Fluorescence Spectroscopy

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Principles of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0387463127
Total Pages : 961 pages
Book Rating : 4.3/5 (874 download)

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Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2007-12-05 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Handbook of Fluorescence Spectroscopy and Imaging

Author : Markus Sauer
Publisher : John Wiley & Sons
ISBN 13 : 3527633529
Total Pages : 425 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Handbook of Fluorescence Spectroscopy and Imaging by : Markus Sauer

Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Principles and Applications of Fluorescence Spectroscopy

Author : Jihad Rene Albani
Publisher : John Wiley & Sons
ISBN 13 : 0470691336
Total Pages : 264 pages
Book Rating : 4.4/5 (76 download)

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Book Synopsis Principles and Applications of Fluorescence Spectroscopy by : Jihad Rene Albani

Download or read book Principles and Applications of Fluorescence Spectroscopy written by Jihad Rene Albani and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence Spectroscopy gives the student and new user the essential information to help them to understand and use the technique confidently in their research. By integrating the treatment of absorption and fluorescence, the student is shown how fluorescence phenomena arise and how these can be used to probe a range of analytical problems. A key element of the book is the inclusion of practical laboratory experiments that illustrate the fundamental points and applications of the technique.

New Trends in Fluorescence Spectroscopy

Author : Bernard Valeur
Publisher : Springer Science & Business Media
ISBN 13 : 364256853X
Total Pages : 496 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis New Trends in Fluorescence Spectroscopy by : Bernard Valeur

Download or read book New Trends in Fluorescence Spectroscopy written by Bernard Valeur and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

X-Ray Fluorescence Spectroscopy for Laboratory Applications

Author : Michael Haschke
Publisher : John Wiley & Sons
ISBN 13 : 3527344632
Total Pages : 496 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis X-Ray Fluorescence Spectroscopy for Laboratory Applications by : Michael Haschke

Download or read book X-Ray Fluorescence Spectroscopy for Laboratory Applications written by Michael Haschke and published by John Wiley & Sons. This book was released on 2021-04-05 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides comprehensive coverage on using X-ray fluorescence for laboratory applications This book focuses on the practical aspects of X-ray fluorescence (XRF) spectroscopy and discusses the requirements for a successful sample analysis, such as sample preparation, measurement techniques and calibration, as well as the quality of the analysis results. X-Ray Fluorescence Spectroscopy for Laboratory Applications begins with a short overview of the physical fundamentals of the generation of X-rays and their interaction with the sample material, followed by a presentation of the different methods of sample preparation in dependence on the quality of the source material and the objective of the measurement. After a short description of the different available equipment types and their respective performance, the book provides in-depth information on the choice of the optimal measurement conditions and the processing of the measurement results. It covers instrument types for XRF; acquisition and evaluation of X-Ray spectra; analytical errors; analysis of homogeneous materials, powders, and liquids; special applications of XRF; process control and automation. An important resource for the analytical chemist, providing concrete guidelines and support for everyday analyses Focuses on daily laboratory work with commercially available devices Offers a unique compilation of knowledge and best practices from equipment manufacturers and users Covers the entire work process: sample preparation, the actual measurement, data processing, assessment of uncertainty, and accuracy of the obtained results X-Ray Fluorescence Spectroscopy for Laboratory Applications appeals to analytical chemists, analytical laboratories, materials scientists, environmental chemists, chemical engineers, biotechnologists, and pharma engineers.

Handbook of Single Molecule Fluorescence Spectroscopy

Author : Chris Gell
Publisher : Oxford University Press on Demand
ISBN 13 : 0198529422
Total Pages : 279 pages
Book Rating : 4.1/5 (985 download)

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Book Synopsis Handbook of Single Molecule Fluorescence Spectroscopy by : Chris Gell

Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by Oxford University Press on Demand. This book was released on 2006-08-17 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Practical Fluorescence Spectroscopy

Author : Zygmunt (Karol) Gryczynski
Publisher : CRC Press
ISBN 13 : 1439821704
Total Pages : 793 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Practical Fluorescence Spectroscopy by : Zygmunt (Karol) Gryczynski

Download or read book Practical Fluorescence Spectroscopy written by Zygmunt (Karol) Gryczynski and published by CRC Press. This book was released on 2019-12-18 with total page 793 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Advanced Concepts in Fluorescence Sensing

Author : Chris D. Geddes
Publisher : Springer Science & Business Media
ISBN 13 : 9780387233345
Total Pages : 346 pages
Book Rating : 4.2/5 (333 download)

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Book Synopsis Advanced Concepts in Fluorescence Sensing by : Chris D. Geddes

Download or read book Advanced Concepts in Fluorescence Sensing written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2005-06-28 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Biophysical and Biochemical Aspects of Fluorescence Spectroscopy

Author : T.G. Dewey
Publisher : Springer Science & Business Media
ISBN 13 : 1475795130
Total Pages : 305 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Biophysical and Biochemical Aspects of Fluorescence Spectroscopy by : T.G. Dewey

Download or read book Biophysical and Biochemical Aspects of Fluorescence Spectroscopy written by T.G. Dewey and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy has traditionally found wide application in bio chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex ploited with great success utilizing both intrinsic and extrinsic probes. Re cent applications have built upon these traditional strengths and have re sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of fundamental processes. Frequently, fluorescence techniques are tailored to attack a specific biological problem. These new methods in turn produce new physical situations and phenomena which are often of interest to the physical chemist. Thus, progress in one area stimulates renewed interest in other areas. The goal of this book is to provide detailed monographs on the use of fluorescence to investigate problems at the forefront of biochemistry and cell biology. This book is not meant to be a comprehensive survey but rather to highlight areas of recent developments. It is designed to be readable to the novice and yet provide sufficient detail for the expert to keep abreast of recent developments. The book is organized so that it proceeds from simple biochemical sys tems to more complex cell biological ones. Chapter I on fluorescence quenching of biological structures is a good introductory chapter. It intro duces a number of elementary concepts and discusses applications to pro teins and biomembranes.

Introduction to Fluorescence Spectroscopy

Author : Ashutosh Sharma
Publisher : Wiley-Interscience
ISBN 13 :
Total Pages : 200 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Introduction to Fluorescence Spectroscopy by : Ashutosh Sharma

Download or read book Introduction to Fluorescence Spectroscopy written by Ashutosh Sharma and published by Wiley-Interscience. This book was released on 1999-05-21 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: An accessible guide to all aspects of molecular fluorescence spectroscopy This book introduces the uninitiated reader to the growing body of analytical methods based on molecular fluorescence. Geared to practitioners with no particular training or exposure to the field, it highlights fluorescence spectroscopy's tremendous appeal in present-day pharmaceutical, biomedical, and environmental analysis. Written by two highly respected experts in the field, Introduction to Fluorescence Spectroscopy covers all aspects of the technology-physical fundamentals, instrumentation, methods, and applications. The information is offered at 0a very practical level and addresses a broad range of chemical, physical, biological, and geological problems. The authors incorporate recent advances in commercially available instrumentation as well as fluorescent derivatizing agents, provide many examples of state-of-the-art applications, and discuss future trends. Concise, accessible, up-to-date, Introduction to Fluorescence Spectroscopy is an indispensable reference and an invaluable primer for those involved in the field of analytical science and other professionals interested in this fast-evolving analytical technique.

An Introduction to Fluorescence Correlation Spectroscopy

Author : Thorsten Wohland
Publisher : Myprint
ISBN 13 : 9780750320818
Total Pages : 368 pages
Book Rating : 4.3/5 (28 download)

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Book Synopsis An Introduction to Fluorescence Correlation Spectroscopy by : Thorsten Wohland

Download or read book An Introduction to Fluorescence Correlation Spectroscopy written by Thorsten Wohland and published by Myprint. This book was released on 2020-11-05 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, and potential pitfalls as well as ways to avoid them. This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting. Key Features Introduces the concepts of FCS in an accessible way, supported by animations and graphics in the ebook. Includes a supplementary interactive computable document file that allows the reader to experiment with various FCS setup and fit parameters, allowing readers to test their understanding and simulate experimental outcomes. Provides rigorous mathematical derivations of fundamental FCS equations and models. Pedagogical features include questions, short reviews and critical discussions of literature relevant to the particular chapter that include applications and fundamental developments in the field of FCS.

Fluorescence Spectroscopy in Biology

Author : Martin Hof
Publisher : Springer Science & Business Media
ISBN 13 : 9783540223382
Total Pages : 330 pages
Book Rating : 4.2/5 (233 download)

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Book Synopsis Fluorescence Spectroscopy in Biology by : Martin Hof

Download or read book Fluorescence Spectroscopy in Biology written by Martin Hof and published by Springer Science & Business Media. This book was released on 2004-12-08 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology

Author : R. Cundall
Publisher : Springer Science & Business Media
ISBN 13 : 1475716346
Total Pages : 767 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology by : R. Cundall

Download or read book Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology written by R. Cundall and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 767 pages. Available in PDF, EPUB and Kindle. Book excerpt: At the time that the editors conceived the idea of trying to organize the meeting on which the contents of this volume are based and which became, in March 1980, a NATO Advanced Study Institute, the techniques of time-resolved fluorescence spectroscopy, in both the nanosecond and sub-nanosecond time-domains, might reasonably have been said to be coming of age, both in their execution and in the analysis and interpretation of the results obtained. These techniques, then as now, comprised mainly a number of pulse methods using laser, flash-lamp or, most recently, synchrotron radiation. In addition, significant developments in the more classical phase approach had also rendered that method popular, utilizing either modulation of an otherwise continuous source or, again recently, the ultra-rapid pulse rate attainable with a synchrotron source. In general terms, time-resolved fluorescence studies are capable, under appropriate conditions, of supplying direct kinetic information on both photophysics and various aspects of molecular, macromolecular and supramolecular structure and dynamics. The nanosecond and sub-nanosecond time-scales directly probed render these techniques particularly appropriate in studying relaxation and fluctuation processes in macromolecules, particularly biopolymers (e. g. proteins, nucleic acids), in supramolecular assemblies such as cell membranes, and in a variety of relatively simpler model systems.

Laboratory Micro-X-Ray Fluorescence Spectroscopy

Author : Michael Haschke
Publisher : Springer Science & Business Media
ISBN 13 : 3319048643
Total Pages : 367 pages
Book Rating : 4.3/5 (19 download)

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Book Synopsis Laboratory Micro-X-Ray Fluorescence Spectroscopy by : Michael Haschke

Download or read book Laboratory Micro-X-Ray Fluorescence Spectroscopy written by Michael Haschke and published by Springer Science & Business Media. This book was released on 2014-05-08 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micro-X-ray fluorescence offers the possibility for a position- sensitive and non-destructive analysis that can be used for the analysis of non-homogeneous materials and layer systems. This analytical technique has shown a dynamic development in the last 15 years and is used for the analysis of small particles, inclusions, of elemental distributions for a wide range of different applications both in research and quality control. The first experiments were performed on synchrotrons but there is a requirement for laboratory instruments which offers a fast and immediate access for analytical results. The book discuss the main components of a μ-XRF instrument and the different measurement modes, it gives an overview about the various instruments types, considers the special requirements for quantification of non-homogeneous materials and presents a wide range of application for single point and multi-point analysis as well as for distribution analysis in one, two and three dimensions.

Fluorescence Correlation Spectroscopy

Author : R. Rigler
Publisher : Springer Science & Business Media
ISBN 13 : 3642595421
Total Pages : 503 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Fluorescence Correlation Spectroscopy by : R. Rigler

Download or read book Fluorescence Correlation Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book-length treatment of both the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. The high spatial and temporal resolution of FCS has made it a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion, and flow. It contains an essential contribution from Nobel Prize winner M. Eigen, who is credited with inventing FCS.

Molecular Fluorescence

Author : Bernard Valeur
Publisher : John Wiley & Sons
ISBN 13 : 3527328467
Total Pages : 595 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Molecular Fluorescence by : Bernard Valeur

Download or read book Molecular Fluorescence written by Bernard Valeur and published by John Wiley & Sons. This book was released on 2013-03-25 with total page 595 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

Fluorescence Lifetime Spectroscopy and Imaging

Author : Laura Marcu
Publisher : CRC Press
ISBN 13 : 1439861684
Total Pages : 554 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Fluorescence Lifetime Spectroscopy and Imaging by : Laura Marcu

Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia