Fplc Fast Protein Liquid Chromatography

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Protein Chromatography

Author : Dermot Walls
Publisher : Humana Press
ISBN 13 : 9781607619147
Total Pages : 527 pages
Book Rating : 4.6/5 (191 download)

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Book Synopsis Protein Chromatography by : Dermot Walls

Download or read book Protein Chromatography written by Dermot Walls and published by Humana Press. This book was released on 2011-04-06 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular BiologyTM series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.

Protein Purification Protocols

Author : Paul Cutler
Publisher : Springer Science & Business Media
ISBN 13 : 159259655X
Total Pages : 474 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Protein Purification Protocols by : Paul Cutler

Download or read book Protein Purification Protocols written by Paul Cutler and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

Protein Purification Protocols

Author : Shawn Doonan
Publisher : Springer Science & Business Media
ISBN 13 : 1592595456
Total Pages : 406 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Protein Purification Protocols by : Shawn Doonan

Download or read book Protein Purification Protocols written by Shawn Doonan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased interest in proteins as gene products. Hence, many laborato ries are now getting involved in protein isolation for the first time, either as an essential adjunct to their work in molecular genetics or because of a curiosity to know more about the products of the genes that they have been studying. Protein Purification Protocols is aimed mainly at these newcom ers to protein purification, but it is hoped that it will also be of value to established practitioners who may find here techniques that they have not tried, but which might well be most applicable in their work. With the exception mainly of the first and last chapters, the format of the contributions to the present book conform to the established format of the Methods in Molecular Biology series.

Chapters In The Evolution Of Chromatography

Author : Leslie S Ettre
Publisher : World Scientific
ISBN 13 : 1908979054
Total Pages : 492 pages
Book Rating : 4.9/5 (89 download)

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Book Synopsis Chapters In The Evolution Of Chromatography by : Leslie S Ettre

Download or read book Chapters In The Evolution Of Chromatography written by Leslie S Ettre and published by World Scientific. This book was released on 2008-06-11 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography, invented more than 100 years ago, is the most widely used separation technique in the world today. It has helped the birth of modern analytical instrumentation and continues to strongly influence the profiles of our chemical, biochemical and clinical laboratories.This book deals with the history of the invention and evolution of chromatography and of the various chromatographic techniques. After discussing the precursors, it elaborates on the activities of M.S. Tswett, the inventor of the technique, and of a few selected key pioneers. It then summarizes the evolution of the various branches of chromatography (planar, ion-exchange, gas and liquid), and also reviews the key role of international symposia in setting the trends in this evolution. Except for individual publications of the author, the history of the evolution of chromatography has not been the subject of any book. Thus, this book fills a major gap in the scientific literature./a

HPLC and FPLC: Troubleshooting and Standardizing Chromatogram Purification Profiles

Author : T. S. Amar Anand Rao
Publisher : GRIN Verlag
ISBN 13 : 3656103232
Total Pages : 105 pages
Book Rating : 4.6/5 (561 download)

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Book Synopsis HPLC and FPLC: Troubleshooting and Standardizing Chromatogram Purification Profiles by : T. S. Amar Anand Rao

Download or read book HPLC and FPLC: Troubleshooting and Standardizing Chromatogram Purification Profiles written by T. S. Amar Anand Rao and published by GRIN Verlag. This book was released on 2012 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Study from the year 2012 in the subject Biology - Micro- and Molecular Biology, Indian Institute of Science, course: Molecular Biology, language: English, abstract: This is a tool to separate compounds according to its hydrophobicity or hydrophilicity. A gradient is given from hydrophilic to hydrophobic range of solvent moving through a solid matrix. Here are some valuable tips collected through experience and handed over so that the reader can find it useful when the chromatograms and the instruments prove so cross with you. The instrumentation and working for both the systems HPLC and FPLC are almost same. HPLC is High Performance/Pressure Liquid Chromatography and FPLC is Fast Protein Liquid Chromtography.

Process Scale Purification of Antibodies

Author : Uwe Gottschalk
Publisher : John Wiley & Sons
ISBN 13 : 1119126932
Total Pages : 849 pages
Book Rating : 4.1/5 (191 download)

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Book Synopsis Process Scale Purification of Antibodies by : Uwe Gottschalk

Download or read book Process Scale Purification of Antibodies written by Uwe Gottschalk and published by John Wiley & Sons. This book was released on 2017-03-07 with total page 849 pages. Available in PDF, EPUB and Kindle. Book excerpt: Promoting a continued and much-needed renaissance in biopharmaceutical manufacturing, this book covers the different strategies and assembles top-tier technology experts to address the challenges of antibody purification. • Updates existing topics and adds new ones that include purification of antibodies produced in novel production systems, novel separation technologies, novel antibody formats and alternative scaffolds, and strategies for ton-scale manufacturing • Presents new and updated discussions of different purification technologies, focusing on how they can address the capacity crunch in antibody purification • Emphasizes antibodies and innovative chromatography methods for processing

Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms

Author : Horacio G Pontis
Publisher : Academic Press
ISBN 13 : 0128034033
Total Pages : 252 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms by : Horacio G Pontis

Download or read book Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms written by Horacio G Pontis and published by Academic Press. This book was released on 2016-09-06 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms. The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available. - Includes a variety of analytical methods and how to apply the methods using examples from specific case studies - Discusses technical information on how to characterize plant carbohydrates and sugar nucleosides - Contains easy-to-follow protocols with detailed explanations for self-guidance - Provides foundational content as well as step-by-step guidance on how to design and conduct an experiment

Proteins in Food Processing

Author : Rickey Y. Yada
Publisher : Woodhead Publishing
ISBN 13 : 0081007299
Total Pages : 672 pages
Book Rating : 4.0/5 (81 download)

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Book Synopsis Proteins in Food Processing by : Rickey Y. Yada

Download or read book Proteins in Food Processing written by Rickey Y. Yada and published by Woodhead Publishing. This book was released on 2017-11-13 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins in Food Processing, Second Edition, reviews how proteins may be used to enhance the nutritional, textural and other qualities of food products. After two introductory chapters, the book discusses sources of proteins, examining the caseins, whey, muscle and soy proteins, and proteins from oil-producing plants, cereals and seaweed. Part Two illustrates the analysis and modification of proteins, with chapters on testing protein functionality, modeling protein behavior, extracting and purifying proteins and reducing their allergenicity. A final group of chapters delves into the functional value of proteins and how they are used as additives in foods. - Completely revised and updated with new developments on all food protein analysis and applications, such as alternative proteins sources, proteins as emulsifiers, proteins in nanotechnology and egg proteins - Reviews the wide range of protein sources available - Examines ways of modifying protein sources - Discusses the use of proteins to enhance the nutritional, textural and other qualities of food products

Branched-chain Amino Acids

Author : Robert Allison Harris
Publisher : Academic Press
ISBN 13 : 0121822257
Total Pages : 587 pages
Book Rating : 4.1/5 (218 download)

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Book Synopsis Branched-chain Amino Acids by : Robert Allison Harris

Download or read book Branched-chain Amino Acids written by Robert Allison Harris and published by Academic Press. This book was released on 2000 with total page 587 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.

Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics

Author : Allan Matte
Publisher : Elsevier
ISBN 13 : 0081030193
Total Pages : 222 pages
Book Rating : 4.0/5 (81 download)

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Book Synopsis Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics by : Allan Matte

Download or read book Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics written by Allan Matte and published by Elsevier. This book was released on 2020-09-07 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography

Principles and Practice of Modern Chromatographic Methods

Author : Kevin Robards
Publisher : Academic Press
ISBN 13 : 012822097X
Total Pages : 532 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Principles and Practice of Modern Chromatographic Methods by : Kevin Robards

Download or read book Principles and Practice of Modern Chromatographic Methods written by Kevin Robards and published by Academic Press. This book was released on 2021-12-03 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Practice of Modern Chromatographic Methods, Second Edition takes a comprehensive, unified approach in its presentation of chromatographic techniques. Like the first edition, the book provides a scientifically rigid, but easy-to-follow presentation of chromatography concepts that begins with the purpose and intent of chromatographic theory - the "what and why that are left out of other books attempting to cover these principles. This fully revised second edition brings the content up-to-date, covering recent developments in several new sections and an additional chapter on composite methods. New topics include sample profiling, sample preparation, sustainable green chemistry, 2D chromatography, miniaturization/nano-LC, HILIC, and more. - Contains thorough chapters that begin with an updated schematic overview and a visual representation of the content - Avoids the obfuscation of different terminologies and classification systems that are prevalent in the area, such as the relationship between liquid chromatography and column chromatography - Provides integrated and comprehensive topic coverage based on chromatographic bibliometrics and survey reports on the relative usage of chromatographic techniques

Biophysical Chemistry of Proteins

Author : Engelbert Buxbaum
Publisher : Springer Science & Business Media
ISBN 13 : 144197251X
Total Pages : 490 pages
Book Rating : 4.4/5 (419 download)

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Book Synopsis Biophysical Chemistry of Proteins by : Engelbert Buxbaum

Download or read book Biophysical Chemistry of Proteins written by Engelbert Buxbaum and published by Springer Science & Business Media. This book was released on 2010-11-18 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is structured in nine sections, each containing several chapters. The volume starts with an overview of analytical techniques and progresses through purification of proteins; protein modification and inactivation; protein size, shape, and structure; enzyme kinetics; protein-ligand interactions; industrial enzymology; and laboratory quality control. The book is targeted at all scientists interested in protein research.

Protein Purification

Author : Jan-Christer Janson
Publisher : John Wiley & Sons
ISBN 13 : 9780471186267
Total Pages : 712 pages
Book Rating : 4.1/5 (862 download)

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Book Synopsis Protein Purification by : Jan-Christer Janson

Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.

HPLC

Author : Marvin C. McMaster
Publisher : John Wiley & Sons
ISBN 13 : 0471754013
Total Pages : 252 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis HPLC by : Marvin C. McMaster

Download or read book HPLC written by Marvin C. McMaster and published by John Wiley & Sons. This book was released on 2006-12-18 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Second Edition of the classic handbook details how to set up an HPLC system that capitalizes on the latest innovations. It covers new techniques in high-temperature, micro-flow, and ultra-fast chromatography, the linking of an HPLC to a mass spectrometer, and more. Complete appendices and supplementary material online, this guide has everything chromatographers need to know to confidently separate, identify, purify, and quantify compounds.

Modern Size-Exclusion Liquid Chromatography

Author : Andre Striegel
Publisher : John Wiley & Sons
ISBN 13 : 0470442832
Total Pages : 512 pages
Book Rating : 4.4/5 (74 download)

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Book Synopsis Modern Size-Exclusion Liquid Chromatography by : Andre Striegel

Download or read book Modern Size-Exclusion Liquid Chromatography written by Andre Striegel and published by John Wiley & Sons. This book was released on 2009-07-31 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

HPLC of Peptides and Proteins

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
ISBN 13 : 1592597424
Total Pages : 411 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis HPLC of Peptides and Proteins by : Marie-Isabel Aguilar

Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

HPLC and UHPLC for Practicing Scientists

Author : Michael W. Dong
Publisher : John Wiley & Sons
ISBN 13 : 1119313767
Total Pages : 410 pages
Book Rating : 4.1/5 (193 download)

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Book Synopsis HPLC and UHPLC for Practicing Scientists by : Michael W. Dong

Download or read book HPLC and UHPLC for Practicing Scientists written by Michael W. Dong and published by John Wiley & Sons. This book was released on 2019-07-23 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.