GII.Pe-GII.17 (2012-2015) and GII.P3-GII.17 (2011-2015). The newly emerged GII.P3-GII.17 variant, which exhibited significant sequence and structure variations, is evolving toward a unique lineage. Our results indicate that circulation of GII.17 appears to change every 3-5 years due to replacement by a newly emerged variant and that the evolution of GII.17 is sequentially promoted by inter-genotype recombination, which contributes to the exchange between non-GII.17 and GII.17 RdRp genes and drives the evolution of GII.17 capsid genes.
Book Synopsis Plant Genotyping by : Jacqueline Batley
Download or read book Plant Genotyping written by Jacqueline Batley and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Genotyping: Methods and Protocols is aimed at plant molecular biologists, geneticists, plant breeders and ecologists who have a target question and need to know the most suitable markers and genotyping system to use.
Book Synopsis Phenotypes and Genotypes by : Florian Frommlet
Download or read book Phenotypes and Genotypes written by Florian Frommlet and published by Springer. This book was released on 2016-02-12 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This timely text presents a comprehensive guide to genetic association, a new and rapidly expanding field that aims to elucidate how our genetic code (genotypes) influences the traits we possess (phenotypes). The book provides a detailed review of methods of gene mapping used in association with experimental crosses, as well as genome-wide association studies. Emphasis is placed on model selection procedures for analyzing data from large-scale genome scans based on specifically designed modifications of the Bayesian information criterion. Features: presents a thorough introduction to the theoretical background to studies of genetic association (both genetic and statistical); reviews the latest advances in the field; illustrates the properties of methods for mapping quantitative trait loci using computer simulations and the analysis of real data; discusses open challenges; includes an extensive statistical appendix as a reference for those who are not totally familiar with the fundamentals of statistics.
Book Synopsis Plant Genotyping II by : Robert J. Henry
Download or read book Plant Genotyping II written by Robert J. Henry and published by CABI. This book was released on 2008 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes some of the developments in the field of Plant Genotyping, focusing on single nucleotide polymorphism (SNPs). This book covers the discovery, analysis and uses of SNPs, and examines other approaches to plant genotyping.
Book Synopsis Plant Genotyping by : Robert J. Henry
Download or read book Plant Genotyping written by Robert J. Henry and published by CABI. This book was released on 2001-08-17 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant genotyping, or DNA fingerprinting of plants, is a technology that has matured and is poised for widespread practical application in the fields of breeding, commerce and research. This book examines the technologies available and their application in the analysis of:Wild plant populationsGermplasm collections Plant breedingContributors include leading research workers in this field from North America, Europe and Australasia.
Book Synopsis The GenoType Diet by : Dr. Peter J. D'Adamo
Download or read book The GenoType Diet written by Dr. Peter J. D'Adamo and published by Harmony. This book was released on 2007-12-26 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: What’s Your GenoType? GenoType 1 The Hunter Tall, thin, and intense, with an overabundance of adrenaline and a fierce, nervous energy that winds down with age, the Hunter was originally the success story of the human species. Vulnerable to systemic burnout when overstressed, the Hunter’s modern challenge is to conserve energy for the long haul. GenoType 2 The Gatherer Full-figured, even when not overweight, the Gatherer struggles with body image in a culture where thin is “in.” An unsuccessful crash dieter with a host of metabolic challenges, the Gatherer becomes a glowing example of health when properly nourished. GenoType 3 The Teacher Strong, sinewy, and stable, with great chemical synchronicity and stamina, the Teacher is built for longevity—given the right diet and lifestyle. This is the genotype of balance, blessed with a tremendous capacity for growth and fulfillment. GenoType 4 The Explorer Muscular and adventurous, the Explorer is a biological problem solver, with an impressive ability to adapt to environmental changes, and a better than average capacity for gene repair. The Explorer’s vulnerability to hormonal imbalances and chemical sensitivities can be overcome with a balanced diet and lifestyle. GenoType 5 The Warrior Long, lean, and healthy in youth, the Warrior is subject to a bodily rebellion in midlife.With the optimal diet and lifestyle, the Warrior can overcome the quick-aging metabolic genes and experience a second, “silver,” age of health. GenoType 6 The Nomad A GenoType of extremes, with a great sensitivity to environmental conditions—especially changes in altitude and barometric pressure, the Nomad is vulnerable to neuromuscular and immune problems. Yet a well-conditioned Nomad has the enviable gift of controlling caloric intake and aging gracefully. The author of the international bestseller Eat Right 4 Your Type again breaks new ground with the first diet plan based on your unique genetic code. With Eat Right 4 Your Type and additional books in the Blood Type Diet® series, Dr. Peter J. D’Adamo pioneered a new, revolutionary approach to dieting—one linked to a person’s blood type. In the GenoType Diet, he takes his groundbreaking research to the next level by identifying six unique genetic types. Whether you are a Hunter, Gatherer, Teacher, Explorer, Warrior, or Nomad, Dr. D’Adamo offers a customized program that compliments your genetic makeup to maximize health and weight loss, as well as prevent or even reverse disease. In simple, concise prose, Dr. D’Adamo explains how a host of environmental factors, including diet and lifestyle, dictate how and when your genes express themselves. He goes on to demonstrate precisely how, with the right tools, you can alter your genetic destiny by turning on the good genes and silencing the bad ones. Your health risks, weight, and life span can all be improved by following The GenoType Diet that’s right for you. Using family history and blood type, as well as simple diagnostic tools like fingerprint analysis, leg length measurements, and dental characteristics, Dr. D’Adamo shows you how to map out your genetic identity and discover which of the six GenoType plans you should follow. Without expensive tests or a visit to the doctor, The GenoType Diet reveals previously hidden genetic strengths and weaknesses and provides a precise diet and lifestyle plan for every individual. Based on the latest and most cutting-edge genetic research, this is a twenty-first-century plan for wellness and weight loss from a renowned healthcare pioneer.
Book Synopsis Evaluation of Genotyping Techniques for Crytosporidium Parvum by : Paul A. Rochelle
Download or read book Evaluation of Genotyping Techniques for Crytosporidium Parvum written by Paul A. Rochelle and published by American Water Works Association. This book was released on 2001 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: A research teams reports on its efforts to develop and implement DNA fingerprinting techniques to characterize and differentiate the parvus species of protozoal parasite that is ubiquitous in rivers and lakes, is relatively resistant to chlorine disinfection at commonly used concentrations, and can prove fatal for immune compromised people who become infected. They evaluate fingerprinting methods based on polymerase chain reaction, determine which method is most suitable for determining strains or isolates within a species, implement the most appropriate molecular method for determining whether the isolates can be differentiated based on its animal or human host or geographical source, and determine the particular source of the protozoa contamination in source water. The report is not indexed. c. Book News Inc.
Book Synopsis Epigenetics by : Benedikt Hallgrimsson Ph.D.
Download or read book Epigenetics written by Benedikt Hallgrimsson Ph.D. and published by Univ of California Press. This book was released on 2011-04-11 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: Illuminating the processes and patterns that link genotype to phenotype, epigenetics seeks to explain features, characters, and developmental mechanisms that can only be understood in terms of interactions that arise above the level of the gene. With chapters written by leading authorities, this volume offers a broad integrative survey of epigenetics. Approaching this complex subject from a variety of perspectives, it presents a broad, historically grounded view that demonstrates the utility of this approach for understanding complex biological systems in development, disease, and evolution. Chapters cover such topics as morphogenesis and organ formation, conceptual foundations, and cell differentiation, and together demonstrate that the integration of epigenetics into mainstream developmental biology is essential for answering fundamental questions about how phenotypic traits are produced.
Book Synopsis Fundamentals of Forensic DNA Typing by : John M. Butler
Download or read book Fundamentals of Forensic DNA Typing written by John M. Butler and published by Academic Press. This book was released on 2009-09-30 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals of Forensic DNA Typing is written with a broad viewpoint. It examines the methods of current forensic DNA typing, focusing on short tandem repeats (STRs). It encompasses current forensic DNA analysis methods, as well as biology, technology and genetic interpretation. This book reviews the methods of forensic DNA testing used in the first two decades since early 1980's, and it offers perspectives on future trends in this field, including new genetic markers and new technologies. Furthermore, it explains the process of DNA testing from collection of samples through DNA extraction, DNA quantitation, DNA amplification, and statistical interpretation. The book also discusses DNA databases, which play an important role in law enforcement investigations. In addition, there is a discussion about ethical concerns in retaining DNA profiles and the issues involved when people use a database to search for close relatives. Students of forensic DNA analysis, forensic scientists, and members of the law enforcement and legal professions who want to know more about STR typing will find this book invaluable. - Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect - Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A.Boxes-- "Data, Notes & Applications" sections throughout - Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key
Book Synopsis Handbook of Statistical Genomics by : David J. Balding
Download or read book Handbook of Statistical Genomics written by David J. Balding and published by John Wiley & Sons. This book was released on 2019-07-09 with total page 1740 pages. Available in PDF, EPUB and Kindle. Book excerpt: A timely update of a highly popular handbook on statistical genomics This new, two-volume edition of a classic text provides a thorough introduction to statistical genomics, a vital resource for advanced graduate students, early-career researchers and new entrants to the field. It introduces new and updated information on developments that have occurred since the 3rd edition. Widely regarded as the reference work in the field, it features new chapters focusing on statistical aspects of data generated by new sequencing technologies, including sequence-based functional assays. It expands on previous coverage of the many processes between genotype and phenotype, including gene expression and epigenetics, as well as metabolomics. It also examines population genetics and evolutionary models and inference, with new chapters on the multi-species coalescent, admixture and ancient DNA, as well as genetic association studies including causal analyses and variant interpretation. The Handbook of Statistical Genomics focuses on explaining the main ideas, analysis methods and algorithms, citing key recent and historic literature for further details and references. It also includes a glossary of terms, acronyms and abbreviations, and features extensive cross-referencing between chapters, tying the different areas together. With heavy use of up-to-date examples and references to web-based resources, this continues to be a must-have reference in a vital area of research. Provides much-needed, timely coverage of new developments in this expanding area of study Numerous, brand new chapters, for example covering bacterial genomics, microbiome and metagenomics Detailed coverage of application areas, with chapters on plant breeding, conservation and forensic genetics Extensive coverage of human genetic epidemiology, including ethical aspects Edited by one of the leading experts in the field along with rising stars as his co-editors Chapter authors are world-renowned experts in the field, and newly emerging leaders. The Handbook of Statistical Genomics is an excellent introductory text for advanced graduate students and early-career researchers involved in statistical genetics.
Book Synopsis Calculations for Molecular Biology and Biotechnology by : Frank H. Stephenson
Download or read book Calculations for Molecular Biology and Biotechnology written by Frank H. Stephenson and published by Academic Press. This book was released on 2010-07-30 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts
Book Synopsis Harnessing Genebanks: High-Throughput Phenotyping and Genotyping of Crop Wild Relatives and Landraces by : Andrés J. Cortés
Download or read book Harnessing Genebanks: High-Throughput Phenotyping and Genotyping of Crop Wild Relatives and Landraces written by Andrés J. Cortés and published by Frontiers Media SA. This book was released on 2023-04-06 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Handbook of Plant Genome Mapping by : Khalid Meksem
Download or read book The Handbook of Plant Genome Mapping written by Khalid Meksem and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: While the complete sequencing of the genomes of model organisms such as a multitude of bacteria and archaea, the yeast Saccharomyces cerevisiae, the worm Caenorhabditis elegans, the fly Drosophila melanogaster, and the mouse and human genomes have received much public attention, the deciphering of plant genomeswas greatly lagging behind. Up to now, only two plant genomes, one of the model plant Arabidopsis thaliana and one of the crop species rice (Oryza sativa) have been sequenced, though a series of other crop genome sequencing projects are underway. Notwithstanding this public bias towards genomics of animals and humans, it is nevertheless of great importance for basic and applied sciences and industries in such diverse fields as agriculture, breeding in particular, evolutionary genetics, biotechnology, and food science to know the composition of crop plant genomes in detail. It is equally crucial for a deeper understanding of the molecular basis of biodiversity and synteny. The Handbook of Genome Mapping: Genetic and Physical Mapping is the first book on the market to cover these hot topics in considerable detail, and is set apart by its combination of genetic and physical mapping. Throughout, each chapter begins with an easy-to-read introduction, also making the book the first reference designed for non-specialists and newcomers, too. In addition to being an outstanding bench work reference, the book is an excellent textbook for learning and teaching genomics, in particular for courses on genome mapping. It also serves as an up-to-date guide for seasoned researchers involved in the genetic and physical mapping of genomes, especially plant genomes.
Book Synopsis Advances and Applications of Cost-Effective, High-Throughput Genotyping Technologies for Sustainable Agriculture by : Nisha Singh
Download or read book Advances and Applications of Cost-Effective, High-Throughput Genotyping Technologies for Sustainable Agriculture written by Nisha Singh and published by Frontiers Media SA. This book was released on 2023-12-28 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in next-generation sequencing-driven mass production of genomic data and various other integrated techniques have considerably broadened and deepened our understanding of living organisms' molecular systems. Because complex quantitative traits are difficult to select due to low heritability, conventional plant breeding relies on phenotypic selection and breeder experience, it takes longer to develop a new, improved variety. For association studies to identify DNA markers linked to these complex traits, genotyping chip arrays allow genotyping of thousands of markers in a short amount of time. Plant breeding consistency and predictability have improved thanks to advances in genomics. NGS technologies bring new tools and concepts that can enhance the precision and efficiency of plant breeding such as cost-effective, high throughput genotyping technologies for sustainable agriculture. These genotyping technologies will be lowering the time and cost of developing high-quality food crops that are stress-resistant while still having a high nutritional value. This Research Topic focuses on recent advancements in NGS-related technologies, mainly the development of cost-effective high-throughput genotyping platforms with a wide range of bioinformatics tools, and possible translational multi-omics applications in crop breeding programs for sustainable agriculture.
Book Synopsis Plant Genotyping: From Traditional Markers to Modern Technologies by : Yuri Shavrukov
Download or read book Plant Genotyping: From Traditional Markers to Modern Technologies written by Yuri Shavrukov and published by Frontiers Media SA. This book was released on 2024-05-09 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: In contrast to the external traits of plants, we cannot directly see the genotypes that comprise the underlying set of genetic material encoding these phenotypic traits. To make genotypes accessible for research and further understanding, various genotyping methods are used. Plant genotyping began with relatively simple and elementary molecular markers, like microsatellites or SSR (Simple Sequence Repeats), which were then followed by DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, and now today’s modern and advanced microchip-DNA technology involving hundreds and thousands of reactions simultaneously.
Book Synopsis Genotype by Environment Interaction by : Manjit S. Kang
Download or read book Genotype by Environment Interaction written by Manjit S. Kang and published by CRC-Press. This book was released on 1996-02-06 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genotype-by-Environment Interaction (GEI) is a prevalent issue among crop farmers, plant breeders, geneticists, and production agronomists. This book brings together contributions from expert plant breeders and quantitative geneticists to better understand the relationship between crop performance and environment. This information can reduce the cost of extensive genotype evaluation by eliminating unnecessary testing sites and by fine-tuning breeding programs. Molecular aspects of GEI are discussed for the first time and key bibliographical references on GEI are included in an appendix.
