Functional Analysis Of Atvamp714 Gene In Arabidopsis

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Functional Analysis of AtVAMP714 Gene in Arabidopsis

Author : Champa Kumari Dehiwala-Liyanage
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (757 download)

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Book Synopsis Functional Analysis of AtVAMP714 Gene in Arabidopsis by : Champa Kumari Dehiwala-Liyanage

Download or read book Functional Analysis of AtVAMP714 Gene in Arabidopsis written by Champa Kumari Dehiwala-Liyanage and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: An activation tagging screen was carried out to identify gain-of-function mutants showing potential auxin defects, with the aim of identifying the new genes regulating plant development. The conjoined (cnj) mutant isolated from the activation tagged screen exhibits duplication of the seedling axis. Sequencing the insertion locus revealed that the activation tag was positioned between ATP-binding protein and vesicle associated membrane protein genes. Cloned mutant locus and expression studies indicated that the vesicle associated membrane protein gene is upregulated in the activation tagging line. The aim of this work is to investigate the function of the vesicle associated membrane protein gene (AtVAMP714), which is a member of an R-SNARE protein family. To determine the expression pattern of AtVAMP714, proAtVAMP714::GUS expression in seedlings was examined. GUS activity was observed in the hypocotyl and roots and the strongest expression was observed in the root vascular tissues. The AtVAMP714 gene is positively auxin regulated. VAMP714::GFP fusion protein localized to Golgi vesicles suggesting it may be involved in the Golgi secretory pathway. Auxin transport levels in roots and shoots found to be greatly reduced in AtVAMP714 overexpressors compared to the wild type plants. Quantitative real-time RT-PCR showed that transcript levels of IAA1 and IAA2 were significantly reduced in AtVAMP714 overexpressors. Immunolocalization of PIN1 and PIN2 showed strong defects in localization. To characterize the development role of VAMP714, SALK null mutants of the AtVAMP714 gene were identified. The mutant phenotype showed an abnormal branching and short root phenotype and PIN1, PIN2 and PIN4 transcript levels were significantly reduced. Dominant negative transgenics of AtVAMP714 were also created as a method of knocking out the function of the protein and F2 generation plants were analysed for developmental defects. Consistent with the above results the dominant negative transgenics showed a short root phenotype with dwarfed, branchy shoots. PIN1 and PIN2 proteins were mislocalized in dominant negative transgenics. The results presented provide evidence for a role of AtVAMP714 in auxin transport through a requirement for correct PIN protein secretion and localization.

Functional Analysis of the SPA Gene Family in Arabidopsis Thaliana

Author : Kirsten Fittinghoff
Publisher :
ISBN 13 :
Total Pages : 82 pages
Book Rating : 4.:/5 (436 download)

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Book Synopsis Functional Analysis of the SPA Gene Family in Arabidopsis Thaliana by : Kirsten Fittinghoff

Download or read book Functional Analysis of the SPA Gene Family in Arabidopsis Thaliana written by Kirsten Fittinghoff and published by . This book was released on 2008 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Functional Analysis of the CRINKLY4 Gene Family in Arabidopsis Thaliana and Viviparous1 Promoter in Zea Mays

Author : Xueyuan Cao
Publisher :
ISBN 13 :
Total Pages : 208 pages
Book Rating : 4.:/5 (716 download)

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Book Synopsis Functional Analysis of the CRINKLY4 Gene Family in Arabidopsis Thaliana and Viviparous1 Promoter in Zea Mays by : Xueyuan Cao

Download or read book Functional Analysis of the CRINKLY4 Gene Family in Arabidopsis Thaliana and Viviparous1 Promoter in Zea Mays written by Xueyuan Cao and published by . This book was released on 2005 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cereal endosperm is an excellent system to study cell differentiation and fate specification. The aleurone of maize is one cell layer and requires continuous positional cues to specify and maintain its identity. A genetic hierarchy is implicated in this process. CRINKLY4 (Cr4), a receptor-like kinase, belongs to cell fate-specifying genes and is required to specify aleurone cell identity. Viviparousl (Vpl), a B3 domain-containing transcription factor, functions late in development to regulate the expression of Cl in aleurone cells. It also plays an important regulatory role in late embryogenesis and seed maturation with abscisic acid (ABA). The Arabidopsis genome encodes ACR4, an ortholog of CR4, and four AtCRRs. ACR4 and AtCRRl and 2 are mainly expressed in developing young tissues. The kinase domain of ACR4 was shown to be a serine/threonine kinase and had ability to phosphorylate the non-functional kinase domain of AtCRR2. T-DNA insertion mutants of acr4 showed a phenotype restricted to the integument and seed coat, while mutants of atcrrs did not show obvious phenotype and triple mutants of acr4, atcrrl and 2 had similar phenotype as acr4, suggesting that Arabidopsis might have a redundant function and the hypothetical redundant function is not encoded by AtCRRs. Vpl functions in ABA regulated processes. Its expression is regulated by ABA, high salinity and osmoticum. The induction of Vpl expression by ABA does not require de novo protein synthesis. The sequence analysis of 958 bp of the Vpl promoter identified a potential ABRC (ABA response complex), which consists a predicted ABRE (ABA response element) and a CEIL (coupling element 1-like) element. Electrophoretic mobility shift assay (EMSA) confirmed that the ABRE and CEIL specifically bound proteins in 20 DAP embryo nuclear protein extracts. ABA or osmoticum induce nuclear protein interaction with the ABRE. The ABRE was also shown to be critical for the induction of Vpl expression by ABA in a transient gene expression experiment. Sequence analysis and EMSA experiments also identified several other binding sites such as: VPP1, AtMYB2 and ZIP1. This provides a starting point to further study the regulation of Vpl expression.

Plant signaling: Understanding the molecular crosstalk

Author : Khalid Rehman Hakeem
Publisher : Springer
ISBN 13 : 9788132234791
Total Pages : 0 pages
Book Rating : 4.2/5 (347 download)

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Book Synopsis Plant signaling: Understanding the molecular crosstalk by : Khalid Rehman Hakeem

Download or read book Plant signaling: Understanding the molecular crosstalk written by Khalid Rehman Hakeem and published by Springer. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant signalling has emerged as an integrated field which has become indispensable in recent times to study any biological process. Over the last decade, an enormous amount of information has been generated in this field and the advances in information technology gave birth to bioinformatics which has helped greatly in managing the galaxy of information. It is now possible to view the different information’s in a systems biology approach which has unravelled the association/ new processes and thus helped us enormously in understanding of the biological processes. The present book is an attempt at understanding the plant signalling processes with different perspectives. Even though the plants are sessile but there exists a tremendous interconnected network of perception at morphological, physiological and molecular levels. The impact of the surrounding environment in terms of abiotic and biotic stresses is significant in terms of its survival, adaptation and productivity for the human welfare. The plants possess a wide array of processes at the organ, tissue and cellular levels which are governed by a plethora of molecules. The molecules govern individual processes and these exists a cross talk between them to form a complex network of processes. The book tries to envision how different processes are operating at different points in the life cycle of the plant.

Proceedings of the National Academy of Sciences of the United States of America

Author : National Academy of Sciences (U.S.)
Publisher :
ISBN 13 :
Total Pages : 1364 pages
Book Rating : 4.:/5 (891 download)

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Book Synopsis Proceedings of the National Academy of Sciences of the United States of America by : National Academy of Sciences (U.S.)

Download or read book Proceedings of the National Academy of Sciences of the United States of America written by National Academy of Sciences (U.S.) and published by . This book was released on 2006 with total page 1364 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecules to Medicine with mTOR

Author : Kenneth Maiese
Publisher : Academic Press
ISBN 13 : 012802755X
Total Pages : 474 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Molecules to Medicine with mTOR by : Kenneth Maiese

Download or read book Molecules to Medicine with mTOR written by Kenneth Maiese and published by Academic Press. This book was released on 2016-02-21 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecules to Medicine with mTOR: Translating Critical Pathways into Novel Therapeutic Strategies is a one-stop reference that thoroughly covers the mechanistic target of rapamycin (mTOR). mTOR, also known as the mammalian target of rapamycin, is a 289-kDa serine/threonine protein kinase that is ubiquitous throughout the body and has a critical role in gene transcription and protein formation, stem cell development, cell survival and senescence, aging, immunity, tissue regeneration and repair, metabolism, tumorigenesis, oxidative stress, and pathways of programmed cell death that include apoptosis and autophagy. Incorporating a translational medicine approach, this important reference highlights the basic cellular biology of mTOR pathways, presents the role of mTOR during normal physiologic function and disease, and illustrates how the mechanisms of mTOR can be targeted for current and future therapeutic treatment strategies. Coverage of mTOR signaling includes the entire life cycle of cells that impacts multiple systems of the body including those of nervous, cardiovascular, immune, musculoskeletal, endocrine, reproductive, renal, and respiratory origin. Covers the role of mTOR by internationally recognized expert contributors in the field. Provides a clear picture of the complexity of mTOR signaling as well as of the different approaches that could target this pathway at various levels. Includes analysis of the role of mTOR and in both health and disease. Serves as an important resource for a broad audience of healthcare providers, scientists, drug developers, and students in both clinical and research settings.

International Review of Cell and Molecular Biology

Author : Kwang W. Jeon
Publisher : Academic Press
ISBN 13 : 0128004460
Total Pages : 363 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis International Review of Cell and Molecular Biology by : Kwang W. Jeon

Download or read book International Review of Cell and Molecular Biology written by Kwang W. Jeon and published by Academic Press. This book was released on 2014-04-08 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Review of Cell and Molecular Biology presents comprehensive reviews and current advances in cell and molecular biology. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. The series has a world-wide readership, maintaining a high standard by publishing invited articles on important and timely topics authored by prominent cell and molecular biologists. Impact factor for 2012: 4.973. Authored by some of the foremost scientists in the field Provides comprehensive reviews and current advances Wide range of perspectives on specific subjects Valuable reference material for advanced undergraduates, graduate students and professional scientists

Plant signaling: Understanding the molecular crosstalk

Author : Khalid Rehman Hakeem
Publisher : Springer Science & Business Media
ISBN 13 : 8132215427
Total Pages : 355 pages
Book Rating : 4.1/5 (322 download)

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Book Synopsis Plant signaling: Understanding the molecular crosstalk by : Khalid Rehman Hakeem

Download or read book Plant signaling: Understanding the molecular crosstalk written by Khalid Rehman Hakeem and published by Springer Science & Business Media. This book was released on 2013-10-25 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant signalling has emerged as an integrated field which has become indispensable in recent times to study any biological process. Over the last decade, an enormous amount of information has been generated in this field and the advances in information technology gave birth to bioinformatics which has helped greatly in managing the galaxy of information. It is now possible to view the different information’s in a systems biology approach which has unravelled the association/ new processes and thus helped us enormously in understanding of the biological processes. The present book is an attempt at understanding the plant signalling processes with different perspectives. Even though the plants are sessile but there exists a tremendous interconnected network of perception at morphological, physiological and molecular levels. The impact of the surrounding environment in terms of abiotic and biotic stresses is significant in terms of its survival, adaptation and productivity for the human welfare. The plants possess a wide array of processes at the organ, tissue and cellular levels which are governed by a plethora of molecules. The molecules govern individual processes and these exists a cross talk between them to form a complex network of processes. The book tries to envision how different processes are operating at different points in the life cycle of the plant.

Protein Trafficking in Plant Cells

Author : J. Soll
Publisher : Springer Science & Business Media
ISBN 13 : 9780792352372
Total Pages : 410 pages
Book Rating : 4.3/5 (523 download)

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Book Synopsis Protein Trafficking in Plant Cells by : J. Soll

Download or read book Protein Trafficking in Plant Cells written by J. Soll and published by Springer Science & Business Media. This book was released on 1998-08-31 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: The highly structured eucaryotic cell with its complex division of biochemical labour requires a distinct protein complement in each cellular structure and compartment. Nuclear coded and cytosolically synthesized polypeptides are specifically sorted to every corner of the cell in a post- or co-translational manner. The presence of separate genomes and protein translation machineries in plastids and mitochondria requires further coordination not only on the transcriptional, translational but also most likely on the protein import level. Numerous different protein transport systems have developed and coexist within plant cells to ensure the specific and selective composition of every sub-cellular compartment. This volume summarizes the current knowledge on protein trafficking in plant cells. Aside from the fundamental aspects in cell biology of how specific pre-protein sorting and translocation across biological membranes is achieved, a major focus is on transport, modification and deposition of plant storage proteins. The increasing use of plants as bioreactors to provide custom-designed proteins of different usage requires detailed understanding of these events. This text is directed not only at students and professionals in plant cell and molecular biology but also at those involved in horticulture and plant breeding. It is intended to serve as a text and guide for graduate-level courses on plant cell biology and as a valuable supplement to courses in plant physiology and development. Scientists in other disciplines who wish to learn more about protein translocation in plants will also find this text an up-to-date source of information and reference.

Structural Approaches to Sequence Evolution

Author : Ugo Bastolla
Publisher : Springer
ISBN 13 : 9783540353058
Total Pages : 368 pages
Book Rating : 4.3/5 (53 download)

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Book Synopsis Structural Approaches to Sequence Evolution by : Ugo Bastolla

Download or read book Structural Approaches to Sequence Evolution written by Ugo Bastolla and published by Springer. This book was released on 2007-08-17 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in understanding the thermodynamics of macromolecules, the topological properties of gene networks, the organization and mutation capabilities of genomes, and the structure of populations make it possible to incorporate these key elements into a broader and deeply interdisciplinary view of molecular evolution. This book gives an account of such a new approach, through clear tutorial contributions by leading scientists.

Polarity in Plants

Author : K. Lindsey
Publisher : John Wiley & Sons
ISBN 13 : 9780849323447
Total Pages : 368 pages
Book Rating : 4.3/5 (234 download)

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Book Synopsis Polarity in Plants by : K. Lindsey

Download or read book Polarity in Plants written by K. Lindsey and published by John Wiley & Sons. This book was released on 2004 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: The establishment of polarity is a fundamental feature in eukaryotic development. Polarity in Plants provides an account of current research into the mechanisms by which polarity is generated at the level of the cell, organ and organism in plants, drawing especially on recent work with model organisms. The emphasis is on the use of the techniques of molecular genetics to dissect molecular mechanisms. This is the first volume to bring together the diverse aspects of polarity in plant development.

Zebrafish at the Interface of Development and Disease Research

Author :
Publisher : Academic Press
ISBN 13 : 0128033436
Total Pages : 386 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Zebrafish at the Interface of Development and Disease Research by :

Download or read book Zebrafish at the Interface of Development and Disease Research written by and published by Academic Press. This book was released on 2017-03-21 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zebrafish at the Interface of Development and Disease Research presents the latest on the shared pathways that govern development and contribute to disease. Zebrafish have traditionally been used to study vertebrate development, providing interesting data on the developmental processes and genes that are implicated in disease. This new release in the series contains informative discussions on congenital heart defects in zebrafish, the use of zebrafish in studying kidney development and disease, and muscle development, homeostasis and disease in zebrafish. Each chapter interweaves the study of zebrafish development and its application to the immune system, the kidney, liver, heart and others. The subject matter is unique - no single volume has pulled together the theme of development and disease with zebrafish as a centerpiece Leading experts in the field - all of them running zebrafish focused labs for each chapter

Tomosynthesis Imaging

Author : Ingrid Reiser
Publisher : Taylor & Francis
ISBN 13 : 1439878714
Total Pages : 246 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Tomosynthesis Imaging by : Ingrid Reiser

Download or read book Tomosynthesis Imaging written by Ingrid Reiser and published by Taylor & Francis. This book was released on 2016-04-19 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: An innovative, three-dimensional x-ray imaging technique that enhances projection radiography by adding depth resolution, Tomosynthesis Imaging explores tomosynthesis, an emerging limited-angle tomographic imaging technology that is being considered for use in a range of clinical applications, and is currently being used for breast cancer screening

Tunable Hydrogels

Author : Antonina Lavrentieva
Publisher : Springer Nature
ISBN 13 : 3030767698
Total Pages : 256 pages
Book Rating : 4.0/5 (37 download)

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Book Synopsis Tunable Hydrogels by : Antonina Lavrentieva

Download or read book Tunable Hydrogels written by Antonina Lavrentieva and published by Springer Nature. This book was released on 2021-05-31 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the current knowledge on tunable hydrogels, including the range of different materials and applications, as well as the existing challenges and limitations in the field. It covers various aspects of the material design, particularly highlighting biological responsiveness, degradability and responsiveness to external stimuli. In this book, readers will discover original research data and state-of-the-art reviews in the area of hydrogel technology, with a specific focus on biotechnology and medicine. Written by leading experts, the contributions outline strategies for designing tunable hydrogels and offer a detailed evaluation of the physical and synthetic methods currently employed to achieve specific hydrogel properties and responsiveness. This highly informative book provides important theoretical and practical insights for scholars and researchers working with hydrogels for biomedical and biotechnological applications.

Polymeric Delivery Systems

Author : Robert J. Kostelnik
Publisher : Gordon & Breach Publishing Group
ISBN 13 :
Total Pages : 330 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Polymeric Delivery Systems by : Robert J. Kostelnik

Download or read book Polymeric Delivery Systems written by Robert J. Kostelnik and published by Gordon & Breach Publishing Group. This book was released on 1978 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Plant Signal Transduction

Author : Dierk Scheel
Publisher : Oxford University Press, USA
ISBN 13 : 9780199638802
Total Pages : 324 pages
Book Rating : 4.6/5 (388 download)

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Book Synopsis Plant Signal Transduction by : Dierk Scheel

Download or read book Plant Signal Transduction written by Dierk Scheel and published by Oxford University Press, USA. This book was released on 2002 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: The growth and differentiation of living organisms are continuously adjusted to a multitude of environmental factors, each of which underlies a perpetual variation. The sessile existence of plants further emphasizes the requirement for efficient adaptation and defence mechanisms. The changes in environmental factors may range from moderate to dramatic and can concern many components at the same time. They may be abiotic or biotic in nature and range from essential to toxic in their effects. Among the numerous abiotic factors are nutrients, light, oxygen, water, temperature, gravity, wind, touch and chemicals. Biotic factors are represented by other organisms involved in symbiotic, pathogenic or herbivorous interactions with plants. All of these environmental factors are independently and specifically recognized by plants. Perception and overall response are linked by signal transduction pathways at cellular, systemic and interorganismic levels. In order to guarantee proper adaptation to the environment, signals generated following perception of a multitude of environmental factors need to be integrated and evaluated according to their importance. Cross-talk between different signaling pathways within such networks appears to be the basis for the evaluation of the importance of incoming signals. Knowledge of these complex processes allows a better understanding of the molecular mechanisms underlying adaptation. Modulation of distinct signaling elements can generate plants with improved stress resistance. This book represents a comprehensive summary of the enormous amount of information that is now available on signal transduction processes involved in the communication of plants with abiotic and biotic elements of their environment.

Cartilage Regeneration

Author : Yunfeng Lin
Publisher : Humana Press
ISBN 13 : 3319516175
Total Pages : 98 pages
Book Rating : 4.3/5 (195 download)

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Book Synopsis Cartilage Regeneration by : Yunfeng Lin

Download or read book Cartilage Regeneration written by Yunfeng Lin and published by Humana Press. This book was released on 2017-02-21 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on cartilage defects and new mesenchymal stem cell-based treatments for their repair and regeneration. Early chapters provide a review of current etiological findings and repair methods of cartilage defects. The next chapters discuss fundamental concepts and features of MSCs, including their proliferation, differentiation, migration and immunomodulatory effects. The discussion also includes clinical applications of MSCs in cartilage tissues, especially with regards to various animal models, biomaterials and transferring techniques. Cartilage Regeneration focuses on the biology of MSCs and their possible applications in cartilage reconstruction, with the goal of bringing new insights into regenerative medicine. It will be essential reading for researchers and clinicians in stem cells, regenerative medicine, biomedical engineering and orthopedic surgery.