G Protein Methods And Protocols

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G Protein-Coupled Receptor Screening Assays

Author : Sofia Aires M. Martins
Publisher : Humana
ISBN 13 : 9781071612231
Total Pages : 327 pages
Book Rating : 4.6/5 (122 download)

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Book Synopsis G Protein-Coupled Receptor Screening Assays by : Sofia Aires M. Martins

Download or read book G Protein-Coupled Receptor Screening Assays written by Sofia Aires M. Martins and published by Humana. This book was released on 2022-06-19 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer to the field, the reader will find accessible methods and protocols that cover the latest developments on receptor purification, molecular biology, recombinant engineering, and analytical techniques that enable the real time monitoring of the complex GPCR signaling cascade and identification of potential drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficient drugs.

G Protein-Coupled Receptor--Protein Interactions

Author : Susan R. George
Publisher : Wiley-Liss
ISBN 13 :
Total Pages : 362 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis G Protein-Coupled Receptor--Protein Interactions by : Susan R. George

Download or read book G Protein-Coupled Receptor--Protein Interactions written by Susan R. George and published by Wiley-Liss. This book was released on 2005-03-31 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This exciting volume elucidates how GPCRs organize signal transduction and control intracellular activities. Chapters written by the recognized expert in each respective topic offer state-of-the-art updates on the principles and technology, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.

Protein Cages

Author : Brendan P. Orner
Publisher : Humana
ISBN 13 : 9781493946709
Total Pages : 0 pages
Book Rating : 4.9/5 (467 download)

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Book Synopsis Protein Cages by : Brendan P. Orner

Download or read book Protein Cages written by Brendan P. Orner and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume emphasizes new techniques to help understand protein cages and to apply them to a variety of technologies, highlighting the expertise of researchers based on three continents. Protein cages are currently inspiring diverse scientific disciplines and are therefore at the crossroads of extremely widely-scoped research, which is reflected in the detailed chapters of Protein Cages: Methods and Protocols. From nanomaterials studies and iron particles to computational strategies and Atomic Force Microscopy, the chapters herein collectively provide an introduction to the rich world of protein cage research and specific techniques to understand and exploit this fascinating class of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters begin with an introduction to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Protein Cages: Methods and Protocols will help to inspire and further propel the current multi-disciplinary enthusiasm in studying and discovering new applications for protein cages.

G Protein-Coupled Receptors in Drug Discovery

Author : Wayne R. Leifert
Publisher : Humana Press
ISBN 13 : 9781603273169
Total Pages : 0 pages
Book Rating : 4.2/5 (731 download)

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Book Synopsis G Protein-Coupled Receptors in Drug Discovery by : Wayne R. Leifert

Download or read book G Protein-Coupled Receptors in Drug Discovery written by Wayne R. Leifert and published by Humana Press. This book was released on 2009-06-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The G protein-coupled receptors (GPCRs) and associated peripheral G proteins underpin a multitude of physiological processes. The GPCRs represent one of the largest superfamilies in the human genome and are a significant target for bioactive and drug discovery programs. It is estimated that greater than 50% of all drugs, including those in development, currently target GPCRs. Many of the characterized GPCRs have known ligands; however, approximately 20% of GPCRs are described as orphan GPCRs, apparent GPCRs that share the generic high-level structure charact- istic of GPCRs but whose endogenous ligand is not known. Therefore, it is expected that the field of GPCR drug discovery and development will greatly expand in the coming years with emphasis on new generations of drugs against GPCRs with unique therapeuticuseswhichmayincludedrugssuchasallostericregulators,inverseagonists, and identification of orphan GPCR ligands. AswelearnmoreaboutthemolecularsignalingcascadesfollowingGPCRactivation, we acquire a better appreciation of the complexity of cell signaling and as a result, also acquire a vast array ofnew molecularmethods toinvestigate these andother processes. Thegeneralaimofthisbookistoprovideresearcherswitharangeofprotocolsthatmay be useful in their GPCR drug discovery programs. It is also the basis for the devel- ment of future assays in this field. Therefore, the range of topics covered and the appropriate methodological approaches in GPCR drug discovery are reflected in this book. Itisinterestingtonotethatfuturedirectionsindrugdiscoverywillrequireinput and collaboration from a plethora of fields of research. As such, this book will likely be of interest to scientists involved in such fields as molecular biology, pharmacology, biochemistry, cellular signaling, and bio-nanotechnology.

G-Quadruplex Nucleic Acids

Author : Danzhou Yang
Publisher : Humana
ISBN 13 : 9781493996681
Total Pages : 437 pages
Book Rating : 4.9/5 (966 download)

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Book Synopsis G-Quadruplex Nucleic Acids by : Danzhou Yang

Download or read book G-Quadruplex Nucleic Acids written by Danzhou Yang and published by Humana. This book was released on 2020-09-07 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers the structures, properties, and functions of G-quadruplexes in a wide range of biological disciplines, including therapeutic intervention and biomaterial application. The chapters in this book explore a wide range of vital and new experimental techniques used in the study of G-quadruplexes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, G-Quadruplex DNA: Methods and Protocols is a valuable resource for both novice and experienced researchers who work in biophysics, structural biology, computational biology, biochemistry, and molecular and cell biology, and who want to learn more about the potential roles and effects of G-quadruplex in these fields.

Proteomics

Author : Lucio Comai
Publisher : Humana
ISBN 13 : 9781493967452
Total Pages : 0 pages
Book Rating : 4.9/5 (674 download)

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Book Synopsis Proteomics by : Lucio Comai

Download or read book Proteomics written by Lucio Comai and published by Humana. This book was released on 2017-02-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Protein Self-Assembly

Author : Jennifer J. McManus
Publisher : Humana
ISBN 13 : 9781493996803
Total Pages : 266 pages
Book Rating : 4.9/5 (968 download)

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Book Synopsis Protein Self-Assembly by : Jennifer J. McManus

Download or read book Protein Self-Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.

Immunocytochemical Methods and Protocols

Author : Constance Oliver
Publisher : Humana Press
ISBN 13 : 9781617796821
Total Pages : 416 pages
Book Rating : 4.7/5 (968 download)

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Book Synopsis Immunocytochemical Methods and Protocols by : Constance Oliver

Download or read book Immunocytochemical Methods and Protocols written by Constance Oliver and published by Humana Press. This book was released on 2012-04-06 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing “Notes” provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.

Protein Complex Assembly

Author : Joseph A. Marsh
Publisher : Humana
ISBN 13 : 9781493992775
Total Pages : 506 pages
Book Rating : 4.9/5 (927 download)

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Book Synopsis Protein Complex Assembly by : Joseph A. Marsh

Download or read book Protein Complex Assembly written by Joseph A. Marsh and published by Humana. This book was released on 2019-04-15 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Flow Cytometry Protocols

Author : Teresa S. Hawley
Publisher : Springer Nature
ISBN 13 : 107163738X
Total Pages : 460 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Flow Cytometry Protocols by : Teresa S. Hawley

Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Nature. This book was released on with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:

High Throughput Protein Expression and Purification

Author : Sharon A. Doyle
Publisher : Humana Press
ISBN 13 : 9781617378218
Total Pages : 0 pages
Book Rating : 4.3/5 (782 download)

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Book Synopsis High Throughput Protein Expression and Purification by : Sharon A. Doyle

Download or read book High Throughput Protein Expression and Purification written by Sharon A. Doyle and published by Humana Press. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.

Computational Methods for GPCR Drug Discovery

Author : Alexander Heifetz
Publisher : Humana Press
ISBN 13 : 9781493974641
Total Pages : 436 pages
Book Rating : 4.9/5 (746 download)

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Book Synopsis Computational Methods for GPCR Drug Discovery by : Alexander Heifetz

Download or read book Computational Methods for GPCR Drug Discovery written by Alexander Heifetz and published by Humana Press. This book was released on 2017-11-30 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume looks at modern computational strategies and techniques used in GPCR drug discovery including structure and ligand-based approaches and cheminformatics. The chapters in this book describe how these approaches can be applied to address key drug discovery issues, such as receptor structure modelling, function and dynamics, prediction of protein-water-ligand interactions and binding kinetics, free energy of binding, interconversion between agonists and antagonists, deorphanization of GPCRs, and the discovery of biased and allosteric modulators. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary software and tools, step-by-step, readily reproducible modelling protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and unique,Computational Methods for GPCR Drug Discovery is a valuable resource for structural and molecular biologists, computational and medicinal chemists, pharmacologists, and drug designers.

Protein Blotting and Detection

Author : Biji T. Kurien
Publisher : Humana Press
ISBN 13 : 9781934115732
Total Pages : 588 pages
Book Rating : 4.1/5 (157 download)

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Book Synopsis Protein Blotting and Detection by : Biji T. Kurien

Download or read book Protein Blotting and Detection written by Biji T. Kurien and published by Humana Press. This book was released on 2009-05-15 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "Protein Blotting and Detection: Methods and Protocols", expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.

Allostery

Author : Aron W. Fenton
Publisher : Humana Press
ISBN 13 : 9781617793332
Total Pages : 0 pages
Book Rating : 4.7/5 (933 download)

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Book Synopsis Allostery by : Aron W. Fenton

Download or read book Allostery written by Aron W. Fenton and published by Humana Press. This book was released on 2011-11-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/function. In Allostery: Methods and Protocols, expert researchers in the field provide key techniques to investigate this biological phenomenon. Focusing on heterotropic systems with some coverage of homotropic systems, this volume covers the monitoring of allosteric function, allosteric conformational changes, and allosteric changes in protein dynamics/sub-population distribution, as well as topics such as macromolecular and ligand engineering of allosteric functions and computational aids in the study of allostery. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Allostery: Methods and Protocols aids scientists in continuing to study ligand-induced, through-protein effects on protein function (ligand binding/catalysis), a phenomenon that is well recognized through the history of the life sciences and very poorly understood at the molecular level.

Green Fluorescent Protein

Author : Martin Chalfie
Publisher : John Wiley & Sons
ISBN 13 : 0471739480
Total Pages : 486 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Green Fluorescent Protein by : Martin Chalfie

Download or read book Green Fluorescent Protein written by Martin Chalfie and published by John Wiley & Sons. This book was released on 2005-11-18 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.

Protein Synthesis

Author : Robin Martin
Publisher : Humana
ISBN 13 : 9781617370250
Total Pages : 0 pages
Book Rating : 4.3/5 (72 download)

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Book Synopsis Protein Synthesis by : Robin Martin

Download or read book Protein Synthesis written by Robin Martin and published by Humana. This book was released on 2010-11-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca tions is arguably the most complex task performed by cells. Protein Synthesis collects together methods and protocols covering a range of different approaches towards understanding how the cellular machinery accomplishes this task and how these ftinctions might be harnessed by the biotechnology industry to generate novel and useful proteins. The era in which the components of the translational machinery were being catalogued is over. This volume gathers together protocols that focus on preserving and describing the dynamic function as closely as possible. The need to understand exactly how ribosomes are positioned on messages or where tRNA molecules, translation factors, or control proteins are bound, has been appreciated by many of the authors. Several chapters that explore the fidelity and processivity of translation reflect this belief. Moreover, the fundamental importance of rRNA at the heart of the ribosome is a strong theme in a number of the protocols. These articles include in vitro and in vivo systems from bacterial, fungal, plant, and animal systems. Overall, Protein Synthesis might be characterized by the novelty of the approaches employed to illuminate the inner workings of the protein synthetic machinery as well as by the inventiveness of the attempts to harness these reactions for biotechnological applications.

Membrane Proteomics

Author : Matthew J. Peirce
Publisher : Humana Press
ISBN 13 : 9781617378898
Total Pages : 0 pages
Book Rating : 4.3/5 (788 download)

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Book Synopsis Membrane Proteomics by : Matthew J. Peirce

Download or read book Membrane Proteomics written by Matthew J. Peirce and published by Humana Press. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The membranes surrounding cells and organelles constitute their interface with the local environment. The functions of membrane proteins include cell/cell and cell/extracellular matrix recognition, the reception and transduction of extracellular signals, and the tra- port of proteins, solutes and water molecules. Abnormal membrane protein expression has profound biological effects and may, for example, underlie phenotypic and functional differences between normal and tumour cells. Moreover the accessibility, particularly of plasma proteins traversing the plasma membrane of cells, makes them of particular ut- ity to the therapeutic intervention in disease. Indeed, it is estimated that of all currently licensed pharmaceuticals, approximately 70% target proteins resident in the plasma m- brane. In theory, unbiased technologies such as proteomics have the power to de?ne patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. However, although about 25% of open reading frames in fully sequenced genomes are estimated to encode integral membrane proteins, global analysis of membrane protein expression has proved problematic. Membrane protein analysis poses unique challenges at the level of extraction, solubilization, and separation in particular, and to a lesser extent of identi?cation and quantitation. These challenges have, however, fostered creativity, in- vation, and technical advances, many of which are brought together in Membrane P- teomics.