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Gene Synthesis
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Download or read book Gene Synthesis written by Jean Peccoud and published by Humana Press. This book was released on 2012-02-10 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.
Book Synopsis Molecular Biology of The Cell by : Bruce Alberts
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Genome Editing and Engineering by : Krishnarao Appasani
Download or read book Genome Editing and Engineering written by Krishnarao Appasani and published by Cambridge University Press. This book was released on 2018-08-23 with total page 535 pages. Available in PDF, EPUB and Kindle. Book excerpt: A complete guide to endonuclease-based genomic engineering, from basic science to application in disease biology and clinical treatment.
Book Synopsis Nucleic Acids in Chemistry and Biology by : G Michael Blackburn
Download or read book Nucleic Acids in Chemistry and Biology written by G Michael Blackburn and published by Royal Society of Chemistry. This book was released on 2015-11-09 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: The structure, function and reactions of nucleic acids are central to molecular biology and are crucial for the understanding of complex biological processes involved. Revised and updated Nucleic Acids in Chemistry and Biology 3rd Edition discusses in detail, both the chemistry and biology of nucleic acids and brings RNA into parity with DNA. Written by leading experts, with extensive teaching experience, this new edition provides some updated and expanded coverage of nucleic acid chemistry, reactions and interactions with proteins and drugs. A brief history of the discovery of nucleic acids is followed by a molecularly based introduction to the structure and biological roles of DNA and RNA. Key chapters are devoted to the chemical synthesis of nucleosides and nucleotides, oligonucleotides and their analogues and to analytical techniques applied to nucleic acids. The text is supported by an extensive list of references, making it a definitive reference source. This authoritative book presents topics in an integrated manner and readable style. It is ideal for graduate and undergraduates students of chemistry and biochemistry, as well as new researchers to the field.
Book Synopsis Synthesis And Applications Of DNA And RNA by : Saran Narang
Download or read book Synthesis And Applications Of DNA And RNA written by Saran Narang and published by Elsevier. This book was released on 2012-12-02 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthesis and Applications of DNA and RNA discusses the significant contributions in the development of synthetic routes to DNA and RNA. This book contains nine chapters that describe the complexities in the chemistry and biology of DNA and RNA. After briefly dealing with the various stages of development in the chemical synthesis of polynucleotides, this book goes on presenting the DNA synthesis on solid supports and through the phosphoramidite method on silica supports. The discussions then shift to the chemical-enzymatic synthesis of expressed genes; the biochemical aspects of chemical syntheses of oligoribonucleotides; and the methods of rapid DNA and RNA sequence analysis. A chapter specifically tackles the protocols of DNA synthesis using double-stranded plasmid DNA as a template. The final chapter deals with the use of oligonucleotides for the identification and isolation of specific gene sequences. This chapter also covers the use oligonucleotides in the detection of human genetic diseases. Biologists, geneticists, and researchers interested in DNA and RNA synthesis will find this work invaluable.
Book Synopsis Synthetic DNA by : Randall A. Hughes
Download or read book Synthetic DNA written by Randall A. Hughes and published by Humana. This book was released on 2016-09-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology. Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.
Book Synopsis Invisible Frontiers by : Stephen S. Hall
Download or read book Invisible Frontiers written by Stephen S. Hall and published by Oxford University Press, USA. This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Author Stephen Hall weaves together the scientific, social and political threads of this story - the fierce rivalry between labs, the fateful clash of egos within labs, the invasion of academia by commerce, the public fears about genetic engineering, the threat of government regulation, and the ultimate triumph of modern biology - to give us an outstanding tale of scientific research."--BOOK JACKET.
Book Synopsis DNA Cloning and Assembly Methods by : Svein Valla
Download or read book DNA Cloning and Assembly Methods written by Svein Valla and published by Humana Press. This book was released on 2014-01-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination. With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.
Book Synopsis Chemical and Enzymatic Synthesis of Gene Fragments by : Hans Günter Gassen
Download or read book Chemical and Enzymatic Synthesis of Gene Fragments written by Hans Günter Gassen and published by . This book was released on 1982 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preface: In the past the chemical and enzymatic synthesis of oligonucleotides of defined sequence had to be left to a few experts. Now, however, with the triester approach, the phosphite method and the solid-support techniques gene fragment synthesis has turned into an easy procedure even for a non-chemist. Due to the elegant chemistry involved, all methods work without sophisticated equipment and are prone to mechanisation and eventual automation. It is hoped that combined chemical-encymatic gene synthesis may become a standard technique in a molecular biology laboratory, such as DNA sequencing or in-vitro recombination of nucleic acides. We omitted chemical RNA synthesis, since this field is developing so rapidly at the moment that one has to refer to the original publications. However, we included enzymatic synthesis of RNA fragments, procedures which already have obtained a high degree of standardisation. Most of the contributions are revised versions of the protocols supplied for the EMBO sponsored course on "Automated Chemical and Enzymic Gene Synthesis", held in Darmstadt, March 21 to April 3, 1982. The protocols were improved on the basis of the experience of 30 student scientists with chemical, biological or medical backgrounds. Previously omitted procedures, such as the wandering spot method for oligonucleotide analysis, were included. In editing the manuscript we encountered problems with the nomenclature of nucleic acid components. In unambiquous cases we favoured a simple description, hoping for example, that oligodeoxynucleotide is always understood to mean oligo-2'-deoxyribonucleotide. This book aims to provide those interested in DNA/RNA research with state-of-the-art methods in the synthesis, purification, and analysis of DNA and RNA fragments. The editors wish to thank the authors for their efforts in preparing manuscripts from the the revised laboratory protocols. We gratefully acknowledge the skill and the patience of Mrs. E. Ronnfeldt in typing the manuscripts. We express our thanks to Verlag Chemie for the friendly and very efficient cooperation.--H.G. Gassen A. Lang--Darmstadt, in July 1982.
Book Synopsis Nucleic Acids in Chemistry and Biology by : G. Michael Blackburn
Download or read book Nucleic Acids in Chemistry and Biology written by G. Michael Blackburn and published by Royal Society of Chemistry. This book was released on 2006 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discussing both the chemistry and biology of nucleic acids, this edition also provides coverage of nucleic acid chemistry and reactions and interactions with proteins and drugs.
Book Synopsis Oligonucleotide Synthesis by : Piet Herdewijn
Download or read book Oligonucleotide Synthesis written by Piet Herdewijn and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Book Synopsis Synthetic Biology by : Christina Smolke
Download or read book Synthetic Biology written by Christina Smolke and published by John Wiley & Sons. This book was released on 2018-02-28 with total page 1600 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering. Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.
Book Synopsis Transgenic Animal Technology by : Carl A. Pinkert
Download or read book Transgenic Animal Technology written by Carl A. Pinkert and published by Newnes. This book was released on 2014-06-17 with total page 715 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date
Download or read book Synthetic Genomics written by Fouad Sabry and published by One Billion Knowledgeable. This book was released on 2022-10-05 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: What Is Synthetic Genomics To manufacture new DNA or complete lifeforms, synthetic genomics, a relatively young subfield of synthetic biology, employs techniques such as genetic alteration on already-existent life forms or artificial gene synthesis. These techniques may be used to create new DNA. How You Will Benefit (I) Insights, and validations about the following topics: Chapter 1: Synthetic genomics Chapter 2: Base pair Chapter 3: Bacterial artificial chromosome Chapter 4: Molecular genetics Chapter 5: Yeast artificial chromosome Chapter 6: DNA synthesis Chapter 7: Site-directed mutagenesis Chapter 8: Xenobiology Chapter 9: Index of molecular biology articles Chapter 10: DNA construct Chapter 11: Genomic library Chapter 12: Fosmid Chapter 13: Artificial gene synthesis Chapter 14: Functional cloning Chapter 15: Mycoplasma laboratorium Chapter 16: Nucleic acid analogue Chapter 17: Molecular cloning Chapter 18: Minimal genome Chapter 19: Clyde A. Hutchison III Chapter 20: Synthetic genomes Chapter 21: No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing (II) Answering the public top questions about synthetic genomics. (III) Real world examples for the usage of synthetic genomics in many fields. (IV) 17 appendices to explain, briefly, 266 emerging technologies in each industry to have 360-degree full understanding of synthetic genomics' technologies. Who This Book Is For Professionals, undergraduate and graduate students, enthusiasts, hobbyists, and those who want to go beyond basic knowledge or information for any kind of synthetic genomics.
Book Synopsis Towards an Inexpensive, High-thoughroughput Method of Automated Gene Synthesis by : Michael John Bassetti
Download or read book Towards an Inexpensive, High-thoughroughput Method of Automated Gene Synthesis written by Michael John Bassetti and published by . This book was released on 2007 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Recombinant University by : Doogab Yi
Download or read book The Recombinant University written by Doogab Yi and published by University of Chicago Press. This book was released on 2015-03-23 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: This title examines the history of biotechnology when it was new, especially when synonymous with recombinant DNA technology. It focuses on the academic community in the San Francisco Bay Area where recombinant DNA technology was developed and adopted as the first major commercial technology for genetic engineering at Stanford in the 1970s. The book argues that biotechnology was initially a hybrid creation of academic and commercial institutions held together by the assumption of a positive relationship between private ownership and the public interest.
Download or read book Human Growth Hormone written by S. Raiti and published by Springer Science & Business Media. This book was released on 2013-11-09 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been ten years since the National Hormone and Pituitary Program (then called the National Pituitary Agency) sponsored a symposium on human growth hormone (hGH). Numerous advances have occurred during this period. This book does not attempt to summarize past achievements. Rather, it deals with the contemporary issues in hGH research. A discussion of the present state of the art, of necessity, includes a review of the past. Some of the topics herein discussed include the following: 1. Growth hormone releasing factor (GRF). In 1973, the growth hormone inhibitory factor (somatostatin) had recently been discovered. The search for a releasing factor in humans led to its discovery not in the pituitary but in a pancreatic tumor that secreted growth hormone. The advances are discussed in this book. The current hope is that GRF will eventually become an effective therapeutic agent for idiopathic hypopituitarism in childhood and adolescence. 2. Biosynthesis of hGR by recombinant DNA technology. Current advances are discussed. Although hGH is not yet an approved drug, it will eventually become one. This will broaden our horizons in terms of hGH effectiveness in disorders other than hypopituitary dwarfism. The current experience with this type of hGH in both the Vnited States and Europe is reviewed by several authors.
