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Author : Paul D. Siebert
Publisher : Eaton Publishing Company/Biotechniques Books
ISBN 13 :
Total Pages : 372 pages
Book Rating : 4.:/5 (89 download)
Download or read book Gene Cloning and Analysis by RT-PCR written by Paul D. Siebert and published by Eaton Publishing Company/Biotechniques Books. This book was released on 1998 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : T. A. Brown
Publisher : John Wiley & Sons
ISBN 13 : 1119640784
Total Pages : 434 pages
Book Rating : 4.1/5 (196 download)
Download or read book Gene Cloning and DNA Analysis written by T. A. Brown and published by John Wiley & Sons. This book was released on 2020-11-23 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within. Gene Cloning and DNA Analysis: An Introduction, 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like: The strategies used by researchers and industry practitioners to assemble genome sequences Next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes Includes the use and application of gene editing strategies Interbreeding between Neanderthals and Homo Sapiens Gene Cloning and DNA Analysis: An Introduction, 8th Edition is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis.
Author : B.C. Schaefer
Publisher : Taylor & Francis
ISBN 13 : 1000943445
Total Pages : 213 pages
Book Rating : 4.0/5 (9 download)
Download or read book Gene Cloning and Analysis written by B.C. Schaefer and published by Taylor & Francis. This book was released on 2023-04-28 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on newly emerging technologies that facilitate the isolation and characterization of genes. The detailed protocols will be useful to the seasoned professional and easily understood by the novice. The vast majority of methods are applic
Author : T. A. Brown
Publisher : John Wiley & Sons
ISBN 13 : 1118685288
Total Pages : 337 pages
Book Rating : 4.1/5 (186 download)
Download or read book Gene Cloning and DNA Analysis written by T. A. Brown and published by John Wiley & Sons. This book was released on 2013-04-25 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "... the book content is elegantly illustrated and well organized in clear-cut chapters and subsections... there is a Further Reading section after each chapter that contains several key references... What is extremely useful, almost every reference is furnished with the short but distinct author's remark." –Journal of Heredity, 2007 (on the previous edition)
Author : Julia Lodge
Publisher : Garland Science
ISBN 13 : 1135728925
Total Pages : 612 pages
Book Rating : 4.1/5 (357 download)
Download or read book Gene Cloning written by Julia Lodge and published by Garland Science. This book was released on 2007-01-24 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.
Author : Dr. RAVISHANKAR B.V.
Publisher : Lulu.com
ISBN 13 : 138715592X
Total Pages : 214 pages
Book Rating : 4.3/5 (871 download)
Download or read book GENE CLONING AND GENOMICS (Principles and Applications) written by Dr. RAVISHANKAR B.V. and published by Lulu.com. This book was released on 2017-09-04 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Deoxyribonucleic acid (DNA) is the genetic material of cells. It carries information in a coded form from cell to cell and from parent to offspring. A gene is a linear array of nucleotides located in a particular position on a particular chromosome that encodes a specific functional product (a protein or RNA molecule). When a gene is active, its information is copied first into another nucleic acid, ribonucleic acid (RNA), which in turn directs the synthesis of the gene products, the specific proteins. This lecture introduces some basic concepts of DNA, proteins, genes and genomes.
Author : Dominic Wong
Publisher : Springer Science & Business Media
ISBN 13 : 0387286799
Total Pages : 227 pages
Book Rating : 4.3/5 (872 download)
Download or read book The ABCs of Gene Cloning written by Dominic Wong and published by Springer Science & Business Media. This book was released on 2007-12-31 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.
Author : Terence A. Brown
Publisher :
ISBN 13 :
Total Pages : 363 pages
Book Rating : 4.:/5 (119 download)
Download or read book Gene Cloning and DNA Analysis written by Terence A. Brown and published by . This book was released on 2001 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of Gene Cloning is a fully revised and updated version of this most popular introductory text on the subject. This exciting new edition retains the basic structure and organisation that has proved successful for the previous three editions. Two major changes in the contents of the book are the introduction of the subject of the polymerase chain reaction (PCR) at the beginning of the book with more emphasis being placed on PCR throughout and the inclusion of a new chapter on genome sequencing and functional analysis.
Author : Valdis Berzins
Publisher : Springer Science & Business Media
ISBN 13 : 3642719651
Total Pages : 146 pages
Book Rating : 4.6/5 (427 download)
Download or read book Basic Cloning Procedures written by Valdis Berzins and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
Author : Bing-Yuan Chen
Publisher : Springer Science & Business Media
ISBN 13 : 1592591779
Total Pages : 429 pages
Book Rating : 4.5/5 (925 download)
Download or read book PCR Cloning Protocols written by Bing-Yuan Chen and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Author : Nicola King
Publisher : Springer Science & Business Media
ISBN 13 : 159259283X
Total Pages : 370 pages
Book Rating : 4.5/5 (925 download)
Download or read book RT-PCR Protocols written by Nicola King and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.
Author : Terence A. Brown
Publisher :
ISBN 13 :
Total Pages : 374 pages
Book Rating : 4.3/5 (91 download)
Download or read book Gene Cloning written by Terence A. Brown and published by . This book was released on 1995 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Terence A. Brown
Publisher :
ISBN 13 :
Total Pages : 386 pages
Book Rating : 4.:/5 (78 download)
Download or read book Gene Cloning and DNA Analysis written by Terence A. Brown and published by . This book was released on 2006 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students, including students of genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject."--Jacket.
Author : Stefan Lorkowski
Publisher : John Wiley & Sons
ISBN 13 : 3527605339
Total Pages : 992 pages
Book Rating : 4.5/5 (276 download)
Download or read book Analysing Gene Expression written by Stefan Lorkowski and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 992 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.
Author : Francois Ferre
Publisher : Springer Science & Business Media
ISBN 13 : 1461241642
Total Pages : 379 pages
Book Rating : 4.4/5 (612 download)
Download or read book Gene Quantification written by Francois Ferre and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.
Author : Desmond S. T. Nicholl
Publisher : Cambridge University Press
ISBN 13 : 9780521004718
Total Pages : 308 pages
Book Rating : 4.0/5 (47 download)
Download or read book An Introduction to Genetic Engineering written by Desmond S. T. Nicholl and published by Cambridge University Press. This book was released on 2002-02-07 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: The author presents a basic introduction to the world of genetic engineering. Copyright © Libri GmbH. All rights reserved.
Author :
Publisher : Elsevier
ISBN 13 : 0124199542
Total Pages : 405 pages
Book Rating : 4.1/5 (241 download)
Download or read book Laboratory Methods in Enzymology: DNA written by and published by Elsevier. This book was released on 2013-09-02 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA
