Freeze Etching

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The Nuclear Envelope in Freeze-Etching

Author : J. Kartenbeck
Publisher : Springer Science & Business Media
ISBN 13 : 3662103907
Total Pages : 55 pages
Book Rating : 4.6/5 (621 download)

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Book Synopsis The Nuclear Envelope in Freeze-Etching by : J. Kartenbeck

Download or read book The Nuclear Envelope in Freeze-Etching written by J. Kartenbeck and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past twenty years the structure of the nuclear envelope, and in particular, that of its most distinct elements, the nuclear pore complexes, has been described from thin section electron microscopy (e, g., Brettschneider, 1952; Hartmann, 1953; Bahr and Beermann, 1954; Watson, 1954; Kautz and de Marsh, 1955; Watson, 1955), from metal-shadowed (e. g., Callan and Tomlin, 1950; Gall, 1954, 1956) and negatively stained (e. g., Franke, 1966, 1967; Gall, 1967; Yoo and Bayley, 1967) preparations of isolated nuclear membranes as revealing characte ristics common to euka. ryotic cells in general (recently reviewed, e. g., in Gouran ton, 1969; Stevens and Andre, 1969; Franke, 1970). In the recent years the freeze-etch technique (Steere, 1957) has proved to be a particularly useful tool in studying membraneous structures (e. g., Moor and Miihlethaler, 1963; Branton and Moor, 1964; Branton, 1966; Koehler, 1968b; Staehelin, l968a; Northcote, 1968a; Branton, 1969; Moor, 1969a). So this method has especially broadened the knowledge, e. g., on bacterial membranes (Bayer and Remsen, 1970; Nanninga, 1970), on erythrocyte plasma membranes (Weinstein and Bullivant, 1967; Meyer and Winkelmann, 1970; da Silva and Branton, 1970; Tillack and Marchesi, 1970), on liver cell membranes (Chalcroft and Bullivant, 1970), on Golgi membranes (Werz and Kellner, 1970; Staehelin and Kiermayer, 1970), on synaptic vesicles (Moor et al.

Freeze-Etch Histology

Author : L. Orci
Publisher : Springer Science & Business Media
ISBN 13 : 3642660207
Total Pages : 149 pages
Book Rating : 4.6/5 (426 download)

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Book Synopsis Freeze-Etch Histology by : L. Orci

Download or read book Freeze-Etch Histology written by L. Orci and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 149 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of the electron microscope brought one to the other. The purpose of this book is to in a new era, in which cell structures could be provide a bridge, to help those who are familiar visualized down to a macromolecular level. It is of with conventional electron microscopy, to cross course well recognized that some cell components into the less familiar freeze-etching. This has been can be modified or even completely lost during the done by providing paired photographs wherever complex sequence of fixation, dehydration, staining possible, one showing the thin section of a tissue or and plastic embedding which is essential before a a cell, the other a freeze-etch replica of a similar thin section can be obtained. Further, only these cell material. At the same time we have tried to provide components which can be made electron opaque a basis for interpretation of freeze-etched structures, can be visualized. For these reasons, the morpho and we hope the reader will find the first 23 Plates logist and cell biologist alike have to explore alter especially useful in this respect. Because there are native techniques of specimen preparation which many excellent atlases of cell structure available at may avoid some of the artefacts inherent in con the present time, we felt there was no need to ventional thin-section electron microscopy, and at provide lengthy discussions of each plate.

Freeze Fracture Images of Cells and Tissues

Author : Richard L. Roberts
Publisher : Oxford University Press, USA
ISBN 13 : 9780195052282
Total Pages : 430 pages
Book Rating : 4.0/5 (522 download)

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Book Synopsis Freeze Fracture Images of Cells and Tissues by : Richard L. Roberts

Download or read book Freeze Fracture Images of Cells and Tissues written by Richard L. Roberts and published by Oxford University Press, USA. This book was released on 1991 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: The freeze fracture technique coupled with transmission electron microscopy has contributed significant scientific information toward the experimental investigation of both normal and abnormal cells in the fields of cellular, developmental and molecular biology, and to several subdisciplines in medicine, including pathology, anatomy, and physiology. This book presents a complete and up-to-date account of the macromolecular organization of membranes and the many membrane specialization of cells as well as overall cellular organization as reflected in tissues and organs. While the book emphasizes freeze fracture images and the useful scientific information contained in them, the authors have also included transmission electron micrographs of ultrathin sectioned cells, tissues, and organs in order to aid in the interpretation of the freeze fracture image and increase the book's utility. Where three dimensional views are particularly useful, scanning electron micrographs are included.

Cryotechniques in Biological Electron Microscopy

Author : Rudolf A. Steinbrecht
Publisher : Springer Science & Business Media
ISBN 13 : 3642728154
Total Pages : 308 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Cryotechniques in Biological Electron Microscopy by : Rudolf A. Steinbrecht

Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Rapid Freezing, Freeze Fracture, and Deep Etching

Author : Nicholas J. Severs
Publisher : Wiley-Liss
ISBN 13 :
Total Pages : 392 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Rapid Freezing, Freeze Fracture, and Deep Etching by : Nicholas J. Severs

Download or read book Rapid Freezing, Freeze Fracture, and Deep Etching written by Nicholas J. Severs and published by Wiley-Liss. This book was released on 1995-08-11 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: The techniques of freeze fracture and deep etching—techniques for the examination of the ultrastructure of rapidly frozen biological specimens by transmission electron microscopy—have profoundly influenced our understanding of the functional organization of the cell. Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these techniques and gives detailed examples of their application in biomedical research. The book is organized into three sections. The first covers the fundamentals of freeze fracture and deep etching, with a discussion of basic principles for those new to the field, and an entire chapter on the equipment currently available for freeze fracture. Advanced techniques are presented next, with each chapter devoted to an in-depth treatment of a specific topic, including the advantages and limitations of each technique. The final chapters offer a series of applications selected to illustrate the wide-ranging and enduring impact of rapid freezing, freeze fracture, and deep etching on the field of biomedical research. With over 150 illustrations and containing contributions by leading experts instrumental in the development of these techniques, Rapid Freezing, Freeze Fracture, and Deep Etching offers authoritative coverage of such novel topics as: Hyperbaric freezing Ultrarapid freezing techniques in the study of dynamic cellular processes Cytochemical methods utilizing lectins and antibodies for labeling specific cellcomponents Fracturing and shadowing under ultra-low temperature and ultra-high vacuum conditions Freeze fracture and deep etching in neurobiology Rapid Freezing, Freeze Fracture, and Deep Etching will appeal to both newcomers to the field and experienced researchers and will be essential reading for cell biologists and anatomists, as well as any researcher who uses electron microscopy.

Advanced Techniques in Biological Electron Microscopy

Author : J.K. Koehler
Publisher : Springer Science & Business Media
ISBN 13 : 3642654924
Total Pages : 319 pages
Book Rating : 4.6/5 (426 download)

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Book Synopsis Advanced Techniques in Biological Electron Microscopy by : J.K. Koehler

Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Freeze-Fracture Studies of Membranes

Author : Sek Wen Hui
Publisher : CRC Press
ISBN 13 : 1351080601
Total Pages : 229 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Freeze-Fracture Studies of Membranes by : Sek Wen Hui

Download or read book Freeze-Fracture Studies of Membranes written by Sek Wen Hui and published by CRC Press. This book was released on 2018-01-18 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.

Methods in Neurobiology

Author : Robert Lahue
Publisher : Springer Science & Business Media
ISBN 13 : 1468438069
Total Pages : 607 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Methods in Neurobiology by : Robert Lahue

Download or read book Methods in Neurobiology written by Robert Lahue and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in knowledge have led to an increasing interest in neuro biology over the last several years. These advances have been made possible, at least in part, by the use of increasingly sophisticated methodology. Furthermore, research in the most rapidly advancing areas is essentially multidisciplinary and is characterized by contributions from many investi gators employing a variety of techniques. While a grasp of fundamental neurobiological concepts is an obvious prerequisite for those who wish to follow or participate in this field, critical awareness and evaluation of neurobiological research also requires an understanding of sophisticated methodologies. The objective of Methods in Neurobiology is the development of such critical abilities. The reader is exposed to the basic concepts, principles, and instrumentation of key methodologies, and the application of each meth odology is placed in the special context of neurobiological research. The reader will gain familiarity with the terminology and procedures of each method and the ability to evaluate results in light of the particular features of neurobiological preparations and applications.

Low-Temperature Microscopy and Analysis

Author : Patrick Echlin
Publisher : Springer Science & Business Media
ISBN 13 : 1489923020
Total Pages : 553 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis Low-Temperature Microscopy and Analysis by : Patrick Echlin

Download or read book Low-Temperature Microscopy and Analysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.

Methods in Microbiology

Author :
Publisher : Academic Press
ISBN 13 : 0080860389
Total Pages : 268 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Methods in Microbiology by :

Download or read book Methods in Microbiology written by and published by Academic Press. This book was released on 1976-11-24 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Microbiology

Advances in Biological and Medical Physics

Author : John H. Lawrence
Publisher : Academic Press
ISBN 13 : 1483224317
Total Pages : 391 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Advances in Biological and Medical Physics by : John H. Lawrence

Download or read book Advances in Biological and Medical Physics written by John H. Lawrence and published by Academic Press. This book was released on 2013-10-22 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biological and Medical Physics, Volume 12 covers the significant progress in various aspects of biology and medical physics. This volume is composed of 16 chapters. The opening chapters deal with the principles and application of freeze-etching technique and scanning electron microscopy. The succeeding chapters review the development rudimentary model of the chromosome, the mechanisms involving large number of steps to kinetic studies of multisubstrate enzyme systems, and some biophysical approaches to evaluate radiation effects and their repair. These topics are followed by discussions the origin of the observed fluorescence and phosphorescence spectra of DNA, as well as some aspects of energy transfer that apply to DNA and other polynucleotides. Other chapters explore the processes of cellular repair, cell's radiation sensivity, bacterial photoreactivation of mutation, and the genetic control of DNA repair and genetic recombination. The final chapters consider the mechanism of mutation suppression in yeast, the role of cytoplasm in radiobiology, and the different random factors governing the dose-effect relation. This book is of value to biologists, medical physicists, and medical practitioners.

Cumulated Index Medicus

Author :
Publisher :
ISBN 13 :
Total Pages : 1348 pages
Book Rating : 4.3/5 ( download)

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Book Synopsis Cumulated Index Medicus by :

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1974 with total page 1348 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Electron Microscopy of Model Systems

Author :
Publisher : Academic Press
ISBN 13 : 0123810086
Total Pages : 745 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Electron Microscopy of Model Systems by :

Download or read book Electron Microscopy of Model Systems written by and published by Academic Press. This book was released on 2010-09-24 with total page 745 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. - Covers the preparation and analysis of model systems for biological electron microscopy - Includes the most popular systems but also organisms that are less frequently used in cell biology - Presents the currently most important methods for the preparation of biological specimens - First compendium covering the various aspects of sample preparation of very diverse biological systems

Ultrastructure Techniques for Microorganisms

Author : H.C. Aldrich
Publisher : Springer Science & Business Media
ISBN 13 : 1468451197
Total Pages : 540 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Ultrastructure Techniques for Microorganisms by : H.C. Aldrich

Download or read book Ultrastructure Techniques for Microorganisms written by H.C. Aldrich and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.

Electron Microscopy of Plant Cells

Author : C Hawes
Publisher : Academic Press
ISBN 13 : 0323148980
Total Pages : 477 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Electron Microscopy of Plant Cells by : C Hawes

Download or read book Electron Microscopy of Plant Cells written by C Hawes and published by Academic Press. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

Elsevier's Dictionary of Medicine and Biology

Author : G. Konstantinidis
Publisher : Elsevier
ISBN 13 : 0080460127
Total Pages : 2590 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Elsevier's Dictionary of Medicine and Biology by : G. Konstantinidis

Download or read book Elsevier's Dictionary of Medicine and Biology written by G. Konstantinidis and published by Elsevier. This book was released on 2005-08-18 with total page 2590 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dictionaries are didactic books used as consultation instruments for self-teaching. They are composed by an ordered set of linguistic units which reflects a double structure, the macrostructure which correspond to the word list and the microstructure that refers to the contents of each lemma. The great value of dictionaries nests in the fact that they establish a standard nomenclature and prevent in that way the appearance of new useless synonyms.This dictionary contains a total of about 27.500 main English entries, and over of 130.000 translations that should normally sufficiently cover all fields of life sciences. The basic criteria used to accept a word a part of the dictionary during the development period in order of importance were usage, up-to-dateness, specificity, simplicity and conceptual relationships. The dictionary meets the standards of higher education and covers all main fields of life sciences by setting its primary focus on the vastly developing fields of cell biology, biochemistry, molecular biology, immunology, developmental biology, microbiology, genetics and also the fields of human anatomy, histology, pathology, physiology, zoology and botany.The fields of ecology, paleontology, systematics, evolution, biostatistics, plant physiology, plant anatomy, plant histology, biometry and lab techniques have been sufficiently covered but in a more general manner. The latest Latin international anatomical terminology "Terminologia Anatomica" or "TA" has been fully incorporated and all anatomical entries have been given their international Latin TA synonym. This dictionary will be a valuable and helpful tool for all scientists, teachers, students and generally all those that work within the fields of life sciences.

Polymer Microscopy

Author : Linda C. Sawyer
Publisher : Springer Science & Business Media
ISBN 13 : 9400931395
Total Pages : 318 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Polymer Microscopy by : Linda C. Sawyer

Download or read book Polymer Microscopy written by Linda C. Sawyer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern materials include a vast array of polymers and plastics which are found in applications such as housing, appliances, clothing and household textiles and automotive and aerospace industries. Thus research scientists, engineers and materials science graduate students need to be aware of the methods and techniques required to understand the structure-property relations of polymer materials. This book will review the field of the microscopy of polymers. There is a vast literature which describes the research results obtained by study of polymer materials using microscopy and other complementary analytical techniques and such studies are best left to journals on specific topics. The major objective of this text is to provide the basic microscopy techniques and specimen preparation methods applicable to polymers. The book will attempt to provide enough detail so that the methods described can be applied, and also to reference appropriate publications for the investigator interested in more detail. The selection of two authors for this text came from the desire for a comprehen sive review of polymer microscopy with emphasis on methods and techniques, rather than on research results. The synergism provided by two authors with widely varied backgrounds was thought to be important: one author (LCS) has an industrial focus and a background in chemistry whilst the other (DTG) has an academic environment and offers a background in polymer physics.