G Protein Coupled Receptor Genetics

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G Protein-Coupled Receptor Genetics

Author : Craig W. Stevens
Publisher : Humana
ISBN 13 : 9781493963324
Total Pages : 0 pages
Book Rating : 4.9/5 (633 download)

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Book Synopsis G Protein-Coupled Receptor Genetics by : Craig W. Stevens

Download or read book G Protein-Coupled Receptor Genetics written by Craig W. Stevens and published by Humana. This book was released on 2016-09-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: G Protein‐Coupled Receptor Genetics: Research and Methods in the Post‐Genomic Era features practical techniques inspired by the fast moving GPCR field. From powerful bioinformatic tools tracing the evolution of GPCRs, to methods for the cellular transfection of engineered viruses containing GPCRs, to optogenetic techniques that produce light-activated GPCRs in live mice, what was once science fiction is now science fact. This detailed volume includes sections covering genetic mechanisms, a genetic toolbox for GPCR discovery, as well as genetic aspects of G protein-coupled receptors in health and medicine. Written for the Methods in Pharmacology and Toxicology series, this book contains the kind of key implementation advice that encourages successful results in the lab. Authoritative and easy to use, G Protein‐Coupled Receptor Genetics: Research and Methods in the Post‐Genomic Era serves as an ideal guide for researchers aiming to continue our progress in this dynamic and exciting area of study.

Identification and Expression of G Protein-Coupled Receptors

Author : Kevin R. Lynch
Publisher : John Wiley & Sons
ISBN 13 : 9780471194934
Total Pages : 246 pages
Book Rating : 4.1/5 (949 download)

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Book Synopsis Identification and Expression of G Protein-Coupled Receptors by : Kevin R. Lynch

Download or read book Identification and Expression of G Protein-Coupled Receptors written by Kevin R. Lynch and published by John Wiley & Sons. This book was released on 1999-05-10 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen tremendous advances in the study of G protein-coupled receptors (GPCRs), including the molecular cloning and identification of more than 100 hundred GPCR genes. But while GPCRs serve as targets for more than 300 medicines in the modern pharmacopoeia, the shrinking pool of known ligands and the continuing discovery of orphan GPCR genes have underscored the need for new approaches to ligand identification. Identification and Expression of G Protein-Coupled Receptors addresses this new direction in GPCR biochemistry-offering a definitive laboratory bench manual that emphasizes expression over primary cloning strategies. In a series of expert contributions by well-known researchers, this book provides detailed protocols for various expression systems-from bacteria to mammalian cells-as well as straightforward opinions on the advantages and shortcomings of each approach. Topics covered include: * Homology screening and the polymerase chain reaction in the cloning of GPCR genes * Cloning of GPCRs using mammalian cell expression * GPCR informatics and the orphan problem * The use of Xenopus laevis oocytes for the study of GPCRs * Stable expression of GPCRs in mammalian cells * Heterologous expression in primary cell cultures * Expression of GPCR in Escherichia coli * Large scale expression and purification of GPCRs in mammalian cells * High-level expression of GPCRs in the Baculovirus/Sf9 cell expression system * Expression of GPCRs in Drosophila Schneider 2 cells * Methods for genetic analysis and ligand identification using heterologous GPCRs expressed in Saccharomyces cerevisiae Supplemented with numerous photographs and illustrations, Identification and Expression of G Protein-Coupled Receptors is important reading for biochemists, pharmacologists, neuroscientists, structural biologists, and anyone involved in GPCR-based research. It delivers a wealth of useful advice, practical tips, and invaluable insight into trends at the cutting-edge of current research.

Neuromorphic Olfaction

Author : Krishna C. Persaud
Publisher : CRC Press
ISBN 13 : 1439871728
Total Pages : 237 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Neuromorphic Olfaction by : Krishna C. Persaud

Download or read book Neuromorphic Olfaction written by Krishna C. Persaud and published by CRC Press. This book was released on 2016-04-19 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many advances have been made in the last decade in the understanding of the computational principles underlying olfactory system functioning. Neuromorphic Olfaction is a collaboration among European researchers who, through NEUROCHEM (Fp7-Grant Agreement Number 216916)-a challenging and innovative European-funded project-introduce novel computing p

Molecular Biology of G-Protein-Coupled Receptors

Author : M. Brann
Publisher : Springer Science & Business Media
ISBN 13 : 1468467727
Total Pages : 343 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Molecular Biology of G-Protein-Coupled Receptors by : M. Brann

Download or read book Molecular Biology of G-Protein-Coupled Receptors written by M. Brann and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: LESLIE L. IVERSEN The present series of volumes is well timed, as the impact of molecular genetics on pharmacology has been profound, and a comprehensive review of the rapid advances of the past decade is much needed. Since the pioneering work of Dale, Ariens, and others in the early years of this century, much of pharmacology has been founded on the concept of receptors. To begin with, the receptor was conceived of as a "black box," which recognized and transduced the biological effects of neurotransmit ters, hormones, or other biological messengers-and which could also represent a target for man-made drugs. It is only in the last two decades that "molecular pharmacology" has blossomed, first with the advent of radioligand binding techniques and second messenger studies which greatly facilitated the biochemical study of drug-receptor interactions, and latterly with increasing knowledge of the molecular architecture of the receptor proteins themselves. This started with the traditional biochemical approach of isolating and purifying the receptor molecules. This proved to be a task of immense technical difficulty because of the low density of receptors in most biological source tissues, although there were some notable successes, e. g. , the purification of the nicotinic acetylcholine receptor from the electric organ of Torpedo. It was the ap plication of molecular genetics technology during the 1980s, however, which really accelerated progress in this field.

Pharmacogenomics in Drug Discovery and Development

Author : Qing Yan
Publisher : Springer Nature
ISBN 13 : 107162573X
Total Pages : 618 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Pharmacogenomics in Drug Discovery and Development by : Qing Yan

Download or read book Pharmacogenomics in Drug Discovery and Development written by Qing Yan and published by Springer Nature. This book was released on 2022-09-06 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition offers a state-of-the-art and integrative vision of pharmacogenomics by exploring new concepts and practical methodologies focusing on disease treatments, from cancers to cardiovascular and neurodegenerative disorders and more. The collection of these theoretical and experimental approaches facilitates problem-solving by tackling the complexity of personalized drug discovery and development. Written by leading experts in their fields for the highly successful Methods in Molecular Biology series, the book aims to provide across-the-board resources to support the translation of pharmacogenomics into better individualized health care. Authoritative and up-to-date, Pharmacogenomics in Drug Discovery and Development, Third Edition aims to aid researchers in approaching the challenges in pharmacogenomics and personalized medicine with the introduction of these novel ideas and cutting-edge methodologies.

G Protein-Coupled Receptors

Author : Tatsuya Haga
Publisher : CRC Press
ISBN 13 : 1420038273
Total Pages : 336 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis G Protein-Coupled Receptors by : Tatsuya Haga

Download or read book G Protein-Coupled Receptors written by Tatsuya Haga and published by CRC Press. This book was released on 2005-08-04 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a broad base of knowledge of G-protein-coupled receptors. Useful at both the university and industrial levels, this book is of particular interest to those who are developing therapeutic approaches to diseases using drugs that influence receptor activation.

Discovery of Novel G Protein-coupled Receptor Genes Including Human GALR3 Receptor Gene

Author : Marek Sawzdargo
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (133 download)

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Book Synopsis Discovery of Novel G Protein-coupled Receptor Genes Including Human GALR3 Receptor Gene by : Marek Sawzdargo

Download or read book Discovery of Novel G Protein-coupled Receptor Genes Including Human GALR3 Receptor Gene written by Marek Sawzdargo and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The G protein-coupled receptors (GPCRs) form a large superfamily of integral membrane proteins. GPCRs have been implicated in a large number of system functions including behaviour, cognition, drug addiction, and various neuropsychiatric diseases. The research work presented in this thesis describes the discovery of six novel putative GPCR genes and a new human galanin receptor subtype GALR3. The genes encoding GPR40, GPR41, GPR42 and GPR43 were identified using a degenerate PCR strategy together with database searches. The aim in that particular project was to search for novel human galanin receptor subtypes. GPR40, GPR41, GPR42 and GPR43 encoded intronless genes that shared little homology with galanin receptors and occurred downstream from the CD22 gene localized previously to chromosome 19q13.1. Use of a customized search procedure of high throughput genomic sequence (HTGS) GenBank database yielded a DNA sequence encoding a human GALR3 receptor gene. In addition a rat orthologue of GALR3 was isolated. The mRNA distribution of GALR3 in rat and human tissues indicated expression in distinct brain regions and peripheral tissues. The human GALR3 was localized to chromosome 22q12.2-13.1 using fluorescence ' in situ' hybridization (FISH). The same database strategy was successful in identification of GPR52 gene and a pseudogene TGPR53 localized to chromosomes 1q24 and 6p21, respectively.

G Protein-Coupled Receptors

Author : Tiina P. Iismaa
Publisher : Springer Science & Business Media
ISBN 13 : 3662219301
Total Pages : 189 pages
Book Rating : 4.6/5 (622 download)

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Book Synopsis G Protein-Coupled Receptors by : Tiina P. Iismaa

Download or read book G Protein-Coupled Receptors written by Tiina P. Iismaa and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is about the recent advances in the structural and functional characterization of receptors that influence intracellular signalling events through interaction with intracellular GTP-binding proteins (G proteins). Molecular cloning of members of the G protein-coupled receptor superfamily has complemented pharmacological investigations in providing a realization of the structural and functional diversity of these receptors. An increased understanding of the involvement of particular receptor subtypes in normal and pathophysiological processes represents exciting possibilities for the development of highly specific and effective therapeutic agents.

Principles of Endocrinology and Hormone Action

Author : Antonino Belfiore
Publisher : Springer
ISBN 13 : 9783319446745
Total Pages : 0 pages
Book Rating : 4.4/5 (467 download)

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Book Synopsis Principles of Endocrinology and Hormone Action by : Antonino Belfiore

Download or read book Principles of Endocrinology and Hormone Action written by Antonino Belfiore and published by Springer. This book was released on 2018-02-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides comprehensive coverage of the current knowledge of the physiology of the endocrine system and hormone synthesis and release, transport, and action at the molecular and cellular levels. It presents essential as well as in-depth information of value to both medical students and specialists in Endocrinology, Gynecology, Pediatrics, and Internal Medicine. Although it is well established that the endocrine system regulates essential functions involved in growth, reproduction, and homeostasis, it is increasingly being recognized that this complex regulatory system comprises not only hormones secreted by the classic endocrine glands but also hormones and regulatory factors produced by many organs, and involves extensive crosstalk with the neural and immune system. At the same time, our knowledge of the molecular basis of hormone action has greatly improved. Understanding this complexity of endocrine physiology is crucial to prevent endocrine disorders, to improve the sensitivity of our diagnostic tools, and to provide the rationale for pharmacological, immunological, or genetic interventions. It is such understanding that this book is designed to foster.

G Protein-Coupled Receptors in Drug Discovery

Author : Kenneth H. Lundstrom
Publisher : CRC Press
ISBN 13 : 1420028219
Total Pages : 376 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis G Protein-Coupled Receptors in Drug Discovery by : Kenneth H. Lundstrom

Download or read book G Protein-Coupled Receptors in Drug Discovery written by Kenneth H. Lundstrom and published by CRC Press. This book was released on 2005-07-11 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: The broad range of G protein-coupled receptors (GPCRs) encompasses all areas of modern medicine and have an enormous impact on the process of drug development. Using disease-oriented methods to cover everything from screening to expression and crystallization, G Protein-Coupled Receptors in Drug Discovery describes the physiological roles of GPCRs

Discovery and Characterization of Three Genes Encoding G Protein-coupled Receptors

Author : Benjamin P. Jung
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (119 download)

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Book Synopsis Discovery and Characterization of Three Genes Encoding G Protein-coupled Receptors by : Benjamin P. Jung

Download or read book Discovery and Characterization of Three Genes Encoding G Protein-coupled Receptors written by Benjamin P. Jung and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

G Protein-Coupled Receptors

Author : Gabriel Berstein
Publisher : CRC Press
ISBN 13 : 9781420048513
Total Pages : 440 pages
Book Rating : 4.0/5 (485 download)

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Book Synopsis G Protein-Coupled Receptors by : Gabriel Berstein

Download or read book G Protein-Coupled Receptors written by Gabriel Berstein and published by CRC Press. This book was released on 2019-04-24 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering recently developed methods in membrane-bound receptors, this book emphasizes receptor structure and function, knowledge of which is essential to the study of signal transduction. G Protein-Coupled Receptors has culled contributors from domestic and international sources, providing a broad base of knowledge. Some topics covered are the r

G Protein-Coupled Receptors

Author : Jesus Giraldo
Publisher : Royal Society of Chemistry
ISBN 13 : 1849733449
Total Pages : 549 pages
Book Rating : 4.8/5 (497 download)

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Book Synopsis G Protein-Coupled Receptors by : Jesus Giraldo

Download or read book G Protein-Coupled Receptors written by Jesus Giraldo and published by Royal Society of Chemistry. This book was released on 2011-08-16 with total page 549 pages. Available in PDF, EPUB and Kindle. Book excerpt: G protein-coupled receptors (GPCRs) are the largest family of cell-surface receptors, with more than 800 members identified thus far in the human genome. They regulate the function of most cells in the body, and represent approximately 3% of the genes in the human genome. These receptors respond to a wide variety of structurally diverse ligands, ranging from small molecules, such as biogenic amines, nucleotides and ions, to lipids, peptides, proteins, and even light. Ligands (agonists and antagonists) acting on GPCRs are important in the treatment of numerous diseases, including cardiovascular and mental disorders, retinal degeneration, cancer, and AIDS. It is estimated that these receptors represent about one third of the actual identified targets of clinically used drugs. The determination of rhodopsin crystal structure and, more recently, of opsin, 1 and 2 adrenergic and A2A adenosine receptors provides both academia and industry with extremely valuable data for a better understanding of the molecular determinants of receptor function and a more reliable rationale for drug design. GPCR structure and function constitutes a hot topic. The book, which lies between the fields of chemical biology, molecular pharmacology and medicinal chemistry, is divided into three parts. The first part considers what receptor structures tell us about the mechanism of receptor activation. Part II focuses on receptor function. It discusses what the data from biophysical and mutational studies, and the analysis of the interactions of the receptor with ligands and regulator proteins, tell us about the process of signal transduction. The final part, on modelling and simulation, details new insights on the link between structure and mechanism and their implications in drug design.

G Protein-Coupled Receptors in Drug Discovery

Author : Wayne R. Leifert
Publisher : Humana Press
ISBN 13 : 9781603273169
Total Pages : 0 pages
Book Rating : 4.2/5 (731 download)

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Book Synopsis G Protein-Coupled Receptors in Drug Discovery by : Wayne R. Leifert

Download or read book G Protein-Coupled Receptors in Drug Discovery written by Wayne R. Leifert and published by Humana Press. This book was released on 2009-06-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The G protein-coupled receptors (GPCRs) and associated peripheral G proteins underpin a multitude of physiological processes. The GPCRs represent one of the largest superfamilies in the human genome and are a significant target for bioactive and drug discovery programs. It is estimated that greater than 50% of all drugs, including those in development, currently target GPCRs. Many of the characterized GPCRs have known ligands; however, approximately 20% of GPCRs are described as orphan GPCRs, apparent GPCRs that share the generic high-level structure charact- istic of GPCRs but whose endogenous ligand is not known. Therefore, it is expected that the field of GPCR drug discovery and development will greatly expand in the coming years with emphasis on new generations of drugs against GPCRs with unique therapeuticuseswhichmayincludedrugssuchasallostericregulators,inverseagonists, and identification of orphan GPCR ligands. AswelearnmoreaboutthemolecularsignalingcascadesfollowingGPCRactivation, we acquire a better appreciation of the complexity of cell signaling and as a result, also acquire a vast array ofnew molecularmethods toinvestigate these andother processes. Thegeneralaimofthisbookistoprovideresearcherswitharangeofprotocolsthatmay be useful in their GPCR drug discovery programs. It is also the basis for the devel- ment of future assays in this field. Therefore, the range of topics covered and the appropriate methodological approaches in GPCR drug discovery are reflected in this book. Itisinterestingtonotethatfuturedirectionsindrugdiscoverywillrequireinput and collaboration from a plethora of fields of research. As such, this book will likely be of interest to scientists involved in such fields as molecular biology, pharmacology, biochemistry, cellular signaling, and bio-nanotechnology.

Characterization of Novel G Protein-coupled Receptor Genes and the Novel Ligand Apelin

Author : Dennis K. Lee
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (133 download)

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Book Synopsis Characterization of Novel G Protein-coupled Receptor Genes and the Novel Ligand Apelin by : Dennis K. Lee

Download or read book Characterization of Novel G Protein-coupled Receptor Genes and the Novel Ligand Apelin written by Dennis K. Lee and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: G protein-coupled receptors (GPCRs) make up the large family of integral membrane bound proteins which mediate the signaling of extracellular stimuli to intracellular responses via activation of heterotrimeric G proteins and their subsequent interaction with effector proteins. GPCRs have been implicated in a number of physiological functions including behaviour, homeostasis, cognition, appetite, and drug addiction. This thesis describes the molecular characterization of the human and rat genes encoding apelin, the cognate ligand for the APJ receptor. Human and rat DNA sequences encoding apelin were retrieved by a search of the GenbankTM databases, their full length sequences cloned and used as probes for expression analyses. Comparative sequence and tissue distribution analyses revealed both apelin and the APJ receptor to resemble the angiotensin II peptide and the angiotensin II receptors, suggesting roles in similar physiological systems. In addition, a degenerate PCR strategy, database searching and the patent literature revealed DNA sequences encoding novel GPCRs, namely the thyrotropin-releasing hormone receptor TRH-R2, orphan GPCRs GPR54, GPR57, GPR58, GPR61, GPR62 and a pseudogene, WGPR57. With the initial discovery of each of these sequences, full length GPCR-encoding sequences were determined and used for mRNA distribution analyses by northern blot and in situ hybridization. In addition, novel GPCR-encoding genes were localized to chromosomes by fluorescence in situ hybridization (FISH) and expressed for pharmacological characterization.

Molecular Biology of the Cell

Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Adhesion G Protein-coupled Receptors

Author : Tobias Langenhan
Publisher : Springer
ISBN 13 : 3319415239
Total Pages : 409 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Adhesion G Protein-coupled Receptors by : Tobias Langenhan

Download or read book Adhesion G Protein-coupled Receptors written by Tobias Langenhan and published by Springer. This book was released on 2016-11-09 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: Latest research on Adhesion GPCRs has unearthed surprising revelations about the events that govern the signal transduction of these receptor molecules and the cellular and organ requirements for these signals. Unexpected and unprecedented findings suggest that Adhesion GPCRs constitute a group of receptors that sense mechanical stimuli and transcode them into metabotropic signals through the action of a novel activation paradigm. Interdisciplinary efforts transcending many areas of biomedical research including pharmacology, physiology, genetics, cell biology, structural biology, biochemistry and bioinformatics were necessary to unveil these fundamental properties. The scientific leaders in the field that carried this research effort have teamed up here to provide a comprehensive overview of our current understanding, how Adhesion GPCRs signal and how these receptors shape organ structure and function.